{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T05:15:28Z","timestamp":1783574128358,"version":"3.55.0"},"reference-count":101,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Improved varieties strengthen cassava\u2019s roles as both a food security staple and a versatile industrial raw material across the tropical regions where it is produced. In support of this effort the International Center for Tropical Agriculture (CIAT) curates the world\u2019s largest cassava germplasm collection at its Cali, Colombia, research center. Since the first collection expeditions in 1969, a primary focus was to assemble and conserve the diversity from the crop\u2019s center of origin in the American tropics. Later additions expanded representation from Asia and Africa as secondary centers of diversity. The collection now consists mainly of landraces (about 5000 accessions), bred lines from CIAT (375) and from partner institutions (253), and related wild Manihot (377 accessions from 23 species or subspecies). Landrace diversity originated and evolved almost entirely through occasional farmer selections from seed-derived plants, which were subsequently conserved clonally over many generations. Secure ex situ conservation, first as a field collection and then in a slow-growth in vitro system, gave priority to pathogen testing and reliable culture and exchange methods. Cryopreservation research is ongoing to achieve added security and efficiency. CIAT extensively characterizes accessions through morphological, biochemical and molecular criteria. As a core goal, the collection has been a foundation for genetic improvement of the crop globally. This paper provides perspectives on the future management and use of the collection in the context of the recently established Future Seeds genebank facilities in Colombia, and new tools and technologies that support more effective conservation, evaluation and use.<\/jats:p>","DOI":"10.3390\/plants15131981","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T02:16:44Z","timestamp":1782699404000},"page":"1981","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adding Value to Cassava Genetic Resources Conserved at CIAT\u2014Part I: A Review of Fifty Years of Collection, Conservation, Characterization and Distribution"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2412-6228","authenticated-orcid":false,"given":"Clair H.","family":"Hershey","sequence":"first","affiliation":[{"name":"Independent Researcher, Formerly, Cassava Program, Centro Internacional de Agricultura Tropical (CIAT), Recta Cali-Palmira Apartado A\u00e9reo 6713, Cali 763537, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7487-9909","authenticated-orcid":false,"given":"Ericson","family":"Aranzales R.","sequence":"additional","affiliation":[{"name":"Genetic Resources Program, Centro Internacional de Agricultura Tropical (CIAT), Recta Cali-Palmira Apartado A\u00e9reo 6713, Cali 763537, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Jaramillo O.","sequence":"additional","affiliation":[{"name":"Independent Researcher, Formerly, Cassava Program, Centro Internacional de Agricultura Tropical (CIAT), Recta Cali-Palmira Apartado A\u00e9reo 6713, Cali 763537, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8235-3181","authenticated-orcid":false,"given":"Norma C.","family":"Manrique-Carpintero","sequence":"additional","affiliation":[{"name":"Genetic Resources Program, Centro Internacional de Agricultura Tropical (CIAT), Recta Cali-Palmira Apartado A\u00e9reo 6713, Cali 763537, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7065-2683","authenticated-orcid":false,"given":"Monica L.","family":"Velez-Tobon","sequence":"additional","affiliation":[{"name":"Genetic Resources Program, Centro Internacional de Agricultura Tropical (CIAT), Recta Cali-Palmira Apartado A\u00e9reo 6713, Cali 763537, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Wenzl","sequence":"additional","affiliation":[{"name":"Genetic Resources Program, Centro Internacional de Agricultura Tropical (CIAT), Recta Cali-Palmira Apartado A\u00e9reo 6713, Cali 763537, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"ref_1","unstructured":"Lynam, J., and Byerlee, D. (2017). Forever Pioneers\u2014CIAT: 50 Years Contributing to a Sustainable Food Future\u2026 and Counting, International Center for Tropical Agriculture (CIAT). CIAT Publication No. 444."