{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T11:25:09Z","timestamp":1786965909700,"version":"build-2736575974"},"reference-count":57,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Youth Fund Project of the Basic Research Program of the Science and Technology Department of Qinghai Province, China","award":["2023-ZJ-963Q"],"award-info":[{"award-number":["2023-ZJ-963Q"]}]},{"award":["2023-ZJ-963Q"],"award-info":[{"award-number":["2023-ZJ-963Q"]}],"id":[{"id":"https:\/\/ror.org\/03vz7b375","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Syringa oblata is a renowned flowering shrub, yet the functional studies on the regulatory factors governing its flower color formation remain limited. In this study, a new MYB transcription factor, named SoMYB1, was isolated from the petals of Syringa oblata by the homologous cloning technique. The open reading frames of SoMYB1 was 705 bp in length. Amino acid sequence analysis showed that SoMYB1 contained the highly conserved R2 and R3 MYB domains. A quantitative real-time PCR analysis revealed that SoMYB1 was expressed tissue specifically in flowers. Ectopic expression of SoMYB1 induced anthocyanins accumulation in both vegetative and reproductive tissues of transgenic tobacco lines. Overexpression of SoMYB1 in tobacco enhanced the expression of NtAN2 (MYB) and NtAN1b (bHLH), and the expression of structural genes NtCHS, NtCHI, NtF3H, NtF3\u2032H, NtDFR, NtANS, NtUFGT, and Nt3RT increased remarkably. The UPLC-MS\/MS analysis of transgenic tobacco leaves showed that the heterologous expression of SoMYB1 significantly promoted the accumulation of anthocyanin metabolites, especially cyanidin, which accounted for more than 50% of the total anthocyanins in the transgenic tobacco lines. This study elucidates the molecular mechanism by which SoMYB1 positively regulates anthocyanin biosynthesis in S. oblata, providing theoretical and technical support for developing plant resources rich in cyanidin.<\/jats:p>","DOI":"10.3390\/plants15162492","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T10:04:36Z","timestamp":1786961076000},"page":"2492","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Heterologous Expression of SoMYB1 Derived from Syringa oblata Enhances Cyanidin Biosynthesis in Tobacco"],"prefix":"10.3390","volume":"15","author":[{"given":"Yinglong","family":"Yang","sequence":"first","affiliation":[{"name":"Qinghai University, Xining 810016, China"},{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Du","sequence":"additional","affiliation":[{"name":"Qinghai University, Xining 810016, China"},{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Yang","sequence":"additional","affiliation":[{"name":"Zhengzhou Institute of Urban Landscape Science, Zhengzhou 450051, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Wang","sequence":"additional","affiliation":[{"name":"Qinghai University, Xining 810016, China"},{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"},{"name":"Qinghai Plateau Key Laboratory of Tree Genetics and Breeding, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangji","family":"Ye","sequence":"additional","affiliation":[{"name":"Qinghai University, Xining 810016, China"},{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"},{"name":"Qinghai University Key Laboratory of Tibet Plateau Biotechnology of the Ministry of Education, Xining 810016, China"},{"name":"Laboratory for Research and Utilization of Tibet Plateau Germplasm Resources, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijun","family":"Li","sequence":"additional","affiliation":[{"name":"Qinghai University, Xining 810016, China"},{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"},{"name":"Qinghai Plateau Key Laboratory of Tree Genetics and Breeding, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Zheng","sequence":"additional","affiliation":[{"name":"Qinghai University, Xining 810016, China"},{"name":"Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China"},{"name":"Qinghai Plateau Key Laboratory of Tree Genetics and Breeding, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1017\/S0007114514001482","article-title":"Anthocyanins from fruit juices improve the antioxidant status of healthy young female volunteers without affecting anti-inflammatory parameters: Results from the randomised, double-blind, placebo-controlled, cross-over ANTHONIA (ANTHOcyanins in Nutrition Investigation Alliance) study","volume":"112","author":"Kuntz","year":"2014","journal-title":"Br. 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