{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T05:05:33Z","timestamp":1779512733925,"version":"3.53.1"},"reference-count":20,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Lumnitzera littorea is a rare, conservation-relevant mangrove tree with discontinuous records in southern Vietnam, but population-level genetic evidence remains limited. This study combined nuclear rDNA ITS and ISSR markers to distinguish species-level support from population-level comparisons. Can Gio was treated as the focal population, while Dong Nai, Phu Quoc, and Con Dao were used as comparison populations. The 16 study-generated ITS sequences, deposited as PZ348213\u2013PZ348228, supported species-level separation between L. littorea and L. racemosa, with a between-species p-distance of 0.0459 and 32 fixed diagnostic sites across the 697 bp core. The ISSR matrix comprised 115 individuals and 81 loci, and population analyses were restricted to 110 L. littorea individuals. Within this dataset, Dong Nai and Can Gio showed higher ISSR diversity than Con Dao and Phu Quoc. AMOVA indicated significant differentiation among populations (Phi_PT = 0.255, p = 0.001), with 74.5% of variation retained within populations; a mainland\u2013island grouping was also significant (Phi_PT = 0.242, p = 0.001). Repeated n = 20 subsampling retained the relative diversity pattern. The results provide a regional ISSR baseline for conservation-genetics interpretation and support broad representation of local source trees when collecting conservation material; however, they do not define formal management units, official seed-transfer zones, or deep phylogeographic history.<\/jats:p>","DOI":"10.3390\/plants15101569","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T09:29:58Z","timestamp":1779355798000},"page":"1569","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["ITS-Supported Species Discrimination and ISSR-Based Genetic Diversity and Population Differentiation of Lumnitzera littorea in Southern Vietnam"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4470-3006","authenticated-orcid":false,"given":"Duc-Hoan","family":"Huynh","sequence":"first","affiliation":[{"name":"Management Board of Protection and Special-Use Forests of Ho Chi Minh City, 176 Hai Ba Trung Street, Tan Dinh Ward, Ho Chi Minh City 71008, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"The-Kiet","family":"Bui-Nguyen","sequence":"additional","affiliation":[{"name":"Management Board of Protection and Special-Use Forests of Ho Chi Minh City, 176 Hai Ba Trung Street, Tan Dinh Ward, Ho Chi Minh City 71008, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4533-2347","authenticated-orcid":false,"given":"Huu-Nghia","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City, 227 Nguyen Van Cu Street, Cho Quan Ward, Ho Chi Minh City 72722, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanh-Cong","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Institute of Applied Research and Technology Transfer, Ho Chi Minh City University of Industry and Trade (HUIT), 93 Tan Ky Tan Quy Street, Tan Son Nhi Ward, Ho Chi Minh City 72008, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3563-2887","authenticated-orcid":false,"given":"Hoang-Dung","family":"Tran","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Environment, Ho Chi Minh City University of Industry and Trade (HUIT), 140 Le Trong Tan Street, Tay Thanh Ward, Ho Chi Minh City 72009, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Tomlinson, P.B. (2016). The Botany of Mangroves, Cambridge University Press. [2nd ed.].","DOI":"10.1017\/CBO9781139946575"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.15625\/2615-9023\/20802","article-title":"Species composition and diversity of mangrove communities associated with Lumnitzera littorea in southern Vietnam","volume":"47","author":"Quach","year":"2025","journal-title":"Acad. J. Biol."},{"key":"ref_3","first-page":"81","article-title":"Species composition and newly recorded distribution of Lumnitzera littorea (Jack) Voigt in Long Thanh protection forest, Dong Nai province","volume":"5","author":"Nguyen","year":"2020","journal-title":"Vietnam J. For. Sci."},{"key":"ref_4","first-page":"453","article-title":"Study on morphological and anatomical characteristics of Lumnitzera littorea distributed in the South of Vietnam","volume":"18","author":"Quach","year":"2021","journal-title":"Ho Chi Minh City Univ. Educ. J. Sci."},{"key":"ref_5","first-page":"1842","article-title":"Physicochemical properties of the soil in Lumnitzera littorea communities distributed in the South of Vietnam","volume":"19","author":"Quach","year":"2022","journal-title":"Ho Chi Minh City Univ. Educ. J. Sci."