{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T09:25:47Z","timestamp":1787217947521,"version":"build-2736575974"},"reference-count":51,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"General Program of Science and Technology & Education Joint Fund of Sichuan Province","award":["2026NSFSC2016"],"award-info":[{"award-number":["2026NSFSC2016"]}]},{"DOI":"10.13039\/501100013792","name":"Chengdu University","doi-asserted-by":"publisher","award":["Z3785"],"award-info":[{"award-number":["Z3785"]}],"id":[{"id":"10.13039\/501100013792","id-type":"DOI","asserted-by":"publisher"}]},{"award":["Z3785"],"award-info":[{"award-number":["Z3785"]}],"id":[{"id":"https:\/\/ror.org\/034z67559","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Herpetospermum pedunculosum is an endangered cucurbitaceous medicinal plant whose functional genomic and metabolic studies are constrained by the lack of efficient aseptic seedling and genetic transformation systems. Here, we established a rapid aseptic seedling production method and a RUBY-assisted hairy root transformation platform integrated with metabolomic evaluation. The cotton-supported liquid culture method provided a favorable balance between germination performance and experimental operability. Microsyringe-mediated stem-base inoculation with Rhizobium rhizogenes K599 carrying 35S::RUBY enabled rapid visual screening of RUBY-positive hairy roots, with a maximum transformation efficiency of 93.33%. Widely targeted metabolomics identified 242 metabolites and revealed major differences in amino acid\/nitrogen and sugar metabolism, lipid-related metabolism, specialized metabolite biosynthesis, hormone-related pathways, and ABC transporters among wild-type, vector-free K599-induced, and RUBY-expressing roots. In addition, 80 HpRHL-like bHLH genes were identified. As a proof of concept, RT-qPCR confirmed HpRHL76 overexpression, which was associated with an increase in mean root hair length from approximately 0.4 to 1.1 mm. Together, this integrated framework provides a practical basis for preliminary candidate gene assessment, functional genomic studies, and future metabolic engineering of medicinally relevant compounds in H. pedunculosum, and may serve as a methodological reference for other difficult-to-transform medicinal plants.<\/jats:p>","DOI":"10.3390\/plants15162514","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T08:10:20Z","timestamp":1787213420000},"page":"2514","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A RUBY-Assisted Hairy Root Transformation Platform for Metabolomic Profiling and Preliminary Functional Analysis of HpRHL76 in Herpetospermum pedunculosum"],"prefix":"10.3390","volume":"15","author":[{"given":"Shiyu","family":"Yao","sequence":"first","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"},{"name":"Sichuan-Xizang Medicinal Resource Breeding and Standardization Team, Institute for Advanced Study, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1753-9491","authenticated-orcid":false,"given":"Yujie","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, National Agricultural Science & Technology Center, Chengdu 610213, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyue","family":"Wang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"},{"name":"Sichuan-Xizang Medicinal Resource Breeding and Standardization Team, Institute for Advanced Study, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinghui","family":"Wu","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"},{"name":"Sichuan-Xizang Medicinal Resource Breeding and Standardization Team, Institute for Advanced Study, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingrong","family":"Liu","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5623-5974","authenticated-orcid":false,"given":"Min","family":"Sun","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"},{"name":"Sichuan-Xizang Medicinal Resource Breeding and Standardization Team, Institute for Advanced Study, Chengdu University, Chengdu 610106, China"},{"name":"Natural Products Chem-Bio Innovation Center, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziwei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"},{"name":"Sichuan-Xizang Medicinal Resource Breeding and Standardization Team, Institute for Advanced Study, Chengdu University, Chengdu 610106, China"},{"name":"Natural Products Chem-Bio Innovation Center, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1926-6496","authenticated-orcid":false,"given":"Bingliang","family":"Liu","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuxia","family":"Lu","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"},{"name":"Sichuan-Xizang Medicinal Resource Breeding and Standardization Team, Institute for Advanced Study, Chengdu University, Chengdu 610106, China"},{"name":"Natural Products Chem-Bio Innovation Center, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixi","family":"Yang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"},{"name":"Sichuan-Xizang Medicinal Resource Breeding and Standardization Team, Institute for Advanced Study, Chengdu University, Chengdu 610106, China"},{"name":"Natural Products Chem-Bio Innovation Center, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2168-6294","authenticated-orcid":false,"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"},{"name":"Sichuan-Xizang Medicinal Resource Breeding and Standardization Team, Institute for Advanced Study, Chengdu University, Chengdu 610106, China"},{"name":"Natural Products Chem-Bio Innovation Center, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0257-6367","authenticated-orcid":false,"given":"Qi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"},{"name":"Sichuan-Xizang Medicinal Resource Breeding and Standardization Team, Institute for Advanced Study, Chengdu University, Chengdu 610106, China"},{"name":"Natural Products Chem-Bio Innovation Center, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0961-9542","authenticated-orcid":false,"given":"Yang","family":"Tao","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Sichuan-Xizang Traditional Medicinal Plant, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China"},{"name":"Sichuan-Xizang Medicinal Resource Breeding and Standardization Team, Institute for Advanced Study, Chengdu University, Chengdu 610106, China"},{"name":"Natural Products Chem-Bio Innovation Center, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1002\/bmc.1028","article-title":"Simultaneous determination of seven bioactive lignans in Herpetospermum caudigerum by RP-HPLC 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