{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:48:10Z","timestamp":1785408490641,"version":"3.56.0"},"reference-count":63,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:00:00Z","timestamp":1775692800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32572145"],"award-info":[{"award-number":["32572145"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32572145"],"award-info":[{"award-number":["32572145"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Provincial Undergraduate Innovation Training Program Project","award":["S202510193110"],"award-info":[{"award-number":["S202510193110"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>To improve Chrysanthemum tolerance to low temperatures and its adaptability to low autumn temperatures in Northeast China, we conducted the first genome-wide identification of the heat shock transcription factors (Hsfs) in Chrysanthemum indicum under low-temperature stress. Based on genome-wide analyses, we identified 14 CiHsf genes in Chrysanthemum indicum. Based on structural characteristics, the genes were grouped into two subfamilies, comprising 10 HsfA and four HsfB members, with no representatives of the HsfC subfamily detected. CiHsf1~CiHsf14 were located on seven chromosomes, and their promoter regions harbored numerous cis-acting elements associated with responses to low temperature, hormones, and light. Tissue-specific expression profiling revealed that seven CiHsf genes were predominantly expressed in roots, two in stems, three in leaves, and two in flowers. The analysis of low-temperature expression characteristics showed that CiHsf2, CiHsf5, CiHsf8, and CiHsf10 were significantly upregulated following cold acclimation, indicating that these genes may participate in the low-temperature response mechanism of Chrysanthemum indicum. Here, we demonstrated that transient transformation of Chrysanthemum indicum with 35S:CiHsf10 reduced reactive oxygen species (ROS) accumulation under low-temperature stress, which may contribute to enhanced cold tolerance.<\/jats:p>","DOI":"10.3390\/plants15081149","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T07:48:07Z","timestamp":1775720887000},"page":"1149","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-Wide Analysis of the Hsf Family and Functional Characterization of CiHsf10 Under Low-Temperature Stress in Chrysanthemum indicum"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2551-0793","authenticated-orcid":false,"given":"Yuzhi","family":"Song","sequence":"first","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanlu","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianru","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixi","family":"Qu","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0791-4230","authenticated-orcid":false,"given":"Yun","family":"Bai","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunwei","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1038\/s41576-021-00413-0","article-title":"Abiotic stress responses in plants","volume":"23","author":"Zhang","year":"2022","journal-title":"Nat. Rev. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s10343-025-01201-0","article-title":"Plants response to different abiotic stresses","volume":"77","author":"Jan","year":"2025","journal-title":"J. Crop Health"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.tplants.2007.07.002","article-title":"Cold stress regulation of gene expression in plants","volume":"12","author":"Chinnusamy","year":"2007","journal-title":"Trends Plant Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s12229-021-09267-x","article-title":"Overview of Cold Stress Regulation in Plants","volume":"88","author":"Manasa","year":"2022","journal-title":"Bot. Rev."},{"key":"ref_5","first-page":"100109","article-title":"The heat shock transcription factors regulate response mechanisms to abiotic stresses in plants","volume":"4","author":"Wang","year":"2025","journal-title":"Crop Des."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.bbagrm.2011.10.002","article-title":"The plant heat stress transcription factor (hsf) family: Structure, function and evolution","volume":"1819","author":"Scharf","year":"2012","journal-title":"Biochim. Biophys. Acta-Gene Regul. Mech."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Guo, M., Liu, J.-H., Ma, X., Luo, D.-X., Gong, Z.-H., and Lu, M.-H. (2016). The plant heat stress transcription factors (HSFs): Structure, regulation, and function in response to abiotic Stresses. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00114"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1093\/jxb\/eraa576","article-title":"Diversity of plant heat shock factors: Regulation, interactions, and functions","volume":"72","author":"Szabados","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1379\/1466-1268(2001)006<0177:AATHST>2.0.CO;2","article-title":"Arabidopsis and the heat stress transcription factor world: How many heat stress transcription factors do we need?","volume":"6","author":"Nover","year":"2001","journal-title":"Cell Stress Chaperones"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1111\/j.1365-313X.2004.02111.x","article-title":"Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization","volume":"39","author":"Kotak","year":"2004","journal-title":"Plant J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1379\/1466-1268(1996)001<0215:THWCAP>2.3.CO;2","article-title":"The Hsf world: Classification and properties of plant heat stress transcription factors","volume":"1","author":"Nover","year":"1996","journal-title":"Cell Stress Chaperones"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1134\/S0026893323060201","article-title":"Heat shock proteins in plant protection from oxidative stress","volume":"57","author":"Yurina","year":"2023","journal-title":"Mol. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1104\/pp.111.179036","article-title":"Arabidopsis HsfB1 and HsfB2b act as repressors of the expression of heat-inducible Hsfs but positively regulate the acquired thermotolerance","volume":"157","author":"Ikeda","year":"2011","journal-title":"Plant Physiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1104\/pp.109.142125","article-title":"BOBBER1 is a noncanonical Arabidopsis small heat shock protein required for both development and thermotolerance","volume":"151","author":"Perez","year":"2009","journal-title":"Plant Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S1673-8527(08)60016-8","article-title":"Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis","volume":"35","author":"Guo","year":"2008","journal-title":"J. Genet. Genom."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1007\/s11033-014-3826-x","article-title":"Heat shock factors in carrot: Genome-wide identification, classification, and expression profiles response to abiotic stress","volume":"42","author":"Huang","year":"2015","journal-title":"Mol. Biol. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"145348","DOI":"10.1016\/j.gene.2020.145348","article-title":"Genome-wide identification, classification and expression analysis of the Hsf and Hsp70 gene families in maize","volume":"770","author":"Jiang","year":"2021","journal-title":"Gene"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Giorno, F., Guerriero, G., Baric, S., and Mariani, C. (2012). Heat shock transcriptional factors in Malus domestica: Identification, classification and expression analysis. BMC Genom., 13.","DOI":"10.1186\/1471-2164-13-639"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Li, P.S., Yu, T.F., He, G.H., Chen, M., Zhou, Y.B., Chai, S.C., Xu, Z.S., and Ma, Y.H. (2014). Genome-wide analysis of the Hsf family in soybean and functional identification of GmHsf-34 involvement in drought and heat stresses. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-1009"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1881","DOI":"10.1111\/pce.12396","article-title":"Prospects of engineering thermotolerance in crops through modulation of heat stress transcription factor and heat shock protein networks","volume":"38","author":"Fragkostefanakis","year":"2015","journal-title":"Plant Cell Environ."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Guo, M., Lu, J.P., Zhai, Y.F., Chai, W.G., Gong, Z.H., and Lu, M.H. (2015). Genome-wide analysis, expression profile of heat shock factor gene family (CaHsfs) and characterisation of CaHsfA2 in pepper (Capsicum annuum L.). BMC Plant Biol., 15.","DOI":"10.1186\/s12870-015-0512-7"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3373","DOI":"10.1093\/jxb\/erm184","article-title":"High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt\/osmotic stress tolerance and enhanced callus growth","volume":"58","author":"Ogawa","year":"2007","journal-title":"J. Exp. Bot."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Bi, H., Zhao, Y., Li, H., and Liu, W. (2020). Wheat heat shock factor TaHsfA6f increases ABA levels and enhances tolerance to multiple abiotic stresses in transgenic plants. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21093121"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.plantsci.2015.03.006","article-title":"Populus euphratica HSF binds the promoter of WRKY1 to enhance salt tolerance","volume":"235","author":"Shen","year":"2015","journal-title":"Plant Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1007\/s40415-018-0485-5","article-title":"Two HbHsfA1 and HbHsfB1 genes from the tropical woody plant rubber tree confer cold stress tolerance in Saccharomyces cerevisiae","volume":"41","author":"Deng","year":"2018","journal-title":"Braz. J. Bot."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.molp.2024.07.015","article-title":"Genetic variation in a heat shock transcription factor modulates cold tolerance in maize","volume":"17","author":"Gao","year":"2024","journal-title":"Mol. Plant"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Gan, L., Wang, J., and Yan, X. (2025). Expanding the role of HsfA9 in cold adaptation: The bermudagrass CdHsfA9 confers cold tolerance in Arabidopsis via a novel regulatory module. