{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:29:07Z","timestamp":1790051347958,"version":"4.0.1"},"reference-count":119,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015690","name":"Universitas Padjadjaran","doi-asserted-by":"crossref","award":["2097\/UN6.0\/TU.00\/2025"],"award-info":[{"award-number":["2097\/UN6.0\/TU.00\/2025"]}],"id":[{"id":"10.13039\/501100015690","id-type":"DOI","asserted-by":"crossref"}]},{"award":["2097\/UN6.0\/TU.00\/2025"],"award-info":[{"award-number":["2097\/UN6.0\/TU.00\/2025"]}],"id":[{"id":"https:\/\/ror.org\/00xqf8t64","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Natural products remain a rich source of chemically diverse molecules with therapeutic potential, and Premna serratifolia represents one such promising botanical resource. This review provides a comprehensive overview of its bioactive constituents by integrating published evidence related to extraction variability, chemical profiling, physicochemical screening, and pharmacological activities. Available studies indicate that differences in extraction techniques influence the distribution of secondary metabolites, particularly phenolic and flavonoid compounds associated with biological activity. Previous phytochemical investigations have reported a structurally diverse set of compounds spanning multiple chemical classes. Evaluations based on drug-relevant parameters suggest that several constituents possess favorable structural and physicochemical characteristics. Published studies further suggest that selected compounds may interact with multiple protein targets involved in disease-related pathways. Computational evidence reported in the literature highlights potential molecular interactions and signaling networks associated with therapeutic activities. These pathways have been linked to biological processes such as cellular regulation, oxidative balance, and inflammatory responses. Available experimental evidence indicates that several compounds exhibit noteworthy bioactivities across different biological systems. Structural features, including functional group distribution and degree of substitution, have been reported to influence molecular interactions and pharmacokinetic properties. Simpler molecular frameworks often appear to show greater compatibility with drug-likeness criteria. The integration of phytochemical, pharmacological, and computational evidence provides a useful framework for compound prioritization and evaluation. Such an approach may facilitate the transition from phytochemical discovery toward pharmacological development. Further studies involving structural optimization, advanced computational modeling, and experimental validation are warranted to better characterize the therapeutic potential of these compounds. The available evidence suggests that Premna serratifolia is a valuable source of bioactive molecular scaffolds with considerable potential for future multitarget drug discovery and therapeutic development.<\/jats:p>","DOI":"10.3390\/plants15142176","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T01:05:07Z","timestamp":1784163907000},"page":"2176","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Integrated Phytochemical and Pharmacological Insights into Premna serratifolia for Drug Discovery: A Narrative Review"],"prefix":"10.3390","volume":"15","author":[{"given":"Alfred","family":"Ariyanto","sequence":"first","affiliation":[{"name":"Doctoral Program of Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang KM 21, Sumedang 45363, Indonesia"},{"name":"Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jalan Raya Bandung-Smeeding KM 21, Sumedang 45363, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3682-244X","authenticated-orcid":false,"given":"Wisnu Ananta","family":"Kusuma","sequence":"additional","affiliation":[{"name":"School of Data Science, Mathematics, and Informatics, IPB University, Jalan Raya Dramaga, Bogor 16680, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6269-5931","authenticated-orcid":false,"given":"Muhaimin","family":"Muhaimin","sequence":"additional","affiliation":[{"name":"Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang KM 21, Jatinangor 45363, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezatul Ezleen","family":"Kamarulzaman","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Universiti Sains Malaysia, Jalan Sungai Dua, Minden 11800, Penang, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7155-4412","authenticated-orcid":false,"given":"Taufik Muhammad","family":"Fakih","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jalan Raya Bandung-Smeeding KM 21, Sumedang 45363, Indonesia"},{"name":"Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Bandung, Jalan Ranggagading, Bandung 40116, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6156-8025","authenticated-orcid":false,"given":"Muchtaridi","family":"Muchtaridi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jalan Raya Bandung-Smeeding KM 21, Sumedang 45363, Indonesia"},{"name":"Research Collaboration Centre for Radiopharmaceuticals Theranostics, National Research and Innovation Agency (BRIN), Jalan Raya Bandung-Sumedang KM 21, Sumedang 45363, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3436940","DOI":"10.1155\/2020\/3436940","article-title":"Antioxidant Activity, Enzyme Inhibition Potentials, and Phytochemical Profiling of Premna serratifolia L. Leaf Extracts","volume":"2020","author":"Simamora","year":"2020","journal-title":"Int. J. Food Sci."