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"51","DOI":"10.46265\/genresj.KZEW5023","article-title":"A historical appraisal of the tropical forages collection conserved at CIAT","volume":"1","author":"Peters","year":"2020","journal-title":"Genet. Resour."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"21","DOI":"10.46265\/genresj.WJEU8358","article-title":"History and impact of a bean (Phaseolus spp., Leguminosae, Phaseoleae) collection","volume":"2","author":"Debouck","year":"2021","journal-title":"Genet. Resour."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s001220050620","article-title":"AFLP analysis of relationships among cassava and other Manihot species","volume":"95","author":"Roa","year":"1997","journal-title":"Theor. Appl. Genet."},{"key":"ref_5","unstructured":"Fukuda, W.M.G., and Guevara, C.L. (1998). Descritores Morfol\u00f3gicos e Agron\u00f4micos para a Caracteriza\u00e7\u00e3o de Mandioca (Manihot esculenta Crantz), Embrapa."},{"key":"ref_6","unstructured":"Fukuda, W.M.G., Guevara, C., Kawuki, R., and Ferguson, M.E. (2010). Selected Morphological and Agronomic Descriptors for the Characterization of Cassava, IITA."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7469","DOI":"10.1021\/jf070633y","article-title":"Discovery of an amylose-free starch mutant in cassava (Manihot esculenta Crantz)","volume":"55","author":"Ceballos","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_8","unstructured":"Hershey, C.H. (2010). A Global Conservation Strategy for Cassava (Manihot esculenta) and Wild Manihot Species, The Crop Trust. Crop Strategy, Cassava."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Sheat, S., Zhang, X., and Winter, S. (2022). High-throughput virus screening in crosses of South American and Afri-can cassava germplasm reveals broad-spectrum resistance against viruses causing cassava brown streak disease and cassava mosaic disease. Agronomy, 12.","DOI":"10.3390\/agronomy12051055"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Carvajal-Yepes, M., Ospina, J.A., Aranzales, E., Velez-Tobon, M., Correa Abondano, M., Manrique-Carpintero, N.C., and Wenzl, P. (2024). Identifying genetically redundant accessions in the world\u2019s largest cassava collection. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1338377"},{"key":"ref_11","unstructured":"Cock, J.H. (1985). Cassava: New Potential for a Neglected Crop, Westview Press, Inc."},{"key":"ref_12","unstructured":"Food and Agriculture Organization of the United Nations (2025, March 12). FAOSTAT Statistical Database. Available online: http:\/\/www.fao.org\/faostat\/."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1016\/j.cell.2006.12.006","article-title":"The molecular genetics of crop domestication","volume":"127","author":"Doebley","year":"2006","journal-title":"Cell"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Brush, S.B. (2004). Farmers\u2019 Bounty: Locating Crop Diversity in the Contemporary World, Yale University Press.","DOI":"10.12987\/yale\/9780300100495.001.0001"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/BF00051630","article-title":"The origin of Manihot esculenta Crantz (Euphorbiaceae)","volume":"41","author":"Allem","year":"1994","journal-title":"Genet. Resour. Crop Evol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Hillocks, R.J., Thresh, J.M., and Bellotti, A.C. (2002). The origins and taxonomy of cassava. Cassava: Biology, Production, and Utilization, CABI Publishing.","DOI":"10.1079\/9780851995243.0000"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"131","DOI":"10.2307\/2657133","article-title":"Microsatellite variation in cassava (Manihot esculenta, Euphorbiaceae) and its wild relatives: Further evidence for a southern Amazonian origin of domestication","volume":"88","author":"Olsen","year":"2001","journal-title":"Am. J. Bot."},{"key":"ref_18","unstructured":"(2017). Embrapa Mandioca e Fruticultura. Produ\u00e7\u00e3o de Ra\u00edzes e Manivas de Mandioca, Embrapa."},{"key":"ref_19","unstructured":"Food and Agriculture Organization of the United Nations (FAO) (1964). FAO Production Yearbook 1964, FAO."