{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T04:29:49Z","timestamp":1786768189931,"version":"build-2736575974"},"reference-count":76,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"crossref","award":["262300421488"],"award-info":[{"award-number":["262300421488"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372239"],"award-info":[{"award-number":["32372239"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32372239"],"award-info":[{"award-number":["32372239"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Nitrous oxide (N2O) is a potent greenhouse gas and a major ozone-depleting substance of the nitrogen cycle. While global N2O budgets focus on microbial soil sources, evidence suggests that living plants can also contribute to N2O emissions. However, the origin of plant-associated N2O fluxes is contested: measured emissions may arise from endogenous plant metabolism or merely from transport of soil-derived N2O. In this review, we clarify these pathways and synthesize current findings from laboratory and field studies. Experimental evidence from 15N-tracer and axenic-culture studies supports N2O formation linked to nitrate (NO3\u2212) and nitrite (NO2\u2212) in photosynthetic organisms, although mechanistic resolution varies among taxa. In angiosperms, proposed plastid\/chloroplast and hypoxia-associated mitochondrial routes are linked to NO3\u2212\/NO2\u2212 metabolism and NO formation, but the terminal NO-to-N2O step remains unresolved and differs from the flavodiiron-dependent mechanism demonstrated in algae. We discuss key environmental and physiological controls (substrate availability, light, O2 status, reductant supply) on these processes and highlight methodological challenges in source attribution (chamber artefacts, destructive sampling, isotope analysis). Our analysis suggests that plant-associated N2O fluxes are real, but highly variable and context-dependent. Rather than assuming generic \u201cplant emission factors\u201d, we emphasize source-specific approaches that distinguish endogenous formation from soil-derived transport and microbial production. This framework can improve N2O budgets and guide mitigation by indicating whether management should target soil N availability and microbial processes or physiological conditions favouring plant-associated N2O formation.<\/jats:p>","DOI":"10.3390\/plants15162460","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T09:31:09Z","timestamp":1786613469000},"page":"2460","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Endogenous Plant Nitrous Oxide Formation: Evidence, Mechanisms and Ecosystem Implications"],"prefix":"10.3390","volume":"15","author":[{"given":"Siddique","family":"Ahmad","sequence":"first","affiliation":[{"name":"College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Song","sequence":"additional","affiliation":[{"name":"College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyang","family":"Song","sequence":"additional","affiliation":[{"name":"College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shabnam","family":"Hadi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengdi","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siying","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laiba","family":"Urooj","sequence":"additional","affiliation":[{"name":"College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuping","family":"Xiong","sequence":"additional","affiliation":[{"name":"College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochun","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqiang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinming","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9897-0571","authenticated-orcid":false,"given":"Yihao","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1126\/science.1176985","article-title":"Nitrous Oxide (N2O): The Dominant Ozone-Depleting Substance Emitted in the 21st Century","volume":"326","author":"Ravishankara","year":"2009","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1098\/rstb.2011.0415","article-title":"Biological Sources and Sinks of Nitrous Oxide and Strategies to Mitigate Emissions","volume":"367","author":"Thomson","year":"2012","journal-title":"Phil. Trans. R. Soc. B"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.5194\/essd-16-2543-2024","article-title":"Global Nitrous Oxide Budget (1980\u20132020)","volume":"16","author":"Tian","year":"2024","journal-title":"Earth Syst. Sci. Data"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"20130122","DOI":"10.1098\/rstb.2013.0122","article-title":"Nitrous Oxide Emissions from Soils: How Well Do We Understand the Processes and Their Controls?","volume":"368","author":"Baggs","year":"2013","journal-title":"Phil. Trans. R. Soc. B"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1038\/nrmicro.2018.9","article-title":"The Microbial Nitrogen-Cycling Network","volume":"16","author":"Kuypers","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"35","DOI":"10.2136\/sssaj1998.03615995006200010005x","article-title":"Nitrous Oxide Emission through Plants","volume":"62","author":"Chang","year":"1998","journal-title":"Soil Sci. Soc. Am. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s003740050510","article-title":"Emission of N2O from Rye Grass (Lolium perenne L.)","volume":"28","author":"Chen","year":"1999","journal-title":"Biol. Fertil. Soils"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"175115","DOI":"10.1016\/j.scitotenv.2024.175115","article-title":"Plants Mitigate Ecosystem Nitrous Oxide Emissions Primarily through Reductions in Soil Nitrate Content: Evidence from a Meta-Analysis","volume":"949","author":"Timilsina","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7875","DOI":"10.1073\/pnas.131572798","article-title":"Wheat Leaves Emit Nitrous Oxide during Nitrate Assimilation","volume":"98","author":"Smart","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1111\/nph.15455","article-title":"Nitrous Oxide Effluxes from Plants as a Potentially Important Source to the Atmosphere","volume":"221","author":"Lenhart","year":"2019","journal-title":"New Phytol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Timilsina, A., Zhang, C., Pandey, B., Bizimana, F., Dong, W., and Hu, C. (2020). Potential Pathway of Nitrous Oxide Formation in Plants. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.01177"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"150262","DOI":"10.1016\/j.scitotenv.2021.150262","article-title":"Plants Are a Natural Source of Nitrous Oxide Even in Field Conditions as Explained by 15N Site Preference","volume":"805","author":"Timilsina","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1104\/pp.82.3.718","article-title":"Nitric Oxide and Nitrous Oxide Production by Soybean and Winged Bean during the in Vivo Nitrate Reductase Assay","volume":"82","author":"Dean","year":"1986","journal-title":"Plant Physiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1046\/j.1365-313X.1999.00494.x","article-title":"Emission of Nitrous Oxide (N2O) from Transgenic Tobacco Expressing Antisense NiR mRNA","volume":"19","author":"Goshima","year":"1999","journal-title":"Plant J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1111\/j.1469-8137.2005.01542.x","article-title":"Plant-mediated Nitrous Oxide Emissions from Beech (Fagus sylvatica) Leaves","volume":"168","author":"Pihlatie","year":"2005","journal-title":"New Phytol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s11104-015-2629-8","article-title":"Nitrous Oxide Emissions from Stems of Ash (Fraxinus angustifolia Vahl) and European Beech (Fagus aylvatica L.)","volume":"398","author":"Heras","year":"2016","journal-title":"Plant Soil"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4989","DOI":"10.1038\/s41467-019-12976-y","article-title":"Seasonal Dynamics of Stem N2O Exchange Follow the Physiological Activity of Boreal Trees","volume":"10","author":"Machacova","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6737","DOI":"10.5194\/bg-10-6737-2013","article-title":"Nitrous Oxide (N2O) Production in Axenic Chlorella Vulgaris Microalgae Cultures: Evidence, Putative Pathways, and Potential Environmental Impacts","volume":"10","author":"Guieysse","year":"2013","journal-title":"Biogeosciences"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/tpj.13544","article-title":"The Biosynthesis of Nitrous Oxide in the Green Alga Chlamydomonas reinhardtii","volume":"91","author":"Plouviez","year":"2017","journal-title":"Plant J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2704","DOI":"10.1073\/pnas.1915276117","article-title":"Algal Photosynthesis Converts Nitric Oxide into Nitrous Oxide","volume":"117","author":"Burlacot","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Bellido-Pedraza, C.M., Calatrava, V., Llamas, A., Fernandez, E., Sanz-Luque, E., and Galvan, A. (2022). Nitrous Oxide Emissions from Nitrite Are Highly Dependent on Nitrate Reductase in the Microalga Chlamydomonas reinhardtii. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23169412"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1111\/nph.14536","article-title":"Alternative Electron Transport Mediated by Flavodiiron Proteins Is Operational in Organisms from Cyanobacteria up to Gymnosperms","volume":"214","author":"Alboresi","year":"2017","journal-title":"New Phytol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/s11104-012-1359-4","article-title":"Inundation Strongly Stimulates Nitrous Oxide Emissions from Stems of the Upland Tree Fagus Sylvatica and the Riparian Tree Alnus Glutinosa","volume":"364","author":"Machacova","year":"2013","journal-title":"Plant Soil"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e17181","DOI":"10.1111\/gcb.17181","article-title":"Foliar N2O Emissions Constitute a Significant Source to Atmosphere","volume":"30","author":"Qin","year":"2024","journal-title":"Glob. Change Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1093\/femsre\/fuv021","article-title":"Microbial Regulation of Terrestrial Nitrous Oxide Formation: Understanding the Biological Pathways for Prediction of Emission Rates","volume":"39","author":"Hu","year":"2015","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/s41586-020-2780-0","article-title":"A Comprehensive Quantification of Global Nitrous Oxide Sources and Sinks","volume":"586","author":"Tian","year":"2020","journal-title":"Nature"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1093\/jxb\/eri252","article-title":"In Higher Plants, Only Root Mitochondria, but Not Leaf Mitochondria Reduce Nitrite to NO, in Vitro and in Situ","volume":"56","author":"Gupta","year":"2005","journal-title":"J. Exp. Bot."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1111\/j.1399-3054.2009.01314.x","article-title":"Anoxic Nitric Oxide Cycling in Plants: Participating Reactions and Possible Mechanisms","volume":"138","author":"Igamberdiev","year":"2010","journal-title":"Physiol. Plant."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"23410","DOI":"10.1038\/srep23410","article-title":"Pinus Sylvestris as a Missing Source of Nitrous Oxide and Methane in Boreal Forest","volume":"6","author":"Machacova","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1111\/pce.12739","article-title":"A Dual System Formed by the ARC and NR Molybdoenzymes Mediates Nitrite-dependent NO Production in Chlamydomonas","volume":"39","author":"Llamas","year":"2016","journal-title":"Plant Cell Environ."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1002\/abio.200390032","article-title":"Conversion of the Nitrate Nitrogen and Nitrogen Dioxide to Nitrous Oxides in Plants","volume":"23","author":"Hakata","year":"2003","journal-title":"Acta Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1128\/AEM.72.1.638-644.2006","article-title":"Distinguishing Nitrous Oxide Production from Nitrification and Denitrification on the Basis of Isotopomer Abundances","volume":"72","author":"Sutka","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.1002\/rcm.3820","article-title":"Isotopologue Fractionation during N2O Production by Fungal Denitrification","volume":"22","author":"Sutka","year":"2008","journal-title":"Rapid Comm. Mass. Spectrom."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1002\/rcm.3456","article-title":"A Review of Stable Isotope Techniques for N2O Source Partitioning in Soils: Recent Progress, Remaining Challenges and Future Considerations","volume":"22","author":"Baggs","year":"2008","journal-title":"Rapid Comm. Mass. Spectrom."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/mas.21459","article-title":"Isotopocule Analysis of Biologically Produced Nitrous Oxide in Various Environments","volume":"36","author":"Toyoda","year":"2017","journal-title":"Mass Spectrom. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.tplants.2016.12.001","article-title":"Nitrate Reductase Regulates Plant Nitric Oxide Homeostasis","volume":"22","author":"Llamas","year":"2017","journal-title":"Trends Plant Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.mito.2011.03.005","article-title":"The Anoxic Plant Mitochondrion as a Nitrite: NO Reductase","volume":"11","author":"Gupta","year":"2011","journal-title":"Mitochondrion"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3401","DOI":"10.1093\/jxb\/erx420","article-title":"Nitric Oxide Production in Plants: An Update","volume":"69","author":"Astier","year":"2018","journal-title":"J. Exp. Bot."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1093\/jexbot\/53.366.103","article-title":"Regulation of Nitric Oxide (NO) Production by Plant Nitrate Reductase In Vivo and In Vitro","volume":"53","author":"Rockel","year":"2002","journal-title":"J. Exp. Bot."