},{"key":"ref_2","unstructured":"Tharakan, S.T. (2021). Phytochemical screening and in vitro antioxidant activity of Premna serratifolia L. leaf extracts. Int. J. Food Nutr. Sci., 10."},{"key":"ref_3","unstructured":"Soepadmo, E., Saw, L.G., and Chung, R.C.K. (2000). Tree Flora of Sabah and Sarawak, Ampang Press Sdn. Bhd.. Available online: https:\/\/books.google.com.my\/books?id=M9UsAQAAMAAJ&q=tree+flora+of+sabah+and+sarawak+volume+5&dq=tree+flora+of+sabah+and+sarawak+volume+5&hl=en&sa=X&ved=0ahUKEwjyk9vEg-vZAhUEspQKHexKBGsQ6AEIKDAA."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"233","DOI":"10.22271\/phyto.2025.v14.i5d.15576","article-title":"A review of the pharmacological evaluation of Premna Serratifolia","volume":"14","author":"Sajeena","year":"2025","journal-title":"J. Pharmacogn. Phytochem."},{"key":"ref_5","first-page":"426","article-title":"Pharmacognostical Standardization on the Roots of Agnimantha-Premna serratifolia Linn","volume":"5","author":"Reddy","year":"2018","journal-title":"Int. J. Pharmacogn."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1080\/13880209.2017.1323225","article-title":"Ethnomedicinal uses, phytochemistry and pharmacological aspects of the genus Premna: A review","volume":"55","author":"Dianita","year":"2017","journal-title":"Pharm. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.2147\/CMAR.S516204","article-title":"Anticancer Potential of Bioactive Compounds in Premna serratifolia, Premna odorata, and Premna tomentosa: A Review of In Vitro Evidence","volume":"17","author":"Febriyanti","year":"2025","journal-title":"Cancer Manag. Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Bhagwat, S.K., Patil, S.V., Vidal-Limon, A., Jimenez-Halla, J.O.C., Ghotekar, B.K., Bobade, V.D., P\u00e9rez-Landa, I.D., Delgado-Alvarado, E., Hern\u00e1ndez-Rosas, F., and Pawar, T.J. (2025). Design and Evaluation of Indole-Based Schiff Bases as \u03b1-Glucosidase Inhibitors: CNN-Enhanced Docking, MD Simulations, ADMET Profiling, and SAR Analysis. Molecules, 30.","DOI":"10.20944\/preprints202508.0415.v1"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1039\/c1np00051a","article-title":"Multitargeting by curcumin as revealed by molecular interaction studies","volume":"28","author":"Gupta","year":"2011","journal-title":"Nat. Prod. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5109","DOI":"10.1007\/s11101-025-10066-0","article-title":"Ecological and environmental determinants of phytochemical variability in forest trees","volume":"24","author":"Ogwu","year":"2025","journal-title":"Phytochem. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e33917","DOI":"10.1016\/j.heliyon.2024.e33917","article-title":"Recent advances in identifying protein targets of bioactive natural products","volume":"10","author":"Jiang","year":"2024","journal-title":"Heliyon"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1177\/1087057114530783","article-title":"Automated structure-activity relationship mining: Connecting chemical structure to biological profiles","volume":"19","author":"Wawer","year":"2014","journal-title":"J. Biomol. Screen."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"178","DOI":"10.24198\/ijbp.v2i3.43537","article-title":"Phytochemistry and Pharmacological Potentials of Premna serratifolia L.: Traditional Medicinal Plant Used by Local People in Kalimantan","volume":"2","author":"Khairunnisa","year":"2022","journal-title":"Indones. J. Biol. Pharm."},{"key":"ref_14","first-page":"101","article-title":"Anti-arthritic activity of Premna serratifolia Linn., wood against adjuvant induced arthritis","volume":"2","author":"Rajendran","year":"2010","journal-title":"Avicenna J. Med. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Elmaidomy, A.H., Alhadrami, H.A., Amin, E., Aly, H.F., Othman, A.M., Rateb, M.E., Hetta, M.H., Abdelmohsen, U.R., and Hassan, H.M. (2020). Anti-inflammatory and antioxidant activities of terpene- And polyphenol-rich premna odorata leaves on alcohol-inflamed femalewistar albino rat liver. Molecules, 25.","DOI":"10.3390\/molecules25143116"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s13205-021-02654-6","article-title":"Phytochemical profiling and cytotoxic evaluation of Premna serratifolia L. against human liver cancer cell line","volume":"11","author":"Singh","year":"2021","journal-title":"3 Biotech"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3329\/jsr.v1i1.1046","article-title":"Evaluation of Hepatoprotective and In-vitro Cytotoxic Activity of Leaves of Premna serratifolia Linn","volume":"1","author":"Vadivu","year":"2008","journal-title":"J. Sci. Res."