{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T04:30:18Z","timestamp":1786681818102,"version":"build-2736575974"},"reference-count":40,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32460356"],"award-info":[{"award-number":["32460356"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32460356"],"award-info":[{"award-number":["32460356"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The bud bank is the reproductive body bank of grassland plants, which plays a key role in population maintenance and renewal. To study the effects of rest grazing on underground bud bank density, the changes in bud bank density were clarified to provide reference for grassland grazing management. In this experiment, according to the soil thawing and forage grass regreening in spring, the time when the cold-season pasture began to rest was determined. The period of grass withering is the end time of rest grazing. Different grazing rest periods are as follows: critical period of soil thawing\u2013plant withering (RP1), early period of grass revival\u2013plant withering (RP2) and period of local traditional rest grazing\u2013plant withering (RP3). We studied the effects of different periods of rest grazing on the density and composition of underground bud banks. The results showed that the total density of underground bud banks increased by 33%, and the density of tiller buds, rhizome buds and root tiller buds increased by 9~62% compared with RP3. The proportion of different buds in different plots was tiller bud &gt; rhizome bud &gt; root tiller bud &gt; bulb bud. Compared with RP3, the tiller bud density in RP1 increased by 24%. In RP1, the aboveground biomass and branch density of plants in RP1 were significantly higher than those in RP3 (p &lt; 0.05), which were 56% and 29% higher than those in RP3, respectively. Finally, it is concluded that grazing rest before soil thawing in spring alpine meadow can promote the increase in plant underground bud bank density and the accumulation of aboveground biomass.<\/jats:p>","DOI":"10.3390\/plants15162449","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T07:57:26Z","timestamp":1786521446000},"page":"2449","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Spring Rest Grazing on Underground Bud Bank in Alpine Meadow of Tibetan Plateau Before Critical Period of Soil Thawing"],"prefix":"10.3390","volume":"15","author":[{"given":"Meimei","family":"Bai","sequence":"first","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem, Ministry of Education\/Sino, U.S. Center for Grazing Land, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaochong","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem, Ministry of Education\/Sino, U.S. Center for Grazing Land, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changlin","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem, Ministry of Education\/Sino, U.S. Center for Grazing Land, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem, Ministry of Education\/Sino, U.S. Center for Grazing Land, College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"ref_1","first-page":"139","article-title":"Analysis of current grassland resources in China","volume":"61","author":"Shen","year":"2016","journal-title":"Chin. Sci. Bull."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Dong, S.K., Zhang, Y., Shen, H., Li, S., and Xu, Y.D. (2023). Overview of the third pole\u2019s grasslands. Grasslands on the Third Pole of the World: Structure, Function, Process, and Resilience of Social-Ecological Systems, Springer International Publishing.","DOI":"10.1007\/978-3-031-39485-0"},{"key":"ref_3","unstructured":"Chen, B. (2023). Regulatory Mechanisms Under Alpine Grasslands Sensitivity to Climate Change on the Qinghai-Tibetan Plateau. [Master\u2019s Thesis, Hebei Normal University]."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"108929","DOI":"10.1016\/j.ecolind.2022.108929","article-title":"Advancing the spring rest-grazing time until the critical period when soil thaws promotes soil recovery and bacterial diversity in alpine meadows","volume":"139","author":"Jing","year":"2022","journal-title":"Ecol. Indic."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.ppees.2006.10.002","article-title":"Bud banks and their role in vegetative regeneration-a literature review and proposal for simple classification and assessment","volume":"8","year":"2007","journal-title":"Perspect. Plant Ecol. Evol. Syst."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1093\/aob\/mcz051","article-title":"The ecology and significance of below-ground bud banks in plants","volume":"123","author":"Ott","year":"2019","journal-title":"Ann. Bot."