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Hillocks, R.J., and Bellotti, A.C. (2002). Arthropod pests. Cassava: Biology, Production, and Utilization, CABI Publishing.","DOI":"10.1079\/9780851995243.0000"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hillocks, R.J., and Bellotti, A.C. (2002). Bacterial, Fungal and Nematode Diseases. Cassava: Biology, Production, and Utilization, CABI Publishing.","DOI":"10.1079\/9780851995243.0261"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/bs.aivir.2014.10.001","article-title":"Cassava virus diseases: Biology, epidemiology, and management","volume":"91","author":"Legg","year":"2015","journal-title":"Adv. Virus Res."},{"key":"ref_23","unstructured":"FAO\/WHO Joint Expert Committee on Food Additives (1993). Cyanogenic glycosides. Toxicological Evaluation of Certain Food Additives and Naturally Occurring Toxicants (WHO Food Additives Series No. 30; FAO Food and Nutrition Paper 30\/1), World Health Organization. Available online: https:\/\/www.fao.org\/3\/t0207e\/t0207e00.htm."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1080\/03670244.1998.9991546","article-title":"The importance of being bitter: A qualitative study on cassava cultivar preference in Malawi","volume":"37","author":"Mkumbira","year":"1998","journal-title":"Ecol. Food Nutr."},{"key":"ref_25","unstructured":"Wheatley, C.C., and Gomez, G. (1985). Evaluation of Post-Harvest Deterioration in Cassava Roots, Centro Internacional de Agricultura Tropical (CIAT). CIAT Working Document No. 16."},{"key":"ref_26","first-page":"34","article-title":"Classification, cultivation, and selection in Aguaruna cultivars of Manihot esculenta (Euphorbiaceae)","volume":"1","author":"Boster","year":"1984","journal-title":"Adv. Econ. Bot."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1007\/BF02910400","article-title":"Indigenous diversity of cassava: Generation, maintenance, use and loss among the Amuesha, Peruvian Upper Amazon","volume":"51","author":"Salick","year":"1997","journal-title":"Econ. Bot."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1111\/j.1420-9101.2009.01749.x","article-title":"Traditional Amerindian cultivators combine directional and ideotypic selection for sustainable management of cassava genetic diversity","volume":"22","author":"Massol","year":"2009","journal-title":"J. Evol. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1663\/0013-0001(2002)056[0366:GEOCME]2.0.CO;2","article-title":"Germination ecology of cassava (Manihot esculenta Crantz, Euphorbiaceae) in traditional agroecosystems: Seed and seedling biology of a vegetatively propagated domesticated plant","volume":"56","author":"Pujol","year":"2002","journal-title":"Econ. Bot."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Le Thuy, C.T., Lopez-Lavalle, L.A.B., Vu, N.A., Hy, N.H., Nhan, P.T., Ceballos, H., Newby, J., Tung, N.B., Hien, N.T., and Tuan, L.N. (2021). Identifying new resistance to cassava mosaic disease and validating markers for the CMD2 locus. Agriculture, 11.","DOI":"10.3390\/agriculture11090829"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"eadq0018","DOI":"10.1126\/science.adq0018","article-title":"Historic manioc genomes illuminate maintenance of diversity under long-lived clonal cultivation","volume":"387","author":"Kistler","year":"2025","journal-title":"Science"},{"key":"ref_32","unstructured":"Centro Internacional de Agricultura Tropical (CIAT) (1969). Annual Report 1969."},{"key":"ref_33","unstructured":"Centro Internacional de Agricultura Tropical (CIAT) (1970). CIAT Informe Anual 1970, CIAT."},{"key":"ref_34","unstructured":"Gulick, P., Hershey, C., and Esquinas-Alcazar, J.T. (1983). Genetic Resources of Cassava and Wild Relatives, International Board for Plant Genetic Resources (IBPGR)."},{"key":"ref_35","unstructured":"International Plant Genetic Resources Institute (1994). International Network for Cassava Genetic Resources: Report of the First Meeting of the International Network for Cassava Genetic Resources, CIAT, Cali, Colombia, 18\u201323 August 1992, International Plant Genetic Resources Institute. International Crop Network Series No. 10."},{"key":"ref_36","unstructured":"Mejia, G.M. (1991). Diversidad de la Yuca Manihot esculenta Crantz en Colombia. Vision Geographica-Cultural, Corporacion Colombiana para la Amazonia-Araucara. Available online: http:\/\/hdl.handle.net\/20.500.12324\/32432."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1094\/PDIS-10-15-1228-PDN","article-title":"First Report of Sri Lankan cassava mosaic virus Infecting Cassava in Cambodia","volume":"100","author":"Wang","year":"2016","journal-title":"Plant Dis."