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1093\/pcp\/pcf034","article-title":"Nitric Oxide Production Mediated by Nitrate Reductase in the Green Alga Chlamydomonas Reinhardtii: An Alternative NO Production Pathway in Photosynthetic Organisms","volume":"43","author":"Sakihama","year":"2002","journal-title":"Plant Cell Physiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/BF00390226","article-title":"Nitrite Reduction in Leaves; Studies on Isolated Chloroplasts","volume":"116","author":"Miflin","year":"1974","journal-title":"Planta"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Sanz-Luque, E., Chamizo-Ampudia, A., Llamas, A., Galvan, A., and Fernandez, E. (2015). Understanding Nitrate Assimilation and Its Regulation in Microalgae. Front. Plant Sci., 6.","DOI":"10.3389\/fpls.2015.00899"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1007\/s00425-002-0816-3","article-title":"Nitrite Accumulation and Nitric Oxide Emission in Relation to Cellular Signaling in Nitrite Reductase Antisense Tobacco","volume":"215","author":"Rockel","year":"2002","journal-title":"Planta"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Sun, H., Feng, F., Liu, J., and Zhao, Q. (2018). Nitric Oxide Affects Rice Root Growth by Regulating Auxin Transport Under Nitrate Supply. Front. Plant Sci., 9.","DOI":"10.3389\/fpls.2018.00659"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1104\/pp.106.086918","article-title":"Chloroplasts as a Nitric Oxide Cellular Source. Effect of Reactive Nitrogen Species on Chloroplastic Lipids and Proteins","volume":"142","author":"Jasid","year":"2006","journal-title":"Plant Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Yang, H., Zhou, Y., Zhang, Y., Wang, J., and Shi, H. (2019). Identification of Transcription Factors of Nitrate Reductase Gene Promoters and NRE2 Cis-Element through Yeast One-Hybrid Screening in Nicotiana Tabacum. BMC Plant Biol., 19.","DOI":"10.1186\/s12870-019-1724-z"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Gomez-Osuna, A., Calatrava, V., Galvan, A., Fernandez, E., and Llamas, A. (2020). Identification of the MAPK Cascade and Its Relationship with Nitrogen Metabolism in the Green Alga Chlamydomonas reinhardtii. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21103417"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"10765","DOI":"10.1021\/bi048826r","article-title":"Reactions of Spinach Nitrite Reductase with Its Substrate, Nitrite, and a Putative Intermediate, Hydroxylamine","volume":"43","author":"Kuznetsova","year":"2004","journal-title":"Biochemistry"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2593","DOI":"10.1093\/jxb\/erw507","article-title":"How Chlamydomonas Handles Nitrate and the Nitric Oxide Cycle","volume":"68","author":"Calatrava","year":"2017","journal-title":"J. Exp. Bot."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.cmet.2006.02.011","article-title":"Mitochondrial Cytochrome Oxidase Produces Nitric Oxide under Hypoxic Conditions: Implications for Oxygen Sensing and Hypoxic Signaling in Eukaryotes","volume":"3","author":"Castello","year":"2006","journal-title":"Cell Metab."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1093\/pcp\/pcq022","article-title":"Production and Scavenging of Nitric Oxide by Barley Root Mitochondria","volume":"51","author":"Gupta","year":"2010","journal-title":"Plant Cell Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1104\/pp.110.166140","article-title":"Both Plant and Bacterial Nitrate Reductases Contribute to Nitric Oxide Production in Medicago Truncatula Nitrogen-Fixing Nodules","volume":"155","author":"Horchani","year":"2011","journal-title":"Plant Physiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1016\/j.bbabio.2008.06.002","article-title":"Nitrite\u2013Nitric Oxide Control of Mitochondrial Respiration at the Frontier of Anoxia","volume":"1777","author":"Benamar","year":"2008","journal-title":"Biochim. Biophys. Acta (BBA)-Bioenerg."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1006\/bbrc.1995.2076","article-title":"Cytochrome c Oxidase Catalysis of the Reduction of Nitric Oxide to Nitrous Oxide","volume":"212","author":"Zhao","year":"1995","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5275","DOI":"10.1021\/bi00564a020","article-title":"Reactions of Nitric Oxide with Cytochrome c Oxidase","volume":"19","author":"Brudvig","year":"1980","journal-title":"Biochemistry"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"5656","DOI":"10.1111\/febs.16450","article-title":"Characterization of the Amidoxime Reducing Components ARC1 and ARC2 from Arabidopsis Thaliana","volume":"289","author":"Maiber","year":"2022","journal-title":"FEBS J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.atmosenv.2014.09.077","article-title":"UV-Induced N2O Emission from Plants","volume":"99","author":"Bruhn","year":"2014","journal-title":"Atmos. Environ."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s11240-018-1415-8","article-title":"Effects of Different LED Sources on the Growth and Nitrogen Metabolism of Lettuce","volume":"134","author":"Zhang","year":"2018","journal-title":"Plant Cell Tiss. Organ Cult."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1002\/pei3.10015","article-title":"N2O Emissions from Plants Are Reduced under Photosynthetic Activity","volume":"1","author":"Meurer","year":"2020","journal-title":"Plant Environ. Interact."