{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:13:14Z","timestamp":1791994394076,"version":"4.4.0"},"reference-count":68,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Biodiversity Future Center\u2013NBFC","award":["CN00000033"],"award-info":[{"award-number":["CN00000033"]}]},{"name":"National Biodiversity Future Center\u2013NBFC","award":["CUP B73C22000790001"],"award-info":[{"award-number":["CUP B73C22000790001"]}]},{"name":"Fondo per il Programma Nazionale di Ricerca e Progetti di Rilevante Interesse Nazionale","award":["P2022CKMPW_002"],"award-info":[{"award-number":["P2022CKMPW_002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Helichrysum Mill. is one of the largest genera in the Asteraceae family, and in Madagascar, a plant paradise with ca 14,000 species, 111 Helichrysum species are known, 110 of which are endemic. In this work, the essential oil of endemic Helichrysum manopappoides Humbert, obtained by steam distillation, was investigated chemically and biologically. The spectrometric data obtained by GC-MS (Gas Chromatography\u2013Mass Spectrometry) analysis highlighted the presence of three major compounds, such as eucalyptol (7.38 \u00b1 0.36%), \u03b1-humulene (14.75 \u00b1 0.79%), and \u03b2-caryophyllene (19.78 \u00b1 0.89%), which were also structurally confirmed by NMR (Nuclear Magnetic Resonance) spectroscopic investigation. Biological results showed potential antimicrobial, antioxidant, and antibiofilm effects of both the H manopappoides essential oil and the main components identified by GC-MS, enhancing an interesting approach for intestinal infections, being active against Escherichia coli, Listeria monocytogenes, Shigella sonnei, and Salmonella enterica ser. typhimurium strains.<\/jats:p>","DOI":"10.3390\/plants15050672","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T12:19:23Z","timestamp":1771935563000},"page":"672","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phytochemistry, Antimicrobial, and Antibiofilm Properties of Malagasy Helichrysum manopappoides Essential Oil"],"prefix":"10.3390","volume":"15","author":[{"given":"Giusy","family":"Castagliuolo","sequence":"first","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Sordillo","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henintsoa Jean Baptiste","family":"Ramaminirina","sequence":"additional","affiliation":[{"name":"Department of Environment, Institut Universitaire de l\u2019Innovation Technologique, University of Vakinankaratra, P.O. Box 180, Antsirabe 110, Madagascar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Di Girolamo","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Varcamonti","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7961-3894","authenticated-orcid":false,"given":"Natale","family":"Badalamenti","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), Universit\u00e0 degli Studi di Palermo, Viale delle Scienze, Ed. 17, 90128 Palermo, Italy"},{"name":"National Biodiversity Future Center (NBFC), 90133 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2950-2739","authenticated-orcid":false,"given":"Stephan Richard","family":"Rakotonandrasana","sequence":"additional","affiliation":[{"name":"Department of Ethnobotany and Botany, National Center of Applied Pharmaceutical Research, P.O. Box 702, Antananarivo 101, Madagascar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6689-8365","authenticated-orcid":false,"given":"Anna","family":"Zanfardino","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0583-0487","authenticated-orcid":false,"given":"Maurizio","family":"Bruno","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), Universit\u00e0 degli Studi di Palermo, Viale delle Scienze, Ed. 17, 90128 Palermo, Italy"},{"name":"National Biodiversity Future Center (NBFC), 90133 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent Emile","family":"Rasamison","sequence":"additional","affiliation":[{"name":"Department of Environment, Institut Universitaire de l\u2019Innovation Technologique, University of Vakinankaratra, P.O. Box 180, Antsirabe 110, Madagascar"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"ref_1","unstructured":"(2025, November 24). POWO, Plants of the World Online. Available online: https:\/\/powo.science.kew.org\/taxon\/urn:lsid:ipni.org:names:331648-2."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"608","DOI":"10.12705\/633.8","article-title":"Phylogenetic relationships in Helichrysum (Compositae: Gnaphalieae) and related genera: Incongruence between nuclear and plastid phylogenies, biogeographic and morphological patterns, and implications for generic delimitation","volume":"63","author":"Unwin","year":"2014","journal-title":"Taxonomy"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Glumac, M., Ja\u017eo, Z., Pa\u0161tar, V., Golemac, A., \u010cike\u0161 \u010culi\u0107, V., Bekti\u0107, S., Radan, M., and Carev, I. (2023). 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