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.3732\/ajb.1500039","article-title":"Bud-bank and tiller dynamics of co-occurring C3 caespitose grasses in mixed-grass prairie","volume":"102","author":"Ott","year":"2015","journal-title":"Am. J. Bot."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"He, K.Y., Zhou, Q.P., Dang, H.H., Wang, X.L., He, L.L., Wei, X.X., Li, J.Y., Wang, Q., and Wang, J.H. (2026). Soil-mediated regulatory mechanisms of belowground bud banks in the sustainable management and ecological restoration of degraded alpine grasslands. Sustainability, 18.","DOI":"10.3390\/su18020572"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"rtae029","DOI":"10.1093\/jpe\/rtae029","article-title":"Responses of belowground bud bank density of geophytes to environmental perturbations: A meta-analysis","volume":"17","author":"Fang","year":"2024","journal-title":"J. Plant Ecol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"110189","DOI":"10.1016\/j.agee.2025.110189","article-title":"Litter manipulation alters the belowground bud bank and aboveground shoot density in a temperate grassland","volume":"399","author":"Qian","year":"2026","journal-title":"Agric. Ecosyst. Environ."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"672","DOI":"10.3732\/ajb.2007277","article-title":"Responses of two bunchgrasses to nitrogen addition in tallgrass prairie: The role of bud bank demography","volume":"95","author":"Dalgleish","year":"2008","journal-title":"Am. J. Bot."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1002\/ldr.4522","article-title":"Temperature-related factors are the better determinants of belowground bud density, while moisture-related factors are the better determinants of belowground bud diversity at the regional scale","volume":"34","author":"Ma","year":"2023","journal-title":"Land Degrad. Dev."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ding, X.J., Su, P.X., Zhou, Z.J., and Shi, R. (2019). Belowground bud bank distribution and aboveground community characteristics along different moisture gradients of alpine meadow in the Zoige Plateau, China. Sustainability, 11.","DOI":"10.3390\/su11092602"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Li, Y., Bao, G.S., Zhang, P., Feng, X.Y., Ma, J.J., Lu, H.N., Shi, H.X., Wei, X.X., Tang, B.M., and Liu, K. (2023). Changes in bud bank and their correlation with plant community composition in degraded alpine meadows. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1259340"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"421","DOI":"10.2478\/v10183-012-0019-y","article-title":"Clonal growth forms in Arctic plants and their habitat preferences: A study from Petuniabukta, Spitsbergen","volume":"33","author":"Prach","year":"2012","journal-title":"Pol. Polar Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2791","DOI":"10.1002\/ecy.1500","article-title":"Can trait patterns along gradients predict plant community responses to climate change?","volume":"97","author":"Guittar","year":"2016","journal-title":"Ecology"},{"key":"ref_17","unstructured":"Zhang, J.F. (2017). Responses of Bud Bank Dynamics of Nitraria tangutorum to Nitrogen Addition. [Master\u2019s Thesis, Chinese Academy of Forestry]."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1007\/s10682-007-9195-3","article-title":"Clonal splitters and integrators in harsh environments of the Trans-Himalaya","volume":"22","year":"2008","journal-title":"Evol. Ecol."},{"key":"ref_19","first-page":"404","article-title":"Bud population dynamics of Phragmites australis in heterogeneous habitats of Northeast grassland, China","volume":"26","author":"Jiao","year":"2015","journal-title":"Chin. J. Appl. Ecol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1126\/science.aaz7614","article-title":"The physiology of plant responses to drought","volume":"368","author":"Gupta","year":"2020","journal-title":"Science"},{"key":"ref_21","unstructured":"Jing, Y.Y. (2023). Effects of Rest-Grazing on Soil Carbon Pool and Carbon Components in Cold Season Pasture of Alpine Meadow. [Doctoral Thesis, Gansu Agricultural University]."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"106634","DOI":"10.1016\/j.ecoleng.2022.106634","article-title":"Rest grazing from the critical period of soil thawing promotes the propagation of Kobresia humilis in alpine meadow","volume":"179","author":"Bai","year":"2022","journal-title":"Ecol. Eng."},{"key":"ref_23","unstructured":"Peng, Z. (2020). Effects of Rest Grazing in Different Stages of Spring on Reproduction and Root of Kobresia capillifolia and Elymus nutans in Alpine Meadow. [Master\u2019s Thesis, Gansu Agricultural University]."