{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:31:03Z","timestamp":1787027463142,"version":"build-2736575974"},"reference-count":34,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32070218"],"award-info":[{"award-number":["32070218"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32400172"],"award-info":[{"award-number":["32400172"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32070218"],"award-info":[{"award-number":["32070218"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["32400172"],"award-info":[{"award-number":["32400172"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Scientific Research Startup Fund of Zhejiang A&F University","award":["2022LFR011"],"award-info":[{"award-number":["2022LFR011"]}]},{"name":"Scientific Research Startup Fund of Zhejiang A&F University","award":["2025LFR001"],"award-info":[{"award-number":["2025LFR001"]}]},{"award":["2022LFR011"],"award-info":[{"award-number":["2022LFR011"]}],"id":[{"id":"https:\/\/ror.org\/02vj4rn06","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2025LFR001"],"award-info":[{"award-number":["2025LFR001"]}],"id":[{"id":"https:\/\/ror.org\/02vj4rn06","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Guangxi Natural Science Foundation","award":["2022GXNSFBA035525"],"award-info":[{"award-number":["2022GXNSFBA035525"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Two new species of Carex (Cyperaceae), C. tianlinensis and C. leigongshanica, are described and illustrated based on morphological and molecular evidence. Phylogenetic analyses based on two nuclear (ETS and ITS) and three plastid (matK, trnL-F, and rpl32-trnL(UAG)) DNA markers recovered C. tianlinensis and C. leigongshanica as two distinct, strongly supported monophyletic lineages within the Indica clade. The two new species form a well-supported sister pair that is in turn sister to C. perakensis. Morphologically, C. tianlinensis most closely resembles C. perakensis, but differs in having inflorescences arising near the apex of the culms, pistillate part of spikes sparsely 2\u20136-flowered, and style glabrous. C. leigongshanica is also morphologically similar to C. perakensis, but differs in having each inflorescence with 6\u201316 shorter spikes, spikes 0.7\u20131.5 cm long, sessile or subsessile, utricles shorter, 3\u20133.5 mm long, and leaf blades narrower, 3\u20135.5 mm wide. Detailed morphological descriptions, type designations, specimen citations, and information on habitat and phenology are provided for the two new species.<\/jats:p>","DOI":"10.3390\/plants15162481","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T08:05:41Z","timestamp":1786953941000},"page":"2481","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Carex\u00a0tianlinensis and C. leigongshanica, Two New Species Related to C. perakensis from Southwest China, Based on Morphological and Molecular Evidence"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1725-0247","authenticated-orcid":false,"given":"Zhaocen","family":"Lu","sequence":"first","affiliation":[{"name":"School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China"},{"name":"Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenguo","family":"Xie","sequence":"additional","affiliation":[{"name":"Administration of Guizhou Leigongshan National Nature Reserve, Leishan 557199, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2683-206X","authenticated-orcid":false,"given":"Ying","family":"Qin","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Jin","sequence":"additional","affiliation":[{"name":"School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1111\/jse.12757","article-title":"A new classification of Cyperaceae (Poales) supported by phylogenomic data","volume":"59","author":"Larridon","year":"2021","journal-title":"J. Syst. Evol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1600\/036364416X692488","article-title":"Clarification of the use of the terms perigynium and utricle in Carex L. (Cyperaceae)","volume":"41","author":"Wilson","year":"2016","journal-title":"Syst. Bot."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Global Carex Group (2015). Making Carex Monophyletic (Cyperaceae, tribe Cariceae): A new broader circumscription. Bot. J. Linn. Soc., 179, 1\u201342.","DOI":"10.1111\/boj.12298"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1111\/jse.12549","article-title":"A tale of worldwide success: Behind the scenes of Carex (Cyperaceae) biogeography and diversification","volume":"57","author":"Villaverde","year":"2019","journal-title":"J. Syst. Evol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1111\/jse.12722","article-title":"A framework infrageneric classification of Carex (Cyperaceae) and its organizing principles","volume":"59","author":"Roalson","year":"2021","journal-title":"J. Syst. Evol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"24106","DOI":"10.17520\/biods.2024106","article-title":"An updated species checklist and taxonomic synopsis of Cyperaceae in China","volume":"32","author":"Li","year":"2024","journal-title":"Biodivers. Sci."},{"key":"ref_7","first-page":"349","article-title":"Carex L","volume":"Volume 23","author":"Wu","year":"2010","journal-title":"Flora of China"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1038\/35002501","article-title":"Biodiversity hotspots for conservation priorities","volume":"403","author":"Myers","year":"2000","journal-title":"Nature"},{"key":"ref_9","first-page":"971","article-title":"New records of Carex L. (Cyperaceae) from Guangxi, China","volume":"45","author":"Lu","year":"2025","journal-title":"Guihaia"},{"key":"ref_10","first-page":"2203","article-title":"Notes on Carex L. (Cyperaceae) in Guangxi","volume":"43","author":"Lu","year":"2023","journal-title":"Guihaia"},{"key":"ref_11","first-page":"620","article-title":"Carex L","volume":"Volume 8","author":"Li","year":"1988","journal-title":"Flora of Guizhou"},{"key":"ref_12","unstructured":"Engler, H. (1909). Cyperaceae-Caricoideae. Das Pflanzenreich, W. Engelmann."