{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T04:22:23Z","timestamp":1787458943328,"version":"build-2736575974"},"reference-count":42,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Open Fund of State Key Laboratory of Aridland Crop Science of China","award":["GSCS-2024-11"],"award-info":[{"award-number":["GSCS-2024-11"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32560474"],"award-info":[{"award-number":["32560474"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32560474"],"award-info":[{"award-number":["32560474"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Major Science and Technology Project of Gansu Province","award":["24ZDNA007"],"award-info":[{"award-number":["24ZDNA007"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["CARS-12"],"award-info":[{"award-number":["CARS-12"]}]},{"award":["CARS-12"],"award-info":[{"award-number":["CARS-12"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Winter rapeseed suffers severe damage under cold stress, which seriously restricts its growth and yield. However, differences among cultivars in leaf starch and soluble sugar, as well as their underlying molecular regulatory mechanisms under low-temperature stress, remain poorly understood. We hypothesized that winter rapeseed cultivars with differences in cold tolerance would vary in starch accumulation, soluble sugar contents, and expression of the starch and sugar metabolism-related genes under cold stress. This study aimed to investigate differences in starch accumulation, soluble sugar content, and the gene expression patterns involved in the starch and sugar metabolism pathway in the leaves of winter rapeseed (Brassica napus L.) cultivars with contrasting cold tolerance under cold stress. In this study, two winter rapeseed cultivars, cold-tolerant L88 and cold-sensitive T2288, received 24 h cold treatments at 22 \u00b0C (control), 4 \u00b0C, 0 \u00b0C, and \u22124 \u00b0C. Plant morphology and leaf ultrastructure were observed, iodine\u2013potassium iodide (I2-KI) staining was performed, and the starch, soluble sugar contents (sucrose, fructose, glucose, and maltose) were analyzed for each temperature regime. Transcriptome sequencing was performed exclusively at \u22124 \u00b0C (0 h versus 24 h). Under \u22124 \u00b0C stress, the two cultivars showed the greatest differences in plant phenotype and leaf ultrastructure, with the cold-sensitive cultivar Tianyou 2288 suffering more severe freezing injury. In Longyou 88, chloroplasts in mesophyll cells were swollen and contained fewer starch granules, whereas in Tianyou 2288 some chloroplasts disintegrated and large numbers of starch granules remained undegraded. As the treatment temperature decreased, leaf starch content declined in both cultivars. At \u22124 \u00b0C, starch content in Longyou 88 was 3.84 mg\/g lower than in Tianyou 2288. The contents of soluble sugars, sucrose, glucose, and maltose in leaves increased continuously in both cultivars as stress temperature decreased and reached their maximum values at \u22124 \u00b0C. However, fructose content showed cultivar-specific changes. In Longyou 88, fructose content reached its maximum value of 12.59 mg\/g at \u22124 \u00b0C, whereas in Tianyou 2288 it peaked at 7.54 mg\/g at 4 \u00b0C. At \u22124 \u00b0C, fructose content was 6.69 mg\/g higher in Longyou 88 than in Tianyou 2288. Transcriptome analysis revealed that after 24 h of \u22124 \u00b0C stress, Longyou 88 had more upregulated differentially expressed genes (DEGs) than Tianyou 2288, whereas the number of downregulated DEGs in Tianyou 2288 was approximately twice that in Longyou 88. The genes BAM1, BAM3, SUS1, SCRK1, TPS9, and E1314, which were involved in the starch and sucrose metabolism pathway, were upregulated in both cultivars, whereas BGL44 was downregulated. These findings indicate that cold-tolerance winter rapeseed is associated with enhanced starch degradation, maintenance of chloroplast structural integrity in mesophyll cells, and increased soluble sugar accumulation, providing potential physiological indicators for cold-tolerance evaluation.<\/jats:p>","DOI":"10.3390\/plants15162534","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T09:31:59Z","timestamp":1787304719000},"page":"2534","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Starch Degradation and Soluble Sugar Accumulation Are Associated with Cold Tolerance in Winter Rapeseed (Brassica napus L.)"],"prefix":"10.3390","volume":"15","author":[{"given":"Lin","family":"Long","sequence":"first","affiliation":[{"name":"College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Pu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyan","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Seed Industry Research Institute of Gansu Provincial University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0753-7113","authenticated-orcid":false,"given":"Li","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Aridland Crop Science, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Aridland Crop Science, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wangtian","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wancang","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1270\/jsbbs.16161","article-title":"Analysis of cold resistance and identification of SSR markers linked to cold resistance genes in Brassica rapa L.","volume":"67","author":"Huang","year":"2017","journal-title":"Breed. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1046\/j.1365-3040.2000.00560.x","article-title":"The ultrastructure of chilling stress","volume":"23","author":"Kratsch","year":"2000","journal-title":"Plant Cell Environ."},{"key":"ref_3","first-page":"108","article-title":"Changes in ultrastructure of tomato cells under low temperature stress","volume":"41","author":"Zhang","year":"2012","journal-title":"Henan Agric. Sci."},{"key":"ref_4","first-page":"5","article-title":"Regulation of Plant Growth Regulator on Frost Resistance of Cold Season Turfgrass","volume":"7","author":"Guan","year":"2018","journal-title":"Bot. Res."},{"key":"ref_5","first-page":"106","article-title":"Comparison of leaf blade ultra-structures of two camphor varieties under cold stress","volume":"41","author":"Wang","year":"2013","journal-title":"J. Northwest AF Univ. (Nat. Sci. Ed.)"},{"key":"ref_6","first-page":"1925","article-title":"Effect of Low Temperature Stress on Leaf Submicroscopic Structureand Chlorophyll Fluorescence Parameters of Cassava \u2018F200\u2019 Seedlings","volume":"42","author":"Wang","year":"2021","journal-title":"Chin. J. Trop. Crops"},{"key":"ref_7","unstructured":"Zhang, H. (2007). The Effects of Cold Stress on Ultrastructure and Physiological Metabolism in Eriobotrya japonica Lindl. [Ph.D. Dissertation, Fujian Agriculture and Forestry University]."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Pu, Y., Liu, L., Wu, J., Zhao, Y., Bai, J., Ma, L., Yue, J., Jin, J., Niu, Z., and Fang, Y. (2019). Transcriptome Profile Analysis of Winter Rapeseed (Brassica napus L.) in Response to Freezing Stress, Reveal Potentially Connected Events to Freezing Stress. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20112771"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"F\u00fcrtauer, L., Weiszmann, J., Weckwerth, W., and N\u00e4gele, T. (2019). Dynamics of Plant Metabolism during Cold Acclimation. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20215411"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Lintunen, A., Paljakka, T., Jyske, T., Peltoniemi, M., Sterck, F., Von, A.G., Cochard, H., Copini, P., Caldeira, M.C., and Delzon, S. (2016). Osmolality and Non-Structural Carbohydrate Composition in the Secondary Phloem of Trees across a Latitudinal Gradient in Europe. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00726"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1093\/treephys\/tpr099","article-title":"Localized stem chilling alters carbon processes in the adjacent stem and in source leaves","volume":"31","author":"De","year":"2011","journal-title":"Tree Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1093\/treephys\/tpt021","article-title":"Effects of dormancy progression and low-temperature response on changes in the sorbitol concentration in xylem sap of Japanese pear during winter season","volume":"33","author":"Ito","year":"2013","journal-title":"Tree Physiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.1399-3054.2006.00604.x","article-title":"Roles of \u03b2-amylase and starch breakdown during temperatures stress","volume":"126","author":"Kaplan","year":"2006","journal-title":"Physiol. Plant."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1093\/treephys\/25.11.1389","article-title":"Low temperature responses of Nothofagus dombeyi and Nothofagus nitida, two evergreen species from south central Chile","volume":"25","author":"Alberdi","year":"2005","journal-title":"Tree Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1104\/pp.104.040808","article-title":"\u03b2-Amylase Induction and the Protective Role of Maltose during Temperature Shock","volume":"135","author":"Kaplan","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2742","DOI":"10.1016\/S2095-3119(18)62147-1","article-title":"Physiological and molecular responses to cold stress in rapeseed (Brassica napus L.)","volume":"18","author":"Lei","year":"2019","journal-title":"J. Integr. Agric."},{"key":"ref_17","unstructured":"Peng, T. (2013). Suppression Subtractive Hybridization cDNA Library Construction for Low Temperature-Treated Poncirus Trifoliata and Characterization of PtrBAMl. [Ph.D. Dissertation, Huazhong Agricultural University]."},{"key":"ref_18","first-page":"300","article-title":"Histochemical characteristics and differentiation of the belowground buds of Medicago archiducisnicolai during overwintering","volume":"39","author":"Yang","year":"2022","journal-title":"Pratacult. Sci."},{"key":"ref_19","unstructured":"Yue, C. (2015). Cloning and Expression Analysis of Sugar-related Genes during Cold Acclimation in Tea Plant. [Ph.D. Dissertation, Chinese Academy of Agricultural Sciences]."},{"key":"ref_20","first-page":"2716","article-title":"Study on Adaptation and Introduction Possibility of Winter Rapeseed to Dry and Cold Areas in Northwest China","volume":"40","author":"Sun","year":"2007","journal-title":"Sci. Agric. Sin."},{"key":"ref_21","unstructured":"Sun, B. (2022). Preliminary Study on the Changes of Fatty Acid Composition and the Function of FAD3 and SDR in Brassica rapa L. Under Low Temperature Stress. [Master\u2019s Thesis, Gansu Agricultural University]."