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1731555"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1111\/tpj.16854","article-title":"The haplotype-resolved genome of diploid Chrysanthemum indicum unveils new acacetin synthases genes and their evolutionary history","volume":"119","author":"Hou","year":"2024","journal-title":"Plant J."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Chen, S., Yu, M., Li, H., Wang, Y., Lu, Z., Zhang, Y., Liu, M., Qiao, G., Wu, L., and Han, X. (2020). SaHsfA4c from Sedum alfredii hance enhances cadmium tolerance by regulating ROS-scavenger activities and heat shock proteins expression. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.00142"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1093\/pcp\/pcr045","article-title":"HsfA1d and HsfA1e involved in the transcriptional regulation of HsfA2 function as key regulators for the Hsf signaling network in response to environmental stress","volume":"52","author":"Nosaka","year":"2011","journal-title":"Plant Cell Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1104\/pp.114.237891","article-title":"The heat shock factor A4A confers salt tolerance and is regulated by oxidative stress and the mitogen-activated protein kinases MPK3 and MPK6","volume":"165","author":"Papdi","year":"2014","journal-title":"Plant Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1093\/pcp\/pcx160","article-title":"SUMO E3 ligase SlSIZ1 facilitates heat tolerance in tomato","volume":"59","author":"Zhang","year":"2018","journal-title":"Plant Cell Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1007\/s10811-024-03402-y","article-title":"Identification and characterization of a heat shock transcription factor in the marine red alga Pyropia yezoensis (Rhodophyta)","volume":"37","author":"Wi","year":"2025","journal-title":"J. Appl. Phycol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1093\/gbe\/evp040","article-title":"Paleopolyploidy in the brassicales: Analyses of the cleome transcriptome elucidate the history of genome duplications in Arabidopsis and other brassicales","volume":"1","author":"Barker","year":"2009","journal-title":"Genome Biol. Evol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1073\/pnas.0908007107","article-title":"Angiosperm genome comparisons reveal early polyploidy in the monocot lineage","volume":"107","author":"Tang","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.molp.2018.02.009","article-title":"Double the genome, double the fun: Genome duplications in angiosperms","volume":"11","author":"Jiao","year":"2018","journal-title":"Mol. Plant"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Feng, H., Fan, W., Liu, M., Huang, J., Li, B., Sang, Q., and Song, B. (2025). Cross-species single-nucleus analysis reveals the potential role of whole-genome duplication in the evolution of maize flower development. BMC Genom., 26.","DOI":"10.1186\/s12864-024-11186-1"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Ma, J., Zhang, G., Ye, Y., Shang, L., Hong, S., Ma, Q., Zhao, Y., and Gu, C. (2022). Genome-Wide identification and expression analysis of HSF transcription factors in alfalfa (Medicago sativa) under abiotic stress. Plants, 11.","DOI":"10.3390\/plants11202763"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Li, G., Shi, X., Lin, Q., Lv, M., Chen, J., Wen, Y., Feng, Z., Azam, S.M., Cheng, Y., and Wang, S. (2025). Genome-Wide identification and expression analysis of heat shock transcription factors in Camellia sinensis under abiotic stress. Plants, 14.","DOI":"10.3390\/plants14050697"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"107971","DOI":"10.1016\/j.plaphy.2023.107971","article-title":"An insight into Pisum sativum HSF gene family-Genome-wide identification, phylogenetic, expression, and analysis of transactivation potential of pea heat shock transcription factor","volume":"202","author":"Kanwar","year":"2023","journal-title":"Plant Physiol. Biochem. PPB"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Yang, X., Wang, S., Cai, J., Zhang, T., Yuan, D., and Li, Y. (2024). Genome-wide identification, phylogeny and expression analysis of Hsf gene family in Verbena bonariensis under low-temperature stress. BMC Genom., 25.","DOI":"10.1186\/s12864-024-10612-8"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Mu, X., Zhang, J., Wang, C., Chen, L., Zhang, J., Wang, P., Zhang, J., Zhang, B., and Du, J. (2025). Whole-genome identification of HSF family genes in Cerasus humilis and expression analysis under high-temperature stress. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1553187"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Ren, Y., Ma, R., Xie, M., Fan, Y., Feng, L., Chen, L., Yang, H., Wei, X., Wang, X., and Liu, K. (2023). Genome-wide identification, phylogenetic and expression pattern analysis of HSF family genes in the Rye (Secale cereale L.). BMC Plant Biol., 23.","DOI":"10.1186\/s12870-023-04418-1"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Shen, C., and Yuan, J. (2020). Genome-wide characterization and expression analysis of the heat shock transcription factor family in pumpkin (Cucurbita moschata). BMC Plant Biol., 20.","DOI":"10.1186\/s12870-020-02683-y"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"15682","DOI":"10.1073\/pnas.2535513100","article-title":"The early stages of duplicate gene evolution","volume":"100","author":"Moore","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1111\/nph.15011","article-title":"Constraining uncertainty in the timescale of angiosperm evolution and the veracity of a Cretaceous Terrestrial Revolution","volume":"218","author":"Schneider","year":"2018","journal-title":"New Phytol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"19363","DOI":"10.1073\/pnas.0708072104","article-title":"Using plastid genome-scale data to resolve enigmatic relationships among basal angiosperms","volume":"104","author":"Moore","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1038\/nature22380","article-title":"The sunflower genome provides insights into oil metabolism, flowering and Asterid evolution","volume":"546","author":"Badouin","year":"2017","journal-title":"Nature"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Han, Z., Zhang, C., Zhang, H., Duan, Y., Zou, Z., Zhou, L., Zhu, X., Fang, W., and Ma, Y. (2022). CsMYB transcription factors participate in jasmonic acid signal transduction in response to cold stress in tea plant (Camellia sinensis). Plants, 11.","DOI":"10.3390\/plants11212869"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3656","DOI":"10.1038\/s41467-021-24018-7","article-title":"Spatial regulation of thermomorphogenesis by HY5 and PIF4 in Arabidopsis","volume":"12","author":"Lee","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"102241","DOI":"10.1016\/j.pbi.2022.102241","article-title":"Deciphering the molecular basis of tissue-specific gene expression in plants: Can synthetic biology help?","volume":"68","author":"Yaschenko","year":"2022","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Liu, H., Li, X., Zi, Y., Zhao, G., Zhu, L., Hong, L., Li, M., Wang, S., Long, R., and Kang, J. (2023). Characterization of the heat shock transcription factor family in Medicago sativa L. and its potential roles in response to Abiotic stresses. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241612683"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1111\/j.1365-313X.2007.03334.x","article-title":"A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis","volume":"53","author":"Schramm","year":"2008","journal-title":"Plant J."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"kiaf540","DOI":"10.1093\/plphys\/kiaf540","article-title":"Chloroplast CASEIN KINASE 2 enhances maize thermotolerance via sHSP26 phosphorylation and retrograde HSFA3 regulation","volume":"199","author":"Zhao","year":"2025","journal-title":"Plant Physiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1093\/hr\/uhae238","article-title":"CGD: A multi-omics database for Chrysanthemum genomic and biological research","volume":"11","author":"Ye","year":"2024","journal-title":"Hortic. Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"D412","DOI":"10.1093\/nar\/gkaa913","article-title":"Pfam: The protein families database in 2021","volume":"49","author":"Mistry","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"W216","DOI":"10.1093\/nar\/gkab225","article-title":"Expasy, the Swiss Bioinformatics Resource Portal, as designed by its users","volume":"49","author":"Duvaud","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_58","first-page":"1090","article-title":"Cell-PLoc 2.0: An improved package of web-servers for predicting subcellular localization of proteins in various organisms","volume":"2","author":"Chou","year":"2010","journal-title":"Nat. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mo. Biol. Evol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"W39","DOI":"10.1093\/nar\/gkv416","article-title":"The MEME Suite","volume":"43","author":"Bailey","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1016\/j.molp.2023.09.010","article-title":"TBtools-II: A \u201cone for all, all for one\u201d bioinformatics platform for biological big-data mining","volume":"16","author":"Chen","year":"2023","journal-title":"Mol. Plant."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1007\/s12033-011-9394-6","article-title":"Reference gene selection for quantitative real-time PCR in Chrysanthemum subjected to biotic and abiotic stress","volume":"49","author":"Gu","year":"2011","journal-title":"Mol. Biotechnol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1104\/pp.98.1.198","article-title":"An Improved Method for Using Electrolyte Leakage to Assess Membrane Competence in Plant Tissues","volume":"98","author":"Whitlow","year":"1992","journal-title":"Plant Physiol."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/8\/1149\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T04:19:37Z","timestamp":1775881177000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/8\/1149"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,9]]},"references-count":63,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["plants15081149"],"URL":"https:\/\/doi.org\/10.3390\/plants15081149","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,9]]}}}