},{"key":"ref_18","first-page":"e01585","article-title":"A review of modern and conventional extraction techniques and their applications for extracting phytochemicals from plants","volume":"19","author":"Bitwell","year":"2023","journal-title":"Sci. Afr."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Sun, S., Yu, Y., Jo, Y., Han, J.H., Xue, Y., Cho, M., Bae, S.J., Ryu, D., Park, W., and Ha, K.T. (2025). Impact of extraction techniques on phytochemical composition and bioactivity of natural product mixtures. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1615338"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.soilbio.2014.04.011","article-title":"Temperature and duration of extraction affect the biochemical composition of soil water-extractable organic matter","volume":"75","author":"Chantigny","year":"2014","journal-title":"Soil Biol. Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"111779","DOI":"10.1016\/j.microc.2024.111779","article-title":"Metabolic profiling of plant and other natural products: Platforms, applications and quality evaluation","volume":"207","author":"Adeeyo","year":"2024","journal-title":"Microchem. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"100676","DOI":"10.1016\/j.cjac.2025.100676","article-title":"Targeting Key Breast Cancer Regulators with Citrus reticulata-Derived Compounds: Insights from Network Pharmacology and Molecular Dynamics","volume":"54","author":"Fakih","year":"2025","journal-title":"Chin. J. Anal. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Noor, F., Qamar, M.T.U., Ashfaq, U.A., Albutti, A., Alwashmi, A.S.S., and Aljasir, M.A. (2022). Network Pharmacology Approach for Medicinal Plants: Review and Assessment. Pharmaceuticals, 15.","DOI":"10.3390\/ph15050572"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"10","DOI":"10.5962\/p.373659","article-title":"Australian Tropical Rainforest Plants Identification System","volume":"25","author":"Zich","year":"2016","journal-title":"Australas. Plant Conserv. J. Aust. Netw. Plant Conserv."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Salem, M.A., De Souza, L.P., Serag, A., Fernie, A.R., Farag, M.A., Ezzat, S.M., and Alseekh, S. (2020). Metabolomics in the context of plant natural products research: From sample preparation to metabolite analysis. Metabolites, 10.","DOI":"10.3390\/metabo10010037"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100193","DOI":"10.1016\/j.insi.2026.100193","article-title":"Artificial Intelligence and In Silico Study Using De Novo Drug Design to Explore Molecular Targets of Burkholderia pseudomallei for Melioidosis Treatment","volume":"2","author":"Lopes","year":"2026","journal-title":"Silico Res. Biomed."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2694","DOI":"10.3762\/bjoc.12.267","article-title":"Computational methods in drug discovery","volume":"12","author":"Leelananda","year":"2016","journal-title":"Beilstein J. Org. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s10822-025-00621-4","article-title":"A computational study of cardiac glycosides from Vernonia amygdalina as PI3K inhibitors for targeting HER2 positive breast cancer","volume":"39","author":"Tafrihani","year":"2025","journal-title":"J. Comput. Aided. Mol. Des."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"100680","DOI":"10.1016\/j.phyplu.2024.100680","article-title":"Phytochemical and toxicological evaluation of aqueous leaf extract of Premna integrifolia L. in male balb\/c mice","volume":"5","author":"Suman","year":"2025","journal-title":"Phytomed. Plus"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"132","DOI":"10.4103\/0257-7941.179864","article-title":"Pharmacological potentials of Premna integrifolia L","volume":"35","author":"Mali","year":"2016","journal-title":"Anc. Sci. Life"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Bibi, N., Shah, M.H., Khan, N., Al-Hashimi, A., Elshikh, M.S., Iqbal, A., Ahmad, S., and Abbasi, A.M. (2022). Variations in Total Phenolic, Total Flavonoid Contents, and Free Radicals\u2019 Scavenging Potential of Onion Varieties Planted under Diverse Environmental Conditions. Plants, 11.","DOI":"10.3390\/plants11070950"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"16721","DOI":"10.1007\/s13399-024-06368-6","article-title":"Optimizing extraction methods: The role of solvent polarity in enhancing phenolic content and antioxidant activity in biowaste","volume":"15","author":"Mamoona","year":"2025","journal-title":"Biomass Convers. Biorefinery"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"100169","DOI":"10.1016\/j.nxnano.2025.100169","article-title":"Synthesis of nanoparticles using advanced techniques","volume":"8","author":"Vinukonda","year":"2025","journal-title":"Next Nanotechnol."