{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T19:52:09Z","timestamp":1789069929399,"version":"build-2803163510"},"reference-count":32,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T00:00:00Z","timestamp":1780185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of National Key Laboratory of Tropical Crop Breeding","award":["NKLTCBZRJJ3"],"award-info":[{"award-number":["NKLTCBZRJJ3"]}]},{"name":"Hainan Provincial Natural Science Foundation of China","award":["324QN328"],"award-info":[{"award-number":["324QN328"]}]},{"name":"Hainan Provincial Natural Science Foundation of China","award":["323QN296"],"award-info":[{"award-number":["323QN296"]}]},{"name":"Science and Technology Talent Innovation Project of Hainan Province, Comprehensive Genome Design Breeding for Key Tropical Crops and Creation of New Varieties","award":["KJRC2023A04"],"award-info":[{"award-number":["KJRC2023A04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Potato (Solanum tuberosum) is a vital global non-cereal food crop severely threatened by bacterial wilt, caused by Ralstonia solanacearum(R. solanacearum). Conventional diagnostics like PCR and ELISA, though effective, are destructive and time-consuming, limiting large-scale field applications. This study investigates hyperspectral imaging (HSI) as a non-invasive, rapid, and accurate alternative for early detection and severity grading of potato bacterial wilt. Using a portable HSI system (400\u20131000 nm), spectral data were collected from inoculated potato plants (\u2018Longshu No. 7\u2019) at 0, 24, 48, and 72 h post-inoculation, alongside disease severity assessment (grades 0\u20134). After comprehensive spectral preprocessing and feature band extraction via Competitivse Adaptive Reweighted Sampling (CARS), we developed two distinct sets of models: one for early detection (temporal classification) using Partial Least Squares-Discriminant Analysis (PLS-DA) and Principal Component Analysis-Linear Discriminant Analysis (PCA-LDA), and another for severity grading. The SNV + SG + MC + PLS-DA model achieved exceptional accuracy, exceeding 97% for early detection, while the MSC + SG + MC + CARS + PLS-DA model yielded &gt;97% accuracy for severity grading. These results were supported by low misclassification rates in confusion matrices. This work establishes a robust HSI-based framework for high-throughput screening of resistant potato germplasm and advances precision agriculture strategies for bacterial wilt management.<\/jats:p>","DOI":"10.3390\/plants15111706","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T08:09:16Z","timestamp":1780301356000},"page":"1706","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Hyperspectral Imaging for Early Detection and Severity Grading of Potato Bacterial Wilt"],"prefix":"10.3390","volume":"15","author":[{"given":"Zhuo","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tropical Crop Breeding, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China"},{"name":"Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs\/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhendong","family":"Lan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crop Breeding, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China"},{"name":"College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi-Ou","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crop Breeding, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China"},{"name":"Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs\/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6563-6582","authenticated-orcid":false,"given":"Xi","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crop Breeding, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China"},{"name":"Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs\/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6083-394X","authenticated-orcid":false,"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crop Breeding, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China"},{"name":"Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs\/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xidan","family":"Pang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crop Breeding, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China"},{"name":"Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs\/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crop Breeding, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China"},{"name":"Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs\/Key Laboratory of Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1016\/j.molp.2022.06.009","article-title":"Genome Architecture and Tetrasomic Inheritance of Autotetraploid Potato","volume":"15","author":"Bao","year":"2022","journal-title":"Mol. 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