{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:31:56Z","timestamp":1785407516779,"version":"3.56.0"},"reference-count":43,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:00:00Z","timestamp":1775692800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"crossref","award":["22\u201374\u201310001-\u03a0"],"award-info":[{"award-number":["22\u201374\u201310001-\u03a0"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"crossref"}]},{"award":["22\u201374\u201310001-\u03a0"],"award-info":[{"award-number":["22\u201374\u201310001-\u03a0"]}],"id":[{"id":"https:\/\/ror.org\/03y2gwe85","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>This study investigates the impact of inoculating seeds with bacterial endophytes isolated from Vitis amurensis Rupr. on endophytic community composition in Arabidopsis thaliana (L.) Heynh. Ten bacterial isolates of the genera Agrobacterium, Bacillus, Curtobacterium, Erwinia, Frondihabitans, Gordonia, Pantoea, Pseudomonas, Sphingomonas, and Xanthomonas were applied to seeds and some visible phenotypic effects were observed on plant growth after two weeks. High-throughput sequencing of 16S rRNA revealed that the native endophytic microbiome of A. thaliana was dominated by Gammaproteobacteria, Actinomycetes, Bacteroidia, and Alphaproteobacteria. The key families were Microscillaceae, Chitinophagaceae, Rhizobiaceae, Rhodanobacteraceae, Nocardioi-daceae, Nocardiaceae, Xanthomonadaceae, Devosiaceae, Microbacteriaceae, Crocinitomi-caceae, Pseudomonadaceae, Solimonadaceae, Comamonadaceae, Caulobacteraceae, and Micrococcaceae. Arabidopsis seed inoculation with Agrobacterium sp. R8SCh-B12, Curtobacterium sp. P7SA-B3, and Gordonia aichiensis P6PL2 significantly reduced alpha diversity (Shannon index) and altered beta diversity relative to controls, indicating strong community restructuring. These three isolates, along with Pseudomonas sp. R8SCh-B2, Sphingomonas sp. RA62c-B5, Xanthomonas sp. R7SCh-B6, and Bacillus velezensis AMR25, successfully colonized the plant tissues, as evidenced by significant increases in genus-specific amplicon sequence variants, ASVs (up to 17,820-fold for Curtobacterium sp. ASV33). In contrast, Pantoea sp. P7SCH-B5, Erwinia sp. R8SCh-B3, and Frondihabitans sp. RA62c-B2 failed to colonize A. thaliana, despite being applied to the seeds, suggesting the existence of mechanisms restraining colonization. These findings demonstrate that only a subset of grapevine-derived endophytes can effectively colonize A. thaliana, and that successful colonization correlates with significant shifts in the native microbiome, even in the absence of overt phenotypic changes. This emphasizes the importance of strain-specific compatibility in plant\u2013endophyte interactions. Thus, we report the first descriptions of several novel endophytes that colonized Arabidopsis plants and establish a convenient model to investigate plant\u2013bacterial interactions.<\/jats:p>","DOI":"10.3390\/plants15081151","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T07:48:07Z","timestamp":1775720887000},"page":"1151","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ability of Different Bacteria from Grapevine to Colonize Arabidopsis thaliana Plants"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2549-8568","authenticated-orcid":false,"given":"Olga A.","family":"Aleynova","sequence":"first","affiliation":[{"name":"Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity of Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4671-3586","authenticated-orcid":false,"given":"Alexey A.","family":"Ananev","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity of Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolay N.","family":"Nityagovsky","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity of Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1036-680X","authenticated-orcid":false,"given":"Andrey R.","family":"Suprun","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity of Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina A.","family":"Beresh","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity of Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia"},{"name":"Advanced Engineering School, Far Eastern Federal University, 690922 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0516-6144","authenticated-orcid":false,"given":"Alexandra S.","family":"Dubrovina","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity of Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1025-6491","authenticated-orcid":false,"given":"Konstantin V.","family":"Kiselev","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity of Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia"},{"name":"Institute of the World Ocean, Far Eastern Federal University, 690922 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1007\/s10482-020-01429-y","article-title":"Endophytic microbes: Biodiversity, plant growth-promoting mechanisms and potential applications for agricultural sustainability","volume":"113","author":"Rana","year":"2020","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Kandel, S.L., Joubert, P.M., and Doty, S.L. 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