{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T18:23:39Z","timestamp":1787595819823,"version":"build-2736575974"},"reference-count":63,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fujian Provincial Key Laboratory of Agricultural Genetic Engineering","award":["KFJJ20250408"],"award-info":[{"award-number":["KFJJ20250408"]}]},{"name":"Fujian Provincial Key Laboratory of Agricultural Genetic Engineering","award":["KFJJ20260330"],"award-info":[{"award-number":["KFJJ20260330"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302595"],"award-info":[{"award-number":["32302595"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32302595"],"award-info":[{"award-number":["32302595"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018914","name":"Fujian Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["GJYS202407"],"award-info":[{"award-number":["GJYS202407"]}],"id":[{"id":"10.13039\/501100018914","id-type":"DOI","asserted-by":"publisher"}]},{"award":["GJYS202407"],"award-info":[{"award-number":["GJYS202407"]}],"id":[{"id":"https:\/\/ror.org\/02aj8qz21","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Auxin response factors (ARFs) are key transcriptional regulators of the auxin signaling pathway and play important roles in plant organogenesis and regeneration. However, the functions of ARF family genes in lily scale-derived bulblet regeneration remain largely unclear. In this study, 24 LdARF genes were identified from the genome of Lilium davidii var. unicolor. Phylogenetic analysis revealed that LdARF proteins showed evolutionary conservation with ARF homologs from other monocot species. Genome-wide identification, phylogenetic analysis, and expression profiling revealed functional divergence among LdARF genes during scale-derived bulblet regeneration. Among them, LdARF17 exhibited a distinct regeneration-associated expression pattern, characterized by rapid induction after scale excision and sustained high expression during subsequent bulblet initiation and formation. Subcellular localization analysis demonstrated that LdARF17 is localized in the nucleus. Transient overexpression of LdARF17 significantly promoted bulblet regeneration and was associated with increased expression of auxin-responsive and regeneration-related genes, including IAA14, LBD16, and LBD29. These findings suggest that LdARF17 acts as a positive regulator of lily scale regeneration and may influence auxin-responsive transcriptional processes associated with early cell proliferation, providing new insights into the molecular mechanisms underlying vegetative regeneration in lilies.<\/jats:p>","DOI":"10.3390\/plants15172581","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:20:28Z","timestamp":1787592028000},"page":"2581","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-Wide Identification of the LdARF Gene Family in Lilium davidii var. unicolor and Transient Functional Analysis of LdARF17 in Bulblet Regeneration"],"prefix":"10.3390","volume":"15","author":[{"given":"Ying","family":"Tang","sequence":"first","affiliation":[{"name":"Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Fujian Provincial Key Laboratory of Agricultural Genetic Engineering, Biotechnology Research Institute, Fujian Academy of Agricultural Science, Fuzhou 350003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqing","family":"Wang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Agricultural Genetic Engineering, Biotechnology Research Institute, Fujian Academy of Agricultural Science, Fuzhou 350003, China"},{"name":"Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxin","family":"Gong","sequence":"additional","affiliation":[{"name":"Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Fujian Provincial Key Laboratory of Agricultural Genetic Engineering, Biotechnology Research Institute, Fujian Academy of Agricultural Science, Fuzhou 350003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Guo","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Agricultural Genetic Engineering, Biotechnology Research Institute, Fujian Academy of Agricultural Science, Fuzhou 350003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaozhong","family":"Fang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Agricultural Genetic Engineering, Biotechnology Research Institute, Fujian Academy of Agricultural Science, Fuzhou 350003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5822-3691","authenticated-orcid":false,"given":"Xiaoping","family":"Xu","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Agricultural Genetic Engineering, Biotechnology Research Institute, Fujian Academy of Agricultural Science, Fuzhou 350003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenglong","family":"Yang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Agricultural Genetic Engineering, Biotechnology Research Institute, Fujian Academy of Agricultural Science, Fuzhou 350003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8523-621X","authenticated-orcid":false,"given":"Zhongxiong","family":"Lai","sequence":"additional","affiliation":[{"name":"Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"R545","DOI":"10.1016\/j.cub.2026.04.034","article-title":"The origin and evolution of auxin as a plant signaling molecule","volume":"36","author":"Weijers","year":"2026","journal-title":"Curr. 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