},{"key":"ref_13","first-page":"1","article-title":"Carices Indochinenses necnon Siamenses","volume":"53","author":"Raymond","year":"1959","journal-title":"M\u00e9m. Jard. Bot. Montr."},{"key":"ref_14","first-page":"221","article-title":"Carex","volume":"Volume 12","author":"Dai","year":"2000","journal-title":"Flora Reipublicae Popularis Sinicae"},{"key":"ref_15","first-page":"197","article-title":"Carex sect. Decorae (K\u00fck.) Ohwi","volume":"Volume 12","author":"Jin","year":"2019","journal-title":"Flora of Pan-Himalaya"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1139\/b90-180","article-title":"Evolution in Sedges (Carex, Cyperaceae)","volume":"68","author":"Reznicek","year":"1990","journal-title":"Can. J. Bot."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1111\/jse.12731","article-title":"Geographical vs. ecological diversification in Carex section Phacocystis (Cyperaceae): Patterns hidden behind a twisted taxonomy","volume":"59","author":"Gebauer","year":"2021","journal-title":"J. Syst. Evol."},{"key":"ref_18","unstructured":"Jin, X.F., and Zheng, C.Z. (2013). Taxonomy of Carex section Rhomboidales (Cyperaceae), Science Press."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/BF02860862","article-title":"The Inflorescence of Carex and related genera","volume":"42","author":"Smith","year":"1976","journal-title":"Bot. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Edwards, K.R., Glocker, B., Mastn\u00fd, J., and Picek, T. (2025). Changing environmental conditions impact the phenotypic plasticity of Carex acuta and Glyceria maxima, two common wet grassland species. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1542907"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1007\/s00606-015-1237-7","article-title":"Molecular and morphological perspectives on the circumscription of Carex section Heleoglochin (Cyperaceae)","volume":"301","author":"Molina","year":"2015","journal-title":"Plant Syst. Evol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1093\/botlinnean\/boy040","article-title":"Reconciling morphology and phylogeny allows an integrative taxonomic revision of the giant Sedges of Carex section Rhynchocystis (Cyperaceae)","volume":"188","year":"2018","journal-title":"Bot. J. Linn. Soc."},{"key":"ref_23","first-page":"11","article-title":"A rapid DNA isolation procedure for small quantities of fresh leaf tissue","volume":"19","author":"Doyle","year":"1987","journal-title":"Phytochem. Bull."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1111\/jse.12795","article-title":"Revisiting Carex sect. Confertiflorae s.l. (Cyperaceae): New data from molecular and morphological evidence and first insights on Carex biogeography in East Asia","volume":"59","author":"Lu","year":"2021","journal-title":"J. Syst. Evol."},{"key":"ref_25","first-page":"71","article-title":"DNASTAR\u2019s Lasergene sequence analysis software","volume":"132","author":"Burland","year":"2000","journal-title":"Methods Mol. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1038\/nmeth.2109","article-title":"jModelTest 2: More models, new heuristics and parallel computing","volume":"9","author":"Darriba","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1111\/1755-0998.13096","article-title":"PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies","volume":"20","author":"Zhang","year":"2020","journal-title":"Mol. Ecol. Resour."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1038\/s41467-019-08822-w","article-title":"Model selection may not be a mandatory step for phylogeny reconstruction","volume":"10","author":"Abadi","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies","volume":"30","author":"Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_31","first-page":"539","article-title":"MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space","volume":"61","author":"Ronquist","year":"2012","journal-title":"Syst. Bot."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"W587","DOI":"10.1093\/nar\/gkad359","article-title":"tvBOT: A web application for visualizing, modifying and annotating phylogenetic trees","volume":"51","author":"Xie","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_33","unstructured":"IUCN Standards and Petitions Committee (2026, June 30). Guidelines for Using the IUCN Red List Categories and Criteria. Version 16. IUCN Species Survival Commission. Available online: https:\/\/www.iucnredlist.org\/resources\/redlistguidelines."},{"key":"ref_34","first-page":"84","article-title":"Analysis on Seed Plants in Guizhou Leigongshan National Nature Reserve","volume":"1","author":"Xie","year":"2010","journal-title":"For. Resour. Manag."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2481\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:23:29Z","timestamp":1787027009000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2481"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,16]]},"references-count":34,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["plants15162481"],"URL":"https:\/\/doi.org\/10.3390\/plants15162481","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,16]]}}}