{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:03:54Z","timestamp":1785402234181,"version":"3.56.0"},"reference-count":52,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Talent Project of Gansu Province","award":["2025RCXM104"],"award-info":[{"award-number":["2025RCXM104"]}]},{"name":"Gansu Academy of Agricultural Sciences","award":["2026GAAS08"],"award-info":[{"award-number":["2026GAAS08"]}]},{"award":["2026GAAS08"],"award-info":[{"award-number":["2026GAAS08"]}],"id":[{"id":"https:\/\/ror.org\/001tdwk28","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Brassica napus reproductive development and abiotic stress tolerance are critical for yield and quality, and characterizing key transcription factor families is vital for molecular breeding. Here, based on the B. napus cv. Darmor-bzh V5 reference genome, we systematically identified and analyzed the BnaS1fa gene family, uncovering 12 members. Their encoded proteins are mostly small, alkaline, stable, and hydrophilic, with a few having ultra-long structures. Phylogenetic analysis clustered them into three subfamilies; conserved motif and gene structure analyses revealed high overall family conservation with partial member differentiation. Promoter cis-acting element analysis showed enrichment in light, hormone, and stress-responsive elements. Chromosomal localization and intraspecific collinearity analyses indicated the family mainly derived from homologous fragment retention in A and C subgenomes. Transcriptome data demonstrated high BnaS1fa expression in late seed and silique development, with prominent heat stress responses. RT-qPCR, subcellular localization and transcriptional activity assays confirmed BnaS1fa9 and BnaS1fa10 as nuclear-localized transcription factors with heat stress-induced expression. This study elucidates BnaS1fa molecular characteristics and its potential roles in reproductive development and heat stress response, providing candidate genes for B. napus stress-resistant molecular breeding. Further functional validation of these key genes will facilitate the dissection of their precise regulatory mechanisms governing heat stress tolerance and reproductive growth, which can be ultimately applied to advance the genetic improvement of rapeseed stress resistance and yield performance.<\/jats:p>","DOI":"10.3390\/plants15121808","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T06:53:10Z","timestamp":1781247190000},"page":"1808","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-Wide Identification of S1fa Transcription Factors in Brassica napus and Screening of Key Genes BnaS1fa9 and BnaS1fa10 Responsive to Salt, Heat and Cold Stresses"],"prefix":"10.3390","volume":"15","author":[{"given":"Ruisheng","family":"Qi","sequence":"first","affiliation":[{"name":"Agricultural Technology Extension Service Center of Linxia Hui Autonomous Prefecture, Linxia 731100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Mi","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics & Germplasm Innovation and Utilization, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of China, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunmiao","family":"Xu","sequence":"additional","affiliation":[{"name":"Economic Crops Technology Extension Station of Anding District, Dingxi 743000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingfan","family":"Guo","sequence":"additional","affiliation":[{"name":"Agricultural Technology Extension Service Center of Linxia Hui Autonomous Prefecture, Linxia 731100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Dong","sequence":"additional","affiliation":[{"name":"Gansu Academy of Agricultural Sciences, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Chen","sequence":"additional","affiliation":[{"name":"Agricultural Technology Extension Service Center of Linxia Hui Autonomous Prefecture, Linxia 731100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianye","family":"Wei","sequence":"additional","affiliation":[{"name":"Linxia Hui Autonomous Prefecture Academy of Agricultural Sciences, Linxia 731100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renmei","family":"Dang","sequence":"additional","affiliation":[{"name":"Linxia Hui Autonomous Prefecture Academy of Agricultural Sciences, Linxia 731100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaonan","family":"Wu","sequence":"additional","affiliation":[{"name":"Linxia Hui Autonomous Prefecture Academy of Agricultural Sciences, Linxia 731100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Dong","sequence":"additional","affiliation":[{"name":"Gansu Academy of Agricultural Sciences, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huizhen","family":"Ma","sequence":"additional","affiliation":[{"name":"Agricultural Technology Extension Service Center of Linxia Hui Autonomous Prefecture, Linxia 731100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyang","family":"Ma","sequence":"additional","affiliation":[{"name":"Agricultural Technology Extension Service Center of Linxia Hui Autonomous Prefecture, Linxia 731100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhao, H., Niu, Y., Dong, H., Jia, Y., and Wang, Y. (2021). Characterization of the Function of Two S1Fa-Like Family Genes From Populus trichocarpa. