{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T04:20:10Z","timestamp":1787545210172,"version":"build-2736575974"},"reference-count":34,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Upland cotton (Gossypium hirsutum, AD1) is the most important fiber crop for the world\u2019s textile industry. The genetic base of Upland cotton is narrow, and the introgression of germplasm from other species into Upland cotton can broaden its genetic diversity, including the introduction of new qualitative traits. The objectives of this study were to determine the inheritance, allelism, and linkage relationships of the red anther trait introduced into Upland from the red anther donor G. armourianum (D2-1) through crosses involving a synthetic tetraploid A1D2-1. After two generations of backcrossing to Upland cotton, followed by inbreeding, homozygous red-anthered plants (RA) were selected and used for crosses with two Upland lines, the Upland standard TM-1, the multiple recessive marker line T582, and the multiple dominant marker line T586. All Upland cotton parents, except the RA parents and T586 (possessing yellow anthers), had cream anthers. The anther color of the F1 plants was red, indicating that the red anther trait is dominant. In nine of ten F2 populations and four of five testcross populations, the segregation of anther color followed a 3:1 and 1:1 ratio, respectively, as expected under a one-gene model. In three F2 populations with a total of 885 plants between RA with green leaf and T586 with red leaf (R1 on chromosome D07), no double recessive genotype (yellow\/cream anther and green leaf) was observed, indicating the allelic relationship between R1 and red anther allele, designated R1a. The recombination frequency between R1a and cluster-1 (cl1) on D07 was estimated to be 0.112 in 256 F2 plants of a cross between RA and T582 based on the maximum likelihood estimation. This new red anther trait and its associated new gene allele provide an important genetic source for understanding the biosynthesis of anthocyanins and may have utility for improving resistance to anther-feeding insects in cotton.<\/jats:p>","DOI":"10.3390\/plants15162544","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:20:36Z","timestamp":1787322036000},"page":"2544","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Analysis of the Red Anther Trait Introgressed into Upland Cotton (Gossypium hirsutum) from Gossypium armourianum"],"prefix":"10.3390","volume":"15","author":[{"given":"Jinfa","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Agriculture, Tennessee Tech University, Cookeville, TN 38505, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James McDonald","family":"Stewart","sequence":"additional","affiliation":[{"name":"Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"ref_1","first-page":"e008","article-title":"Technological advancements in cotton agronomy: A review and prospects","volume":"4","author":"Adeleke","year":"2024","journal-title":"Technol. Agron."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"601","DOI":"10.2135\/cropsci2007.01.0049","article-title":"Genetic gain in yield potential of upland cotton under varying plant densities","volume":"48","author":"Schwartz","year":"2008","journal-title":"Crop Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Smith, S., Diers, B., Specht, J., and Carver, B. (2014). Yield gains in major, U.S. field crops. Cotton, ASA-CSSA-SSSA. CSSA Special Publications.","DOI":"10.2135\/cssaspecpub33"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e70192","DOI":"10.1002\/csc2.70192","article-title":"Genetic gain of lint yield in modern upland cotton cultivars based on national cotton variety tests","volume":"65","author":"Zeng","year":"2025","journal-title":"Crop Sci."},{"key":"ref_5","unstructured":"Constable, G.A., and Forrester, N.W. (1994). Potential for crop improvement with exotic germplasm and genetic engineering. Challenging the Future: Proceedings of the World Cotton Research Conference-1, CSIRO."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10681-014-1094-4","article-title":"Introgression genetics and breeding between Upland and Pima cotton\u2014A review","volume":"198","author":"Zhang","year":"2014","journal-title":"Euphytica"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1002\/plr2.20119","article-title":"TAM KJ-Q14 ESU and TAM 12J-39 ESU upland cotton germplasm with improved fiber bundle strength","volume":"15","author":"Smith","year":"2021","journal-title":"J. Plant Regist."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/07352689.2022.2156061","article-title":"Evolution of the cotton genus, Gossypium, and its domestication in the Americas","volume":"42","author":"Viot","year":"2023","journal-title":"Crit. Rev. Plant Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Arslanova, S.K., Ernazarova, Z.A., Ernazarova, D.K., Turaev, O.S., Safiullina, A.K., Toshpulatov, A.K., Kholova, M.D., Azimova, L.A., Rafiyeva, F.U., and Gapparov, B.M. (2025). Development and characterization of synthetic allotetraploids between diploid species Gossypium herbaceum and Gossypium nelsonii for cotton genetic improvement. Plants, 14.","DOI":"10.3390\/plants14111620"},{"key":"ref_10","unstructured":"Stewart, J.M., and Miles, R.W. (1993). Observations on the genetics of the red anther trait. Proceedings Beltwide Cotton Conferences, National Cotton Council."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kohel, R.J., and Lewis, C.F. (1984). Qualitative genetics, cytology, and cytogenetics. Cotton, ASA-CSSA-SSSA. Agronomy Monographs.","DOI":"10.2134\/agronmonogr24"},{"key":"ref_12","unstructured":"Smith, C.W., and Cothren, J.T. (1999). Qualitative genetics. Cotton, John Wiley and Son. Origin, History, Technology, and Production."