},{"key":"ref_38","unstructured":"Frison, E.A., and Feliu, E. (1991). FAO\/IBPGR Technical Guidelines for the Safe Movement of Cassava Germplasm, International Board for Plant Genetic Resources."},{"key":"ref_39","unstructured":"Lozano, J.C., and van Schoonhoven, A. (1975). Danger of dissemination of diseases and pests through the introduction of material for the propagation of cassava. International Exchange and Testing of Cassava Germ Plasm, CIAT."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1046\/j.1365-2540.2000.00749.x","article-title":"Assessment of genetic variability in a traditional cassava (Manihot esculenta Crantz) farming system, using AFLP markers","volume":"85","author":"Elias","year":"2000","journal-title":"Heredity"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"792","DOI":"10.3390\/d6040792","article-title":"Differences in manioc diversity among five ethnic groups of the Colombian Amazon","volume":"6","author":"Stomph","year":"2014","journal-title":"Diversity"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1111\/1477-9552.12247","article-title":"Household determinants of the adoption of improved cassava varieties using DNA fingerprinting to identify varieties in farmer fields: A case study in Colombia","volume":"69","author":"Floro","year":"2018","journal-title":"J. Agric. Econ."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s11103-021-01124-0","article-title":"DNA fingerprinting reveals varietal composition of Vietnamese cassava germplasm (Manihot esculenta Crantz) from farmers\u2019 field and genebank collections","volume":"109","author":"Ocampo","year":"2022","journal-title":"Plant Mol. Biol."},{"key":"ref_44","unstructured":"Ortiz, R., and Nassar, N.M.A. (2006). Cassava genetic resources: Wild species and indigenous cultivars and their utilization for breeding of the crop. First International Meeting on Cassava Breeding, Biotechnology and Ecology, Universidade de Brasilia."},{"key":"ref_45","first-page":"1","article-title":"Manihot and Manihotoides (Euphorbiaceae). A computer assisted study","volume":"13","author":"Rogers","year":"1973","journal-title":"Flora Neotrop. Monogr."},{"key":"ref_46","unstructured":"Bertram, R. (1993). Application of Molecular Techniques to Genetic Resources of Cassava (Manihot esculenta Crantz\u2014Euphorbiaceae): Interspecific Evolutionary Relationships and Intraspecific Characterization. [Ph.D. Thesis, University of Maryland]."},{"key":"ref_47","unstructured":"Centro Internacional de Agricultura Tropical (CIAT) (1984). CIAT Annual Report 1984, CIAT."},{"key":"ref_48","unstructured":"Cuervo-Iba\u00f1ez, M., Ni\u00f1o Jimenez, D.P., Aranzales, E., Velez, M., Martinez Vivas, A.M., and Wenzl, P. (2023, January 20\u201325). Phytosanitary evaluation of the in vitro cassava collection (Manihot esculenta Crantz): Collection history and phytosanitary evaluation. Presented at the 12th International Congress of Plant Pathology, Lyon, France."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.virusres.2013.12.011","article-title":"Unraveling complex viral infections in cassava (Manihot esculenta Crantz) from Colombia","volume":"186","author":"Olaya","year":"2014","journal-title":"Virus Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"29648","DOI":"10.1038\/s41598-024-81142-2","article-title":"Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas","volume":"14","author":"Jimenez","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_51","first-page":"77","article-title":"Detection and identification of \u201cCandidatus Phytoplasma asteris\u201d-related phytoplasmas associated with a witches\u2019 broom disease of cassava in Vietnam","volume":"3","author":"Pardo","year":"2013","journal-title":"Phytopathogenic Mollicutes"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"22500","DOI":"10.1038\/s41598-023-49735-5","article-title":"Ceratobasidium sp. is associated with cassava witches\u2019 broom disease, a re-emerging threat to cassava cultivation in Southeast Asia","volume":"13","author":"Leiva","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_53","unstructured":"Mafla, G., and Debouck, D.G. (2004). Biodiversity of Cassava: Challenges and innovations for its conservation. Proceedings of the Invited Presentation at Cassava Biotechnology Network (CBN-VI), 12 March 2004, Palmira, Colombia, International Center for Tropical Agriculture (CIAT)."