},{"key":"ref_34","first-page":"e01594","article-title":"A review on the trends of endophytic fungi bioactivities","volume":"20","author":"Omomowo","year":"2023","journal-title":"Sci. Afr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1007\/s10068-025-01930-y","article-title":"Fermentation-induced modifications to the structural, surface, and functional properties of quinoa proteins","volume":"34","author":"Alrosan","year":"2025","journal-title":"Food Sci. Biotechnol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"153","DOI":"10.4308\/hjb.33.1.153-166","article-title":"Anti-glucosidase Activity and Antioxidant Capacity of Premna serratifolia from Sintang, Kapuas Hulu, and Sambas District, West Kalimantan, Indonesia","volume":"33","author":"Hadiarti","year":"2026","journal-title":"HAYATI J. Biosci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1656","DOI":"10.5530\/pj.2021.13.213","article-title":"Optimization of Solvent Extraction of the Leaves of Premna serratifolia and Quantitation of Premnaodoroside A by Validated TLC-densitometric Method","volume":"13","author":"Yee","year":"2021","journal-title":"Pharmacogn. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.5530\/pj.2018.6.189","article-title":"Chemical characteristics and in vitro antidiabetic and antioxidant activities of Premna serratifolia L. leaf infusion and decoction","volume":"10","author":"Timotius","year":"2018","journal-title":"Pharmacogn. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.22207\/JPAM.18.2.32","article-title":"In vitro Biomedical Application of Endophytic Aspergillus melleus Isolated from Leaves of Premna serratifolia L","volume":"18","author":"Varsha","year":"2024","journal-title":"J. Pure Appl. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1007\/s13204-014-0397-z","article-title":"Biosynthesis of silver nanoparticles from Premna serratifolia L. leaf and its anticancer activity in CCl4-induced hepato-cancerous Swiss albino mice","volume":"5","author":"Karmegam","year":"2015","journal-title":"Appl. Nanosci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"93","DOI":"10.22146\/mot.71033","article-title":"Physical Stability and Antioxidant Activity of Peel-Off Gel Mask Ethanol Extract of Buas-buas Leaf (Premna serratifolia L.)","volume":"27","author":"Puspita","year":"2022","journal-title":"Maj. Obat Tradis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.31788\/RJC.2021.1426320","article-title":"Understanding phytochemical roles on \u03b1-glucosidase inhibitory activity based on metabolomic approach of Premna serratifolia leaves from west borneo, indonesia","volume":"14","author":"Hadiarti","year":"2021","journal-title":"Rasayan J. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"106976","DOI":"10.1016\/j.surfin.2025.106976","article-title":"Green in situ fabrication of silver nanoparticles coated silk using aqueous leaf extract of Premna serratifolia (L.) for antibacterial textiles","volume":"72","author":"Nguyen","year":"2025","journal-title":"Surf. Interfaces"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"43","DOI":"10.4038\/jnsfsr.v44i1.7980","article-title":"Antimicrobial constituents of Hypocrea virens, an endophyte of the mangrove-associate plant Premna serratifolia L","volume":"44","author":"Ratnaweera","year":"2016","journal-title":"J. Natl. Sci. Found. Sri Lanka"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1080\/10412905.1998.9700905","article-title":"Volatile constituents of the flower buds concrete of Premna serratifolia L","volume":"10","author":"Teai","year":"1998","journal-title":"J. Essent. Oil Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"62","DOI":"10.22146\/mot.86919","article-title":"Formulation and Sunscreen Activity Test of Lotion of Buas-Buas (Premna serratifolia Linn.) Leaf Extract","volume":"29","author":"Adahane","year":"2024","journal-title":"Maj. Obat Tradis."},{"key":"ref_47","first-page":"89","article-title":"Profiling of \u03b1-glucosidase inhibitors from ethyl acetate fraction of Buas-buas (Premna serratifolia) leaves using UHPLC-Q-Orbitrap HRMS and protein\u2013ligand interaction with molecular docking","volume":"13","author":"Hadiarti","year":"2023","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_48","first-page":"164","article-title":"Isnindar Comparing Antioxidant Activity of Extracts and Gel Preparations Combination of Buas-buas leaves (Premna serratifolia L.) and Secang Wood (Caesalpinia sappan)","volume":"28","author":"Tarung","year":"2023","journal-title":"Maj. Obat Tradis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"481","DOI":"10.29090\/psa.2021.05.20.055","article-title":"Variation in chemical constituents of essential oils of the fresh, dried and fermented leaves of Premna serratifolia","volume":"48","author":"Yee","year":"2021","journal-title":"Pharm. Sci. Asia"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.fitote.2018.12.008","article-title":"Lignans with melanogenesis effects from Premna serratifolia wood","volume":"133","author":"Woo","year":"2019","journal-title":"Fitoterapia"},{"key":"ref_51","first-page":"113","article-title":"A new tetrahydrofuran lignan from Premna serratifolia wood","volume":"14","author":"Woo","year":"2019","journal-title":"Nat. Prod. Commun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1080\/13880209.2017.1301491","article-title":"Bioactivity guided isolation of cytotoxic terpenoids and steroids from Premna serratifolia","volume":"55","author":"Biradi","year":"2017","journal-title":"Pharm. Biol."