},{"key":"ref_24","unstructured":"Ma, K.K. (2023). Effects of Different Rest Grazing Periods on Soil Seed Bank, Plant Community Characteristics and Theoretical Grazing Capacity in Alpine meadow. [Master\u2019s Thesis, Gansu Agricultural University]."},{"key":"ref_25","unstructured":"Shang, Z.H. (2006). Studies on Soil Seed Bank and Regeneration of Degraded Alpine Grassland in the Headwaters of Yangtze and Yellow Rivers on Tibetan Plateau. [Doctoral Thesis, Gansu Agricultural University]."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1890\/09-0981.1","article-title":"Restoring diversity after cattail expansion: Disturbance, resilience and seasonality in a tropical dry wetland","volume":"21","author":"Osland","year":"2011","journal-title":"Ecol. Appl."},{"key":"ref_27","first-page":"571","article-title":"Limiting factors of degraded grassland restoration in China and related basic scientific issues","volume":"37","author":"Pan","year":"2023","journal-title":"Bull. Natl. Nat. Sci. Found. China"},{"key":"ref_28","first-page":"24","article-title":"Soil seed bank characteristics of typical plant communities in alpine Kobresia meadows under grazing intensities","volume":"45","author":"Li","year":"2025","journal-title":"Grassl. Turf"},{"key":"ref_29","first-page":"9","article-title":"Research progress of clonal plant ecology and its significance in ecological restoration","volume":"1","author":"Li","year":"2017","journal-title":"Contemp. Hortic."},{"key":"ref_30","unstructured":"Zhang, J.T. (2009). The Variation of All Types of Buds of Belowground Bud Bank and Their Relationship with Aboveground Shoots in Leymus chinensis Population. [Doctoral Thesis, Northeast Normal University]."},{"key":"ref_31","unstructured":"Bai, X. (2017). Effects of Enclosure Duration on Offspring Recruitment and Bud Bank in Semiarid Steppe on the Loess Plateau. [Master\u2019s Thesis, Henan University of Science and Technology]."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s11258-005-0975-y","article-title":"The role of seed and vegetative reproduction in plant recruitment and demography in tallgrass prairie","volume":"187","author":"Benson","year":"2006","journal-title":"Plant Ecol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"387","DOI":"10.3170\/2008-7-18487","article-title":"Plant traits and regeneration of urban plant communities after disturbance: Does the bud bank play any role?","volume":"11","author":"Latzel","year":"2008","journal-title":"Appl. Veg. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1111\/1365-2435.12731","article-title":"Disentangling the interplay of generative and vegetative propagation among different functional groups during gap colonization in meadows","volume":"31","author":"Macek","year":"2017","journal-title":"Funct. Ecol."},{"key":"ref_35","unstructured":"Soil Survey Staff (2003). Keys to Soil Taxonomy."},{"key":"ref_36","unstructured":"Xu, P. (2000). Grassland Resource Inventory and Planning, China Agriculture Press."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1093\/aob\/mcx072","article-title":"Differences in below-ground bud bank density and composition along a climatic gradient in the temperate steppe of northern China","volume":"120","author":"Qian","year":"2017","journal-title":"Ann. Bot."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"107520","DOI":"10.1016\/j.ecolind.2021.107520","article-title":"Belowground bud bank and its relationship with aboveground vegetation under watering and nitrogen addition in temperate semiarid steppe","volume":"125","author":"Qian","year":"2021","journal-title":"Ecol. Indic."},{"key":"ref_39","first-page":"755","article-title":"Effects of fertilization timing on belowground bud bank and its relationship with vegetation of degraded meadow steppe","volume":"44","author":"Tao","year":"2025","journal-title":"Chin. J. Ecol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"416","DOI":"10.3732\/ajb.91.3.416","article-title":"Belowground bud banks and meristem limitation in tallgrass prairie plant populations","volume":"91","author":"Benson","year":"2004","journal-title":"Am. J. Bot."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2449\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T04:16:51Z","timestamp":1786681011000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2449"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,12]]},"references-count":40,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["plants15162449"],"URL":"https:\/\/doi.org\/10.3390\/plants15162449","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,12]]}}}