},{"key":"ref_54","unstructured":"Ospina, P.B., and Hern\u00e1n, C. (2012). Manihot genetic resources at CIAT (Centro Internacional de Agricultura Tropical). Cassava in the Third Millennium: Modern Production, Processing, Use and Marketing Systems, CIAT."},{"key":"ref_55","unstructured":"Centro Internacional de Agricultura Tropical (CIAT) (2003). Annual Report 2003\u2014Project PE-1, Integrated Pest and Disease Management in Major Agroecosystems, CIAT. Cassava Program."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1094\/Phyto-65-826","article-title":"Elimination of cassava mosaic disease by meristem culture","volume":"65","author":"Kartha","year":"1975","journal-title":"J. Phytopathol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/0304-4211(74)90066-2","article-title":"Regeneration of cassava plants from apical meristems","volume":"2","author":"Kartha","year":"1974","journal-title":"Plant Sci. Lett."},{"key":"ref_58","unstructured":"Mafla, G., Roa, J.C., Aranzales, E., and Debouck, D.G. (2010). Handbook of Procedures for In Vitro Germplasm Conservation of the Genus Manihot; Genetic Resources Program, Centro Internacional de Agricultura Tropical (CIAT)."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","article-title":"A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures","volume":"15","author":"Murashige","year":"1962","journal-title":"Physiol. Plant."},{"key":"ref_60","unstructured":"Carvalho, L., Thro, A.M., and Duarte, A. (2000). Advances on the in vitro growth control of Cassava, using silver nitrate. Proceedings of the Cassava Biotechnology: International Scientific Meeting-CBN (IV, 1998, Salvador, Bahia, Brazil), EMBRAPA-Recursos Gen\u00e9ticos e Biotecnolog\u00eda."},{"key":"ref_61","unstructured":"Roca, W.M., and Mroginski, L.A. (1991). Eliminaci\u00f3n de virus y propagaci\u00f3n de clones en la yuca (Manihot esculenta Crantz). Fundamentos y Aplicaciones, Centro Internacional de Agricultura Tropical (CIAT)."},{"key":"ref_62","unstructured":"CIAT (1982). El cultivo de meristemas para el saneamiento de clones de yuca. Gu\u00eda de Estudio, Centro Internacional de Agricultura Tropical."},{"key":"ref_63","unstructured":"Vel\u00e1squez, E., and Mafla, G. (1999). Conservaci\u00f3n in vitro: Una alternativa segura para preservar especies silvestres de Manihot spp. (Euphorbiaceae). Congreso Nacional de Conservaci\u00f3n 54 de la Biodiversidad (2, 1999, Bogot\u00e1, Colombia), Pontificia Universidad Javeriana. Memorias."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"227","DOI":"10.17660\/ActaHortic.2014.1039.29","article-title":"Using a droplet-vitrification method to partially overcome the recalcitrance of cassava to cryostorage","volume":"1039","author":"Escobar","year":"2014","journal-title":"Acta Hortic."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Panis, B.M., and Van den Houwe, I. (2020). Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks in In vitro Collections and\/or in Liquid Nitrogen. Plants, 9.","DOI":"10.3390\/plants9121634"},{"key":"ref_66","unstructured":"Escobar, R.H. (2005). Aspectos Log\u00edsticos de Manejo y Determinaci\u00f3n de la Estabilidad Gen\u00e9tica de Materiales Crioconservados de Yuca (Manihot esculenta Crantz). [Master\u2019s Thesis, Universidad Nacional de Colombia, Sede Palmira]."},{"key":"ref_67","unstructured":"Acker, J.P., Adkins, S., Alves, A., Horna, D., and Toll, J. (2017). Feasibility study for a safety back-up cryopreservation facility. Independent Expert Report: July 2017, Bioversity International."},{"key":"ref_68","unstructured":"Centro Internacional de Agricultura Tropical (CIAT) (2002). CIAT Project on Saving Biodiversity SB-01: Annual Report 2002 Genetic Resources Unit Report on Achievements and Progresses, International Center for Tropical Agriculture (CIAT)."},{"key":"ref_69","unstructured":"Ni\u00f1o-Jimenez, D.P., Aranzales, E., Erazo, J.C., L\u00f3pez, J., and V\u00e9lez, M. (2016). The Bonsai as an alternative safety duplication system of the world cassava collection preserved at CIAT. Poster Presentado en el II Simposio de Recursos Fitogen\u00e9ticos Neotropicales, 3 al 5 de Noviembre del 2.016. Universidad Nacional de Colombia sede Palmira. Valle del Cauca. Colombia, Centro Internacional de Agricultura Tropical (CIAT)."