},{"key":"ref_53","first-page":"605","article-title":"Influence of light intensity and temperature on antioxidant activity in Premna serratifolia L","volume":"15","author":"Chua","year":"2015","journal-title":"J. Soil Sci. Plant Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"205","DOI":"10.3329\/bjp.v10i1.21658","article-title":"Cytotoxic activity of isolated constituents from leaves of Premna serratifolia on MCF-7 and HT-29 cell lines","volume":"10","author":"Biradi","year":"2015","journal-title":"Bangladesh J. Pharmacol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1002\/ptr.5229","article-title":"A novel diterpene skeleton: Identification of a highly aromatic, cytotoxic and antioxidant 5-methyl-10-demethyl-abietane-type diterpene from Premna serratifolia","volume":"29","author":"Habtemariam","year":"2015","journal-title":"Phyther. Res."},{"key":"ref_56","first-page":"1","article-title":"Antioxidant Activity of Extract and Fractions of Bebuas Leaves (Premna serratifolia L.) using DPPH Method","volume":"4","author":"Rafif","year":"2024","journal-title":"Indones. J. Biol. Pharm."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Wulandari, I.R., and Luliana, S. (2021). Skrining fitokimia dari ekstrak etanol daus buas-buas (Premna serratifolia Linn.) dan Kayu secang (Caesalpinia sappan Linn.). Media Farm., 18.","DOI":"10.31001\/jfi.v18i2.954"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"264","DOI":"10.13005\/bpj\/3351","article-title":"LC-MS Based Metabolomics, In vitro and In silico Approaches to Characterize Possible Inhibitors of Alpha-glucosidase and Protein Glycation from Premna serratifolia L: Implications in Controlling Diabetes","volume":"1","author":"Dangi","year":"2026","journal-title":"Biomed. Pharmacol. J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.52711\/0974-360X.2025.00144","article-title":"Masriani Fingerprinting, Breast Cancer Inhibition of Premna serratifolia Extracts by In vitro and In silico Approaches","volume":"18","author":"Hadiarti","year":"2025","journal-title":"Res. J. Pharm. Technol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e70024","DOI":"10.1002\/ansa.70024","article-title":"Recent Advances in Liquid Chromatography\u2013Mass Spectrometry (LC\u2013MS) Applications in Biological and Applied Sciences","volume":"6","author":"Alanazi","year":"2025","journal-title":"Anal. Sci. Adv."},{"key":"ref_61","first-page":"401","article-title":"LC-MS\/MS-guided profiling and network pharmacology analysis of bioactive compounds from Costus speciosus targeting type 2 diabetes: Insights from molecular docking and dynamics","volume":"3","author":"Fakih","year":"2025","journal-title":"Intell. Pharm."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1038\/s41467-024-47693-8","article-title":"Exposing the molecular heterogeneity of glycosylated biotherapeutics","volume":"15","author":"Schachner","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Hu, L., Luo, Y., Yang, J., and Cheng, C. (2025). Botanical Flavonoids: Efficacy, Absorption, Metabolism and Advanced Pharmaceutical Technology for Improving Bioavailability. Molecules, 30.","DOI":"10.3390\/molecules30051184"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Abchir, O., Khedraoui, M., Yamari, I., Nour, H., Errougui, A., Samadi, A., and Chtita, S. (2024). Exploration of alpha-glucosidase inhibitors: A comprehensive in silico approach targeting a large set of triazole derivatives. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0308308"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e202500119","DOI":"10.1002\/cbdv.202500119","article-title":"Pharmacokinetic Prediction and Cytotoxicity of New Quercetin Derivatives","volume":"22","author":"Demonceaux","year":"2025","journal-title":"Chem. Biodivers."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Uczay, M., Santos, P.A., Pfl\u00fcger, P., von Poser, G., Brea, J., Loza, M.I., Pereira, P., and Fontenla, J.A. (2025). In Silico and In Vivo Pharmacological Evaluation of Iridoid Compounds: Geniposide and Asperuloside Profile Study Through Molecular Docking Assay and in the Caenorhabditis elegans Model. Biomolecules, 15.","DOI":"10.3390\/biom15081105"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.chphma.2025.10.007","article-title":"CNS-safe flavone analogues as dual SARS-CoV-2 inhibitors: An integrated in-silico design study","volume":"5","author":"Khibech","year":"2025","journal-title":"ChemPhysMater"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"124830","DOI":"10.1016\/j.carbpol.2025.124830","article-title":"Molecular mechanism and structure-activity relationships of natural source polysaccharides in intervening type 2 diabetes mellitus through antioxidant effects: A systematic review","volume":"377","author":"Yang","year":"2026","journal-title":"Carbohydr. Polym."