{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:29:17Z","timestamp":1785414557354,"version":"3.56.0"},"reference-count":43,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T00:00:00Z","timestamp":1785024000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Major Science and Technology Special Project of Xinjiang Uygur Autonomous Region","award":["2024A02007-2"],"award-info":[{"award-number":["2024A02007-2"]}]},{"name":"earmarked fund for XJARS","award":["XJARS-06"],"award-info":[{"award-number":["XJARS-06"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32060688"],"award-info":[{"award-number":["32060688"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32060688"],"award-info":[{"award-number":["32060688"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Verticillium dahliae is a devastating soil-borne fungal pathogen that causes severe yield losses in melon (Cucumis melo L.) and other crops. Identifying novel resistance genes is crucial for sustainable disease management. In this study, we characterized the function of CmVQ23, a candidate gene previously identified through QTL mapping, in mediating defense against V. dahliae using heterologous expression in Arabidopsis thaliana. Subcellular localization assays revealed that the CmVQ23-eGFP fusion protein predominantly localized to the nucleus, consistent with its predicted role as a co-factor of transcription factor. Upon V. dahliae inoculation, CmVQ23-overexpressing Arabidopsis lines exhibited significantly reduced disease indices and restricted fungal proliferation compared with wild-type and mutant plants, although these lines displayed altered vegetative growth, including delayed bolting and reduced plant height. Mechanistically, CmVQ23 overexpression promoted reactive oxygen species (ROS) accumulation and hypersensitive response (HR)-mediated cell death at infection sites, as evidenced by intensified DAB and trypan blue staining. Furthermore, transgenic lines maintained higher photosynthetic efficiency, enhanced antioxidant enzyme activities, and increased lignin deposition via upregulation of phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO). Notably, CmVQ23 overexpression markedly upregulated both salicylic acid (SA)- and jasmonic acid\/ethylene (JA\/ET)-responsive marker genes, including AtPR1, AtPR2, AtPR5, AtPAD4, AtPDF1.2, and AtVSP2 upon infection. Collectively, these findings demonstrate that CmVQ23 functions as a positive regulator of resistance to Verticillium dahliae by orchestrating ROS\/HR-mediated cell death, antioxidant defense, phenylpropanoid pathway activation, and phytohormone signaling crosstalk, offering a promising genetic resource for improving Verticillium wilt resistance in crops.<\/jats:p>","DOI":"10.3390\/plants15152283","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T01:26:23Z","timestamp":1785115583000},"page":"2283","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Heterologous Expression of the Melon CmVQ23 Positively Regulates Resistance to Verticillium dahliae in Arabidopsis"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6130-0811","authenticated-orcid":false,"given":"Peifeng","family":"Yu","sequence":"first","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetics Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simin","family":"Lu","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetics Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8382-9742","authenticated-orcid":false,"given":"Jiyang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetics Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianlei","family":"Wang","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetics Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefei","family":"Ning","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetics Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.plaphy.2018.02.011","article-title":"Physiological and molecular mechanism of defense in cotton against Verticillium dahliae","volume":"125","author":"Shaban","year":"2018","journal-title":"Plant Physiol. Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Meshram, S., and Adhikari, T.B. (2024). Microbiome-Mediated Strategies to Manage Major Soil-Borne Diseases of Tomato. Plants, 13.","DOI":"10.3390\/plants13030364"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Gim\u00e9nez-Santamarina, S., Torres-Pagan, N., Larran, S., Rosell\u00f3, J., and Santamarina, M.P. (2025). Clove Essential Oil and Eugenol as Natural Antifungal Agents to Reduce Postharvest Losses in Melon (Cucumis melo). Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26199603"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"8388","DOI":"10.1111\/pce.70124","article-title":"WRKY Transcription Factors in Rice: Key Regulators Orchestrating Development and Stress Resilience","volume":"48","author":"Li","year":"2025","journal-title":"Plant Cell Environ."