{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T08:40:31Z","timestamp":1785400831401,"version":"3.56.0"},"reference-count":79,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004412","name":"T\u00dcBA","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004412","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/00aqt9352","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Brassicaceae plants, among the most widely consumed vegetables worldwide, are recognized as rich sources of biologically active compounds. In this study, nine species belonging to the cruciferous genus Thlaspi were investigated, including T. alliaceum, T. arvense, T. violascens, T. aghricum, T. cataonicum, T. annuum, T. watsonii, T. cariense, and T. elegans. In the past, pennycress species were consumed to alleviate hunger and provide nutritional support during periods of food scarcity. To evaluate the antioxidant capacities of methanol and water extracts obtained from Thlaspi species, several complementary assays were employed, including 2,2\u2032-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical (ABTS\u2022+) scavenging, 1,1-diphenyl-2-picrylhydrazyl free radicals (DPPH\u2022) scavenging, N,N-dimethylphenylenediamine radicals (DMPD\u2022+) scavenging, Fe3+-2,4,6-tris(2-pyridyl)-s-triazine (TPTZ)-reducing, Fe3+ ion-reducing, and Cu2+ ion-reducing assays. The IC50 values of both methanol and water extracts from the aerial parts of Thlaspi species for ABTS\u2022+, DPPH\u2022, and DMPD\u2022+ scavenging activities were studied compared with antioxidant standards, including \u03b1-tocopherol, Trolox, butylated hydroxytoluene (BHA), and butylated hydroxyanisole (BHT). The total phenolic and flavonoid contents of the extracts ranged from 8.29 to 49.14 mg gallic acid equivalent (GAE)\/g extract and from 2.33 to 74.66 mg quercetin equivalent (QE)\/g extract, respectively. Furthermore, the inhibitory effects of water and methanol extracts of Thlaspi species against \u03b1-amylase, acetylcholinesterase (AChE), and carbonic anhydrase (CA II) enzymes were evaluated. The IC50 values were determined to range from 122.4 to 245.9 \u03bcg\/mL for \u03b1-amylase, from 17.3 to 24.1 \u03bcg\/mL for AChE, and from 41.9 to 256.5 \u03bcg\/mL for CA II inhibition. In addition, the phenolic profiles of Thlaspi species were comprehensively characterized by LC-MS\/MS analysis using 53 reference standards. The findings demonstrated that the aerial parts of Thlaspi species are rich in polyphenolic antioxidants and may serve as promising natural sources with potential applications in the management of diabetes, Alzheimer\u2019s disease (AD), glaucoma, epilepsy, and cancer.<\/jats:p>","DOI":"10.3390\/plants15142207","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T06:09:09Z","timestamp":1784527749000},"page":"2207","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Assessment of Bioactive Properties of Nine Thlaspi Species: Antioxidant Activity, Enzyme Inhibition and LC\u2013MS\/MS Polyphenolic Characterization"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6912-3998","authenticated-orcid":false,"given":"Hasan","family":"Karage\u00e7ili","sequence":"first","affiliation":[{"name":"Department of Nursing, Faculty of Health Sciences, Siirt University, 56100 Siirt, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeynebe","family":"Bing\u00f6l","sequence":"additional","affiliation":[{"name":"Department of Medical Services and Techniques, Tokat Vocational School of Health Services, Gaziosmanpasa University, 60250 Tokat, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4090-7227","authenticated-orcid":false,"given":"Mustafa Abdullah","family":"Y\u0131lmaz","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Department of Analytical Chemistry, Dicle University, 21280 Diyarbak\u0131r, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet","family":"Fidan","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science and Arts, Siirt University, 56100 Siirt, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7363-1478","authenticated-orcid":false,"given":"Mehmet Cengiz","family":"Karaismailo\u011flu","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Sciences, Bart\u0131n University, 74100 Siirt, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5453-0953","authenticated-orcid":false,"given":"Hulya","family":"Ak\u0131nc\u0131oglu","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Department of Biochemistry, Agri