{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:12:36Z","timestamp":1790820756507,"version":"4.1.0"},"reference-count":50,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jiangxi Forestry Administration","award":["JXYCZX\u30142023\u3015131"],"award-info":[{"award-number":["JXYCZX\u30142023\u3015131"]}]},{"DOI":"10.13039\/501100010857","name":"Jiangxi Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["20252BCF320010, 20252BAC200410, 20242BAB20305, 20232BAB215045, 20244BCE52294, 20244BCE52287"],"award-info":[{"award-number":["20252BCF320010, 20252BAC200410, 20242BAB20305, 20232BAB215045, 20244BCE52294, 20244BCE52287"]}],"id":[{"id":"10.13039\/501100010857","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Jiangxi Academy of Forestry","award":["2025521002, 2025521001, 2024521001"],"award-info":[{"award-number":["2025521002, 2025521001, 2024521001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The provincial-level registered superior cultivar Camellia chekiangoleosa \u2018Ganhongyou 1\u2019 boasts superior economic traits coupled with significant ornamental value, driving demand for an efficient propagation system. Consequently, this study aimed to develop a rapid micropropagation protocol by investigating culture conditions using semi-woody nodal segments with axillary buds as explants on Hyponex basal medium supplemented with varying combinations of plant growth regulators. Contamination was effectively minimized to 18% by a combined approach of surface sterilization (75% ethanol, 0.1% HgCl2, and 20% NaClO) and incorporating 1 mL\/L bactericide into the induction medium. For bud induction, the optimal medium was 2 g\/L Hyponex supplemented with 1.0 mg\/L 6-benzylaminopurine (6-BA) and 0.2 mg\/L indole-3-acetic acid (IAA), achieving an 86.67% induction rate. The best proliferation was achieved on the medium containing 2 g\/L Hyponex, 1.0 mg\/L 6-BA, 0.15 mg\/L 3-indolebutyric acid (IBA), and 0.5 mg\/L gibberellic acid (GA3), yielding a proliferation coefficient of 6.53. A combined strategy, integrating in vitro pre-culture with ex vitro treatment, proved most effective for rooting and acclimatization: shoots were first pre-cultured for 20 days on 1\/2 strength Hyponex medium supplemented with 0.5 mg\/L 1-naphthaleneacetic acid (NAA) and 2.0 mg\/L IBA, followed by ex vitro base treatment with 1.0 g\/L ABT (a rooting powder complex) solution before transplantation into seedling bags. This approach resulted in an 88% survival rate. Furthermore, anatomical analysis revealed the origin of adventitious root primordia from phloem parenchyma cells, thereby confirming a phloem-rooting pattern for this species. In conclusion, this study establishes a practical and efficient micropropagation protocol for \u2018Ganhongyou 1\u2019, providing a reliable technical foundation for its commercial-scale seedling production.<\/jats:p>","DOI":"10.3390\/plants15060871","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T15:32:09Z","timestamp":1773243129000},"page":"871","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["An Efficient Micropropagation Protocol for Camellia chekiangoleosa \u2018Ganhongyou 1\u2019 via Stem Segment Culture"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2502-9415","authenticated-orcid":false,"given":"Anni","family":"Liu","sequence":"first","affiliation":[{"name":"College of Forestry, Jiangxi Agricultural University, Nanchang 330108, China"},{"name":"Jiangxi Provincial Key Laboratory of Oil-Tea Camellia Resource Cultivation and Utilization, Jiangxi Academy of Forestry, Nanchang 330032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixuan","family":"Peng","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oil-Tea Camellia Resource Cultivation and Utilization, Jiangxi Academy of Forestry, Nanchang 330032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Forestry, Jiangxi Agricultural University, Nanchang 330108, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiangqiang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oil-Tea Camellia Resource Cultivation and Utilization, Jiangxi Academy of Forestry, Nanchang 330032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Zha","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oil-Tea Camellia Resource Cultivation and Utilization, Jiangxi Academy of Forestry, Nanchang 330032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Wen","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oil-Tea Camellia Resource Cultivation and Utilization, Jiangxi Academy of Forestry, Nanchang 330032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"330","DOI":"10.21273\/JASHS04164-17","article-title":"Characteristics of Developmental Differences between Fertile and Aborted Ovules in Camellia oleifera","volume":"142","author":"Gao","year":"2017","journal-title":"J. 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