},{"key":"ref_22","first-page":"116","article-title":"Variations of the Ultrastructure of the Leaf Cell of Cotton under Low Temperature","volume":"21","author":"Yang","year":"2001","journal-title":"J. Shanxi Agric. Univ."},{"key":"ref_23","first-page":"6","article-title":"Relationship Between Cold Tolerance and Leaf Structure of the Three Species Sedum","volume":"19","author":"Wang","year":"2013","journal-title":"Chin. J. Appl. Environ. Biol."},{"key":"ref_24","first-page":"170","article-title":"Analysis on Leaf Microstructure and Ultrastructure of Two Different Frost-resistant Wild Materials of Solanum tuberosum","volume":"44","author":"Wu","year":"2025","journal-title":"Seed"},{"key":"ref_25","first-page":"34","article-title":"Effects of low temperature on mesophyll cell ultrastructure of Osmanthus fragrans \u2018Wandian Jin\u2019","volume":"60","author":"You","year":"2021","journal-title":"J. Sun Yat-Sen Univ. (Nat. Sci. Ed.)"},{"key":"ref_26","unstructured":"Hu, H. (2025). Evaluation of Cold Tolerance and Identification of Critical Cold Tolerance Genes in 295 Core Germplasm Resources of Brassica napus L.. [Master\u2019s Thesis, Northwest A&F University]."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1111\/j.1365-3040.2007.01763.x","article-title":"Multilevel genomic analysis of the response of transcripts, enzyme activities and metabolites in Arabidopsis rosettes to a progressive decrease of temperature in the non-freezing range","volume":"31","author":"Usadel","year":"2008","journal-title":"Plant Cell Environ."},{"key":"ref_28","unstructured":"Liu, T. (2023). The Function and Molecular Mechanism of ABA-Mediated Starch and Sugar Metabolism in Potato Freezing Tolerance. [Ph.D. Dissertation, Huazhong Agricultural University]."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1271\/bbb.100813","article-title":"Detection of Sugar Accumulation and Expression Levels of Correlative Key Enzymes in Winter Wheat (Triticum aestivum) at Low Temperatures","volume":"75","author":"Zeng","year":"2011","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1104\/pp.98.1.108","article-title":"Solute Accumulation and Compartmentation during the Cold Acclimation of Puma Rye","volume":"98","author":"Koster","year":"1992","journal-title":"Plant Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1105\/tpc.107.056507","article-title":"\u03b2-AMYLASE4, a Noncatalytic Protein Required for Starch Breakdown, Acts Upstream of Three Active \u03b2-Amylases in Arabidopsis Chloroplasts","volume":"20","author":"Fulton","year":"2008","journal-title":"Plant Cell"},{"key":"ref_32","unstructured":"Liang, G. (2022). Study on the Mechanism of Cold Resistance via \u03b2-Amylase Regulating Sugar Metabolism in Grapes. [Ph.D. Dissertation, Gansu Agricultural University]."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2754","DOI":"10.1111\/pce.12384","article-title":"PtrBAM1, a \u03b2-amylase-coding gene of Poncirus trifoliata, is a CBF regulon member with function in cold tolerance by modulating soluble sugar levels","volume":"37","author":"Peng","year":"2014","journal-title":"Plant Cell Environ."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2317","DOI":"10.1093\/jxb\/erx076","article-title":"Amylases StAmy23, StBAM1 and StBAM9 regulate cold-induced sweetening of potato tubers in distinct ways","volume":"68","author":"Hou","year":"2017","journal-title":"J. Exp. Bot."},{"key":"ref_35","unstructured":"Zhao, L. (2019). Bioinformatic Analysis of Pear \u03b2-amylase Family and Identification of PbBAM3 Gene Cold Resistance. [Master\u2019s Thesis, Nanjing Agricultural University]."},{"key":"ref_36","first-page":"1849","article-title":"Advances in RNA Sequencing in Response to Low Temperature Stress in Plants","volume":"18","author":"Zhang","year":"2020","journal-title":"Mol. Plant Breed."},{"key":"ref_37","first-page":"24","article-title":"Identification of sucrose synthase gene family in cabbage and expression pattern analysis in response to low temperature stress","volume":"49","author":"Zhang","year":"2021","journal-title":"Jiangsu Agric. Sci."},{"key":"ref_38","unstructured":"Zhang, Z.L., Qu, W.J., and Li, X.F. (2009). Determination of carbohydrate contents (soluble sugar, sucrose, glucose, fructose and starch). Experimental Guide for Plant Physiology, Higher Education Press. [4th ed.]."},{"key":"ref_39","unstructured":"Zhou, K. (2020). Transcriptome Analysis and Research of Related Gene Function of Starch Accumulation in Soybean Chloroplast Under Salt Stress. [Master\u2019s Thesis, Zhejiang University]."},{"key":"ref_40","first-page":"126","article-title":"The Dewaxing Method of Chinese-Fir Leaf Preparation SEM","volume":"11","author":"You","year":"1991","journal-title":"J. Fujian Coll. For."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_42","unstructured":"Deng, W., and Zhang, Y. (2007). A Review on Real-time Q-PCR Technology. Biotechnol. Bull., 93\u201395+103."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2534\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T04:20:23Z","timestamp":1787458823000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,21]]},"references-count":42,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["plants15162534"],"URL":"https:\/\/doi.org\/10.3390\/plants15162534","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,21]]}}}