Ibrahim Cecen University, 04100 Agri, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5993-1668","authenticated-orcid":false,"given":"\u0130lhami","family":"Gulcin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Ataturk University, 25240 Erzurum, T\u00fcrkiye"},{"name":"Rectorate of Agri Ibrahim Cecen University, 04100 Agri, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"101441","DOI":"10.1016\/j.bcab.2019.101441","article-title":"Anticholinergic, antidiabetic and antioxidant activities of Anatolian pennyroyal (Mentha pulegium): Analysis of its polyphenol contents by LC-MS\/MS","volume":"23","author":"Gulcin","year":"2020","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.bcab.2018.09.020","article-title":"Phytochemical profile and antioxidant properties of two Brassicaceae species: Cardaria draba subsp. draba and Descurainia sophia","volume":"16","author":"Mahomoodally","year":"2018","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"139","DOI":"10.23902\/trkjnat.762509","article-title":"Notes on leaf and stem anatomy of Thlaspi sensu lato","volume":"21","author":"Erol","year":"2020","journal-title":"Trak. Univ. J. Nat. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"197","DOI":"10.37427\/botcro-2022-017","article-title":"A systematic study of the Thlaspi s. L. taxa in sections Nomisma, Thlaspi and Pterotropis from Turkey based on fruit morphological and molecular data","volume":"81","author":"Inal","year":"2022","journal-title":"Acta Bot. Croat."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1007\/s11627-023-10378-w","article-title":"An improved micropropagation protocol for the ex situ conservation of Thlaspi cariense A. Carlstr\u00f6m (Brassicaceae): An endangered Turkish endemic plant species","volume":"59","year":"2023","journal-title":"In Vitro Cell. Dev. Biol. Plant"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e16411","DOI":"10.1111\/jfpp.16411","article-title":"Purification, characterization and antioxidant activity of polysaccharides from okara","volume":"46","author":"Hu","year":"2022","journal-title":"J. Food Process. Preserv."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"116814","DOI":"10.1016\/j.jep.2023.116814","article-title":"Preliminary study on the material basis and mechanism underlying uric acid reduction by Thlaspi arvense L","volume":"319","author":"Ke","year":"2024","journal-title":"J. Ethnopharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Koksal, E., Tohma, S.H., K\u0131l\u0131\u00e7, \u00d6., Alan, Y., Aras, A., Gulcin, I., and Bursal, E. (2017). Assessment of antimicrobial and antioxidant activities of Nepeta trachonitica: Analysis of its phenolic compounds using HPLC-MS\/MS. Sci. Pharm., 85.","DOI":"10.3390\/scipharm85020024"},{"key":"ref_9","first-page":"388","article-title":"Antioxidants: Current summary","volume":"42","author":"Gulcin","year":"2025","journal-title":"Balk. Med. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1002\/ardp.201100272","article-title":"Synthesis and antioxidant properties of (3,4-dihydroxyphenyl)(2,3,4-trihydroxyphenyl)methanone and its derivatives","volume":"345","author":"Menzek","year":"2012","journal-title":"Arch. Pharm."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1556\/AAlim.34.2005.2.13","article-title":"Evaluation of antioxidant activity of cornelian cherry (Cornus mas L.)","volume":"34","author":"Gulcin","year":"2005","journal-title":"Acta Aliment."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Xu, L., and Wang, X. (2025). A comprehensive review of phenolic compounds in horticultural plants. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26125767"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e71259","DOI":"10.1002\/fsn3.71259","article-title":"Antioxidant potential of polyphenolic compounds, sources, extraction, purification and characterization techniques: A focused review","volume":"13","author":"Ahmad","year":"2025","journal-title":"Food Sci. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.tifs.2017.11.008","article-title":"Antioxidants: Reviewing the chemistry, food applications, legislation and role as preservatives","volume":"71","author":"Carocho","year":"2018","journal-title":"Trends Food Sci. Technol."