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zhang, J.F., Kole, C., and Zhu, Y. (2025). Linkage mapping of qualitative traits and identification of candidate genes in cotton. The Cotton Genome, Springer.","DOI":"10.1007\/978-3-031-84605-2_4"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"167","DOI":"10.2135\/cropsci1961.0011183X000100030004x","article-title":"Origin and inheritance of nectariless cotton","volume":"1","author":"Meyer","year":"1961","journal-title":"Crop Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"28","DOI":"10.2135\/cropsci1981.0011183X002100010008x","article-title":"Nectariless cotton: Effect on growth, survival, and fecundity of lygus bugs","volume":"21","author":"Benedict","year":"1981","journal-title":"Crop Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s10681-020-02630-w","article-title":"Bacterial blight resistance in cotton: Genetic basis and molecular mapping","volume":"216","author":"Zhang","year":"2020","journal-title":"Euphytica"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"108","DOI":"10.22244\/rheedea.1992.02.02.04","article-title":"A revised taxonomic interpretation of Gossypium L. (Malvaceae)","volume":"2","author":"Fryxell","year":"1992","journal-title":"Rheedea"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"146","DOI":"10.5958\/0974-4517.2016.00023.9","article-title":"Development and characterization of an interspecific Gossypium hirsutum \u00d7 Gossypium armourianum hybrid","volume":"18","author":"Kaur","year":"2016","journal-title":"Appl. Biol. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s12600-021-00963-3","article-title":"Morpho-anatomical characterization of interspecific derivatives of Gossypium hirsutum L. \u00d7 G. armourianum Kearney cross for whitefly tolerance","volume":"50","author":"Suthar","year":"2022","journal-title":"Phytoparasitica"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1094\/PDIS-08-24-1645-SC","article-title":"Introgression and mapping of cotton leaf curl disease resistance from wild Gossypium armourianum Kearney into Upland cotton (G. hirsutum)","volume":"109","author":"Pathak","year":"2025","journal-title":"Plant Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/s41437-023-00594-w","article-title":"Comparative transmission genetics of introgressed chromatin in reciprocal advanced backcross populations in Gossypium (cotton) polyploids","volume":"130","author":"Adhikari","year":"2023","journal-title":"Heredity"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1007\/s00438-018-1525-3","article-title":"Over-expression of the red plant gene R1 enhances anthocyanin production and resistance to bollworm and spider mite in cotton","volume":"294","author":"Li","year":"2019","journal-title":"Mol. Genet. Genom."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1093\/plphys\/kiac118","article-title":"Re enhances anthocyanin and proanthocyanidin accumulation to produce red foliated cotton and brown fiber","volume":"189","author":"Wang","year":"2022","journal-title":"Plant Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1007\/s00438-020-01708-3","article-title":"Up-regulation of GhPAP1A results in moderate anthocyanin accumulation and pigmentation in sub-red cotton","volume":"295","author":"Liang","year":"2020","journal-title":"Mol. Genet. Genom."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2000","DOI":"10.1016\/j.jia.2022.10.007","article-title":"A 2-bp frameshift deletion at GhDR, which encodes a B-BOX protein that co-segregates with the dwarf-red phenotype in Gossypium hirsutum L.","volume":"22","author":"Wang","year":"2023","journal-title":"J. Integr. Agric."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100054","DOI":"10.1016\/j.ncrops.2024.100054","article-title":"Perspectives on developing natural colored cotton through carotenoid biofortification","volume":"2","author":"Wang","year":"2025","journal-title":"New Crops"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"100110","DOI":"10.1016\/j.ncrops.2026.100110","article-title":"Insights into the regulatory pattern classification for functional alleles underlying trait variation in maize and other crops","volume":"3","author":"Zhu","year":"2026","journal-title":"New Crops"},{"key":"ref_28","unstructured":"Stewart, J.M. (2008). Evaluation of a red anther trait of Gossypium armourianum introgressed into Gossypium hirsutum. Faculty Impact Statements, University of Arkansas."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11816-015-0382-3","article-title":"Anthocyanin accumulation enhanced in Lc-transgenic cotton under light and increased resistance to bollworm","volume":"10","author":"Fan","year":"2015","journal-title":"Plant Biotechnol. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/BF00387547","article-title":"In-ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.)","volume":"137","author":"Stewart","year":"1977","journal-title":"Planta"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"670","DOI":"10.2135\/cropsci1970.0011183X001000060019x","article-title":"Texas Marker-1. Description of a genetic standard for Gossypium hirsutum L.","volume":"10","author":"Kohel","year":"1970","journal-title":"Crop Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"582","DOI":"10.2135\/cropsci1965.0011183X000500060029x","article-title":"Linkage tests in Upland cotton, Gossypium hirsutum L.","volume":"5","author":"Kohel","year":"1965","journal-title":"Crop Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e2406111","DOI":"10.1002\/advs.202406111","article-title":"A CC-NB-ARC-LRR gene regulates bract morphology in cotton","volume":"11","author":"Yan","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_34","unstructured":"Liu, B. (1998). Statistical Genomics: Linkage, Mapping, and QTL Analysis, CRC Press."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2544\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T04:13:26Z","timestamp":1787544806000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2544"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,21]]},"references-count":34,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["plants15162544"],"URL":"https:\/\/doi.org\/10.3390\/plants15162544","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,21]]}}}