{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T05:23:45Z","timestamp":1787289825890,"version":"build-2736575974"},"reference-count":13,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003339","name":"Spanish National Research Council","doi-asserted-by":"crossref","award":["GO AEI MR33 1B"],"award-info":[{"award-number":["GO AEI MR33 1B"]}],"id":[{"id":"10.13039\/501100003339","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003339","name":"Spanish National Research Council","doi-asserted-by":"crossref","award":["FEADER 2023(045B)"],"award-info":[{"award-number":["FEADER 2023(045B)"]}],"id":[{"id":"10.13039\/501100003339","id-type":"DOI","asserted-by":"crossref"}]},{"award":["GO AEI MR33 1B"],"award-info":[{"award-number":["GO AEI MR33 1B"]}],"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}]},{"award":["FEADER 2023(045B)"],"award-info":[{"award-number":["FEADER 2023(045B)"]}],"id":[{"id":"https:\/\/ror.org\/02gfc7t72","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Olive trees (Olea europaea L.) show propagation behaviour that is strongly dependent on genotype. A dozen previously unknown autochthonous olive varieties from Galicia (north-western Spain) have recently been identified through molecular and botanical characterisation techniques. However, their suitability for vegetative propagation remains unknown. Survival, callus formation and rooting were evaluated in semi-hardwood cuttings of 12 autochthonous Galician varieties and the control variety Cobran\u00e7osa in two trials carried out in winter\u2013spring and summer-\u2013autumn 2025 (676 and 622 cuttings, respectively). Binary variables were analysed using generalised linear models (binomial distribution, logit link). The variety effect was highly significant for all three variables (p &lt; 0.0001), while the cutting date was not significant as a main effect; the variety \u00d7 date interaction was significant for survival and rooting but not for callus formation. Mean rooting increased from 19% in winter to 61% in summer, although some varieties showed the opposite pattern. A strong negative correlation was found between flowering on the mother plant and rooting, and no correlation was found between callus formation and rooting. These results provide a first quantitative reference on the vegetative propagation of autochthonous Galician olive varieties and supply baseline information for their multiplication and conservation.<\/jats:p>","DOI":"10.3390\/plants15162506","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:14:01Z","timestamp":1787145241000},"page":"2506","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Semi-Hardwood Cutting Propagation of Autochthonous Olive Varieties from Galicia: A First Evaluation of Rooting Ability"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7822-0538","authenticated-orcid":false,"given":"Adri\u00e1n","family":"L\u00f3pez-Villamor","sequence":"first","affiliation":[{"name":"Grupo VIOR, Misi\u00f3n Biol\u00f3gica de Galicia (Consejo Superior de Investigaciones Cient\u00edficas\u2014CSIC), Carballeira 8, Salcedo, 36143 Pontevedra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3736-3991","authenticated-orcid":false,"given":"Susana","family":"Boso","sequence":"additional","affiliation":[{"name":"Grupo VIOR, Misi\u00f3n Biol\u00f3gica de Galicia (Consejo Superior de Investigaciones Cient\u00edficas\u2014CSIC), Carballeira 8, Salcedo, 36143 Pontevedra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6170-9879","authenticated-orcid":false,"given":"Pilar","family":"Gago","sequence":"additional","affiliation":[{"name":"Grupo VIOR, Misi\u00f3n Biol\u00f3gica de Galicia (Consejo Superior de Investigaciones Cient\u00edficas\u2014CSIC), Carballeira 8, Salcedo, 36143 Pontevedra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0656-5665","authenticated-orcid":false,"given":"Jos\u00e9-Luis","family":"Santiago","sequence":"additional","affiliation":[{"name":"Grupo VIOR, Misi\u00f3n Biol\u00f3gica de Galicia (Consejo Superior de Investigaciones Cient\u00edficas\u2014CSIC), Carballeira 8, Salcedo, 36143 Pontevedra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0653-8015","authenticated-orcid":false,"given":"Mar\u00eda-Carmen","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Grupo VIOR, Misi\u00f3n Biol\u00f3gica de Galicia (Consejo Superior de Investigaciones Cient\u00edficas\u2014CSIC), Carballeira 8, Salcedo, 36143 Pontevedra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e0702","DOI":"10.5424\/sjar\/2019172-13572","article-title":"The Forgotten, Ancient Olive Trees of the Spanish Northwest: A First Molecular and Botanical Analysis","volume":"17","author":"Gago","year":"2019","journal-title":"Span. J. Agric. Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Gago, P., Boso, S., Santiago, J.L., and Mart\u00ednez, M.C. (2024). Identification and Characterization of Relict Olive Varieties (Olea europaea L.) in the Northwest of the Iberian Peninsula. Horticulturae, 10.","DOI":"10.3390\/horticulturae10020175"},{"key":"ref_3","unstructured":"Hartmann, H.T., Kester, D.E., Davies, F.T., and Geneve, R.L. (2011). Hartmann & Kester\u2019s Plant Propagation: Principles and Practices, Prentice Hall. [8th ed.]."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Velada, I., Grzebelus, D., Lousa, D., M. Soares, C., Santos Macedo, E., Peixe, A., Arnholdt-Schmitt, B., and G. Cardoso, H. (2018). AOX1-Subfamily Gene Members in Olea europaea Cv. \u201cGalega Vulgar\u201d\u2014Gene Characterization and Expression of Transcripts during IBA-Induced in Vitro Adventitious Rooting. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19020597"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.scienta.2003.08.008","article-title":"Growing Season and Hydrogen Peroxide Effects on Root Induction and Development in Olea europaea L. (Cvs \u2018Frantoio\u2019 and \u2018Gentile Di Larino\u2019) Cuttings","volume":"100","author":"Sebastiani","year":"2004","journal-title":"Sci. Hortic."},{"key":"ref_6","first-page":"58","article-title":"Effect of the Application of IBA and Date of Collection on the Rooting of Semi-Hardwood Cuttings of Olive (Olea europaea L.) Cultivar \u2018Sevillano\u2019","volume":"74","author":"Ruiz","year":"1998","journal-title":"Olivae"},{"key":"ref_7","unstructured":"Alonso de Herrera, G. (1513). Agricultura General, Imprenta Real."},{"key":"ref_8","unstructured":"G\u00f3mez, L., Fraga, M., and Vigo, C. (2025). Producci\u00f3n mediante cultivo in vitro de variedades aut\u00f3ctonas de olivo gallego. Libro de Res\u00famenes del SECIVTV 2025, SECIVTV."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1139\/cjfr-2016-0397","article-title":"Adventitious Root Formation in Castanea Sp. Semi-Hard Cuttings Is under Moderate Genetic Control Caused Mainly by Non-Additive Genetic Variance","volume":"47","year":"2017","journal-title":"Can. J. For. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.scienta.2015.11.034","article-title":"Reviewing current knowledge on olive (Olea europaea L.) adventitious root formation","volume":"198","author":"Cabrita","year":"2016","journal-title":"Sci. Hortic."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Vahdati, K., Sadeghi-Majd, R., Sestras, A.F., Licea-Moreno, R.J., Peixe, A., and Sestras, R.E. (2022). Clonal Propagation of Walnuts (Juglans spp.): A Review on Evolution from Traditional Techniques to Application of Biotechnology. Plants, 11.","DOI":"10.3390\/plants11223040"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1007\/s00122-026-05197-7","article-title":"Dissecting the genetic architecture of early root-related traits in grapevine rootstocks across propagation methods and different scion","volume":"139","author":"Morel","year":"2026","journal-title":"Theor. Appl. Genet."},{"key":"ref_13","unstructured":"SAS Institute Inc. (2015). SAS\/STAT 14.1 User\u2019s Guide, SAS Institute Inc."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2506\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T04:28:15Z","timestamp":1787286495000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":13,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["plants15162506"],"URL":"https:\/\/doi.org\/10.3390\/plants15162506","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]}}}