{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T16:45:02Z","timestamp":1787330702853,"version":"build-2736575974"},"reference-count":45,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hainan Provincial Natural Science Foundation of China","award":["325QN336"],"award-info":[{"award-number":["325QN336"]}]},{"name":"Hainan Provincial Natural Science Foundation of China","award":["326MS0191"],"award-info":[{"award-number":["326MS0191"]}]},{"name":"Scientific Research Foundation of Hainan Tropical Ocean University","award":["No. RHDRC202321"],"award-info":[{"award-number":["No. RHDRC202321"]}]},{"award":["No. RHDRC202321"],"award-info":[{"award-number":["No. RHDRC202321"]}],"id":[{"id":"https:\/\/ror.org\/01y5fjx51","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Yuelushan Major Project in Modern Seed Industry","award":["YLS-2025-ZY01008"],"award-info":[{"award-number":["YLS-2025-ZY01008"]}]},{"name":"Scientific research grant of Hunan Agricultural University","award":["25SF019"],"award-info":[{"award-number":["25SF019"]}]},{"award":["25SF019"],"award-info":[{"award-number":["25SF019"]}],"id":[{"id":"https:\/\/ror.org\/01dzed356","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>As a major fungal pathogen of Brassica napus, Sclerotinia sclerotiorum causes significant yield reductions worldwide. Receptor-like proteins (RLPs) are essential components of plant immunity, but the functions of many RLPs in B. napus still remain unclear. In this study, three BnaSOBIR1-interacting leucine-rich repeat RLPs (LRR-RLPs), named BnaRLP-G13-2, BnaRLP-G13-3, and BnaRLP-G13-4, were identified by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. Promoter analysis revealed diverse cis-acting elements involved in stress and phytohormone responses. Overexpression of these genes significantly improved resistance to S. sclerotiorum in both Arabidopsis thaliana and B. napus. Consistently, BnaRLP-G13-2\/3\/4 restored disease resistance and NLP-induced ROS production in the rlp23-1 mutant. Furthermore, BiFC assays suggested an association between BnaRLP-G13-2\/3\/4 and Ssnlp24SsNEP2-associated perception, although direct biochemical binding remains to be demonstrated. These findings advance the understanding of BnaSOBIR1-associated BnaRLP-G13-2\/3\/4-mediated immunity and provide new insights into enhancing disease resistance in oilseed crops.<\/jats:p>","DOI":"10.3390\/plants15162545","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T15:51:05Z","timestamp":1787327465000},"page":"2545","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multiple BnaSOBIR1-Associated Receptor-like Proteins Contribute to Enhanced Resistance to Sclerotinia sclerotiorum in Brassica napus L. (Oilseed Rape)"],"prefix":"10.3390","volume":"15","author":[{"given":"Chenghuizi","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Agriculture and Forestry, Hainan Tropical Ocean University, Sanya 572022, China"},{"name":"Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Forestry, Hainan Tropical Ocean University, Sanya 572022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieying","family":"Nong","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8838-1754","authenticated-orcid":false,"given":"Shitou","family":"Xia","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/nature05286","article-title":"The plant immune system","volume":"444","author":"Jones","year":"2006","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.1111\/pbi.14332","article-title":"PTI-ETI synergistic signal mechanisms in plant immunity","volume":"22","author":"Yu","year":"2024","journal-title":"Plant Biotechnol. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1038\/s41586-021-03315-7","article-title":"Mutual potentiation of plant immunity by cell-surface and intracellular receptors","volume":"592","author":"Ngou","year":"2021","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/s41586-021-03316-6","article-title":"Pattern-recognition receptors are required for NLR-mediated plant immunity","volume":"592","author":"Yuan","year":"2021","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.molp.2014.12.022","article-title":"Signaling mechanisms in pattern-triggered immunity (PTI)","volume":"8","author":"Bigeard","year":"2015","journal-title":"Mol. Plant"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e70167","DOI":"10.1111\/mpp.70167","article-title":"Emerging Roles of Receptor-Like Proteins in Plant Immunity: Crosstalk, Signalling Networks and Prospects for Disease Resistance Breeding","volume":"26","author":"Gao","year":"2025","journal-title":"Mol. Plant Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1007\/s11427-025-3110-3","article-title":"Unraveling plant immunity: From pathogen perception to resistance engineering","volume":"69","author":"Liu","year":"2025","journal-title":"Sci. China Life Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"uhaf082","DOI":"10.1093\/hr\/uhaf082","article-title":"Salicylic acid and jasmonic acid in plant immunity","volume":"12","author":"Zhang","year":"2025","journal-title":"Hortic. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1111\/jipb.13820","article-title":"Salicylic acid: The roles in plant immunity and crosstalk with other hormones","volume":"67","author":"Tian","year":"2025","journal-title":"J. Integr. Plant Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"10010","DOI":"10.1073\/pnas.1220015110","article-title":"Receptor-like kinase SOBIR1\/EVR interacts with receptor-like proteins in plant immunity against fungal infection","volume":"110","author":"Liebrand","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1104\/pp.113.230698","article-title":"Fungal endopolygalacturonases are recognized as microbe-associated molecular patterns by the arabidopsis receptor-like protein RESPONSIVENESS TO BOTRYTIS POLYGALACTURONASES1","volume":"164","author":"Zhang","year":"2014","journal-title":"Plant Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"15140","DOI":"10.1038\/nplants.2015.140","article-title":"An RLP23-SOBIR1-BAK1 complex mediates NLP-triggered immunity","volume":"1","author":"Albert","year":"2015","journal-title":"Nat. Plants"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4227","DOI":"10.1105\/tpc.113.117010","article-title":"Arabidopsis receptor-like protein30 and receptor-like kinase suppressor of BIR1-1\/EVERSHED mediate innate immunity to necrotrophic fungi","volume":"25","author":"Zhang","year":"2013","journal-title":"Plant Cell"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1038\/s41467-018-03010-8","article-title":"Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection","volume":"9","author":"Wang","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"100301","DOI":"10.1016\/j.xplc.2022.100301","article-title":"Structural analysis of receptor-like kinase SOBIR1 reveals mechanisms that regulate its phosphorylation-dependent activation","volume":"3","author":"Wei","year":"2022","journal-title":"Plant Commun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/s41586-021-03829-0","article-title":"The EDS1-PAD4-ADR1 node mediates Arabidopsis pattern-triggered immunity","volume":"598","author":"Pruitt","year":"2021","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"eadt2315","DOI":"10.1126\/sciadv.adt2315","article-title":"Reciprocal phosphorylation between SOAK1 and SOBIR1 fine-tunes receptor-like protein (RLP)-mediated plant immunity","volume":"11","author":"Chen","year":"2025","journal-title":"Sci. Adv."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Yang, K., Chen, C., Wang, Y., Li, J., Dong, X., Cheng, Y., Zhang, H., Zhai, Y., Ai, G., and Song, Q. (2022). Nep1-Like Proteins From the Biocontrol Agent Pythium oligandrum Enhance Plant Disease Resistance Independent of Cell Death and Reactive Oxygen Species. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.830636"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1111\/mpp.13275","article-title":"Heterologous expression of Arabidopsis pattern recognition receptor RLP23 increases broad-spectrum resistance in poplar to fungal pathogens","volume":"24","author":"Zhao","year":"2023","journal-title":"Mol. Plant Pathol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Yang, Y., Steidele, C.E., Huang, X., Loffelhardt, B., Huang, L., Jones, J.D.G., Nurnberger, T., Ding, P., and Gust, A.A. (2025). Engineered pattern recognition receptors enhance broad-spectrum plant resistance. Nat. Biotechnol., 1\u20137.","DOI":"10.1038\/s41587-025-02858-8"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"101072","DOI":"10.1016\/j.xplc.2024.101072","article-title":"Fine-tuning of the dual-role transcription factor WRKY8 via differential phosphorylation for robust broad-spectrum plant immunity","volume":"5","author":"Ren","year":"2024","journal-title":"Plant Commun."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Yang, C., Zhong, W., Li, W., Xia, Y., Qin, L., Tang, X., and Xia, S. (2025). LRR Receptor-like Protein in Rapeseed Confers Resistance to Sclerotinia sclerotiorum Infection via a Conserved SsNEP2 Peptide. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26104569"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3417","DOI":"10.1111\/pbi.70139","article-title":"The receptor-like protein RXEG1 confers broad-spectrum resistance in soybean and cotton by recognizing microbial glycoside hydrolase 12 proteins","volume":"23","author":"Zeng","year":"2025","journal-title":"Plant Biotechnol. J."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Yang, C., Li, W., Huang, X., Tang, X., Qin, L., Liu, Y., Xia, Y., Peng, Z., and Xia, S. (2022). SsNEP2 Contributes to the Virulence of Sclerotinia sclerotiorum. Pathogens, 11.","DOI":"10.3390\/pathogens11040446"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Li, W., Lu, J., Yang, C., and Xia, S. (2022). Identification of receptor-like proteins induced by Sclerotinia sclerotiorum in Brassica napus. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.944763"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.tplants.2024.03.012","article-title":"Immune signaling: Receptor-like proteins make the difference","volume":"30","author":"Huang","year":"2025","journal-title":"Trends Plant Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/jipb.13051","article-title":"Plant plasma membrane-resident receptors: Surveillance for infections and coordination for growth and development","volume":"63","author":"He","year":"2021","journal-title":"J. Integr. Plant Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6697","DOI":"10.1093\/jxb\/erac333","article-title":"Tissue-specific mRNA profiling of the Brassica napus-Sclerotinia sclerotiorum interaction uncovers novel regulators of plant immunity","volume":"73","author":"Walker","year":"2022","journal-title":"J. Exp. Bot."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5042","DOI":"10.1021\/acs.jafc.4c10185","article-title":"Ectopic Overexpression of Rapeseed BnaNTL1 Transcription Factor Positively Regulates Plant Resistance to Sclerotinia sclerotiorum through Modulating JA Synthesis and ROS Accumulation","volume":"73","author":"Li","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"10021","DOI":"10.1038\/s41467-025-65934-2","article-title":"A fungal pathogen manipulates phytocytokine signaling for plant infection","volume":"16","author":"Hua","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e13423","DOI":"10.1111\/mpp.13423","article-title":"The schizotrophic lifestyle of Sclerotinia sclerotiorum","volume":"25","author":"Shang","year":"2024","journal-title":"Mol. Plant Pathol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1038\/s41467-022-29788-2","article-title":"A fungal extracellular effector inactivates plant polygalacturonase-inhibiting protein","volume":"13","author":"Wei","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1111\/mpp.12922","article-title":"An effector of a necrotrophic fungal pathogen targets the calcium-sensing receptor in chloroplasts to inhibit host resistance","volume":"21","author":"Tang","year":"2020","journal-title":"Mol. Plant Pathol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9391","DOI":"10.1038\/s41467-024-53725-0","article-title":"An effector essential for virulence of necrotrophic fungi targets plant HIRs to inhibit host immunity","volume":"15","author":"Liu","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"11034","DOI":"10.1073\/pnas.1606004113","article-title":"Clathrin-dependent endocytosis is required for immunity mediated by pattern recognition receptor kinases","volume":"113","author":"Mbengue","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1093\/pcp\/pcae123","article-title":"Recent Advances in Ubiquitin Signals Regulating Plant Membrane Trafficking","volume":"65","author":"Hasegawa","year":"2024","journal-title":"Plant Cell Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.tibs.2025.10.008","article-title":"Regulation of plant NLRs by post-translational modifications","volume":"51","author":"Zhong","year":"2026","journal-title":"Trends Biochem. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1111\/nph.13802","article-title":"Avr4 promotes Cf-4 receptor-like protein association with the BAK1\/SERK3 receptor-like kinase to initiate receptor endocytosis and plant immunity","volume":"210","author":"Postma","year":"2016","journal-title":"New Phytol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2260","DOI":"10.1111\/nph.16995","article-title":"A receptor-like protein from Nicotiana benthamiana mediates VmE02 PAMP-triggered immunity","volume":"229","author":"Nie","year":"2021","journal-title":"New Phytol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1126\/science.1253435","article-title":"Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome","volume":"345","author":"Chalhoub","year":"2014","journal-title":"Science"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e57495","DOI":"10.15252\/embr.202357495","article-title":"NLR receptors in plant immunity: Making sense of the alphabet soup","volume":"24","author":"Contreras","year":"2023","journal-title":"EMBO Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1093\/bioinformatics\/btu817","article-title":"GSDS 2.0: An upgraded gene feature visualization server","volume":"31","author":"Hu","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/nar\/30.1.325","article-title":"PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences","volume":"30","author":"Lescot","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.molp.2020.06.009","article-title":"TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data","volume":"13","author":"Chen","year":"2020","journal-title":"Mol. Plant."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.ab.2010.05.032","article-title":"A survey of quantitative real-time polymerase chain reaction internal reference genes for expression studies in Brassica napus","volume":"405","author":"Chen","year":"2010","journal-title":"Anal. Biochem."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2545\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T16:21:47Z","timestamp":1787329307000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/16\/2545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,21]]},"references-count":45,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["plants15162545"],"URL":"https:\/\/doi.org\/10.3390\/plants15162545","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,21]]}}}