{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T11:34:29Z","timestamp":1786448069881,"version":"build-2736575974"},"reference-count":16,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018693","name":"HORIZON EUROPE European Innovation Council","doi-asserted-by":"publisher","award":["101060593"],"award-info":[{"award-number":["101060593"]}],"id":[{"id":"10.13039\/100018693","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The thermotherapy technique has been used to evaluate the viability of hot water treatments (HWTs) for the elimination of different pathogens that infect commercial fruit trees. The present project focuses on common pathogens of Prunus species at the nursery level when external propagation material is needed, which is not always in optimal sanitary conditions. Different combinations of temperature\/duration treatments were applied to budwood of nectarines infected by Xanthomonas arboricola pv. pruni (Xap) and almonds infected by Prunus necrotic ringspot virus (PNRSV). These treated buds were then grafted onto disease-free micropropagated rootstocks. In bacteria-infected nectarine tissues treated at 45 \u00b0C for 40 min, Xap was not detected in any sample, preserving a high viability of the grafted plants. In virus-infected almond tissues treated at 45 \u00b0C for 50 min a few individual plants were identified in which no virus was detected. These results demonstrated the effectiveness of the HWT and suggest that these techniques could be applied at nursery level, on a large scale for the eradication of bacteria or on a small scale for the recovery of virus-free individuals.<\/jats:p>","DOI":"10.3390\/plants15162440","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T10:32:16Z","timestamp":1786444336000},"page":"2440","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Thermotherapy Treatments of Fruit Trees of the Genus Prunus Infected by Viruses and Bacteria"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4703-0165","authenticated-orcid":false,"given":"Nat\u00e0lia","family":"Puig-Gay","sequence":"first","affiliation":[{"name":"Agromillora Iberia SLU, El Rebato s\/n, Subirats, 08739 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Rieder","sequence":"additional","affiliation":[{"name":"Agromillora Iberia SLU, El Rebato s\/n, Subirats, 08739 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0378-9735","authenticated-orcid":false,"given":"Mireia","family":"Bordas","sequence":"additional","affiliation":[{"name":"Agromillora Iberia SLU, El Rebato s\/n, Subirats, 08739 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (2015). 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Virol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1094\/PDIS.2003.87.11.1344","article-title":"Development of Real-Time RT-PCR Assay for Detection of Prunus Necrotic Ringspot Virus in Fruit Trees","volume":"87","author":"Marbot","year":"2007","journal-title":"Plant Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1128\/AEM.01593-10","article-title":"Development of an Efficient Real-Time Quantitative PCR Protocol for Detection of Xanthomonas Arboricola Pv. Pruni in Prunus Species","volume":"77","author":"Cubero","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1111\/mpp.12679","article-title":"Xanthomonas Arboricola Pv. Pruni, Causal Agent of Bacterial Spot of Stone Fruits and Almond: Its Genomic and Phenotypic Characteristics in the X. Arboricola Species Context","volume":"19","author":"Cubero","year":"2018","journal-title":"Mol. Plant Pathol."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2440\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T10:58:33Z","timestamp":1786445913000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2440"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,11]]},"references-count":16,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["plants15162440"],"URL":"https:\/\/doi.org\/10.3390\/plants15162440","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,11]]}}}