{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:49:18Z","timestamp":1785408558546,"version":"3.56.0"},"reference-count":76,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007045","name":"Secretar\u00eda de Ciencia Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["CBF-2025-3383"],"award-info":[{"award-number":["CBF-2025-3383"]}],"id":[{"id":"10.13039\/501100007045","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007045","name":"Secretar\u00eda de Ciencia Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["CF-2023-G-731"],"award-info":[{"award-number":["CF-2023-G-731"]}],"id":[{"id":"10.13039\/501100007045","id-type":"DOI","asserted-by":"publisher"}]},{"award":["CBF-2025-3383"],"award-info":[{"award-number":["CBF-2025-3383"]}],"id":[{"id":"https:\/\/ror.org\/02ge9fk48","id-type":"ROR","asserted-by":"publisher"}]},{"award":["CF-2023-G-731"],"award-info":[{"award-number":["CF-2023-G-731"]}],"id":[{"id":"https:\/\/ror.org\/02ge9fk48","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The mycoheterotrophic plant Monotropa uniflora relies on fungal symbionts for carbon and nutrient acquisition. However, its interactions with other microbial groups, beyond ectomycorrhizal fungi, remain unexplored. Here, we characterized bacterial and fungal communities associated with M. uniflora across two compartments: ectomycorrhizospheric soil linked to the mycorrhizal network and the surface-sterilized lower stem endosphere. Microbial community composition was assessed using high-throughput amplicon sequencing of the bacterial 16S rRNA gene and the fungal ITS region. Fungal richness was consistently higher in ectomycorrhizospheric soil than in the stem endosphere, whereas bacterial alpha diversity showed no consistent differences between compartments. Multivariate analyses suggested compartment-associated patterns in both bacterial and fungal community composition. Ectomycorrhizospheric soil was dominated by saprotrophic fungal taxa and bacterial groups with predicted metabolic potential, including taxa associated with iron, sulfur and nitrogen cycling. In contrast, the lower stem endosphere was enriched in bacterial taxa commonly associated with anaerobic and nitrogen-related metabolisms. Functional predictions further suggested an increase of carbon fixation-related pathways in rhizosphere-associated bacterial communities. Together, these results indicate that M. uniflora is associated with distinct and structured microbial assemblages across soil and internal plant compartments, highlighting the predicted functional potential of bacterial communities in nutrient- and carbon-related processes in mycoheterotrophic plant\u2013soil systems alongside fungal partners.<\/jats:p>","DOI":"10.3390\/plants15081145","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T15:26:39Z","timestamp":1775661999000},"page":"1145","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bacterial and Fungal Communities Associated with the Ectomycorrhizospheric Soil and Stem Endosphere of the Mycoheterotrophic Plant Monotropa uniflora"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5680-1945","authenticated-orcid":false,"given":"Leandro Alberto","family":"N\u00fa\u00f1ez-Mu\u00f1oz","sequence":"first","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioingenier\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados, Av. Instituto Polit\u00e9cnico Nacional 2508, Col. San Pedro Zacatenco, Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda Yazm\u00edn","family":"Vargas-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioingenier\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados, Av. Instituto Polit\u00e9cnico Nacional 2508, Col. San Pedro Zacatenco, Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa Cheryn","family":"Garc\u00eda-Sierra","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioingenier\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados, Av. Instituto Polit\u00e9cnico Nacional 2508, Col. San Pedro Zacatenco, Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6144-1411","authenticated-orcid":false,"given":"Berenice","family":"Calder\u00f3n-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioingenier\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados, Av. Instituto Polit\u00e9cnico Nacional 2508, Col. San Pedro Zacatenco, Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7366-7725","authenticated-orcid":false,"given":"Beatriz","family":"Xoconostle-C\u00e1zares","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioingenier\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados, Av. Instituto Polit\u00e9cnico Nacional 2508, Col. San Pedro Zacatenco, Mexico City 07360, Mexico"},{"name":"Programa de Doctorado Transdiciplinario en Desarrollo Cient\u00edfico y Tecnol\u00f3gico para la Sociedad, Centro de Investigaci\u00f3n y de Estudios Avanzados, Av. Instituto Polit\u00e9cnico Nacional 2508, Col. San Pedro Zacatenco, Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0223-7776","authenticated-orcid":false,"given":"Roberto","family":"Ruiz-Medrano","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioingenier\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados, Av. Instituto Polit\u00e9cnico Nacional 2508, Col. San Pedro Zacatenco, Mexico City 07360, Mexico"},{"name":"Programa de Doctorado Transdiciplinario en Desarrollo Cient\u00edfico y Tecnol\u00f3gico para la Sociedad, Centro de Investigaci\u00f3n y de Estudios Avanzados, Av. Instituto Polit\u00e9cnico Nacional 2508, Col. San Pedro Zacatenco, Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Merckx, V. (2013). Mycoheterotrophy: An Introduction. Mycoheterotrophy, Springer New York.","DOI":"10.1007\/978-1-4614-5209-6"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Varma, A., Prasad, R., and Tuteja, N. (2017). Ectomycorrhizal Mushrooms: Their Diversity, Ecology and Practical Applications. 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