{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:11:07Z","timestamp":1790709067422,"version":"4.1.0"},"reference-count":42,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006533","name":"Ministry of Agriculture of the Czech Republic","doi-asserted-by":"crossref","award":["RO1525"],"award-info":[{"award-number":["RO1525"]}],"id":[{"id":"10.13039\/501100006533","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007403","name":"National Agency for Agricultural Research","doi-asserted-by":"crossref","award":["QK21010200"],"award-info":[{"award-number":["QK21010200"]}],"id":[{"id":"10.13039\/501100007403","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Genetic and climatic factors influence the nutritional content of fruit, with vitamin C being a key component. Using HPLC, we quantified the amount of vitamin C in cherries, apricots, plums, and apples from 2022 to 2024. Contents ranged from 1.6 to 24.6 mg\/100 g fresh weight basis (FW), with apples and plums displaying the highest coefficient of variation (32.53% and 45.25%). The highest content was consistently found in accession \u2018HL827\u2019, which exceeded 20 mg\/100 g FW. Cherries reached up to 12.1 mg\/100 g FW in 2023 (\u201813590\u2032), but decreased to 1.9 mg\/100 g FW in 2024 (\u2018Jacinta\u2019). Apricots showed high fluctuation, with \u2018Betinka\u2019, \u2018Candela\u2019, and \u2018HL08-052\u2019 exceeding the 30% variance coefficient. Accessions that remained stable (\u2018HL96-266\u2019) maintained a low variance only. Plums were the most sensitive, experiencing low vitamin C content under hot and dry conditions. Regression analysis identified temperature (NTavg-20) as the dominant climatic driver in plums and cherries (R2 = 0.999, p &lt; 0.05 and R2 = 0.995, p &lt; 0.05) respectively, whereas apples and apricots showed negligible responses (R2 \u2264 0.210). These findings underscore the importance of genotype\/environment interactions at the local level and highlight the value of stable accessions as valuable resources for breeding cultivars with high and resilient vitamin C content.<\/jats:p>","DOI":"10.3390\/plants15050793","type":"journal-article","created":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T16:17:31Z","timestamp":1772641051000},"page":"793","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Climatic Conditions and Genotypes on Vitamin C Levels in Stone Species and Apple Cultivars"],"prefix":"10.3390","volume":"15","author":[{"given":"Aneta","family":"B\u00edlkov\u00e1","sequence":"first","affiliation":[{"name":"Department of Laboratory Complement, Research and Breeding Institute of Pomology Holovousy Ltd., 50801 Holovousy, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7345-5717","authenticated-orcid":false,"given":"Pavol","family":"Suran","sequence":"additional","affiliation":[{"name":"Department of Genetics and Breeding, Research and Breeding Institute of Pomology Holovousy Ltd., 50801 Holovousy, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e1\u0161a","family":"Jirou\u0161ov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Laboratory Complement, Research and Breeding Institute of Pomology Holovousy Ltd., 50801 Holovousy, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"\u0160epsov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Laboratory Complement, Research and Breeding Institute of Pomology Holovousy Ltd., 50801 Holovousy, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1335-496X","authenticated-orcid":false,"given":"Martin","family":"M\u00e9sz\u00e1ros","sequence":"additional","affiliation":[{"name":"Department of Technology, Research and Breeding Institute of Pomology Holovousy Ltd., 50801 Holovousy, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mieszczakowska-Fr\u0105c, M., Celejewska, K., and P\u0142ocharski, W. (2021). Impact of innovative technologies on the content of vitamin C and its bioavailability from processed fruit and vegetable products. Antioxidants, 10.","DOI":"10.3390\/antiox10010054"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1038\/30728","article-title":"The biosynthetic pathway of vitamin C in higher plants","volume":"393","author":"Wheeler","year":"1998","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1002\/(SICI)1097-0010(20000515)80:7<825::AID-JSFA598>3.0.CO;2-6","article-title":"Plant L-ascorbic acid: Chemistry, function, metabolism, bioavailability and effects of processing","volume":"80","author":"Davey","year":"2000","journal-title":"J. Sci. Food Agric."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jfca.2015.09.008","article-title":"Stability of vitamin C in fruit and vegetable homogenates stored at different temperatures","volume":"45","author":"Phillips","year":"2016","journal-title":"J. Food Compos. Anal."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.freeradbiomed.2018.03.033","article-title":"Ascorbic acid metabolism and functions: A comparison of plants and mammals","volume":"122","author":"Smirnoff","year":"2018","journal-title":"Free Radic. Biol. Med."