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"102491","DOI":"10.1016\/j.fochx.2025.102491","article-title":"Plant iridoids: Chemistry, dietary sources and potential health benefits","volume":"27","author":"Guo","year":"2025","journal-title":"Food Chem. X"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"681","DOI":"10.2174\/1389557043403765","article-title":"Predicting Synthetic Accessibility: Application in Drug Discovery and Development","volume":"4","author":"Baber","year":"2012","journal-title":"Mini-Rev. Med. Chem."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Wu, K., Kwon, S.H., Zhou, X., Fuller, C., Wang, X., Vadgama, J., and Wu, Y. (2024). Overcoming Challenges in Small-Molecule Drug Bioavailability: A Review of Key Factors and Approaches. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252313121"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"e71259","DOI":"10.1002\/fsn3.71259","article-title":"Antioxidant Potential of Polyphenolic Compounds, Sources, Extraction, Purification and Characterization Techniques: A Focused Review","volume":"13","author":"Ahmad","year":"2025","journal-title":"Food Sci. Nutr."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"\u0160ovljanski, O., Cvetanovi\u0107 Kljaki\u0107, A., Savelji\u0107, A., and Tomi\u0107, A. (2025). Bioactivity and Bioavailability of Phenols from Plants. Natural Products: Phytochemistry, Botany, Metabolism of Alkaloids, Phenolics and Terpenes, Springer.","DOI":"10.1007\/978-3-642-36202-6_231-1"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Losada-Barreiro, S., Sezgin-Bayindir, Z., Paiva-Martins, F., and Bravo-D\u00edaz, C. (2022). Biochemistry of Antioxidants: Mechanisms and Pharmaceutical Applications. Biomedicines, 10.","DOI":"10.3390\/biomedicines10123051"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1758-2946-1-8","article-title":"Estimation of synthetic accessibility score of drug-like molecules based on molecular complexity and fragment contributions","volume":"1","author":"Ertl","year":"2009","journal-title":"J. Cheminform."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Constantinescu, T., Lungu, C.N., and Lung, I. (2019). Lipophilicity as a central component of drug-like properties of chalchones and flavonoid derivatives. Molecules, 24.","DOI":"10.3390\/molecules24081505"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"43190","DOI":"10.1021\/acsomega.2c05892","article-title":"Investigation of the Influence of Various Functional Groups on the Dynamics of Glucocorticoids","volume":"7","author":"Yadav","year":"2022","journal-title":"ACS Omega"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Unsal, V., Oner, E., Y\u0131ld\u0131z, R., and Mert, B.D. (2025). Comparison of new secondgeneration H1 receptor blockers with some molecules; a study involving DFT, molecular docking, ADMET, biological target and activity. BMC Chem., 19.","DOI":"10.1186\/s13065-024-01371-4"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.2174\/1568026622666220623114450","article-title":"Phenolic Acids\u2014Versatile Natural Moiety with Numerous Biological Applications","volume":"22","author":"Sehrawat","year":"2022","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Charlton, N.C., Mastyugin, M., T\u00f6r\u00f6k, B., and T\u00f6r\u00f6k, M. (2023). Structural Features of Small Molecule Antioxidants and Strategic Modifications to Improve Potential Bioactivity. Molecules, 28.","DOI":"10.3390\/molecules28031057"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Ding, Y., and Xue, X. (2024). Medicinal Chemistry Strategies for the Modification of Bioactive Natural Products. Molecules, 29.","DOI":"10.3390\/molecules29030689"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Yu, Y.Q., Yang, X., Wu, X.F., and Fan, Y. (2021). Bin Enhancing Permeation of Drug Molecules Across the Skin via Delivery in Nanocarriers: Novel Strategies for Effective Transdermal Applications. Front. Bioeng. Biotechnol., 9.","DOI":"10.3389\/fbioe.2021.646554"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"e149156","DOI":"10.3897\/pharmacia.72.e149156","article-title":"Integrating network pharmacology, molecular docking, and molecular dynamics to explore the antidiabetic mechanism of Physalis angulata L","volume":"72","author":"Fitrianingsih","year":"2025","journal-title":"Pharmacia"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Muchtaridi, M., Prasetiawati, R., Alawiah, S.A., Salsabila, S., Fakih, T.M., Nuwarda, R.F., and Ikram, N.K.K. (2025). Revitalizing Recovery: Unveiling the Potential of Apigenin and Related Flavonoids in Long COVID-19 Therapy Through Molecular Dynamics Simulation. Appl. Sci., 15.","DOI":"10.3390\/app15031493"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"355","DOI":"10.18632\/oncotarget.27883","article-title":"Quercetin shortened survival of radio-resistant B-1 cells in vitro and in vivo by restoring miR15a\/16 expression","volume":"12","author":"Ramos","year":"2021","journal-title":"Oncotarget"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"3706","DOI":"10.1038\/s41598-025-33839-1","article-title":"Luteolin