{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:29:16Z","timestamp":1785403756099,"version":"3.56.0"},"reference-count":36,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"scholarship from PRINT","doi-asserted-by":"publisher","award":["88887.877807\/2023-00"],"award-info":[{"award-number":["88887.877807\/2023-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"award":["88887.877807\/2023-00"],"award-info":[{"award-number":["88887.877807\/2023-00"]}],"id":[{"id":"https:\/\/ror.org\/00x0ma614","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["444090\/2024-0"],"award-info":[{"award-number":["444090\/2024-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["305520\/2022-9"],"award-info":[{"award-number":["305520\/2022-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"award":["444090\/2024-0"],"award-info":[{"award-number":["444090\/2024-0"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]},{"award":["305520\/2022-9"],"award-info":[{"award-number":["305520\/2022-9"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland Frontiers for the Future Programme","doi-asserted-by":"publisher","award":["19\/FFP\/6499"],"award-info":[{"award-number":["19\/FFP\/6499"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Talisia esculenta Radlk (Sapindaceae) is a tropical tree species native to Brazil, whose leaves are traditionally consumed as herbal infusions. However, the secondary metabolite composition of its foliage remains poorly characterised. In this study, the phytochemical profile of T. esculenta leaf infusions was investigated using HPLC\u2013ESI\u2013QTOF\u2013MS\/MS, leading to the annotation of 20 compounds. Subsequent liquid\u2013liquid partitioning and high-speed counter-current chromatography (HSCCC) enabled the targeted isolation of two major constituents. The analysis revealed a flavonoid-rich profile dominated by glycosylated flavonols, with rutin and quercitrin identified as the principal compounds and structurally confirmed by NMR spectroscopy. They were both quantified in the aqueous infusion of the leaves, being 1.42 \u00b1 0.02% (w\/w) and 1.54 \u00b1 0.01% (w\/w) for rutin and quercitrin, respectively. The metabolite pattern found is consistent with that reported in other members of the Sapindaceae family and contributes to the chemotaxonomic characterisation of the genus. Fractionation enhanced the enrichment of phenolic constituents and allowed assessment of their functional properties such as antioxidant activity (including DPPH assay: from 43.4% for the aqueous infusion to 92.9% for the ethyl acetate fraction) and inhibition of microbial biofilm formation (from 37.4% for the aqueous infusion to 83.4% for the butanolic fraction at 6.25 \u00b5g\/mL against Staphylococcus aureus). The MIC and MBC were also calculated for the aqueous infusion (12.5 mg\/mL for both), its partitions as well as rutin and quercitrin. Anti-inflammatory effects were also observed, with inhibition of NF-\u03baB activity reaching 56.9% for the aqueous infusion and 47.7% for the butanolic fraction. These findings expand current knowledge of secondary metabolite diversity in T. esculenta and demonstrate the applicability of HSCCC as an efficient technique for flavonoid isolation from complex plant extracts. Overall, this study provides new insights into the phytochemical composition of an underexplored tropical species within the Sapindaceae family.<\/jats:p>","DOI":"10.3390\/plants15142229","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:17:52Z","timestamp":1784647072000},"page":"2229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Characterization and High-Speed Countercurrent Chromatography-Assisted Isolation of Flavonoids from Leaves of Talisia esculenta Radlk"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4690-2785","authenticated-orcid":false,"given":"Katia Castanho","family":"Scortecci","sequence":"first","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, D02 R590 Dublin, Ireland"},{"name":"Laborat\u00f3rio de Transforma\u00e7\u00e3o de Plantas e An\u00e1lise em Microscopia (LTPAM), Departamento de Biologia Celular e Gen\u00e9tica, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioqu\u00edmica e Biologia Molecular, Centro de Bioci\u00eancias, Universidade Federal do Rio Grande do Norte, Natal 59078-970, Brazil"},{"name":"School of Microbiology and APC Microbiome Ireland, University College Cork, T12 YT20 Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6976-1938","authenticated-orcid":false,"given":"Let\u00edcia Barbosa","family":"Santos","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, D02 R590 Dublin, Ireland"},{"name":"Pharmaceutical Products Department, College of Pharmacy, Federal University of Minas Gerais, Belo Horizonte 31270-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8987-4769","authenticated-orcid":false,"given":"Lu\u00eds Felipe Costa","family":"Gatis","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Transforma\u00e7\u00e3o de Plantas e An\u00e1lise em Microscopia (LTPAM), Departamento de Biologia Celular e Gen\u00e9tica, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioqu\u00edmica e Biologia Molecular, Centro de Bioci\u00eancias, Universidade Federal do Rio Grande do Norte, Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f4nica Giuliani de Queiroz","family":"Aquino-Martins","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Transforma\u00e7\u00e3o de Plantas e An\u00e1lise em Microscopia (LTPAM), Departamento de Biologia Celular e Gen\u00e9tica, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Bioqu\u00edmica e Biologia Molecular, Centro de Bioci\u00eancias, Universidade Federal do Rio Grande do Norte, Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1227-4116","authenticated-orcid":false,"given":"Nathalia Maira Cabral","family":"de Medeiros","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Transforma\u00e7\u00e3o de Plantas e An\u00e1lise em Microscopia (LTPAM), Departamento de Biologia Celular e Gen\u00e9tica, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7076-600X","authenticated-orcid":false,"given":"Wirginia","family":"Kukula-Koch","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Lublin, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9622-7629","authenticated-orcid":false,"given":"Sarah","family":"Stiegeler","sequence":"additional","affiliation":[{"name":"School of Microbiology and APC Microbiome Ireland, University College Cork, T12 YT20 Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garance","family":"Coquant","sequence":"additional","affiliation":[{"name":"School of Microbiology and APC Microbiome Ireland, University College Cork, T12 YT20 Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5531-1787","authenticated-orcid":false,"given":"Fabio","family":"Boylan","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, D02 R590 Dublin, Ireland"},{"name":"Trinity Natural Products Research Centre, 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