},{"key":"ref_70","unstructured":"International Institute of Tropical Agriculture (IITA) (1979). Cassava Program Annual Report 1979, IITA."},{"key":"ref_71","first-page":"257","article-title":"Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen","volume":"11","author":"Marin","year":"1990","journal-title":"Cryo-Letters"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1093\/oxfordjournals.aob.a085447","article-title":"Germination and Liquid Nitrogen Storage of Cassava Seed","volume":"42","author":"Mumford","year":"1978","journal-title":"Ann. Bot."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Pineda, M., Yu, B., Tian, Y., Morante, N., Salazar, S., Hyde, P., Setter, T., and Ceballos, H. (2020). Effect of Pruning Young Branches on Fruit and Seed Set in Cassava. Front. Plant Sci. Sec. Plant Breed., 11.","DOI":"10.3389\/fpls.2020.01107"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Baguma, J.K., Mukasa, S.B., Nuwamanya, E., Alicai, T., Omongo, C., Hyde, P.T., Setter, T.L., Ochwo-Ssemakula, M., Esuma, W., and Kanaabi, M. (2023). Flowering and fruit-set in cassava under extended red-light photoperiod supplemented with plant-growth regulators and pruning. BMC Plant Biol., 23.","DOI":"10.1186\/s12870-023-04349-x"},{"key":"ref_75","unstructured":"Ch\u00e1vez, R., Roca, W.M., Arias Garz\u00f3n, D., Withers, L.A., and Williams, T. (1988). Establishment and Operation of a Pilot In Vitro Active Genebank of Cassava, Centro Internacional de Agricultura Tropical (CIAT)."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/BF00027481","article-title":"Stability of cassava plants at the DNA level after retrieval from 10 years of in vitro storage","volume":"90","author":"Angel","year":"1996","journal-title":"Euphytica"},{"key":"ref_77","unstructured":"Mafla, G., Roa, J.C., Ocampo, C.H., Gallego, G., Jaramillo, G., and Debouck, D.G. (2004). Efficacy of silver nitrate for slow growth conservation of cassava (Manihot esculenta Crantz). Determination of Viability and Genetic Stability. Proceedings of the Sixth International Scientific Meeting of the Cassava Biotechnology Network, Cali, Colombia, 8\u201314 March 2004, CIAT."},{"key":"ref_78","first-page":"1","article-title":"New marker genes found in cassava","volume":"13","author":"Hershey","year":"1989","journal-title":"Cassava Newsl."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1590\/S0006-87051942000100002","article-title":"Gen\u00e9tica de Manihot: I. hereditariedade da forma da folha e da colora\u00e7\u00e3o da pel\u00edcula externa das raizes em Manihot Utilissima pohl","volume":"2","author":"Graner","year":"1942","journal-title":"Bragantia"},{"key":"ref_80","first-page":"494","article-title":"Genetics of male sterility in a genotype of cassava","volume":"53","author":"Jos","year":"1984","journal-title":"Curr. Sci."},{"key":"ref_81","unstructured":"Roca, W., and Thro, A.M. (1992). Esterase isozyme fingerprinting of the cassava germplasm collection held at CIAT. First International Scientific Meeting Cassava Biotechnology Network. Cartagena de Indias, CIAT."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1023\/A:1009627231450","article-title":"Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-based markers to maintain germplasm collections","volume":"5","author":"Maya","year":"1999","journal-title":"Mol. Breed."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"2672","DOI":"10.1093\/plphys\/kiad269","article-title":"Integrated genetic and metabolic characterization of Latin American cassava (Manihot esculenta) germplasm","volume":"192","author":"Ovalle","year":"2023","journal-title":"Plant Physiol."},{"key":"ref_84","first-page":"40","article-title":"Genetic diversity and phylogeny of cassava (Manihot esculenta Crantz) as revealed by RFLP analysis","volume":"40","author":"Fregene","year":"1997","journal-title":"Genome"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Frediansyah, A. (2021). Identification of Cassava Varieties in Ex-Situ Collections and Global Farmer\u2019s Fields: An Update from 1990 to 2020. Cassava\u2014Biology, Production and Use, IntechOpen.","DOI":"10.5772\/intechopen.87488"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1007\/s00122-005-1937-4","article-title":"DArT for high-throughput genotyping of cassava (Manihot esculenta) and its wild relatives","volume":"110","author":"Xia","year":"2005","journal-title":"Theor. Appl. Genet."