alleviates angiogenesis in HUVECs by inhibiting VEGFA expression: Integrating network pharmacology and experimental validation","volume":"16","author":"Yang","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Jang, J.Y., Kim, D., Im, E., Lee, N.K., and Kim, N.D. (2025). Molecular Mechanism Discovery of Acacetin Against Cancers: Insights from Network Pharmacology and Molecular Docking. Int. J. Mol. Sci., 26.","DOI":"10.20944\/preprints202509.0446.v1"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"105574","DOI":"10.1016\/j.phrs.2021.105574","article-title":"Metabolomics and integrated network pharmacology analysis reveal Tricin as the active anti-cancer component of Weijing decoction by suppression of PRKCA and sphingolipid signaling","volume":"171","author":"Li","year":"2021","journal-title":"Pharmacol. Res."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"174555","DOI":"10.1016\/j.ejphar.2021.174555","article-title":"Kaempferide enhances antioxidant capacity to promote osteogenesis through FoxO1\/\u03b2-catenin signaling pathway","volume":"911","author":"Ma","year":"2021","journal-title":"Eur. J. Pharmacol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000527124","article-title":"Mechanism of Glycitein in the Treatment of Colon Cancer Based on Network Pharmacology and Molecular Docking","volume":"16","author":"Xiang","year":"2023","journal-title":"Lifestyle Genom."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"149840","DOI":"10.1016\/j.brainres.2025.149840","article-title":"Daidzein targets PI3K-Akt signaling and oxidative stress in glioblastoma: An Integrated pharmacological and in vitro study","volume":"1863","author":"Praisthy","year":"2025","journal-title":"Brain Res."},{"key":"ref_92","first-page":"1","article-title":"Analysis of the Pharmacodynamic Material Basis and Mechanism of Dendrobium Nobile in the Treatment of Nervous System Diseases Using Integrative Network Pharmacology","volume":"42","author":"Duan","year":"2021","journal-title":"Sci. Technol. Food Ind."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"113284","DOI":"10.1016\/j.intimp.2024.113284","article-title":"Pectolinarigenin alleviates calcium oxalate-induced renal inflammation and oxidative stress by binding to HIF-1\u03b1","volume":"143","author":"Yao","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"497","DOI":"10.4196\/kjpp.24.303","article-title":"Syringetin relieves bone cancer pain in rats induced by breast cancer cells through the ESR1\/PRDM2 axis","volume":"29","author":"Chen","year":"2025","journal-title":"Korean J. Physiol. Pharmacol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"70268","DOI":"10.1002\/mco2.70268","article-title":"Oxidative Stress: Signaling Pathways, Biological Functions, and Disease","volume":"6","author":"Liu","year":"2025","journal-title":"MedComm"},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Zhai, X., Chen, X., Xiao, X., and Wu, L. (2025). Application of network pharmacology in traditional Chinese medicine for the treatment of cardiac diseases. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1703454"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"156887","DOI":"10.1016\/j.phymed.2025.156887","article-title":"Daidzein alleviates ethanol-induced acute gastric injury in rats by targeting ESR1 and activating the PI3K\/AKT\/CREB signaling pathway","volume":"143","author":"Jiang","year":"2025","journal-title":"Phytomedicine"},{"key":"ref_98","first-page":"100942","article-title":"Therapeutic potential, formulation strategies and cancer treatment applications of ripe papaya pectin (Carica papaya L.): A comprehensive review","volume":"11","author":"Wagh","year":"2025","journal-title":"Carbohydr. Polym. Technol. Appl."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"249","DOI":"10.2183\/pjab.101.016","article-title":"Conformational dynamics and molecular interactions of natural products: Unveiling functional structures in biological membranes","volume":"101","author":"Murata","year":"2025","journal-title":"Proc. Jpn. Acad. Ser. B Phys. Biol. Sci."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s12539-015-0263-z","article-title":"Conformational Preferences of \u03c0\u2013\u03c0 Stacking Between Ligand and Protein, Analysis Derived from Crystal Structure Data Geometric Preference of \u03c0\u2013\u03c0 Interaction","volume":"7","author":"Zhao","year":"2015","journal-title":"Interdiscip. Sci.-Comput. Life Sci."},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Sartori, G.R., Albuquerque, A.d.O., Santos-Costa, A.H., Andrade, L.M., da Silva Almeida, D., Gaieta, E.M., Sampaio, J.V., Albuquerque, V.T.d.M.L., and Martins Da Silva, J.H. (2022). In silico mapping of the dynamic interactions and structure-activity relationship of flavonoid compounds against the immune checkpoint programmed-cell death 1 pathway. Front. Drug Discov., 2.","DOI":"10.3389\/fddsv.2022.1032587"},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Aidiel, M., Abdul Mutalib, M., Ramasamy, R., Nik Ramli, N.N., Tang, S.G.H., and Adam, S.H. (2025). Mechanistic Insights into the Anticancer Potential of Methoxyflavones Analogs: A Review. Molecules, 30.","DOI":"10.3390\/molecules30020346"},{"key":"ref_103","first-page":"100233","article-title":"Mechanistic Hydrogen Bond Mediated Screening of Hydroxyl Functionalized Additives for Lignin Condensation Suppression","volume":"2026","author":"Zeng","year":"2026","journal-title":"J. Bioresour. Bioprod."