{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T04:26:38Z","timestamp":1786854398836,"version":"build-2736575974"},"reference-count":51,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"INCT\u2014Microorganisms in Agriculture\u2014MicroAgro","award":["CNPq 408267\/2024-0"],"award-info":[{"award-number":["CNPq 408267\/2024-0"]}]},{"name":"INCT\u2014Microorganisms in Agriculture\u2014MicroAgro","award":["158\/2026"],"award-info":[{"award-number":["158\/2026"]}]},{"DOI":"10.13039\/501100022297","name":"Embrapa","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100022297","id-type":"DOI","asserted-by":"crossref"}]},{"name":"CNPq Edital Universal Project","award":["409801\/2023-2"],"award-info":[{"award-number":["409801\/2023-2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Brazilian cattle production relies predominantly on pasture systems, many of which are degraded by nutrient depletion, particularly nitrogen deficiency. Plant growth-promoting bacteria (PGPB) offer a sustainable strategy to improve pasture productivity, but their effects are often host-specific and remain poorly explored in Paspalum. This study evaluated the effect of inoculation with Azospirillum brasilense strains CNPSo 2083 (=Ab-V5) and CNPSo 2084 (=Ab-V6) and Pseudomonas fluorescens strain CNPSo 2719 (=CCTB 03), applied by seed inoculation or leaf spray, on the root morphology and shoot growth of four Paspalum accessions under controlled axenic greenhouse conditions, evaluated in an early growth stage at 35 days after emergence. Genotype-specific responses were the central finding of this study: Paspalum malacophyllum BGP-293 showed negligible responses to inoculation, whereas BGP-486\u2014of the same species\u2014increased root dry weight by up to 43% and root tissue density by up to 30% in all inoculated treatments. In Paspalum regnellii BGP-112, seed inoculation produced stronger root responses than foliar spray, increasing root dry weight by up to 38% and root area by up to 40%. In Paspalum rojasii BGP-149, inoculation induced root architectural remodeling without increasing root biomass\u2014root area increased by up to 92% and total root length by up to 97%, relative to the control\u2014indicating an efficient root-foraging strategy. P. fluorescens favored root-foraging traits and tissue density, whereas A. brasilense preferentially stimulated root-hair elongation. These accession-level contrasts, rather than a single generalizable trend, are the main contribution of this work: they support tailored, genotype-specific PGPB strategies for sustainable tropical pastures.<\/jats:p>","DOI":"10.3390\/plants15162465","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:08:29Z","timestamp":1786694909000},"page":"2465","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genotype-Specific Root System Remodeling of Paspalum spp. Induced by Plant Growth-Promoting Bacteria Enhances the Potential for Sustainable Tropical Pastures"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1876-4936","authenticated-orcid":false,"given":"Beatriz Cortellini","family":"Ferranti","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina, PR-445, km 380, P.O. Box 10.011, Londrina 86057-970, Paran\u00e1, Brazil"},{"name":"Soil Biotechnology Laboratory, Embrapa Soja, P.O. Box 4006, Londrina 86085-981, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Silva","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina, PR-445, km 380, P.O. Box 10.011, Londrina 86057-970, Paran\u00e1, Brazil"},{"name":"Soil Biotechnology Laboratory, Embrapa Soja, P.O. Box 4006, Londrina 86085-981, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2053-3712","authenticated-orcid":false,"given":"Artur Berbel Lirio","family":"Rondina","sequence":"additional","affiliation":[{"name":"CNPq, Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, SHIS QI 1 Conjunto B, Lago Sul, Bras\u00edlia 71605-001, Federal District, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1446-328X","authenticated-orcid":false,"given":"Marcelo Mattos","family":"Cavallari","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste, Rod. Washington Luiz, Km 234, S\u00e3o Carlos 13560-970, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra Pereira","family":"Favero","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste, Rod. Washington Luiz, Km 234, S\u00e3o Carlos 13560-970, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7747-9839","authenticated-orcid":false,"given":"Marco Antonio","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Soil Biotechnology Laboratory, Embrapa Soja, P.O. Box 4006, Londrina 86085-981, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5132-8685","authenticated-orcid":false,"given":"Mariangela","family":"Hungria","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina, PR-445, km 380, P.O. Box 10.011, Londrina 86057-970, Paran\u00e1, Brazil"},{"name":"Soil Biotechnology Laboratory, Embrapa Soja, P.O. Box 4006, Londrina 86085-981, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"ref_1","unstructured":"USDA (United States Department of Agriculture) (2026, July 13). Foreign Agricultural Service. Livestock and Poultry: World Markets and Trade, Available online: https:\/\/apps.fas.usda.gov\/psdonline\/circulars\/livestock_poultry.pdf."