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.753099"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s40659-018-0195-2","article-title":"The Physiological and Molecular Mechanism of Brassinosteroid in Response to Stress: A Review","volume":"51","author":"Anwar","year":"2018","journal-title":"Biol. Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Nuruzzaman, M., Sharoni, A.M., and Kikuchi, S. (2013). Roles of NAC Transcription Factors in the Regulation of Biotic and Abiotic Stress Responses in Plants. Front. Microbiol., 4.","DOI":"10.3389\/fmicb.2013.00248"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Anwar, A., and Kim, J.-K. (2020). Transgenic Breeding Approaches for Improving Abiotic Stress Tolerance: Recent Progress and Future Perspectives. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21082695"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Cheng, Y., Liang, C., Qiu, Z., Zhou, S., Liu, J., Yang, Y., Wang, R., Yin, J., Ma, C., and Cui, Z. (2023). Jasmonic Acid Negatively Regulates Branch Growth in Pear. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1105521"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Liu, C., Jiang, X., Liu, S., Liu, Y., Li, H., Wang, Z., Kan, J., Yang, Q., and Li, X. (2024). Comprehensive Evolutionary Analysis of the SMXL Gene Family in Rosaceae: Further Insights into Its Origin, Expansion, Diversification, and Role in Regulating Pear Branching. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25052971"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1613131","DOI":"10.1080\/15592324.2019.1613131","article-title":"Research Advances of MYB Transcription Factors in Plant Stress Resistance and Breeding","volume":"14","author":"Li","year":"2019","journal-title":"Plant Signal. Behav."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Sun, S., Chen, H., Yang, Z., Lu, J., Wu, D., Luo, Q., Jia, J., and Tan, J. (2022). Identification of WRKY Transcription Factor Family Genes in Pinus massoniana lamb. and Their Expression Patterns and Functions in Response to Drought Stress. BMC Plant Biol., 22.","DOI":"10.1186\/s12870-022-03802-7"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Gr\u0105dzka, K., Biega\u0144ska, M., Koczyk, G., M\u0142odzi\u0144ska, A., Paw\u0142owicz, I., and Kie\u0142bowicz-Matuk, A. (2025). CHIP-Seq and Transcriptomics Reveal a New Role of Circadian-Regulated StBBX24 Protein in Potato Reproduction. BMC Plant Biol., 25.","DOI":"10.1186\/s12870-025-07811-0"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Wang, C., Lan, M., Xiao, M., Peng, Y., Pan, H., Deng, J., and Wu, W. (2025). Genome-Wide Identification of GATA Family Genes in Sweet Potato (Ipomoea batatas L.) and Their Expression Patterns under Abiotic Stress. Front. Genet., 16.","DOI":"10.3389\/fgene.2025.1635749"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"100084","DOI":"10.1016\/j.ncrops.2025.100084","article-title":"Genome-Wide Analysis of the WRKY Gene Family in Lily and Functional Characterization of LdWRKY87 in Response to Heat Stress","volume":"3","author":"Xu","year":"2026","journal-title":"New Crops"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1111\/pbi.14056","article-title":"The miR156b\u2013GmSPL2b Module Mediates Male Fertility Regulation of Cytoplasmic Male Sterility-based Restorer Line under High-temperature Stress in Soybean","volume":"21","author":"Ding","year":"2023","journal-title":"Plant Biotechnol. J."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Liu, C., Fan, E., Liu, Y., Wang, M., Wang, Q., Wang, S., Chen, S., Yang, C., You, X., and Qu, G. (2024). Genome-Wide Identification and Analysis of the EIN3\/EIL Transcription Factor Gene Family in Doubled Haploid (DH) Poplar. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25074116"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Sun, S., Liu, X., Zhang, T., Yang, H., and Yu, B. (2023). Functional Characterisation of the Transcription Factor GsWRKY23 Gene from Glycine Soja in Overexpressed Soybean Composite Plants and Arabidopsis under Salt Stress. Plants, 12.","DOI":"10.3390\/plants12173030"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"16955","DOI":"10.1038\/s41598-023-44340-y","article-title":"Genome-Wide Identification and Comprehensive Analysis of WRKY Transcription Factor Family in Safflower during Drought Stress","volume":"13","author":"Song","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"108664","DOI":"10.1016\/j.isci.2023.108664","article-title":"Overexpression and Knockdown of Cotton GhdadD Gene Reveals Its Drought and Salt Stress Tolerance Role","volume":"27","author":"Zhang","year":"2024","journal-title":"iScience"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6785","DOI":"10.1073\/pnas.1600899113","article-title":"Draft Genome of the Peanut A-Genome Progenitor (Arachis duranensis) Provides Insights into Geocarpy, Oil Biosynthesis, and Allergens","volume":"113","author":"Chen","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1093\/nar\/23.7.1165","article-title":"Molecular Cloning of a Small DNA Binding Protein with Specificity for a Tissue-Specific Negative Element within the Rps 1 Promoter","volume":"23","author":"Zhou","year":"1995","journal-title":"Nucleic Acids Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wang, T., Zou, H., Ren, S., Jin, B., and Lu, Z. (2023). Genome-Wide Identification, Characterization, and Expression Analysis of NF-Y Gene Family in Ginkgo Biloba Seedlings and GbNF-YA6 Involved in Heat-Stress Response and Tolerance. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241512284"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"23515","DOI":"10.1016\/S0021-9258(18)35869-1","article-title":"Sequence-specificInteraction between S l F, a Spinach Nuclear Factor, and a Negative &-Element Conserved in Plastid-relatedGenes","volume":"267","author":"Zhou","year":"1992","journal-title":"J. Biol. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Kim, S.-I., Lee, K.H., Kwak, J.S., Kwon, D.H., Song, J.T., and Seo, H.S. (2021). Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis. Plants, 10.","DOI":"10.3390\/plants10102181"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Anwar, A., Zhang, S., Wang, L.-X., Wang, F., He, L., and Gao, J. (2022). Genome-Wide Identification and Characterization of Chinese Cabbage S1fa Transcription Factors and Their Roles in Response to Salt Stress. Antioxidants, 11.","DOI":"10.3390\/antiox11091782"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/j.plaphy.2010.08.016","article-title":"Reactive Oxygen Species and Antioxidant Machinery in Abiotic Stress Tolerance in Crop Plants","volume":"48","author":"Gill","year":"2010","journal-title":"Plant Physiol. Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Li, P., Du, R., Li, Z., Chen, Z., Li, J., and Du, H. (2023). An Integrated Nitrogen Utilization Gene Network and Transcriptome Analysis Reveal Candidate Genes in Response to Nitrogen Deficiency in Brassica napus. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1187552"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Liang, Y., Wan, N., Cheng, Z., Mo, Y., Liu, B., Liu, H., Raboanatahiry, N., Yin, Y., and Li, M. (2017). Whole-Genome Identification and Expression Pattern of the Vicinal Oxygen Chelate Family in Rapeseed (Brassica napus L.). Front. Plant Sci., 8.","DOI":"10.3389\/fpls.2017.00745"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1126\/science.1253435","article-title":"Early Allopolyploid Evolution in the Post-Neolithic Brassica napus Oilseed Genome","volume":"345","author":"Chalhoub","year":"2014","journal-title":"Science"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Ali, E., Hussain, S., Jalal, F., Khan, M.A., Imtiaz, M., Said, F., Ismail, M., Khan, S., Ali, H.M., and Hatamleh, A.A. (2023). Salicylic Acid-Mitigates Abiotic Stress Tolerance via Altering Defense Mechanisms in Brassica napus (L.). Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1187260"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Dong, Q., Magwanga, R.O., Cai, X., Lu, P., Nyangasi Kirungu, J., Zhou, Z., Wang, X., Wang, X., Xu, Y., and Hou, Y. (2019). RNA-Sequencing, Physiological and RNAi Analyses Provide Insights into the Response Mechanism of the ABC-Mediated Resistance to Verticillium Dahliae Infection in Cotton. Genes, 10.","DOI":"10.3390\/genes10020110"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Chosyang, S., Arya, P., Kaur, M., and Singh, B. (2026). Comparative Analysis of the Aquaporin Gene Family in Cucurbitaceae: Insights into Evolutionary Patterns and Functional Diversity. BMC Plant Biol., 26.","DOI":"10.1186\/s12870-025-07818-7"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"39968","DOI":"10.1038\/s41598-025-23630-7","article-title":"Genome-Wide Analysis of NAC Transcription Factors in Grain Amaranth Reveals Structural Diversity and Regulatory Features","volume":"15","author":"Eerapagula","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Yu, W., Kong, G., Ya, H., He, L., Wu, Y., and Zhang, H. (2023). Comprehensive Analysis of the Catalase (CAT) Gene Family and Expression Patterns in Rubber Tree (Hevea brasiliensis) under Various Abiotic Stresses and Multiple Hormone Treatments. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25010070"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"36163","DOI":"10.1038\/s41598-025-18901-2","article-title":"Analysis of Expansin Genes in Cucurbitaceae and Identification of Candidate Genes for Stress Resistance","volume":"15","author":"Qi","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2224","DOI":"10.1111\/pbi.70048","article-title":"Ecotype-specific Phenolic Acid Accumulation and Root Softness in Salvia miltiorrhiza Are Driven by Environmental and Genetic Factors","volume":"23","author":"Yu","year":"2025","journal-title":"Plant Biotechnol. J."