},{"key":"ref_15","first-page":"496","article-title":"Antioxidant activity and polyphenol content of cranberries (Vaccinium macrocarpon)","volume":"9","author":"Gulcin","year":"2015","journal-title":"Rec. Nat. Prod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"384S","DOI":"10.3945\/an.112.003517","article-title":"Health-promoting components of fruits and vegetables in the diet","volume":"4","author":"Liu","year":"2013","journal-title":"Adv. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"101711","DOI":"10.1016\/j.bcab.2020.101711","article-title":"Assessments of anticholinergic, antidiabetic, antioxidant activities and phenolic content of Stachys annua","volume":"28","author":"Bursal","year":"2020","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_18","first-page":"17","article-title":"Evaluation of the in vitro antioxidant properties of broccoli extracts (Brassica oleracea L.)","volume":"16","author":"Gulcin","year":"2004","journal-title":"Ital. J. Food. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Karagecili, H., \u0130zol, E., Kire\u00e7ci, E., and Gulcin, \u0130. (2023). Determination of antioxidant, anti-Alzheimer, antidiabetic, antiglaucoma and antimicrobial effects of Zivzik pomegranate (Punica granatum): A chemical profiling by LC-MS\/MS. Life, 13.","DOI":"10.3390\/life13030735"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/j.jff.2015.06.018","article-title":"Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects\u2014A review","volume":"18","author":"Shahidi","year":"2015","journal-title":"J. Funct. Foods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1002\/ardp.201300273","article-title":"Novel sulfamides and sulfonamides incorporating tetralin scaffold as carbonic anhydrase and acetylcholinesterase inhibitors","volume":"347","author":"Topal","year":"2014","journal-title":"Arch. Pharm."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"102931","DOI":"10.1016\/j.bioorg.2019.102931","article-title":"Novel eugenol-bearing oxypropanolamines: Synthesis, characterization, antibacterial, antidiabetic and anticholinergic potentials","volume":"88","author":"Kestane","year":"2019","journal-title":"Bioorg. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Grabowska, W., Bijak, M., Szelenberger, R., Gorniak, L., Podogrocki, M., Harmata, P., and Cichon, N. (2025). Acetylcholinesterase as a multifunctional target in amyloid-driven neurodegeneration: From dual-site inhibitors to anti-aggregation strategies. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26178726"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1038\/s41583-019-0132-6","article-title":"Oxidative stress, dysfunctional glucose metabolism and Alzheimer disease","volume":"20","author":"Butterfield","year":"2019","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e22385","DOI":"10.1002\/jbt.22385","article-title":"Inhibition profiles of voriconazole against acetylcholinesterase, \u03b1-glycosidase and human carbonic anhydrase I and II isoenzymes","volume":"33","author":"Topal","year":"2019","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/j.ijbiomac.2016.10.096","article-title":"Novel eugenol derivatives: Potent acetylcholinesterase and carbonic anhydrase inhibitors","volume":"94","author":"Topal","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1016\/j.biopha.2017.09.013","article-title":"Ameliorative impact of Morus alba leaves aqueous extract against embryonic ophthalmic tissue malformation in streptozotocin-induced diabetic rats","volume":"95","author":"Assiri","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1002\/bab.1781","article-title":"Anti-diabetic properties of dietary phenolic compounds: Inhibition effects on \u03b1-amylase, aldose reductase and \u03b1-glycosidase","volume":"66","author":"Demir","year":"2019","journal-title":"Biotechnol. Appl. Biochem."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Supuran, C.T. (2022). Carbonic anhydrase inhibitors from marine natural products. Mar. Drugs, 20.","DOI":"10.3390\/md20110721"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.bioorg.2014.07.009","article-title":"Carbonic anhydrase inhibitory properties of novel benzylsulfamides using molecular modeling and experimental studies","volume":"56","author":"Naderi","year":"2014","journal-title":"Bioorg. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1021\/acs.chemrev.4c00278","article-title":"Multiple binding modes of inhibitors to human carbonic anhydrases: An update on the design of isoform-specific modulators of activity","volume":"125","author":"Alterio","year":"2025","journal-title":"Chem. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3109\/14756366.2012.750311","article-title":"Carbonic anhydrase inhibitory properties of novel sulfonamide derivatives of aminoindanes and aminotetralins","volume":"29","author":"Akbaba","year":"2014","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e70088","DOI":"10.1002\/prp2.70088","article-title":"Sulfonamide-bearing pyrazolone derivatives as multitarget therapeutic agents: Design, synthesis, characterization, biological evaluation, in silico ADME\/T profiling and molecular docking study","volume":"13","author":"Lolak","year":"2025","journal-title":"Pharmacol. Res. Perspect."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Llorca, A., Carta, F., Supuran, C.T., and Eysteinsson, T. (2024). Carbonic anhydrase, its inhibitors and vascular function. Front. Mol. Biosci., 11.","DOI":"10.3389\/fmolb.2024.1338528"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1517\/17425255.2016.1154534","article-title":"Drug interaction considerations in the therapeutic use of carbonic anhydrase inhibitors","volume":"12","author":"Supuran","year":"2016","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"239","DOI":"10.25135\/rnp.24.17.08.138","article-title":"Antioxidant capacity connection with phenolic and flavonoid content in Chinese medicinal herbs","volume":"12","author":"Li","year":"2018","journal-title":"Rec. Nat. Prod."},{"key":"ref_37","first-page":"18","article-title":"Antioxidant activity and the polyphenolic and flavonoid contents of five high altitude medicinal plants used in Bhutanese Sowa rigpa Medicine","volume":"7","author":"Yeshi","year":"2017","journal-title":"J. Biol. Act. Prod. Nat."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.sajb.2024.05.031","article-title":"Evaluation of antioxidant activities and inhibition effects of Tribulus terrestris L. extracts on some metabolic enzymes","volume":"170","author":"Kaya","year":"2024","journal-title":"S. Afr. J. Bot."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1017\/S1461145705005833","article-title":"Targeting acetylcholinesterase and butyrylcholinesterase in dementia","volume":"9","author":"Lane","year":"2006","journal-title":"Int. J. Neuropsychopharmacol."},{"key":"ref_40","first-page":"92","article-title":"Measurement of anticancer, antidiabetic and anticholinergic properties of sumac (Rhus coriaria): Analysis of its phenolic compounds by LC-MS\/MS","volume":"112\u2013113","author":"Tohma","year":"2012","journal-title":"Aquat. Toxicol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.sajb.2022.11.012","article-title":"The determination of antidiabetic, anticholinesterase and antioxidant properties of ethanol and water extracts of blackberry (Rubus fruticosus L.) fruits at different maturity stages","volume":"151","year":"2022","journal-title":"S. Afr. J. Bot."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"586","DOI":"10.3109\/14756366.2014.956310","article-title":"Carbonic anhydrase inhibitors: Guaiacol and catechol derivatives effectively inhibit certain human carbonic anhydrase isoenzymes (hCA I, II, IX and XII)","volume":"30","author":"Scozzafava","year":"2015","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"402","DOI":"10.3109\/14756366.2012.670807","article-title":"(3,4-Dihydroxyphenyl)(2,3,4-trihydroxyphenyl)methanone and its derivatives as carbonic anhydrase isoenzymes inhibitors","volume":"28","author":"Nar","year":"2013","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1080\/14756360310001656736","article-title":"Effect of melatonin on carbonic anhydrase from human erythrocyte in vitro and from rat erythrocyte in vivo","volume":"19","author":"Beydemir","year":"2004","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Usjak, L., Skalicka-Wozniak, K., Kulinowski, L., Swi\u0105tek, L., Milutinovic, V., Salwa, K., Boguszewska, A., Korona-Glowniak, I., Susniak, K., and Niketis, M. (2026). Integrated phytochemical profiling and bioactivity evaluation of Micromeria nervosa, with emphasis on antimicrobial and antiviral properties. Antibiotics, 15.","DOI":"10.3390\/antibiotics15040374"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Karagecili, H., Y\u0131lmaz, M.A., Ert\u00fcrk, A., K\u0131z\u0131lta\u015f, H., G\u00fcven, L., Alwasel, S.H., and Gulcin, \u0130. (2023). Comprehensive metabolite profiling of Berdav propolis using LC-MS\/MS: Determination of antioxidant, anticholinergic, antiglaucoma and antidiabetic effects. Molecules, 28.","DOI":"10.3390\/molecules28041739"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s43014-025-00326-z","article-title":"How to assess antioxidant activity? Advances, limitations, and applications of in vitro, in vivo, and ex vivo approaches","volume":"7","author":"Shahidi","year":"2025","journal-title":"Food Prod. Process. Nutr."