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1093\/mp\/ssq049","article-title":"Metabolic and Signaling Aspects Underpinning the Regulation of Plant Carbon Nitrogen Interactions","volume":"3","author":"Fernie","year":"2010","journal-title":"Mol. Plant"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Yoneyama, T., and Suzuki, A. (2020). Light-Independent Nitrogen Assimilation in Plant Leaves: Nitrate Incorporation into Glutamine, Glutamate, Aspartate, and Asparagine Traced by 15N. Plants, 9.","DOI":"10.3390\/plants9101303"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"103916","DOI":"10.1016\/j.envexpbot.2019.103916","article-title":"Comparative Morphological and Transcriptomic Responses of Lowland and Upland Rice to Root-Zone Hypoxia","volume":"169","author":"Liu","year":"2020","journal-title":"Environ. Exp. Bot."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1046\/j.1365-313X.2003.01846.x","article-title":"Expression of a Stress-induced Hemoglobin Affects NO Levels Produced by Alfalfa Root Cultures under Hypoxic Stress","volume":"35","author":"Dordas","year":"2003","journal-title":"Plant J."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1111\/pce.12884","article-title":"The Occurrence and Control of Nitric Oxide Generation by the Plant Mitochondrial Electron Transport Chain","volume":"40","author":"Alber","year":"2017","journal-title":"Plant Cell Environ."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1104\/pp.106.091686","article-title":"S-Nitrosoglutathione Reductase Affords Protection against Pathogens in Arabidopsis, Both Locally and Systemically","volume":"143","author":"Espunya","year":"2007","journal-title":"Plant Physiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"4120","DOI":"10.1105\/tpc.107.055061","article-title":"S-Nitrosylation of Peroxiredoxin II E Promotes Peroxynitrite-Mediated Tyrosine Nitration","volume":"19","author":"Laxa","year":"2008","journal-title":"Plant Cell"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Xu, L., Song, J.-Q., Wang, Y.-L., Liu, X.-H., Li, X.-L., Zhang, B., Li, A.-J., Ye, X.-F., Wang, J., and Wang, P. (2022). Thymol Improves Salinity Tolerance of Tobacco by Increasing the Sodium Ion Efflux and Enhancing the Content of Nitric Oxide and Glutathione. BMC Plant Biol., 22.","DOI":"10.1186\/s12870-021-03395-7"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"331","DOI":"10.2136\/sssaj2007.0215","article-title":"Chamber Measurements of Soil Nitrous Oxide Flux: Are Absolute Values Reliable?","volume":"72","author":"Rochette","year":"2008","journal-title":"Soil Sci. Soc. Am. J."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1002\/jeq2.20117","article-title":"Global Research Alliance N2O Chamber Methodology Guidelines: Design Considerations","volume":"49","author":"Clough","year":"2020","journal-title":"J. Environ. Qual."},{"key":"ref_70","first-page":"1","article-title":"Soils, a Sink for N2O? A Review","volume":"13","author":"Wrage","year":"2007","journal-title":"Glob. Change Biol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1007\/s00425-003-1178-1","article-title":"Nitric Oxide Is Induced by Wounding and Influences Jasmonic Acid Signaling in Arabidopsis Thaliana","volume":"218","author":"Huang","year":"2004","journal-title":"Planta"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"145861","DOI":"10.1016\/j.scitotenv.2021.145861","article-title":"Alien Plant Introductions and Greenhouse Gas Emissions: Insights from Gunnera Tinctoria Invasions","volume":"775","author":"Mantoani","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s11104-008-9673-6","article-title":"Quantification of N2O Fluxes from Soil\u2013Plant Systems May Be Biased by the Applied Gas Chromatograph Methodology","volume":"311","author":"Zheng","year":"2008","journal-title":"Plant Soil"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1002\/jeq2.20124","article-title":"Global Research Alliance N2O Chamber Methodology Guidelines: Considerations for Automated Flux Measurement","volume":"49","author":"Grace","year":"2020","journal-title":"J. Environ. Qual."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1002\/jeq2.20118","article-title":"Global Research Alliance N2O Chamber Methodology Guidelines: Flux Calculations","volume":"49","author":"Venterea","year":"2020","journal-title":"J. Environ. Qual."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1002\/jeq2.20129","article-title":"Global Research Alliance N2O Chamber Methodology Guidelines: Introduction, with Health and Safety Considerations","volume":"49","author":"Harvey","year":"2020","journal-title":"J. Environ. 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