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s12042-011-9087-0","article-title":"Molecular markers and their application to cassava breeding: Past, present and future","volume":"5","author":"Ferguson","year":"2012","journal-title":"Trop. Plant Biol."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Ferguson, M.E., Shah, T., Kulakow, P., and Ceballos, H. (2019). A global overview of cassava genetic diversity. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0224763"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Amelework, A.B., and Bairu, M.W. (2022). Advances in Genetic Analysis and Breeding of Cassava (Manihot esculenta Crantz): A Review. Plants, 11.","DOI":"10.3390\/plants11121617"},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Hershey, C.E. (2017). Molecular approaches in cassava breeding. Achieving Sustainable Cultivation of Cassava Volume 2 Cultivation Techniques, Burleigh Dodds Science Publishing Limited.","DOI":"10.4324\/9781351114318"},{"key":"ref_91","unstructured":"Amundson, K.R. (2021). Haploid Induction and Somatic Mutations of Potato. [Doctoral Dissertation, University of California]. Available online: https:\/\/escholarship.org\/uc\/item\/8nk358tm."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s00122-025-05030-7","article-title":"Resolving a century-old enigma: Potato \u2018Bolters\u2019 originate from instability of the StCDF1.3 allele","volume":"138","author":"Clot","year":"2025","journal-title":"Theor. Appl. Genet."},{"key":"ref_93","unstructured":"Arber, W.K., Llimensee, K., Peacock, W.J., and Stralinger, P. (1984). Genetic Perspectives of Germplasm Conservation. Genetic Manipulation: Impact on Man and Society, Cambridge University Press."},{"key":"ref_94","unstructured":"Hershey, C.H., Iglesias, C., Iwanaga, M., and Tohme, J. (1994). Definition of a core collection for cassava. Meeting of the International Network for Cassava Genetic Resources (1, 1992, Cali, Colombia). International Network for Cassava Genetic Resources: Report, International Plant Genetic Resources Institute (IPGRI). International crop network series 10."},{"key":"ref_95","unstructured":"Centro Internacional de Agricultura Tropical (CIAT) (1993). CIAT Cassava Program Report, 1987\u20131989: Cassava Utilization, 1989; CIAT Working Document No. 91, CIAT."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1663\/0013-0001(2004)058[0242:GDOTSA]2.0.CO;2","article-title":"Genetic diversity of traditional South American landraces of cassava (Manihot esculenta Crantz): An analysis using microsatellites","volume":"58","author":"Elias","year":"2004","journal-title":"Econ. Bot."},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"dos Santos, C.C., de Andrade, L.R.B., dos Carmo, C.D., and de Oliveira, E.J. (2023). Development of cassava core collections based on morphological and agronomic traits and SNPS markers. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1250205"},{"key":"ref_98","unstructured":"Hershey, C.H. (2020). Cassava Genetic Improvement: Theory and Practice, International Center for Tropical Agriculture."},{"key":"ref_99","unstructured":"Food and Agriculture Organization (2006). Resolution 1\/2006: Adoption of the Standard Material Transfer Agreement, International Treaty on Plant Genetic Resources for Food and Agriculture."},{"key":"ref_100","unstructured":"Engelmann, F., and Takagi, H. (2000). Cryopreservation of germplasm for conservation of genetic resources. Cryopreservation of Tropical Plant Germplasm: Current Research Progress and Applicatio, IPGRI."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1007\/s00122-015-2555-4","article-title":"Conventional breeding, marker-assisted selection, genomic selection and inbreeding in clonally propagated crops: A case study for cassava","volume":"128","author":"Ceballos","year":"2015","journal-title":"Theor. Appl. Genet."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/13\/1981\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T04:18:34Z","timestamp":1783570714000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/13\/1981"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,26]]},"references-count":101,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["plants15131981"],"URL":"https:\/\/doi.org\/10.3390\/plants15131981","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,26]]}}}