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"102122","DOI":"10.1016\/j.rechem.2025.102122","article-title":"Cytotoxic effects of Methoxy-substituted Chalcones on glioblastoma stem cells: Computational target prediction and therapeutic insights","volume":"14","author":"Veliz","year":"2025","journal-title":"Results Chem."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"e1501240","DOI":"10.1126\/sciadv.1501240","article-title":"Regulation of protein-ligand binding affinity by hydrogen bond pairing","volume":"2","author":"Chen","year":"2016","journal-title":"Sci. Adv."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Sun, Q. (2022). The Hydrophobic Effects: Our Current Understanding. Molecules, 27.","DOI":"10.3390\/molecules27207009"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"100824","DOI":"10.1016\/j.chphi.2025.100824","article-title":"Pharmacophore-based approach for the identification of prospective UDP-2,3-diacylglucosamine hydrolase (LpxH) inhibitor from natural product database against Salmonella Typhi","volume":"10","author":"Karthikeyan","year":"2025","journal-title":"Chem. Phys. Impact"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Lee, J.E., Jayakody, J.T.M., Kim, J.I., Jeong, J.W., Choi, K.M., Kim, T.S., Seo, C., Azimi, I., Hyun, J.M., and Ryu, B.M. (2024). The Influence of Solvent Choice on the Extraction of Bioactive Compounds from Asteraceae: A Comparative Review. Foods, 13.","DOI":"10.3390\/foods13193151"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ymeth.2021.10.007","article-title":"Comparative analysis of network-based approaches and machine learning algorithms for predicting drug-target interactions","volume":"198","author":"Jung","year":"2022","journal-title":"Methods"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"100125","DOI":"10.1016\/j.crstbi.2024.100125","article-title":"Targeting EGFR allosteric site with marine-natural products of Clathria Sp.: A computational approach","volume":"7","author":"Nurisyah","year":"2024","journal-title":"Curr. Res. Struct. Biol."},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"Ziemba, B. (2025). Advances in Cytotoxicity Testing: From In Vitro Assays to In Silico Models. Int. J. Mol. Sci., 26.","DOI":"10.20944\/preprints202510.1961.v1"},{"key":"ref_112","unstructured":"Nanotechnology Characterization Laboratory (2023). Guide to NCL In Vivo Studies: Efficacy, Pharmacokinetics & Toxicology."},{"key":"ref_113","doi-asserted-by":"crossref","unstructured":"Priani, S.E., Fakih, T.M., Wilar, G., Chaerunisaa, A.Y., and Sopyan, I. (2025). Quality by Design and In Silico Approach in SNEDDS Development: A Comprehensive Formulation Framework. Pharmaceutics, 17.","DOI":"10.3390\/pharmaceutics17060701"},{"key":"ref_114","doi-asserted-by":"crossref","unstructured":"Kobayashi, H., Shigetomi, H., Nishio, M., Umetani, M., Imanaka, S., and Hashimoto, H. (2025). Molecular Regulation of FOXO1 and Its Pathophysiological Significance in Endometriosis: A Narrative Review. Antioxidants, 15.","DOI":"10.3390\/antiox15010003"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"100113","DOI":"10.1016\/j.arres.2024.100113","article-title":"Phytochemical-mediated modulation of signaling pathways: A promising avenue for drug discovery","volume":"13","author":"Paul","year":"2024","journal-title":"Adv. Redox Res."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.jpha.2023.06.011","article-title":"Integrative multi-omics and systems bioinformatics in translational neuroscience: A data mining perspective","volume":"13","author":"Lim","year":"2023","journal-title":"J. Pharm. Anal."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"10782","DOI":"10.1021\/acsomega.0c00379","article-title":"Molecular Mechanisms Underlying the Absorption of Aglycone and Glycosidic Flavonoids in a Caco-2 BBe1 Cell Model","volume":"5","author":"Zhang","year":"2020","journal-title":"ACS Omega"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"100948","DOI":"10.1016\/j.focha.2025.100948","article-title":"Leveraging quantitative structure-activity relationships to design and optimize wall material formulations for antioxidant encapsulation via spray drying","volume":"7","author":"Lisboa","year":"2025","journal-title":"Food Chem. Adv."},{"key":"ref_119","doi-asserted-by":"crossref","unstructured":"Serrano, D.R., Luciano, F.C., Anaya, B.J., Ongoren, B., Kara, A., Molina, G., Ramirez, B.I., S\u00e1nchez-Guirales, S.A., Simon, J.A., and Tomietto, G. (2024). Artificial Intelligence (AI) Applications in Drug Discovery and Drug Delivery: Revolutionizing Personalized Medicine. 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