{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T13:23:42Z","timestamp":1787664222312,"version":"build-2736575974"},"reference-count":106,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UAM-Iztapalapa","award":["PE012"],"award-info":[{"award-number":["PE012"]}]},{"name":"UAM-Iztapalapa","award":["7157\/ 2024ECON"],"award-info":[{"award-number":["7157\/ 2024ECON"]}]},{"award":["PE012"],"award-info":[{"award-number":["PE012"]}],"id":[{"id":"https:\/\/ror.org\/02kta5139","id-type":"ROR","asserted-by":"publisher"}]},{"award":["7157\/ 2024ECON"],"award-info":[{"award-number":["7157\/ 2024ECON"]}],"id":[{"id":"https:\/\/ror.org\/02kta5139","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Antimicrobial resistance has intensified the search for novel antimicrobial and wound-healing agents. Ludwigia octovalvis (Jacq.) P.H. Raven is traditionally used to treat infections, inflammation, and skin disorders, although its in vitro biotechnological potential remains largely unexplored. This study reports the first successful establishment of L. octovalvis callus cultures, their characterization by gas chromatography-mass spectrometry (GC-MS), and the evaluation of their antimicrobial and wound-healing activities. Friable calluses were induced from leaf and node explants of axenic seedlings using combinations of 6-benzylaminopurine (BAP), kinetin (KIN), 2,4-dichlorophenoxyacetic acid (2,4-D), and \u03b1-naphthaleneacetic acid (NAA). Optimal callus induction was achieved in leaf explants cultured on full-strength Murashige and Skoog (MS) medium supplemented with BAP (4.44 \u00b5M) and 2,4-D (0.45 \u00b5M). After six months of subculture, the calluses showed morphological uniformity and stable biomass production. Growth kinetics followed a specific growth rate of 0.047 d\u22121 and a doubling time of 14.54 days. GC-MS analysis identified fatty acids and phytosterols as the main components. The ethyl acetate extract significantly improved wound healing in vivo, while the methanolic extract exhibited antibacterial activity against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA). These findings demonstrate that L. octovalvis callus cultures constitute a sustainable source of bioactive compounds with promising therapeutic potential.<\/jats:p>","DOI":"10.3390\/plants15172590","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T12:57:17Z","timestamp":1787662637000},"page":"2590","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Callus Induction in Ludwigia octovalvis: Chemical Characterization and Potential Pharmacological Applications"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2697-0961","authenticated-orcid":false,"given":"Stephany Abigail","family":"Tadeo-Cuenca","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Metropolitan Autonomous University-Iztapalapa Campus, Av. Ferrocarril de San Rafael Atlixco 186, Col. Leyes de Reforma 1\u00aa. Secci\u00f3n, Alcald\u00eda Iztapalapa, Mexico City 09310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5082-9798","authenticated-orcid":false,"given":"Silvia","family":"Marquina-Bahena","sequence":"additional","affiliation":[{"name":"Chemical Research Center-IICBA, Autonomous University of the State of Morelos, Av. Universidad 1001, Col. 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Universidad 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7612-3726","authenticated-orcid":false,"given":"Araceli","family":"Guerrero-Alonso","sequence":"additional","affiliation":[{"name":"Chemical Research Center-IICBA, Autonomous University of the State of Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7127-7264","authenticated-orcid":false,"given":"Francisco","family":"Cruz-Sosa","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Metropolitan Autonomous University-Iztapalapa Campus, Av. Ferrocarril de San Rafael Atlixco 186, Col. Leyes de Reforma 1\u00aa. Secci\u00f3n, Alcald\u00eda Iztapalapa, Mexico City 09310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3558-4803","authenticated-orcid":false,"given":"Mariana","family":"S\u00e1nchez-Ramos","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Metropolitan Autonomous University-Iztapalapa Campus, Av. Ferrocarril de San Rafael Atlixco 186, Col. Leyes de Reforma 1\u00aa. Secci\u00f3n, Alcald\u00eda Iztapalapa, Mexico City 09310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"ref_1","unstructured":"GBD 2021 Antimicrobial Resistance Collaborators (2024). Global Burden of Bacterial Antimicrobial Resistance 1990\u20132021: A Systematic Analysis with Forecasts to 2050. 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