},{"key":"ref_6","first-page":"299","article-title":"Culture of Lumnitzera littorea (Jack) Voigt embryos","volume":"17","author":"Nguyen","year":"2017","journal-title":"Vietnam J. Mar. Sci. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s00227-006-0357-6","article-title":"Conservation genetics of Lumnitzera littorea (Combretaceae), an endangered mangrove, from the Indo-West Pacific","volume":"150","author":"Su","year":"2007","journal-title":"Mar. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Guo, W., Banerjee, A.K., Wu, H., Ng, W.L., Feng, H., Qiao, S., Liu, Y., and Huang, Y. (2021). Contrasting phylogeographic patterns in Lumnitzera mangroves across the Indo-West Pacific. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.637009"},{"key":"ref_9","first-page":"311","article-title":"DNA barcoding of the true mangrove plants, a selection of genetic markers","volume":"17","author":"Nguyen","year":"2017","journal-title":"J. Mar. Sci. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"19641","DOI":"10.1073\/pnas.1104551108","article-title":"Comparative analysis of a large dataset indicates that ITS should be incorporated into the core barcode for seed plants","volume":"108","author":"Li","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Mao, X., Xie, W., Li, X., Shi, S., and Guo, Z. (2021). Establishing community-wide DNA barcode references for conserving mangrove forests in China. BMC Plant Biol., 21.","DOI":"10.1186\/s12870-021-03349-z"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1006\/geno.1994.1151","article-title":"Genome fingerprinting by simple sequence repeat-anchored PCR amplification","volume":"20","author":"Zietkiewicz","year":"1994","journal-title":"Genomics"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1023\/A:1020691618797","article-title":"Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding","volume":"128","author":"Reddy","year":"2002","journal-title":"Euphytica"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/S0076-6879(05)95009-X","article-title":"Amplified fragment length polymorphism and inter-simple sequence repeat markers in plant biology","volume":"395","author":"Wolfe","year":"2005","journal-title":"Methods Enzymol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.aquabot.2011.03.001","article-title":"Molecular confirmation of natural hybridization between Lumnitzera racemosa and Lumnitzera littorea","volume":"95","author":"Guo","year":"2011","journal-title":"Aquat. Bot."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.aquabot.2007.12.013","article-title":"Molecular ecology and biogeography of mangrove trees towards conceptual insights on gene flow and barriers: A review","volume":"89","author":"Triest","year":"2008","journal-title":"Aquat. Bot."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Yan, Y.-B., Duke, N.C., and Sun, M. (2016). Comparative analysis of the pattern of population genetic diversity in three Indo-West Pacific Rhizophora mangrove species. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.01434"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s10592-006-9151-8","article-title":"Phylogeography of Ceriops tagal (Rhizophoraceae) in Southeast Asia: The land barrier of the Malay Peninsula has caused population differentiation between the Indian Ocean and South China Sea","volume":"8","author":"Liao","year":"2007","journal-title":"Conserv. Genet."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Triest, L., Satyanarayana, B., Delange, O., Sarker, K.K., Sierens, T., and Dahdouh-Guebas, F. (2021). Barrier to gene flow of grey mangrove Avicennia marina populations in the Malay Peninsula as revealed from nuclear microsatellites and chloroplast haplotypes. Front. Conserv. Sci., 2.","DOI":"10.3389\/fcosc.2021.727819"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.1093\/bioinformatics\/bts460","article-title":"GenAlEx 6.5: Genetic analysis in Excel-population genetic software for teaching and research-an update","volume":"28","author":"Peakall","year":"2012","journal-title":"Bioinformatics"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/10\/1569\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T04:17:34Z","timestamp":1779509854000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/10\/1569"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,21]]},"references-count":20,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["plants15101569"],"URL":"https:\/\/doi.org\/10.3390\/plants15101569","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,21]]}}}