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Mellidou, I., Keulemans, J., Kanellis, A.K., and Davey, M.V. (2012). Regulation of fruit ascorbic acid concentrations during ripening in high and low vitamin C tomato cultivars. BMC Plant Biol., 12.","DOI":"10.1186\/1471-2229-12-239"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1111\/j.1365-2621.1982.tb12750.x","article-title":"Ascorbic acid and vitamin A activity in selected vegetables from different geographical areas of the United States","volume":"47","author":"Klein","year":"1982","journal-title":"J. Food Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0925-5214(00)00133-2","article-title":"Preharvest and postharvest factors influencing vitamin C content of horticultural crops","volume":"20","author":"Lee","year":"2000","journal-title":"Postharvest Biol. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"696","DOI":"10.21273\/HORTSCI.48.6.696","article-title":"Vitamins, flavonoids, and phenolic acid levels in early- and late-ripening apricot (Prunus armeniaca L.) cultivars from Turkey","volume":"48","author":"Kan","year":"2013","journal-title":"HortScience"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.foodchem.2007.08.004","article-title":"Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.)","volume":"107","author":"Usenik","year":"2008","journal-title":"Food Chem."},{"key":"ref_11","first-page":"1","article-title":"The relationship between fruit color and fruit quality of sweet cherry (Prunus avium L. cv. \u20180900 Ziraat\u2019)","volume":"1","author":"Karakaya","year":"2019","journal-title":"Turk. J. Food Agric. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1002\/jsfa.8106","article-title":"Characterization of autochthonous sweet cherry cultivars (Prunus avium L.) of southern Italy for fruit quality, bioactive compounds and antioxidant activity","volume":"97","author":"Russo","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.scienta.2011.05.040","article-title":"Physico-chemical characteristics of apricot (Prunus armeniaca L.) grown in northern areas of Pakistan","volume":"130","author":"Ali","year":"2011","journal-title":"Sci. Hortic."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"37","DOI":"10.24326\/asphc.2023.4807","article-title":"Determination of optimum harvest time and physical and chemical quality properties of Shalakh (Aprikoz) apricot cultivar during fruit ripening","volume":"22","author":"Tan","year":"2023","journal-title":"Acta Sci. Pol. Hortorum Cultus"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1021\/jf0344690","article-title":"Nutrients and Antioxidant Molecules in Yellow Plums (Prunus domestica L.) from Conventional and Organic Productions: A Comparative Study","volume":"52","author":"Lucarini","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2820","DOI":"10.1021\/jf970032e","article-title":"Research on Chemical Composition of Some Varieties of European Plums (Prunus domestica) Adapted to the Aegean District of Turkey","volume":"45","author":"Nergiz","year":"1997","journal-title":"J. Agric. Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1051\/fruits\/2013102","article-title":"Wild blackthorn (Prunus spinosa L.) and hawthorn (Crataegus monogyna Jacq.) fruits as valuable sources of antioxidants","volume":"69","author":"Morales","year":"2014","journal-title":"Fruits"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Kuru Berk, S., Tas, A., Orman, E., Gundogdu, M., Necas, T., Ondrasek, I., Karatas, N., and Ercisli, S. (2020). Agro morphological and biochemical characterization of 23 plum genotypes: Genotyping diversity of vitamin C in plum. Agronomy, 10.","DOI":"10.3390\/agronomy10111748"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"S2626","DOI":"10.1080\/10942912.2017.1381705","article-title":"Ascorbic acid content in apple pulp, peel, and monovarietal cloudy juices of 64 different cultivars","volume":"20","author":"Bassi","year":"2017","journal-title":"Int. J. Food Prop."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.foodchem.2017.01.014","article-title":"Variation of ascorbic acid concentration in fruits of cultivated and wild apples","volume":"225","author":"Fang","year":"2017","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2729","DOI":"10.1007\/s13197-018-3195-x","article-title":"Nutritional characterization of apple as a function of genotype","volume":"55","author":"Kumar","year":"2018","journal-title":"J. Food Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Fenech, M., Amaya, I., Valpuesta, V., and Botella, M.A. (2019). Vitamin C content in fruits: Biosynthesis and regulation. Front. Plant Sci., 9.","DOI":"10.3389\/fpls.2018.02006"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.14720\/aas.2022.118.2.2404","article-title":"Studies of the impact of environmental conditions and varietal features of sweet cherry on the accumulation of vitamin C in fruits by using the regression analysis method","volume":"118","author":"Ivanova","year":"2022","journal-title":"Acta Agric. Slov."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2604","DOI":"10.1093\/jxb\/erad505","article-title":"The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions","volume":"75","author":"Smirnoff","year":"2024","journal-title":"J. Exp. Bot."