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s43094-020-00161-8","article-title":"Flavonoids as natural phenolic compounds and their role in therapeutics: An overview","volume":"7","author":"Mutha","year":"2021","journal-title":"Fut. J. Pharm. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Gulcin, \u0130., and Alwasel, S.H. (2022). Metal ions, metal chelators and metal chelating assay as antioxidant method. Processes, 10.","DOI":"10.3390\/pr10010132"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"7313","DOI":"10.3390\/molecules15107313","article-title":"Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties","volume":"15","author":"Dai","year":"2010","journal-title":"Molecules"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"307","DOI":"10.5264\/eiyogakuzashi.44.307","article-title":"Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine","volume":"44","author":"Oyaizu","year":"1986","journal-title":"Jpn. J. Nutr. Diet."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Durmaz, L., Karage\u00e7ili, H., Ert\u00fcrk, A., Ozden, E.M., Taslimi, P., Alwasel, S., and G\u00fcl\u00e7in, I. (2024). Hamamelitannin\u2019s antioxidant effect and its inhibition capability on \u03b1-glycosidase, carbonic anhydrase, acetylcholinesterase and butyrylcholinesterase enzymes. Processes, 12.","DOI":"10.3390\/pr12112341"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Durmaz, L., Karagecili, H., and Gulcin, I. (2023). Evaluation of carbonic anhydrase, acetylcholinesterase, butyrylcholinesterase and \u03b1-glycosidase inhibition effects and antioxidant activity of baicalin hydrate. Life, 13.","DOI":"10.3390\/life13112136"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"117139","DOI":"10.1016\/j.foodres.2025.117139","article-title":"Comparative analysis of chemical composition and biological activities of Punica granatum L. extracts: UPLC-MS\/MS profiling, antioxidant capacity, enzyme inhibition and molecular docking","volume":"220","author":"Duysak","year":"2025","journal-title":"Food Res. Int."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"4290","DOI":"10.1021\/jf0502698","article-title":"Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements","volume":"53","author":"Prior","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1016\/j.foodchem.2009.11.065","article-title":"Comparison of total antioxidant capacity and phenolic composition of some apple juices with combined HPLC-CUPRAC assay","volume":"120","author":"Karaman","year":"2010","journal-title":"Food Chem."},{"key":"ref_57","first-page":"65","article-title":"Antioxidant activity of cauliflower (Brassica oleracea L.)","volume":"32","author":"Gulcin","year":"2008","journal-title":"Turk. J. Agric. For."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"144","DOI":"10.5344\/ajev.1965.16.3.144","article-title":"Colorimetry of total phenolics with phosphomolybdic\u2013phosphotungstic acid reagents","volume":"16","author":"Singleton","year":"1965","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Tahiro\u011flu, V., Karagecili, H., Aslan, K., and Gulcin, I. (2025). Polyphenolic profiling and evaluation of antioxidant, antidiabetic, anti-Alzheimer and antiglaucoma activities of Allium kharputense and Anchusa azurea var. azurea. Life, 15.","DOI":"10.3390\/life15081209"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2481148","DOI":"10.1080\/10942912.2025.2481148","article-title":"Phytochemical profile and bioactive properties of sage (Salvia fruticosa) and thyme (Thymus vulgaris) extracts","volume":"28","author":"Aslan","year":"2025","journal-title":"Int. J. Food Prop."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1111\/j.1750-3841.2011.02142.x","article-title":"Pomological features, nutritional quality, polyphenol content analysis and antioxidant properties of domesticated and wild ecotypes of raspberry (Rubus idaeus L.)","volume":"76","author":"Gulcin","year":"2011","journal-title":"J. Food Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"112347","DOI":"10.1016\/j.indcrop.2020.112347","article-title":"Simultaneous quantitative screening of 53 phytochemicals in medicinal and aromatic plants by LC\u2013MS\/MS","volume":"149","year":"2020","journal-title":"Ind. Crops Prod."