{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T10:58:35Z","timestamp":1774868315258,"version":"3.50.1"},"reference-count":56,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T00:00:00Z","timestamp":1774742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Mobilizing Agenda TEC4GREEN","award":["C05-i01.01\u20142022.PC644948561-00000052"],"award-info":[{"award-number":["C05-i01.01\u20142022.PC644948561-00000052"]}]},{"name":"iNOVA4Health\u2014Programme in Translational Medicine"},{"name":"Associated Laboratory LS4FUTURE","award":["LA\/P\/0087\/2020"],"award-info":[{"award-number":["LA\/P\/0087\/2020"]}]},{"name":"R&D Unit GREEN-IT\u2014Bioresources for Sustainability","award":["UID\/04551\/2025"],"award-info":[{"award-number":["UID\/04551\/2025"]}]},{"name":"R&D Unit GREEN-IT\u2014Bioresources for Sustainability","award":["UID\/PRR\/04551\/2025"],"award-info":[{"award-number":["UID\/PRR\/04551\/2025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Botrytis cinerea, the causative agent of gray mold, is a major fungal pathogen affecting a wide range of economically important crops. To identify sustainable alternatives to chemical fungicides, this study characterized the biocontrol potential of two bacterial strains, Serratia quinivorans NFX21 and Pseudomonas thivervalensis NFX104, through genomic analysis and functional assays targeting key stages of fungal growth and plant infection. The NFX21 and NFX104 strains significantly inhibited B. cinerea mycelial growth (~35%) and strongly suppressed conidial germination with performances comparable to the reference biocontrol strain Bacillus amyloliquefaciens QST 713. In tomato detached-leaf and whole-plant pot assays, application of NFX21 and NFX104 significantly reduced gray mold incidence and lesion severity relative to nontreated infected plants (53\u201364%, detached leaves; 12\u201313%, whole-plant assays), achieving disease control levels similar to those obtained with the commercial biofungicide Serenade ASO\u00ae. Whole-genome sequencing allowed the taxonomic assignment of the NFX strains and revealed a rich repertoire of biosynthetic gene clusters and antifungal determinants. The NFX21 genome contained genes associated with N-acyl-homoserine lactone-mediated quorum-sensing and production of lipopeptides, siderophores, and extracellular lytic enzymes. The NFX104 genome harbored clusters involved in the biosynthesis of multiple siderophores, 2,4-diacetylphloroglucinol and hydrogen cyanide. Moreover, both the NFX21 and NFX104 genomes contained additional low-homology clusters that potentially encode for novel unexplored metabolites. Collectively, these results support the translational potential of NFX21 and NFX104 as biocontrol candidates for sustainable, integrated management of gray mold caused by B. cinerea.<\/jats:p>","DOI":"10.3390\/plants15071052","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T09:57:11Z","timestamp":1774864631000},"page":"1052","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional and Genomic Characterization of Serratia quinivorans NFX21 and Pseudomonas thivervalensis NFX104, Novel Biocontrol Agents Against Botrytis cinerea"],"prefix":"10.3390","volume":"15","author":[{"given":"Sara","family":"Tedesco","sequence":"first","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filipa T.","family":"Silva","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarida","family":"Pimenta","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2948-6230","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"Bustos Gaspar","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8228-0576","authenticated-orcid":false,"given":"Marta","family":"Nunes da Silva","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4169-005 Porto, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5605-1419","authenticated-orcid":false,"given":"Maria Teresa","family":"Barreto Crespo","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1184-3794","authenticated-orcid":false,"given":"Francisco X.