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Terzaghi, M., and De Tullio, M.C. (2022). The perils of planning strategies to increase vitamin C content in plants: Beyond the hype. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1096549"},{"key":"ref_26","first-page":"45","article-title":"Genetic diversity in fruit nutritional composition, anthocyanins, phenolics, and antioxidant capacity of plum (Prunus domestica) genotypes","volume":"14","author":"Nisar","year":"2015","journal-title":"Acta Sci. Pol. Hortorum Cultus"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"17105","DOI":"10.1038\/nmicrobiol.2017.105","article-title":"The importance of anabolism in microbial control over soil carbon storage","volume":"2","author":"Liang","year":"2017","journal-title":"Nat. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1093\/plphys\/kiad241","article-title":"L-ascorbic acid metabolism and regulation in fruit crops","volume":"192","author":"Liao","year":"2023","journal-title":"Plant Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3143","DOI":"10.53350\/pjmhs2115123143","article-title":"Antioxidative role of synthetic and natural ascorbic acid (vitamin C) in metabolic pathways of a biological system","volume":"15","author":"Shuja","year":"2021","journal-title":"Pak. J. Med. Health Sci."},{"key":"ref_30","first-page":"595","article-title":"Determination of organics, phenolics, sugars and vitamin C contents of some cherry cultivars (Prunus avium)","volume":"14","author":"Bilge","year":"2012","journal-title":"Int. J. Agric. Biol."},{"key":"ref_31","first-page":"928","article-title":"Some phytochemical characteristics of cherry cultivars and relations between these characteristics","volume":"8","year":"2021","journal-title":"Turk. J. Agric. Nat. Sci."},{"key":"ref_32","first-page":"C722","article-title":"Antioxidant and antiradical capacities in apricot (Prunus armeniaca L.) fruits: Variations from genotypes, years, and analytical methods","volume":"75","author":"Engel","year":"2010","journal-title":"J. Food Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"266","DOI":"10.4067\/S0718-58392018000200266","article-title":"Chemical composition and antioxidant activity of some apricot varieties at different ripening stages","volume":"78","author":"Iordanescu","year":"2018","journal-title":"Chil. J. Agric. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.plantsci.2008.03.008","article-title":"Distribution and metabolism of ascorbic acid in apple fruits (Malus domestica Borkh. cv. Gala)","volume":"174","author":"Li","year":"2008","journal-title":"Plant Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1051\/fruits\/2015042","article-title":"Chemometric characterization of peach, nectarine and plum cultivars according to fruit phenolic content and antioxidant activity","volume":"71","author":"Mitic","year":"2016","journal-title":"Fruits"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Drogoudi, P., and Pantelidis, G. (2022). Phenotypic variation and peel contribution to fruit antioxidant contents in European and Japanese plums. Plants, 11.","DOI":"10.3390\/plants11101338"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1080\/10942912.2020.1820515","article-title":"L-ascorbic acid and \u03b1-tocopherol content in apple pulp: A comparison between 24 cultivars and annual variations during three harvest seasons","volume":"23","author":"Bianchi","year":"2020","journal-title":"Int. J. Food Prop."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Li, Y., Sun, H., Li, J., Qin, S., Yang, W., Ma, X., Qiao, X., and Yang, B. (2021). Effects of genetic background and altitude on sugars, malic acid and ascorbic acid in fruits of wild and cultivated apples (Malus sp.). Foods, 10.","DOI":"10.3390\/foods10122950"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1002\/biot.200700084","article-title":"Biotechnology of flavonoids and other phenylpropanoid-derived natural products. Part I: Chemical diversity, impacts on plant biology and human health","volume":"2","author":"Ververidis","year":"2007","journal-title":"Biotechnol. J."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"10795","DOI":"10.1021\/jf072035p","article-title":"Apple peel as a contributor to whole fruit quantity of potentially healthful bioactive compounds: Cultivar and year implication","volume":"55","author":"Lata","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_41","first-page":"985","article-title":"Impact of high temperature stress on ascorbic acid concentrtion in tomato","volume":"1194","author":"Fenoll","year":"2018","journal-title":"Acta Hortic."},{"key":"ref_42","unstructured":"UKZUZ (2019). Determination of Vitamin C Content by HPLC Method: Unified Working Procedure No. 10260.1, Central Institute for Supervising and Testing in Agriculture."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/5\/793\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T05:16:28Z","timestamp":1772774188000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/5\/793"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,4]]},"references-count":42,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["plants15050793"],"URL":"https:\/\/doi.org\/10.3390\/plants15050793","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,4]]}}}