{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:09:56Z","timestamp":1785431396714,"version":"3.56.0"},"reference-count":50,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technological Development and Innovation of Republic of Serbia","award":["451-03-33\/2026-03\/200134"],"award-info":[{"award-number":["451-03-33\/2026-03\/200134"]}]},{"award":["451-03-33\/2026-03\/200134"],"award-info":[{"award-number":["451-03-33\/2026-03\/200134"]}],"id":[{"id":"https:\/\/ror.org\/01znas443","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Alchemilla subcrenata Buser is a montane Alchemilla (Rosaceae) species with established traditional medicinal use but only limited prior phytochemical characterization, restricted to preliminary observations on phenolic content and peroxidase activity. This study reports the first systematic optimisation of microwave-assisted extraction (MAE) conditions for this species using a Box\u2013Behnken design within a Response Surface Methodology (RSM) framework. Three extraction variables, ethanol concentration (30\u201370%, v\/v), solid-to-liquid ratio (1:10\u20131:20, w\/v), and extraction time (10\u201330 min), were simultaneously optimised against twelve response variables encompassing total phenolic and flavonoid contents, six antioxidant assays, and four enzyme inhibitory activities (acetylcholinesterase, butyrylcholinesterase, tyrosinase, and \u03b1-amylase). Second-order polynomial models demonstrated good predictive ability for most responses (R2 = 0.878\u20130.971 for eleven of the twelve responses), with the exception of metal chelation, which showed a lower fit (R2 = 0.671) and was excluded from optimisation; the multiobjective optimization identified optimal conditions of 60% ethanol, 1:12.5 (w\/v) solid-to-liquid ratio, and 10 min extraction time. Under optimal conditions, MAE delivered exceptional in vitro antioxidant and enzyme inhibitory activities from A. subcrenata. LC-MS\/MS characterisation, representing the first comprehensive phenolic profiling of this species, identified 28 phenolic compounds (1599.7 \u03bcg\/g extract), dominated by quercetin-3-O-hexoside, gallic acid, catechin, and kaempferol glycosides; naringenin and aesculetin represent novel phytochemical records for A. subcrenata. The abundant presence of quercetin hexosides and hydroxycinnamic acids offers a mechanistic explanation for the broad, multi-target bioactivity observed, positioning A. subcrenata as a promising source of bioactive phenolics that warrants further investigation for potential nutraceutical, pharmaceutical, and cosmeceutical applications.<\/jats:p>","DOI":"10.3390\/plants15142161","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:55:21Z","timestamp":1783990521000},"page":"2161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unlocking the Bioactive Power of Alchemilla subcrenata Buser via Optimized Green Extraction"],"prefix":"10.3390","volume":"15","author":[{"given":"Jelena","family":"Vukovi\u0107","sequence":"first","affiliation":[{"name":"Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8039-1061","authenticated-orcid":false,"given":"Jelena","family":"Bajac","sequence":"additional","affiliation":[{"name":"Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6548-7823","authenticated-orcid":false,"given":"Gokhan","family":"Zengin","sequence":"additional","affiliation":[{"name":"Science Faculty, Selcuk University, Campus Konya, Konya 42130, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2384-5546","authenticated-orcid":false,"given":"Tatjana","family":"Majki\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovi\u0107a 3, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9542-9576","authenticated-orcid":false,"given":"Lidija","family":"Petrovi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7192-4962","authenticated-orcid":false,"given":"Milena","family":"Terzi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Technology Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"117439","DOI":"10.1016\/j.jep.2023.117439","article-title":"A review of the traditional uses, phytochemistry, pharmacology and clinical evidence for the use of the genus Alchemilla (Rosaceae)","volume":"320","author":"Jakimiuk","year":"2024","journal-title":"J. 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