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"iBET\u2014Instituto de Biologia Experimental e Tecnol\u00f3gica, 2780-157 Oeiras, Portugal"},{"name":"ITQB NOVA\u2014Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.cropro.2016.08.014","article-title":"Interface of the Environment and Occurrence of Botrytis cinerea in Pre-Symptomatic Tomato Crops","volume":"90","author":"Wakeham","year":"2016","journal-title":"Crop Prot."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e70074","DOI":"10.1111\/1751-7915.70074","article-title":"Understanding Efflux-Mediated Multidrug Resistance in Botrytis cinerea for Improved Management of Fungicide Resistance","volume":"18","author":"Wu","year":"2025","journal-title":"Microb. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Roca-Couso, R., Flores-F\u00e9lix, J.D., and Rivas, R. (2021). Mechanisms of Action of Microbial Biocontrol Agents against Botrytis cinerea. J. Fungi, 7.","DOI":"10.3390\/jof7121045"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Leroux, P. (2007). Chemical Control of Botrytis and Its Resistance to Chemical Fungicides. Botrytis: Biology, Pathology and Control, Springer Netherlands.","DOI":"10.1007\/978-1-4020-2626-3_12"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1080\/09583157.2018.1548574","article-title":"Biofungicides as Alternative to Synthetic Fungicide Control of Grey Mould (Botrytis cinerea)\u2014Prospects and Challenges","volume":"29","author":"Abbey","year":"2019","journal-title":"Biocontrol Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Sofianos, G., Samaras, A., and Karaoglanidis, G. (2023). Multiple and Multidrug Resistance in Botrytis cinerea: Molecular Mechanisms of MLR\/MDR Strains in Greece and Effects of Co-Existence of Different Resistance Mechanisms on Fungicide Sensitivity. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1273193"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Bonaterra, A., Badosa, E., Daranas, N., Franc\u00e9s, J., Rosell\u00f3, G., and Montesinos, E. (2022). Bacteria as Biological Control Agents of Plant Diseases. Microorganisms, 10.","DOI":"10.3390\/microorganisms10091759"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"He, D.C., He, M.H., Amalin, D.M., Liu, W., Alvindia, D.G., and Zhan, J. (2021). Biological Control of Plant Diseases: An Evolutionary and Eco-Economic Consideration. Pathogens, 10.","DOI":"10.3390\/pathogens10101311"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1111\/ppa.13555","article-title":"Biological Control of Plant Diseases\u2014What Has Been Achieved and What Is the Direction?","volume":"71","author":"Collinge","year":"2022","journal-title":"Plant Pathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"fiw249","DOI":"10.1093\/femsec\/fiw249","article-title":"The Contribution of Genome Mining Strategies to the Understanding of Active Principles of PGPR Strains","volume":"93","author":"Paterson","year":"2017","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"K\u00f6hl, J., Kolnaar, R., and Ravensberg, W.J. (2019). Mode of Action of Microbial Biological Control Agents against Plant Diseases: Relevance beyond Efficacy. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.00845"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"105780","DOI":"10.1016\/j.biocontrol.2025.105780","article-title":"Biocontrol Activity of Novel and Known Bioproducts Based on Bacillus Strains and Basic Products against Botrytis cinerea in Tomato Fruit","volume":"206","author":"Panebianco","year":"2025","journal-title":"Biol. Control."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ajijah, N., Fiodor, A., Dziurzynski, M., Stasiuk, R., Pawlowska, J., Dziewit, L., and Pranaw, K. (2023). Biocontrol Potential of Pseudomonas Protegens ML15 against Botrytis cinerea Causing Gray Mold on Postharvest Tomato (Solanum lycopersicum Var. Cerasiforme). Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1288408"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4080","DOI":"10.1111\/1462-2920.13845","article-title":"Identification and Occurrence of the Hydroxamate Siderophores Aerobactin, Putrebactin, Avaroferrin and Ochrobactin C as Virulence Factors from Entomopathogenic Bacteria","volume":"19","author":"Hirschmann","year":"2017","journal-title":"Environ. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"100802","DOI":"10.1016\/j.margen.2020.100802","article-title":"Complete Genome Sequence of a Marine-Derived Bacterium Pseudomonas Sp. SXM-1 and Characterization of Its Siderophore through AntiSMASH Analysis and with Mass Spectroscopic Method","volume":"55","author":"Guo","year":"2021","journal-title":"Mar. Genomics"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1128\/JB.01639-12","article-title":"Ferric-Pyoverdine Recognition by Fpv Outer Membrane Proteins of Pseudomonas Protegens Pf-5","volume":"195","author":"Hartney","year":"2013","journal-title":"J. Bacteriol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/s10534-016-9929-1","article-title":"Pyoverdine and Histicorrugatin-Mediated Iron Acquisition in Pseudomonas thivervalensis","volume":"29","author":"Matthijs","year":"2016","journal-title":"BioMetals"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1128\/aem.62.2.552-563.1996","article-title":"Conservation of the 2,4-Diacetylphloroglucinol Biosynthesis Locus among Fluorescent Pseudomonas Strains from Diverse Geographic Locations","volume":"62","author":"Keel","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Redondo-Nieto, M., Barret, M., Morrissey, J., Germaine, K., Mart\u00ednez-Granero, F., Barahona, E., Navazo, A., S\u00e1nchez-Contreras, M., Moynihan, J.A., and Muriel, C. (2013). Genome Sequence Reveals That Pseudomonas fluorescens F113 Possesses a Large and Diverse Array of Systems for Rhizosphere Function and Host Interaction. BMC Genomics, 14.","DOI":"10.1186\/1471-2164-14-54"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1094\/MPMI.2003.16.6.525","article-title":"Phylogeny of HCN Synthase-Encoding HcnBC Genes in Biocontrol Fluorescent Pseudomonads and Its Relationship with Host Plant Species and HCN Synthesis Ability","volume":"16","author":"Ramette","year":"2003","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Elad, Y., Vivier, M., and Fillinger, S. (2016). Botrytis, the Good, the Bad and the Ugly. Botrytis\u2014The Fungus, the Pathogen and Its Management in Agricultural Systems, Springer International Publishing.","DOI":"10.1007\/978-3-319-23371-0_1"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5265","DOI":"10.1111\/1462-2920.13568","article-title":"Non-specific Transient Mutualism between the Plant Parasitic Nematode, Bursaphelenchus xylophilus, and the Opportunistic Bacterium Serratia quinivorans BXF1, a Plant-growth Promoting Pine Endophyte with Antagonistic Effects","volume":"18","author":"Nascimento","year":"2016","journal-title":"Environ. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0038-0717(02)00283-3","article-title":"Soil-Borne Strain IC14 of Serratia plymuthica with Multiple Mechanisms of Antifungal Activity Provides Biocontrol of Botrytis cinerea and Sclerotinia sclerotiorum Diseases","volume":"35","author":"Kamensky","year":"2003","journal-title":"Soil Biol. Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Chlebek, D., Grebtsova, V., Pi\u0144ski, A., \u017bur-Pi\u0144ska, J., and Hupert-Kocurek, K. (2022). Genetic Determinants of Antagonistic Interactions and the Response of New Endophytic Strain Serratia quinivorans KP32 to Fungal Phytopathogens. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232415561"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Nascimento, F.X., Ur\u00f3n, P., Glick, B.R., Giachini, A., and Rossi, M.J. (2021). Genomic Analysis of the 1-Aminocyclopropane-1-Carboxylate Deaminase-Producing Pseudomonas thivervalensis SC5 Reveals Its Multifaceted Roles in Soil and in Beneficial Interactions with Plants. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.752288"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"106095","DOI":"10.1016\/j.pestbp.2024.106095","article-title":"Pseudomonas thivervalensis K321, a Promising and Effective Biocontrol Agent for Managing Apple Valsa Canker Triggered by Valsa Mali","volume":"204","author":"Meng","year":"2024","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"105514","DOI":"10.1016\/j.biocontrol.2024.105514","article-title":"The Novel Pseudomonas thivervalensis Strain JI6 Promotes Growth and Controls Rusty Root Rot Disease in Panax ginseng","volume":"193","author":"Liu","year":"2024","journal-title":"Biol. Control."