},{"key":"ref_2","unstructured":"ABIEC (Associa\u00e7\u00e3o Brasileira das Ind\u00fastrias Exportadoras de Carnes) (2026, July 13). Beef Report: Perfil da Pecu\u00e1ria no Brasil. Available online: https:\/\/abiec.com.br\/publicacoes\/beef-report-2023\/."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Bolfe, E.L., De Castro Victoria, D., Sano, E.E., Bayma, G., Massruh\u00e1, S.M.F.S., and De Oliveira, A.F. (2024). Potential for agricultural expansion in degraded pasture lands in Brazil based on geospatial databases. Land, 13.","DOI":"10.3390\/land13020200"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Guimar\u00e3es, G.S., Rondina, A.B.L., Santos, M.S., Nogueira, M.A., and Hungria, M. (2022). Pointing out opportunities to increase grassland pastures productivity via microbial inoculants. Agronomy, 12.","DOI":"10.3390\/agronomy12081748"},{"key":"ref_5","first-page":"794","article-title":"Plant growth-promoting rhizobacteria impacting forage mass of Urochloa ruziziensis and Megathyrsus maximus","volume":"81","author":"Rondina","year":"2026","journal-title":"Grass Forage Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"109871","DOI":"10.1016\/j.agee.2025.109871","article-title":"Grassland soil carbon dynamics in southern Brazil","volume":"394","author":"Daboit","year":"2025","journal-title":"Agric. Ecosyst. Environ."},{"key":"ref_7","unstructured":"Dias-Filho, M.B. Diagn\u00f3stico das Pastagens no Brasil (Documentos 402), Embrapa Amaz\u00f4nia Oriental. Available online: http:\/\/www.infoteca.cnptia.embrapa.br\/infoteca\/handle\/doc\/986147."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.agee.2016.01.024","article-title":"Inoculation of Brachiaria spp. with the plant growth-promoting bacterium Azospirillum brasilense","volume":"221","author":"Hungria","year":"2016","journal-title":"Agric. Ecosyst. Environ."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Lopes, M.J.D.S., Dias-Filho, M.B., and Gurgel, E.S.C. (2021). Successful plant growth-promoting microbes: Inoculation methods and abiotic factors. Front. Sustain. Food Syst., 5.","DOI":"10.3389\/fsufs.2021.606454"},{"key":"ref_10","unstructured":"Macedo, M.C.M., Zimmer, A.H., Kichel, A.N., Almeida, R.G.d., and Araujo, A.R.d. (2013). Degrada\u00e7\u00e3o de pastagens, alternativas de recupera\u00e7\u00e3o e renova\u00e7\u00e3o, e formas de mitiga\u00e7\u00e3o. Anais do 1\u00ba Encontro de Aduba\u00e7\u00e3o de Pastagens da Scot Consultoria, Scot Consultoria."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Souza, M.N. (2021). Microrganismos simbiontes: Fixa\u00e7\u00e3o biol\u00f3gica de nitrog\u00eanio e recupera\u00e7\u00e3o de pastagens degradadas. T\u00f3picos em Recupera\u00e7\u00e3o de \u00c1reas Degradadas, M\u00e9rida Publishers.","DOI":"10.4322\/mp.978-65-991393-6-9"},{"key":"ref_12","unstructured":"Newton, W.E., and Nyman, C.T. (1976). Associative symbioses in tropical grasses. Proceedings of the International Symposium on Nitrogen Fixation, Pullman, WA, USA, 1974, Washington State University Press."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/S0038-0717(96)00226-X","article-title":"Biological nitrogen fixation in the tropics: Social and economic contributions","volume":"29","year":"1997","journal-title":"Soil Biol. Biochem."},{"key":"ref_14","unstructured":"University of Michigan, Center for Sustainable Systems (2026, July 13). Greenhouse Gases Factsheet (Pub. No. CSS05-21). Available online: https:\/\/css.umich.edu\/publications\/factsheets\/climate-change\/greenhouse-gases-factsheet."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e0200128","DOI":"10.36783\/18069657rbcs20200128","article-title":"Outstanding impact of Azospirillum brasilense strains Ab-V5 and Ab-V6 on the Brazilian agriculture","volume":"45","author":"Santos","year":"2021","journal-title":"Rev. Bras. Ci\u00eanc. Solo"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.soilbio.2016.08.020","article-title":"Azospirillum sp. in current agriculture: From the laboratory to the field","volume":"103","year":"2016","journal-title":"Soil Biol. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"22843","DOI":"10.1007\/s11356-022-18745-7","article-title":"Role of ACC deaminase producing bacteria for abiotic stress management and sustainable agriculture production","volume":"29","author":"Chandwani","year":"2022","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Vasques, N.C., Cerezini, P., Torres, A.R., Nogueira, M.A., and Hungria, M. (2026). Strategies to facilitate the bioprospection of properties of agronomic interest in microbial culture collections. BMC Microbiol., 26.","DOI":"10.1186\/s12866-025-04376-8"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/s00374-020-01453-0","article-title":"Changes in root morphological traits in soybean co-inoculated with Bradyrhizobium spp. and Azospirillum brasilense","volume":"56","author":"Rondina","year":"2020","journal-title":"Biol. Fertil. Soils"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s11104-021-04908-x","article-title":"Seed and leaf-spray inoculation of PGPR in brachiarias (Urochloa spp.)","volume":"463","author":"Hungria","year":"2021","journal-title":"Plant Soil"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2383","DOI":"10.32604\/phyton.2022.021507","article-title":"Participation of auxin transport in the early response of the Arabidopsis root system to inoculation with Azospirillum brasilense","volume":"91","year":"2022","journal-title":"Phyton"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"104276","DOI":"10.1016\/j.apsoil.2021.104276","article-title":"Meta-analysis of maize responses to Azospirillum brasilense inoculation in Brazil","volume":"170","author":"Barbosa","year":"2021","journal-title":"Appl. Soil Ecol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"843","DOI":"10.31413\/nat.v12i4.17576","article-title":"Solubiliza\u00e7\u00e3o de fosfatos e pot\u00e1ssio por bact\u00e9rias rizosf\u00e9ricas","volume":"12","author":"Majolo","year":"2024","journal-title":"Nativa"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Vasques, N.C., Nogueira, M.A., and Hungria, M. (2024). Increasing application of multifunctional Bacillus for biocontrol of pests and diseases and plant growth promotion. Agronomy, 14.","DOI":"10.3390\/agronomy14081654"},{"key":"ref_25","first-page":"1","article-title":"Revisi\u00f3n de las especies de Paspalum para Am\u00e9rica del Sur austral","volume":"102","author":"Zuloaga","year":"2005","journal-title":"Monogr. Syst. Bot. Mo. Bot. Gard."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Cidade, F.W., Vigna, B.B., De Souza, F.H., Valls, J.F.M., Dall\u2019Agnol, M., Zucchi, M.I., Souza-Chies, T.T., and Souza, A.P. (2013). Genetic variation in polyploid forage grass: Assessing the molecular genetic variability in the Paspalum genus. BMC Genet., 14.","DOI":"10.1186\/1471-2156-14-50"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1007\/s10681-017-1983-4","article-title":"Hybridization and heterosis in the Plicatula group of Paspalum","volume":"213","author":"Novo","year":"2017","journal-title":"Euphytica"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Acu\u00f1a, C.A., Mart\u00ednez, E.J., Zilli, A.L., Brugnoli, E.A., Espinoza, F., Marc\u00f3n, F., Urbani, M.H., and Quarin, C.L. (2019). Reproductive systems in Paspalum. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.01377"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s11104-015-2778-9","article-title":"Assessment of affinity and specificity of Azospirillum for plants","volume":"399","author":"Pereg","year":"2015","journal-title":"Plant Soil"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Guimar\u00e3es, G.S., Rondina, A.B.L., De Oliveira, A.G., Jank, L., Nogueira, M.A., and Hungria, M. (2023). Inoculation with plant growth-promoting bacteria improves the sustainability of tropical pastures with Megathyrsus maximus. Agronomy, 13.","DOI":"10.3390\/agronomy13030734"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e20200240","DOI":"10.1590\/1678-992x-2020-0240","article-title":"The occurrence of rhizobacteria from Paspalum genotypes and their effects on plant growth","volume":"79","author":"Amaral","year":"2021","journal-title":"Sci. Agric."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1146\/annurev.arplant.57.032905.105159","article-title":"The role of root exudates in rhizosphere interactions with plants and other organisms","volume":"57","author":"Bais","year":"2006","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e01026-20","DOI":"10.1128\/AEM.01026-20","article-title":"Specific root exudate compounds sensed by dedicated chemoreceptors shape Azospirillum brasilense chemotaxis in the rhizosphere","volume":"86","author":"Vo","year":"2020","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1111\/pce.14213","article-title":"Future roots for future soils","volume":"45","author":"Lynch","year":"2021","journal-title":"Plant Cell Environ."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Galindo-Casta\u00f1eda, T., Lynch, J.P., Six, J., and Hartmann, M. (2022). Improving soil resource uptake by plants through capitalizing on synergies between root architecture and root-associated microorganisms. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.827369"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1007\/s44279-025-00437-8","article-title":"Harnessing microbial communities to enhance seed germination","volume":"3","author":"Adeboye","year":"2025","journal-title":"Discov. Agric."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"127602","DOI":"10.1016\/j.micres.2024.127602","article-title":"Bacterial indole-3-acetic acid: A key regulator for plant growth, plant-microbe interactions, and agricultural adaptive resilience","volume":"281","author":"Etesami","year":"2024","journal-title":"Microbiol. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"100341","DOI":"10.1016\/j.stress.2023.100341","article-title":"Impact of plant growth-promoting rhizobacteria (PGPR) on plant nutrition and root characteristics","volume":"11","author":"Khoso","year":"2023","journal-title":"Plant Stress"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.micres.2013.09.009","article-title":"Bacteria with ACC deaminase can promote plant growth and help to feed the world","volume":"169","author":"Glick","year":"2013","journal-title":"Microbiol. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"107144","DOI":"10.1016\/j.isci.2023.107144","article-title":"Azospirillum brasilense activates peroxidase-mediated cell wall modification to inhibit root cell elongation","volume":"26","author":"Zhao","year":"2023","journal-title":"iScience"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1111\/nph.15738","article-title":"Root phenotypes for improved nutrient capture: An underexploited opportunity for global agriculture","volume":"223","author":"Lynch","year":"2019","journal-title":"New Phytol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1186\/s13568-017-0453-7","article-title":"Phytohormones and induction of plant-stress tolerance and defense genes by seed and foliar inoculation with Azospirillum brasilense cells and metabolites promote maize growth","volume":"7","author":"Fukami","year":"2017","journal-title":"AMB Express"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s13568-018-0608-1","article-title":"Azospirillum: Benefits that go far beyond biological nitrogen fixation","volume":"8","author":"Fukami","year":"2018","journal-title":"AMB Express"},{"key":"ref_44","unstructured":"Howieson, J.G., and Dilworth, M.J. (2016). Authentication of rhizobia and assessment of the legume symbiosis in controlled plant growth systems, Working with Rhizobia."},{"key":"ref_45","unstructured":"Hoagland, D.R., and Arnon, D.I. (1950). The Water-Culture Method for Growing Plants Without Soil, California Agricultural Experiment Station. Circular 347."},{"key":"ref_46","unstructured":"Howieson, J.G., and Dilworth, M.J. (2016). Isolation and growth of rhizobia, Working with Rhizobia, Chapter 3."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"995","DOI":"10.2307\/2258617","article-title":"A test of a modified line intersect method of estimating root length","volume":"63","author":"Tennant","year":"1975","journal-title":"J. Ecol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1017\/S0266467405002555","article-title":"Relationships among arbuscular mycorrhizas, root morphology and seedling growth of tropical native woody species in southern Brazil","volume":"21","author":"Zangaro","year":"2005","journal-title":"J. Trop. Ecol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/1467-842X.00109","article-title":"Theory & methods: A weighted least squares approach to Levene\u2019s test of homogeneity of variance","volume":"42","author":"Mathews","year":"2000","journal-title":"Aust. N. Z. J. Stat."},{"key":"ref_50","unstructured":"Brasil. Minist\u00e9rio da Agricultura, Pecu\u00e1ria e Abastecimento (Di\u00e1rio Oficial da Uni\u00e3o, 2011). Instru\u00e7\u00e3o Normativa n\u00ba 13, de 24 de mar\u00e7o de 2011. Aprova as normas sobre especifica\u00e7\u00f5es, garantias, registro, embalagem e rotulagem dos inoculantes destinados \u00e0 agricultura, Di\u00e1rio Oficial da Uni\u00e3o, se\u00e7\u00e3o 1."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1590\/1984-70332017v17n2s29","article-title":"Rbio: A tool for biometric and statistical analysis using the R platform","volume":"17","author":"Bhering","year":"2017","journal-title":"Crop Breed. Appl. Biotechnol."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2465\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T04:14:40Z","timestamp":1786853680000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2465"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,14]]},"references-count":51,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["plants15162465"],"URL":"https:\/\/doi.org\/10.3390\/plants15162465","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,14]]}}}