{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T15:42:45Z","timestamp":1792942965651,"version":"4.5.0"},"reference-count":61,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Salinity stress significantly restricts crop productivity by impairing germination and early seedling growth through osmotic, ionic, and oxidative damage. The present study evaluated whether sodium hydrosulfide (NaHS) priming confers greater protection than hydropriming in B. napus (Brassica napus L). Seeds were primed with water, 0.1 mM NaHS, or 10 mM NaHS and then germinated under saline conditions. Parameters measured included germination rate, root length, endogenous hydrogen sulfide (H2S) content, antioxidant capacity, oxidative stress markers, and membrane integrity. Salinity reduced germination to 30% and root length to 1.6 mm in unprimed seeds. Both 0.1 mM and 10 mM NaHS priming produced more substantial improvements in these parameters compared to hydropriming. The most pronounced effect was observed with 10 mM NaHS, which after 48 h increased germination to 100% and root length to 30 mm. Furthermore, 10 mM NaHS priming most effectively elevated endogenous H2S levels, enhanced antioxidant capacity (60.43 \u00b5M TE\/g FW at 24 h), reduced hydrogen peroxide (H2O2) (2.04 nmol g\u22121 FW at 48 h) and thiobarbituric acid reactive substances (TBARS) (0.008 mmol g\u22121 FW at 48 h), and preserved membrane integrity by limiting Evans staining and electrolyte leakage. In summary, NaHS priming provided substantially greater mitigation of salinity-induced damage than hydropriming in B. napus.<\/jats:p>","DOI":"10.3390\/plants15040551","type":"journal-article","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T09:16:08Z","timestamp":1770801368000},"page":"551","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative Effects of Hydropriming and NaHS-Priming on Salinity Tolerance in Brassica napus L. Seedlings"],"prefix":"10.3390","volume":"15","author":[{"given":"Faezeh","family":"Bazvand","sequence":"first","affiliation":[{"name":"Department of Plant Production Engineering and Genetics, Faculty of Agriculture, Lorestan University, Khorramabad 68151-44316, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7054-9723","authenticated-orcid":false,"given":"\u0141ukasz","family":"Wojtyla","sequence":"additional","affiliation":[{"name":"Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Poznan, ul. Uniwersytetu Poznanskiego 6, 61-614 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4473-049X","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Adamiec","sequence":"additional","affiliation":[{"name":"Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Poznan, ul. Uniwersytetu Poznanskiego 6, 61-614 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6408-5849","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Garnczarska","sequence":"additional","affiliation":[{"name":"Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Poznan, ul. Uniwersytetu Poznanskiego 6, 61-614 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102671","DOI":"10.1016\/j.pbi.2024.102671","article-title":"Current progress in deciphering the molecular mechanisms underlying plant salt tolerance","volume":"83","author":"Hu","year":"2025","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1007\/s00344-022-10591-8","article-title":"Molecular Insights into the Role of Reactive Oxygen, Nitrogen and Sulphur Species in Conferring Salinity Stress Tolerance in Plants","volume":"42","author":"Mangal","year":"2023","journal-title":"J. Plant Growth Regul."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Hasanuzzaman, M., Hakeem, K.R., Nahar, K., and Alharby, H.F. (2019). 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