{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:15:59Z","timestamp":1785417359645,"version":"3.56.0"},"reference-count":24,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005076","name":"Agricultural Research Development Agency","doi-asserted-by":"publisher","award":["CRP6305030590"],"award-info":[{"award-number":["CRP6305030590"]}],"id":[{"id":"10.13039\/501100005076","id-type":"DOI","asserted-by":"publisher"}]},{"award":["CRP6305030590"],"award-info":[{"award-number":["CRP6305030590"]}],"id":[{"id":"https:\/\/ror.org\/01shbv660","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The Wannachawee Recipe (WCR) is a traditional Thai herbal formulation with a clinical history of use in psoriasis. An observational study conducted at Prapokklao Hospital reported that 93% of psoriasis patients showed good clinical responses. However, the absence of standardized quality control parameters remains a critical barrier to its pharmaceutical reproducibility, safety, and integration into mainstream clinical practice. This study established robust quality specifications and a phytochemical profiling for WCR, in accordance with the Thai Herbal Pharmacopoeia (THP) guidelines, to support its development from traditional use to a standardized therapeutic agent. A multimodal analytical approach was employed, integrating microscopic characterization, physicochemical evaluation, and advanced instrumental techniques. Phytochemical characterization was conducted using High-Performance Liquid Chromatography (HPLC) fingerprinting and Compact Mass Spectrometry (CMS). A validated HPLC method was developed to quantify trans-p-coumaryl alcohol, a key bioactive marker. Anti-inflammatory activity was further assessed by measuring inhibition of nitric oxide production. Physicochemical analysis established rigorous benchmarks, including ethanol-soluble extractive (8.73 \u00b1 0.15% w\/w), water-soluble extractive (18.89 \u00b1 0.09% w\/w), and loss on drying (&lt;10%), which ensure long-term stability and microbial safety. CMS analysis successfully identified key chemical constituents, including alpha-amyrin, stemone, protocatechuic acid, and trans-p-coumaryl alcohol. HPLC fingerprinting demonstrated high batch-to-batch consistency, while quantitative analysis determined a trans-p-coumaryl alcohol content of 8.77 mg\/g extract. Critically, biological evaluation showed that WCR exhibited potent anti-inflammatory activity by inhibiting nitric oxide production, with a superior inhibitory effect compared with the reference drug indomethacin. This study provides a preliminary scientific framework for the standardization of WCR. It defines precise quality specifications and a potential bioactive marker, establishing the rigor needed for regulatory certification and industrial production. This work connects traditional Thai medicine with evidence-based pharmaceutics, positioning WCR as a promising therapy for psoriasis.<\/jats:p>","DOI":"10.3390\/plants15091344","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T13:26:41Z","timestamp":1777382801000},"page":"1344","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bridging Traditional Wisdom and Evidence-Based Pharmaceutics: Comprehensive Specification and Biological Activity of the Wannachawee Recipe for Psoriasis"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5648-0238","authenticated-orcid":false,"given":"Supreeya","family":"Tantipat","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wannaree","family":"Charoensup","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kongkiat","family":"Trisuwan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand"},{"name":"Research Laboratory on Advanced Materials for Sensor and Biosensor Innovation, Materials Science Research Center, Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9499-5114","authenticated-orcid":false,"given":"Phraepakaporn","family":"Kunnaja","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9620-4590","authenticated-orcid":false,"given":"Seewaboon","family":"Sireeratawong","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand"},{"name":"Clinical Research Center for Food and Herbal Product Trials and Development (CR-FAH), Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surapol","family":"Natakankitkul","sequence":"additional","affiliation":[{"name":"Division of Herbal Innovation for Sustainable Quality of Life, Graduate School, Payap University, Chiang Mai 50000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surasak","family":"Imiam","sequence":"additional","affiliation":[{"name":"Department of Thai Traditional Medicine, Phrapokklao Hospital, Chanthaburi 22000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apinya","family":"Rachkeeree","sequence":"additional","affiliation":[{"name":"Queen Sirikit Botanic Garden, The Botanical Garden Organization, Chiang Mai 50180, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1884-727X","authenticated-orcid":false,"given":"Ratchuporn","family":"Suksathan","sequence":"additional","affiliation":[{"name":"Queen Sirikit Botanic Garden, The Botanical Garden Organization, Chiang Mai 50180, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunee","family":"Chansakaow","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5906539","DOI":"10.1155\/2017\/5906539","article-title":"Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes","volume":"2017","author":"Wongnoppavich","year":"2017","journal-title":"Evid. 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