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Fern\u00e1ndez-Rold\u00e1n, P., Moreno-Recio, M.D., Spadoni-Revol, F., Molina-Hidalgo, F.J., Caballero, J.L., Mu\u00f1oz-Blanco, J., Blanco-Portales, R., and Moyano, E. (2026). Genome-Wide Identification and Expression Analysis of Tubby-like Proteins (TLPs) in Fragaria \u00d7 Ananassa Reveals Their Role in Abiotic Stress Responses. Genes, 17.","DOI":"10.3390\/genes17020158"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1093\/molbev\/msaa302","article-title":"Degree of Functional Divergence in Duplicates Is Associated with Distinct Roles in Plant Evolution","volume":"38","author":"Ezoe","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Chen, E.C., Buen Abad Najar, C.F., Zheng, C., Brandts, A., Lyons, E., Tang, H., Carretero-Paulet, L., Albert, V.A., and Sankoff, D. (2013). The Dynamics of Functional Classes of Plant Genes in Rediploidized Ancient Polyploids. BMC Bioinform., 14.","DOI":"10.1186\/1471-2105-14-S15-S19"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Hajheidari, M., Koncz, C., and Bucher, M. (2019). Chromatin Evolution-Key Innovations Underpinning Morphological Complexity. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.00454"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"uhac058","DOI":"10.1093\/hr\/uhac058","article-title":"Evolution and Functional Diversification of R2R3-MYB Transcription Factors in Plants","volume":"9","author":"Wu","year":"2022","journal-title":"Hortic. Res."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Duran, B.O.S., Garcia De La Serrana, D., Zanella, B.T.T., Perez, E.S., Mareco, E.A., Santos, V.B., Carvalho, R.F., and Dal-Pai-Silva, M. (2021). An Insight on the Impact of Teleost Whole Genome Duplication on the Regulation of the Molecular Networks Controlling Skeletal Muscle Growth. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0255006"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s13007-017-0192-4","article-title":"A Fast, Efficient Chromatin Immunoprecipitation Method for Studying Protein-DNA Binding in Arabidopsis Mesophyll Protoplasts","volume":"13","author":"Lee","year":"2017","journal-title":"Plant Methods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"D1432","DOI":"10.1093\/nar\/gkab1057","article-title":"BRAD V3.0: An Upgraded Brassicaceae Database","volume":"50","author":"Chen","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"iyaf248","DOI":"10.1093\/genetics\/iyaf248","article-title":"Recent Major Changes to TAIR: Updates to the Database, Website, and Arabidopsis Genome","volume":"232","author":"Reiser","year":"2026","journal-title":"Genetics"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"D412","DOI":"10.1093\/nar\/gkaa913","article-title":"Pfam: The Protein Families Database in 2021","volume":"49","author":"Mistry","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"W6","DOI":"10.1093\/nar\/gkl164","article-title":"BLAST: Improvements for Better Sequence Analysis","volume":"34","author":"Ye","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"W29","DOI":"10.1093\/nar\/gkr367","article-title":"HMMER Web Server: Interactive Sequence Similarity Searching","volume":"39","author":"Finn","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"W216","DOI":"10.1093\/nar\/gkab225","article-title":"Expasy, the Swiss Bioinformatics Resource Portal, as Designed by Its Users","volume":"49","author":"Duvaud","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Price, M.N., Dehal, P.S., and Arkin, A.P. (2010). FastTree 2\u2014Approximately Maximum-Likelihood Trees for Large Alignments. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0009490"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"W78","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive Tree of Life (iTOL) v6: Recent Updates to the Phylogenetic Tree Display and Annotation Tool","volume":"52","author":"Letunic","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1093\/bioinformatics\/btu817","article-title":"GSDS 2.0: An Upgraded Gene Feature Visualization Server","volume":"31","author":"Hu","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/nar\/30.1.325","article-title":"PlantCARE, a Database of Plant Cis-Acting Regulatory Elements and a Portal to Tools for in Silico Analysis of Promoter Sequences","volume":"30","author":"Lescot","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1016\/j.molp.2023.09.010","article-title":"TBtools-II: A \u201cOne for All, All for One\u201d Bioinformatics Platform for Biological Big-Data Mining","volume":"16","author":"Chen","year":"2023","journal-title":"Mol. Plant"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.molp.2023.03.007","article-title":"BnIR: A Multi-Omics Database with Various Tools for Brassica Napus Research and Breeding","volume":"16","author":"Yang","year":"2023","journal-title":"Mol. Plant"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/12\/1808\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T08:06:01Z","timestamp":1781251561000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/12\/1808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,12]]},"references-count":52,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["plants15121808"],"URL":"https:\/\/doi.org\/10.3390\/plants15121808","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,12]]}}}