{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T05:49:07Z","timestamp":1774936147404,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T00:00:00Z","timestamp":1774742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program under the Marie Sk\u0142odowska-Curie","award":["101007438"],"award-info":[{"award-number":["101007438"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program under the Marie Sk\u0142odowska-Curie","award":["872417"],"award-info":[{"award-number":["872417"]}]},{"DOI":"10.13039\/501100002923","name":"CONICET","doi-asserted-by":"crossref","award":["PIP-1220200101905CO"],"award-info":[{"award-number":["PIP-1220200101905CO"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003074","name":"ANPCyT\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t              https:\/\/ror.org\/03stxzb56","doi-asserted-by":"publisher","award":["PICT-2021-I-A-00576"],"award-info":[{"award-number":["PICT-2021-I-A-00576"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad Nacional del Sur\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t              https:\/\/ror.org\/028crwz56","award":["PGI UNS 24\/A261"],"award-info":[{"award-number":["PGI UNS 24\/A261"]}]},{"name":"CUIA\/CONICET","award":["2019\u201424120190100015CO"],"award-info":[{"award-number":["2019\u201424120190100015CO"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Eragrostis curvula serves as a valuable model for studying diplosporous apomixis due to its unique reproductive mode, wide ploidy range, and extensive genomic resources. A major limitation for reproductive studies in this species is the difficulty of isolating female tissues at precise developmental stages, for example, for transcriptomics studies, since different floral tissues can introduce expression noise from non-target tissues. To overcome this, we performed a detailed cytoembryological and morphometric characterization of male and female development in seven E. curvula genotypes with different ploidy levels (2X\u20137X) and reproductive modes (sexual, facultative apomictic, and obligate apomictic). Using differential interference contrast microscopy and methyl salicylate clarification, we described key cytological stages of male and female development. These stages were then correlated with external floral parameters, including pistil, ovary, style, and anther length, to generate genotype-specific developmental calendars. Pistil length showed the strongest association with female developmental stage, particularly during the early phases of ovule development, enabling more precise staging. Synchrony between male and female development was also evaluated, revealing no consistent differences among reproductive modes or ploidy levels. This genotype-informed framework provides a practical tool for stage prediction and tissue selection, supporting future reproductive, developmental, and comparative studies in E. curvula and related grasses.<\/jats:p>","DOI":"10.3390\/plants15071050","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T09:57:11Z","timestamp":1774864631000},"page":"1050","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Precise Reproductive Calendar of Sexual and Apomictic Genotypes of Eragrostis curvula"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8331-6865","authenticated-orcid":false,"given":"Juan Pablo","family":"Selva","sequence":"first","affiliation":[{"name":"Centro de Recursos Naturales Renovables de la Zona Semi\u00e1rida (CERZOS-CONICET-UNS), CCT CONICET Bah\u00eda Blanca, Bah\u00eda Blanca 8000, Argentina"},{"name":"Departamento de Agronom\u00eda, Universidad Nacional del Sur (UNS), Bah\u00eda Blanca 8000, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0520-1296","authenticated-orcid":false,"given":"Diego","family":"Zappacosta","sequence":"additional","affiliation":[{"name":"Centro de Recursos Naturales Renovables de la Zona Semi\u00e1rida (CERZOS-CONICET-UNS), CCT CONICET Bah\u00eda Blanca, Bah\u00eda Blanca 8000, Argentina"},{"name":"Departamento de Agronom\u00eda, Universidad Nacional del Sur (UNS), Bah\u00eda Blanca 8000, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9339-1802","authenticated-orcid":false,"given":"Cristian Andr\u00e9s","family":"Gallo","sequence":"additional","affiliation":[{"name":"Centro de Recursos Naturales Renovables de la Zona Semi\u00e1rida (CERZOS-CONICET-UNS), CCT CONICET Bah\u00eda Blanca, Bah\u00eda Blanca 8000, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petrus","family":"Bisp","sequence":"additional","affiliation":[{"name":"Centro de Recursos Naturales Renovables de la Zona Semi\u00e1rida (CERZOS-CONICET-UNS), CCT CONICET Bah\u00eda Blanca, Bah\u00eda Blanca 8000, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2585-5649","authenticated-orcid":false,"given":"Emidio","family":"Albertini","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Universit\u00e0 degli Studi di Perugia, 06121 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3755-9062","authenticated-orcid":false,"given":"Viviana","family":"Echenique","sequence":"additional","affiliation":[{"name":"Centro de Recursos Naturales Renovables de la Zona Semi\u00e1rida (CERZOS-CONICET-UNS), CCT CONICET Bah\u00eda Blanca, Bah\u00eda Blanca 8000, Argentina"},{"name":"Departamento de Agronom\u00eda, Universidad Nacional del Sur (UNS), Bah\u00eda Blanca 8000, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/j.1537-2197.1958.tb13125.x","article-title":"Apomixis in the Gramineae: Panicoideae","volume":"45","author":"Brown","year":"1958","journal-title":"Am. 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