{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T06:58:26Z","timestamp":1782543506224,"version":"3.54.5"},"reference-count":139,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Federal State Budgetary Scientific Institution Federal Research Center","award":["FGEM-2022-0005"],"award-info":[{"award-number":["FGEM-2022-0005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>For the development of effective and secure methods for plant genetic resources preservation, different storage treatments of fiber flax seeds were compared. Seeds of the flax variety Orshanskiy-2 in aluminum foil bags were stored at different low temperatures, including in liquid nitrogen. Agronomic characters of plants grown from them and next-generation seeds were compared. Plants grown from frozen seeds changed 14 out of 31 evaluated characters in comparison with the non-frozen control. The biggest changes were detected after gradual freezing in liquid nitrogen, due to mechanical damage of the seed coat, and storage at \u221210 \u00b0C for 24 years. Freezing had a negative effect on production characters (straw, fiber and seed) because of the reduction of the germinated plant number. Seeds stored for 24 years at \u221210 \u00b0C, compared to control plants, ripened earlier, grew higher, produced a greater yield of straw and fiber, but had reduced fiber quality and increased seed size. Plants of the next generation showed a tendency toward attenuation of the storage time influence on flax characters. However, it is unknown how many years this process will take. For seed preservation in GeneBanks, it is recommended to use several variants of storage conditions and use rapid cooling and\/or cryoprotectors. The latter two methods, which have been successfully used for other crops, should be implemented only after preliminary experiments.<\/jats:p>","DOI":"10.3390\/plants15040602","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T16:09:32Z","timestamp":1770998972000},"page":"602","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Influence of Cryopreservation and Low-Temperature Seed Storage on the Morphological and Agronomical Characteristics of Fiber Flax"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5098-4904","authenticated-orcid":false,"given":"Andrey V.","family":"Pavlov","sequence":"first","affiliation":[{"name":"Department of Oil and Fiber Crops, N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 42\u201344 B. Morskaya Str., 190000 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8328-9684","authenticated-orcid":false,"given":"Elizaveta A.","family":"Porokhovinova","sequence":"additional","affiliation":[{"name":"Department of Oil and Fiber Crops, N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 42\u201344 B. Morskaya Str., 190000 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4319-0353","authenticated-orcid":false,"given":"Aleksandr V.","family":"Pavlov","sequence":"additional","affiliation":[{"name":"Laboratory of Long-Term Plants Genetic Resources Preservation, N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 42\u201344 B. Morskaya Str., 190000 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3892-9873","authenticated-orcid":false,"given":"Irina V.","family":"Kiseleva","sequence":"additional","affiliation":[{"name":"A.A. Smorodintsev Department of Virology and Immunology, Institute of Experimental Medicine (IEM), 12 Acad. Pavlov Str., 197022 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2253-6263","authenticated-orcid":false,"given":"Nina B.","family":"Brutch","sequence":"additional","affiliation":[{"name":"Department of Oil and Fiber Crops, N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 42\u201344 B. Morskaya Str., 190000 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ebert, A.W., and Engels, J.M.M. (2020). Plant Biodiversity and Genetic Resources Matter!. Plants, 9.","DOI":"10.3390\/plants9121706"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s40066-021-00318-5","article-title":"Impact of Climate Change on Biodiversity and Food Security: A Global Perspective","volume":"10","author":"Muluneh","year":"2021","journal-title":"Agric. Food Secur."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Trusiak, M., Plitta-Michalak, B.P., and Michalak, M. (2022). Choosing the Right Path for the Successful Storage of Seeds. 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