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1111\/tpj.13619","article-title":"Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function","volume":"91","author":"Lei","year":"2017","journal-title":"Plant J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3824","DOI":"10.1105\/tpc.111.090571","article-title":"Arabidopsis sigma factor binding proteins are activators of the WRKY33 transcription factor in plant defense","volume":"23","author":"Lai","year":"2011","journal-title":"Plant Cell"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1111\/nph.70072","article-title":"A molecular switch OsWRKY10-OsVQ8 orchestrates rice diterpenoid phytoalexin biosynthesis for broad-spectrum disease resistance","volume":"246","author":"Lin","year":"2025","journal-title":"New Phytol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1093\/plphys\/kiac275","article-title":"SlVQ15 interacts with jasmonate-ZIM domain proteins and SlWRKY31 to regulate defense response in tomato","volume":"190","author":"Huang","year":"2022","journal-title":"Plant Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/pbi.14493","article-title":"SlVQ15 recruits SlWRKY30IIc to link with jasmonate pathway in regulating tomato defence against root-knot nematodes","volume":"23","author":"Huang","year":"2025","journal-title":"Plant Biotechnol. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1111\/nph.12817","article-title":"The Arabidopsis thaliana mitogen-activated protein kinases MPK3 and MPK6 target a subclass of \u2019VQ-motif\u2019-containing proteins to regulate immune responses","volume":"203","author":"Pecher","year":"2014","journal-title":"New Phytol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s12284-021-00483-y","article-title":"Two VQ Proteins are Substrates of the OsMPKK6-OsMPK4 Cascade in Rice Defense Against Bacterial Blight","volume":"14","author":"Li","year":"2021","journal-title":"Rice"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Uji, Y., Kashihara, K., Kiyama, H., Mochizuki, S., Akimitsu, K., and Gomi, K. (2019). Jasmonic Acid-Induced VQ-Motif-Containing Protein OsVQ13 Influences the OsWRKY45 Signaling Pathway and Grain Size by Associating with OsMPK6 in Rice. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20122917"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Wang, M., Vannozzi, A., Wang, G., Zhong, Y., Corso, M., Cavallini, E., and Cheng, Z.M. (2015). A comprehensive survey of the grapevine VQ gene family and its transcriptional correlation with WRKY proteins. Front. Plant Sci., 6.","DOI":"10.3389\/fpls.2015.00417"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e13411","DOI":"10.1111\/mpp.13411","article-title":"MdVQ12 confers resistance to Valsa mali by regulating MdHDA19 expression in apple","volume":"25","author":"Han","year":"2024","journal-title":"Mol. Plant Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1111\/jipb.70164","article-title":"The VQ motif-containing proteins VQ18 and VQ26 regulate flowering time in Arabidopsis thaliana","volume":"68","author":"Li","year":"2026","journal-title":"J. Integr. Plant Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1104\/pp.112.196816","article-title":"Structural and functional analysis of VQ motif-containing proteins in Arabidopsis as interacting proteins of WRKY transcription factors","volume":"159","author":"Cheng","year":"2012","journal-title":"Plant Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"111235","DOI":"10.1016\/j.celrep.2022.111235","article-title":"A VQ-motif-containing protein fine-tunes rice immunity and growth by a hierarchical regulatory mechanism","volume":"40","author":"Hao","year":"2022","journal-title":"Cell Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1007\/s00299-021-02766-6","article-title":"OsVQ1 links rice immunity and flowering via interaction with a mitogen-activated protein kinase OsMPK6","volume":"40","author":"Wang","year":"2021","journal-title":"Plant Cell Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1093\/mp\/ssu049","article-title":"Growth-defense tradeoffs in plants: A balancing act to optimize fitness","volume":"7","author":"Huot","year":"2014","journal-title":"Mol. Plant"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.tplants.2020.01.004","article-title":"Stories of Salicylic Acid: A Plant Defense Hormone","volume":"25","author":"Ding","year":"2020","journal-title":"Trends Plant Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1007\/s10886-014-0468-3","article-title":"Jasmonate-triggered plant immunity","volume":"40","author":"Campos","year":"2014","journal-title":"J. Chem. Ecol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1093\/jxb\/erv518","article-title":"Ethylene response factors in Arabidopsis immunity","volume":"67","author":"Huang","year":"2016","journal-title":"J. Exp. Bot."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1146\/annurev.phyto.43.040204.135923","article-title":"Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens","volume":"43","author":"Glazebrook","year":"2005","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.tplants.2012.02.010","article-title":"Evolution of jasmonate and salicylate signal crosstalk","volume":"17","author":"Thaler","year":"2012","journal-title":"Trends Plant Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1146\/annurev-phyto-082712-102314","article-title":"MAPK cascades in plant disease resistance signaling","volume":"51","author":"Meng","year":"2013","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Wang, J., Tao, F., Tian, W., Guo, Z., Chen, X., Xu, X., Shang, H., and Hu, X. (2017). The wheat WRKY transcription factors TaWRKY49 and TaWRKY62 confer differential high-temperature seedling-plant resistance to Puccinia striiformis f. sp. tritici. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0181963"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"110716","DOI":"10.1016\/j.plaphy.2025.110716","article-title":"Rapeseed WRKY53 regulates pattern-triggered immunity (PTI) through modulating the transcription of salicylic acid biosynthetic genes","volume":"230","author":"Ma","year":"2026","journal-title":"Plant Physiol. Biochem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.plaphy.2020.12.005","article-title":"The role of plant-specific VQ motif-containing proteins: An ever-thickening plot","volume":"159","author":"Yuan","year":"2021","journal-title":"Plant Physiol. Biochem."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Jiang, S.Y., Sevugan, M., and Ramachandran, S. (2018). Valine-glutamine (VQ) motif coding genes are ancient and non-plant-specific with comprehensive expression regulation by various biotic and abiotic stresses. BMC Genom., 19.","DOI":"10.1186\/s12864-018-4733-7"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/s42003-024-06258-7","article-title":"Structural basis for the regulation of plant transcription factor WRKY33 by the VQ protein SIB1","volume":"7","author":"Dong","year":"2024","journal-title":"Commun. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Li, N., Han, X., Feng, D., Yuan, D., and Huang, L.-J. (2019). Signaling Crosstalk between Salicylic Acid and Ethylene\/Jasmonate in Plant Defense: Do We Understand What They Are Whispering?. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20030671"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1007\/s44154-025-00250-9","article-title":"Linking the interaction of Salicylates and Jasmonates for stress resilience in plants","volume":"5","author":"Pandey","year":"2025","journal-title":"Stress Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e70064","DOI":"10.1111\/mpp.70064","article-title":"MdVQ37 Negatively Regulates Apple Resistance to Valsa Canker via SA-Dependent and SA-Independent Pathways","volume":"26","author":"Duan","year":"2025","journal-title":"Mol. Plant Pathol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"102365","DOI":"10.1016\/j.pmpp.2024.102365","article-title":"Overexpression of pumpkin CpVQ30 increased susceptibility of tobacco to powdery mildew","volume":"133","author":"Li","year":"2024","journal-title":"Physiol. Mol. Plant Pathol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"102562","DOI":"10.1016\/j.pmpp.2025.102562","article-title":"Pumpkin (Cucurbita pepo L.) CpVQ20 increases resistance to powdery mildew of tobacco through flavonoid and lignin biosynthesis pathways","volume":"136","author":"Li","year":"2025","journal-title":"Physiol. Mol. Plant Pathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"393","DOI":"10.4161\/psb.5.4.10793","article-title":"ROS signaling in the hypersensitive response: When, where and what for?","volume":"5","author":"Zurbriggen","year":"2010","journal-title":"Plant Signal. Behav."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1985860","DOI":"10.1080\/15592324.2021.1985860","article-title":"Reactive oxygen species and nitric oxide as mediators in plant hypersensitive response and stomatal closure","volume":"16","author":"Liu","year":"2021","journal-title":"Plant Signal. Behav."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e70562","DOI":"10.1111\/tpj.70562","article-title":"The R2R3-MYB transcription factor GhMYB315 associates with GhVQ28 to enhance Verticillium wilt resistance in upland cotton by regulating phenylpropanoid metabolism","volume":"124","author":"Guo","year":"2025","journal-title":"Plant J."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Lu, Y., and Yao, J. (2018). Chloroplasts at the Crossroad of Photosynthesis, Pathogen Infection and Plant Defense. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19123900"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1111\/pce.14329","article-title":"The WRKY10-VQ8 module safely and effectively regulates rice thermotolerance","volume":"45","author":"Chen","year":"2022","journal-title":"Plant Cell Environ."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/S0014-5793(02)02388-8","article-title":"Novel nuclear-encoded proteins interacting with a plastid sigma factor, Sig1, in Arabidopsis thaliana","volume":"514","author":"Morikawa","year":"2002","journal-title":"FEBS Lett."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"W78","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive Tree of Life (iTOL) v6: Recent updates to the phylogenetic tree display and anno-tation tool","volume":"52","author":"Letunic","year":"2024","journal-title":"Nucleic Acids Res."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/15\/2283\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T04:11:22Z","timestamp":1785211882000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/15\/2283"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,26]]},"references-count":43,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["plants15152283"],"URL":"https:\/\/doi.org\/10.3390\/plants15152283","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,26]]}}}