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1002\/pca.3114","article-title":"A new method for isolation of tomentosin from Inula viscosa (L.) and determination of its bioactivities","volume":"33","year":"2022","journal-title":"Phytochem. Anal."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"7970","DOI":"10.1021\/jf048741x","article-title":"Novel total antioxidant capacity index using cupric ion reducing capability: CUPRAC method","volume":"52","author":"Apak","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"20230120","DOI":"10.1515\/chem-2023-0120","article-title":"Antioxidant, antidiabetic, antiglaucoma and anticholinergic effects of Tayfi grape (Vitis vinifera): Phytochemical screening by LC-MS\/MS","volume":"21","author":"Gulcin","year":"2023","journal-title":"Open Chem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1002\/1099-1573(200008)14:5<323::AID-PTR621>3.0.CO;2-Q","article-title":"Antioxidant activity of Nigella sativa essential oil","volume":"14","author":"Burits","year":"2000","journal-title":"Phytother. Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1038\/1811199a0","article-title":"Antioxidant determinations by the use of a stable free radical","volume":"181","author":"Blois","year":"1958","journal-title":"Nature"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Gulcin, \u0130., and Alwasel, S.H. (2023). DPPH radical scavenging assay. Processes, 11.","DOI":"10.3390\/pr11082248"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Bibi Sadeer, N., Montesano, D., Albrizio, S., Zengin, G., and Mahomoodally, M.F. (2020). The versatility of antioxidant assays in food science and safety. Antioxidants, 9.","DOI":"10.3390\/antiox9080709"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1021\/jf980496s","article-title":"Method for measuring antioxidant activity and its application to wines","volume":"47","author":"Fogliano","year":"1999","journal-title":"J. Agric. Food Chem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1080\/14756360701626223","article-title":"Measurement of antioxidant ability of melatonin and serotonin by DMPD and CUPRAC methods","volume":"23","author":"Gulcin","year":"2008","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A new and rapid colorimetric determination of acetylcholinesterase activity","volume":"7","author":"Ellman","year":"1961","journal-title":"Biochem. Pharmacol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.indcrop.2018.01.031","article-title":"Activity-guided fractionation of pomegranate extract and its bioactivities","volume":"113","author":"Savikin","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.ab.2006.01.036","article-title":"A quantitative starch\u2013iodine method for measuring \u03b1-amylase and glucoamylase activities","volume":"351","author":"Xiao","year":"2006","journal-title":"Anal. Biochem."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"\u00d6zden, E.M., Bing\u00f6l, Z., Mutlu, M., Karagecili, H., K\u00f6ksal, E., G\u00f6ren, A.C., Alwasel, S.H., and Gulcin, \u0130. (2023). Antioxidant, antiglaucoma, anticholinergic and antidiabetic effects of kiwifruit (Actinidia deliciosa) oil: LC-HR\/MS, GC\/MS and GC-FID analysis. Life, 13.","DOI":"10.3390\/life13091939"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein","volume":"67","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"4221","DOI":"10.1016\/S0021-9258(18)95800-X","article-title":"Esterase activities of human carbonic anhydrases B and C","volume":"242","author":"Verpoorte","year":"1967","journal-title":"J. Biol. Chem."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.3109\/14756366.2016.1156104","article-title":"Synthesis of some tetrahydropyrimidine-5-carboxylates, determination of their metal chelating effects and inhibition profiles against acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase","volume":"31","author":"Sujayev","year":"2016","journal-title":"J. Enzyme Inhib. Med. Chem."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/14\/2207\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T04:10:37Z","timestamp":1784693437000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/14\/2207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,20]]},"references-count":79,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["plants15142207"],"URL":"https:\/\/doi.org\/10.3390\/plants15142207","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,20]]}}}