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s10658-008-9411-1","article-title":"Induction of Systemic Resistance, Root Colonisation and Biocontrol Activities of the Rhizospheric Strain of Serratia plymuthica Are Dependent on N-Acyl Homoserine Lactones","volume":"124","author":"Pang","year":"2008","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/j.1365-2958.1996.tb02495.x","article-title":"Involvement of N-acyl-L-homoserine Lactone Autoinducers in Controlling the Multicellular Behaviour of Serratia liquefaciens","volume":"20","author":"Eberl","year":"1996","journal-title":"Mol. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1111\/j.1574-6968.2007.00681.x","article-title":"Quorum-Sensing Signaling Is Required for Production of the Antibiotic Pyrrolnitrin in a Rhizospheric Biocontrol Strain of Serratia plymuthica","volume":"270","author":"Liu","year":"2007","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Liu, X., Jia, J., Popat, R., Ortori, C.A., Li, J., Diggle, S.P., Gao, K., and C\u00e1mara, M. (2011). Characterisation of Two Quorum Sensing Systems in the Endophytic Serratia plymuthica Strain G3: Differential Control of Motility and Biofilm Formation According to Life-Style. BMC Microbiol., 11.","DOI":"10.1186\/1471-2180-11-26"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.resmic.2006.11.008","article-title":"Characterization of a LuxI\/LuxR-Type Quorum Sensing System and N-Acyl-Homoserine Lactone-Dependent Regulation of Exo-Enzyme and Antibacterial Component Production in Serratia plymuthica RVH1","volume":"158","author":"Moons","year":"2007","journal-title":"Res. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1590\/S1517-83822011000300022","article-title":"Characterization of a Chitinase with Antifungal Activity from a Native Serratia marcescens B4A","volume":"42","author":"Zarei","year":"2011","journal-title":"Braz. J. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.micpath.2017.10.039","article-title":"Antifungal Activity and Patterns of N-Acetyl-Chitooligosaccharide Degradation via Chitinase Produced from Serratia marcescens PRNK-1","volume":"113","author":"Moon","year":"2017","journal-title":"Microb. Pathog."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1348-0421.2005.tb03734.x","article-title":"Serratia marcescens Gene Required for Surfactant Serrawettin W1 Production Encodes Putative Aminolipid Synthetase Belonging to Nonribosomal Peptide Synthetase Family","volume":"49","author":"Li","year":"2005","journal-title":"Microbiol. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Shanks, R.M.Q., Stella, N.A., Lahr, R.M., Wang, S., Veverka, T.I., Kowalski, R.P., and Liu, X. (2012). Serratamolide Is a Hemolytic Factor Produced by Serratia marcescens. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0036398"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1094\/PHYTO.2003.93.1.54","article-title":"Frequency, Diversity, and Activity of 2,4-Diacetylphloroglucinol-Producing Fluorescent Pseudomonas Spp. in Dutch Take-All Decline Soils","volume":"93","author":"Weller","year":"2003","journal-title":"Phytopathology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1055\/s-2006-924473","article-title":"Role of 2,4-Diacetylphloroglucinol-Producing Fluorescent Pseudomonas Spp. in the Defense of Plant Roots","volume":"9","author":"Weller","year":"2007","journal-title":"Plant Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1094\/MPMI.1998.11.2.144","article-title":"Natural Plant Protection by 2,4-Diacetylphloroglucinol-Producing Pseudomonas Spp. in Take-All Decline Soils","volume":"11","author":"Raaijmakers","year":"1998","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"491","DOI":"10.5423\/PPJ.FT.08.2020.0149","article-title":"Assessment of the Contribution of Antagonistic Secondary Metabolites to the Antifungal and Biocontrol Activities of Pseudomonas fluorescens NBC275","volume":"36","author":"Dutta","year":"2020","journal-title":"Plant Pathol. J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1007\/s002039900127","article-title":"Mechanism, Regulation, and Ecological Role of Bacterial Cyanide Biosynthesis","volume":"173","author":"Blumer","year":"2000","journal-title":"Arch. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1002\/j.1460-2075.1989.tb03384.x","article-title":"Cyanide Production by Pseudomonas fluorescens Helps Suppress Black Root Rot of Tobacco under Gnotobiotic Conditions","volume":"8","author":"Voisard","year":"1989","journal-title":"EMBO J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.biocontrol.2017.02.008","article-title":"Hydrogen Cyanide, Which Contributes to Pseudomonas chlororaphis Strain PA23 Biocontrol, Is Upregulated in the Presence of Glycine","volume":"108","author":"Nandi","year":"2017","journal-title":"Biological Control"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1139\/w2012-004","article-title":"Hydrogen Cyanide Synthesis and Antifungal Activity of the Biocontrol Strain Pseudomonas fluorescens In5 from Greenland Is Highly Dependent on Growth Medium","volume":"58","author":"Michelsen","year":"2012","journal-title":"Can. J. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.mib.2004.10.014","article-title":"Biofilm Formation in Plant\u2013Microbe Associations","volume":"7","author":"Ramey","year":"2004","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/1462-2920.13956","article-title":"Type VI Secretion Systems in Plant-associated Bacteria","volume":"20","author":"Bernal","year":"2017","journal-title":"Environ. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2527","DOI":"10.1038\/ismej.2016.5","article-title":"Insect Pathogenicity in Plant-Beneficial Pseudomonads: Phylogenetic Distribution and Comparative Genomics","volume":"10","author":"Flury","year":"2016","journal-title":"ISME J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e000053","DOI":"10.1099\/acmi.0.000053","article-title":"Isolation and Characterization of Novel Soil- and Plant-Associated Bacteria with Multiple Phytohormone-Degrading Activities Using a Targeted Methodology","volume":"1","author":"Nascimento","year":"2019","journal-title":"Access Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3941","DOI":"10.21105\/joss.03941","article-title":"Rasusa: Randomly Subsample Sequencing Reads to a Specified Coverage","volume":"7","author":"Hall","year":"2022","journal-title":"J. Open Source Softw."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/s41587-019-0072-8","article-title":"Assembly of Long, Error-Prone Reads Using Repeat Graphs","volume":"37","author":"Kolmogorov","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1089\/omi.2008.0017","article-title":"Toward an Online Repository of Standard Operating Procedures (SOPs) for (Meta)Genomic Annotation","volume":"12","author":"Angiuoli","year":"2008","journal-title":"OMICS A J. Integr. Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"W484","DOI":"10.1093\/nar\/gkad326","article-title":"Proksee: In-Depth Characterization and Visualization of Bacterial Genomes","volume":"51","author":"Grant","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_53","first-page":"D801","article-title":"TYGS and LPSN: A Database Tandem for Fast and Reliable Genome-Based Classification and Nomenclature of Prokaryotes","volume":"50","author":"Carbasse","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.jmb.2015.11.006","article-title":"BlastKOALA and GhostKOALA: KEGG Tools for Functional Characterization of Genome and Metagenome Sequences","volume":"428","author":"Kanehisa","year":"2016","journal-title":"J. Mol. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"W46","DOI":"10.1093\/nar\/gkad344","article-title":"AntiSMASH 7.0: New and Improved Predictions for Detection, Regulation, Chemical Structures and Visualisation","volume":"51","author":"Blin","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"D678","DOI":"10.1093\/nar\/gkae1115","article-title":"MIBiG 4.0: Advancing Biosynthetic Gene Cluster Curation through Global Collaboration","volume":"53","author":"Zdouc","year":"2025","journal-title":"Nucleic Acids Res."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/7\/1052\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T10:04:14Z","timestamp":1774865054000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/7\/1052"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,29]]},"references-count":56,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["plants15071052"],"URL":"https:\/\/doi.org\/10.3390\/plants15071052","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,29]]}}}