{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T12:17:35Z","timestamp":1787833055978,"version":"build-2784847793"},"reference-count":62,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32300580"],"award-info":[{"award-number":["32300580"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32000368"],"award-info":[{"award-number":["32000368"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32300580"],"award-info":[{"award-number":["32300580"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["32000368"],"award-info":[{"award-number":["32000368"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Protein family membership may constrain protein thermal stability, but the extent of this effect remains unclear. We reanalyzed a published thermal proteome dataset containing 3917 Arabidopsis thaliana proteins. Proteins within the same family showed significantly smaller pairwise Tm differences than size-matched random groups, and family membership explained 67.6% of total Tm variation. Although thermal stability was generally conserved within families, some paralogs displayed marked divergence beyond that expected from sequence similarity. This exceptional divergence was not associated with duplication mode or overall structural distance, whereas differences in disulfide-bond density showed the clearest structural association. Gene Ontology cellular-component divergence was also positively associated with thermal divergence. Overall, thermal stability is strongly organized at the gene-family level, while exceptional divergence among paralogs appears to arise through heterogeneous, pair-specific structural and functional changes.<\/jats:p>","DOI":"10.3390\/plants15172617","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T10:58:21Z","timestamp":1787828301000},"page":"2617","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gene Families, Structural and Functional Contexts Shape Protein Thermostability and Paralog Divergence in Arabidopsis"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1816-8640","authenticated-orcid":false,"given":"Yanli","family":"Cheng","sequence":"first","affiliation":[{"name":"College of Life Sciences, Shanxi Normal University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Shanxi Normal University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Shanxi Normal University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhitao","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Shanxi Normal University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongbo","family":"Han","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Shanxi Normal University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junlong","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Shanxi Normal University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haining","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Shanxi Normal University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weizhong","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Shanxi Normal University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqing","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Shanxi Normal University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1255784","DOI":"10.1126\/science.1255784","article-title":"Tracking cancer drugs in living cells by thermal profiling of the proteome","volume":"346","author":"Savitski","year":"2014","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s00792-007-0089-7","article-title":"The effect of temperature on enzyme activity: New insights and their implications","volume":"12","author":"Daniel","year":"2008","journal-title":"Extremophiles"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1146\/annurev.ph.57.030195.000355","article-title":"Proteins and temperature","volume":"57","author":"Somero","year":"1995","journal-title":"Annu. Rev. Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.cell.2016.08.029","article-title":"Abiotic Stress Signaling and Responses in Plants","volume":"167","author":"Zhu","year":"2016","journal-title":"Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1105\/tpc.12.4.479","article-title":"Heat shock protein 101 plays a crucial role in thermotolerance in Arabidopsis","volume":"12","author":"Queitsch","year":"2000","journal-title":"Plant Cell"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4392","DOI":"10.1073\/pnas.97.8.4392","article-title":"Mutants of Arabidopsis thaliana defective in the acquisition of tolerance to high temperature stress","volume":"97","author":"Hong","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1021\/pr0705340","article-title":"Heat-Shock Response in Arabidopsis thaliana Explored by Multiplexed Quantitative Proteomics Using Differential Metabolic Labeling","volume":"7","author":"Palmblad","year":"2008","journal-title":"J. Proteome Res."},{"key":"ref_8","first-page":"1221","article-title":"Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress1[OPEN]","volume":"172","author":"McLoughlin","year":"2016","journal-title":"Plant Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1038\/nmeth.3652","article-title":"Thermal proteome profiling monitors ligand interactions with cellular membrane proteins","volume":"12","author":"Reinhard","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.cels.2023.08.003","article-title":"Systematic thermal analysis of the Arabidopsis proteome: Thermal tolerance, organization, and evolution","volume":"14","author":"Lyu","year":"2023","journal-title":"Cell Syst."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.1233606","article-title":"Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay","volume":"341","author":"Jafari","year":"2013","journal-title":"Science"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1038\/nmeth.3590","article-title":"Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling","volume":"12","author":"Huber","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1038\/nchembio.2185","article-title":"Thermal profiling reveals phenylalanine hydroxylase as an off-target of panobinostat","volume":"12","author":"Becher","year":"2016","journal-title":"Nat. Chem. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"eaai7825","DOI":"10.1126\/science.aai7825","article-title":"Cell-wide analysis of protein thermal unfolding reveals determinants of thermostability","volume":"355","author":"Leuenberger","year":"2017","journal-title":"Science"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s12953-017-0122-4","article-title":"Thermal proteome profiling: Unbiased assessment of protein state through heat-induced stability changes","volume":"15","author":"Mateus","year":"2016","journal-title":"Proteome Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.cell.2017.12.006","article-title":"A Map of Protein-Metabolite Interactions Reveals Principles of Chemical Communication","volume":"172","author":"Piazza","year":"2018","journal-title":"Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e8242","DOI":"10.15252\/msb.20188242","article-title":"Thermal proteome profiling in bacteria: Probing protein state in vivo","volume":"14","author":"Mateus","year":"2018","journal-title":"Mol. Syst. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4027","DOI":"10.1021\/acs.jproteome.9b00500","article-title":"Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution","volume":"18","author":"Gaetani","year":"2019","journal-title":"J. Proteome Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1038\/s41467-019-09107-y","article-title":"Proteome-wide solubility and thermal stability profiling reveals distinct regulatory roles for ATP","volume":"10","author":"Sridharan","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/s41592-020-0801-4","article-title":"Meltome atlas-thermal proteome stability across the tree of life","volume":"17","author":"Jarzab","year":"2020","journal-title":"Nat. Methods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1038\/s41587-019-0388-4","article-title":"Identifying drug targets in tissues and whole blood with thermal-shift profiling","volume":"38","author":"Perrin","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/s41586-020-3002-5","article-title":"The functional proteome landscape of Escherichia coli","volume":"588","author":"Mateus","year":"2020","journal-title":"Nature"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1126\/science.aan0346","article-title":"Thermal proximity coaggregation for system-wide profiling of protein complex dynamics in cells","volume":"359","author":"Tan","year":"2018","journal-title":"Science"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1016\/j.cell.2018.03.053","article-title":"Pervasive Protein Thermal Stability Variation during the Cell Cycle","volume":"173","author":"Becher","year":"2018","journal-title":"Cell"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.cell.2018.02.030","article-title":"Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis","volume":"173","author":"Savitski","year":"2018","journal-title":"Cell"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.cell.2020.12.021","article-title":"Dynamic 3D proteomes reveal protein functional alterations at high resolution in situ","volume":"184","author":"Cappelletti","year":"2021","journal-title":"Cell"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1074\/mcp.RA118.001124","article-title":"Proteome-wide Analysis of Protein Thermal Stability in the Model Higher Plant Arabidopsis thaliana","volume":"18","author":"Volkening","year":"2019","journal-title":"Mol. Cell Proteom."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.cels.2023.09.001","article-title":"Observations and implication of thermal tolerance in the Arabidopsis proteome","volume":"14","author":"Fernie","year":"2023","journal-title":"Cell Syst."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1093\/genetics\/151.4.1531","article-title":"Preservation of duplicate genes by complementary, degenerative mutations","volume":"151","author":"Force","year":"1999","journal-title":"Genetics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1126\/science.290.5494.1151","article-title":"The evolutionary fate and consequences of duplicate genes","volume":"290","author":"Lynch","year":"2000","journal-title":"Science"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Arabidopsis Genome Initiative (2000). Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature, 408, 796\u2013815.","DOI":"10.1038\/35048692"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1126\/science.290.5499.2114","article-title":"The origins of genomic duplications in Arabidopsis","volume":"290","author":"Vision","year":"2000","journal-title":"Science"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1038\/nature01521","article-title":"Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events","volume":"422","author":"Bowers","year":"2003","journal-title":"Nature"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1105\/tpc.021410","article-title":"Functional divergence of duplicated genes formed by polyploidy during Arabidopsis evolution","volume":"16","author":"Blanc","year":"2004","journal-title":"Plant Cell"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Wang, Y., Wang, X., Tang, H., Tan, X., Ficklin, S.P., Feltus, F.A., and Paterson, A.H. (2011). Modes of Gene Duplication Contribute Differently to Genetic Novelty and Redundancy, but Show Parallels across Divergent Angiosperms. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0028150"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5454","DOI":"10.1073\/pnas.0501102102","article-title":"Modeling gene and genome duplications in eukaryotes","volume":"102","author":"Maere","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"R13","DOI":"10.1186\/gb-2006-7-2-r13","article-title":"Nonrandom divergence of gene expression following gene and genome duplications in the flowering plant Arabidopsis thaliana","volume":"7","author":"Casneuf","year":"2006","journal-title":"Genome Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1093\/molbev\/msj051","article-title":"Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis","volume":"23","author":"Duarte","year":"2006","journal-title":"Mol. Biol. Evol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1093\/molbev\/msm158","article-title":"Divergence in expression between duplicated genes in Arabidopsis","volume":"24","author":"Ganko","year":"2007","journal-title":"Mol. Biol. Evol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1104\/pp.108.122457","article-title":"Importance of lineage-specific expansion of plant tandem duplicates in the adaptive response to environmental stimuli","volume":"148","author":"Hanada","year":"2008","journal-title":"Plant Physiol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Hanada, K., Kuromori, T., Myouga, F., Toyoda, T., and Shinozaki, K. (2009). Increased Expression and Protein Divergence in Duplicate Genes Is Associated with Morphological Diversification. PLoS Genet., 5.","DOI":"10.1371\/journal.pgen.1000781"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e49","DOI":"10.1093\/nar\/gkr1293","article-title":"MCScanX: A toolkit for detection and evolutionary analysis of gene synteny and collinearity","volume":"40","author":"Wang","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Wang, Y., Tan, X., and Paterson, A.H. (2013). Different patterns of gene structure divergence following gene duplication in Arabidopsis. BMC Genom., 14.","DOI":"10.1186\/1471-2164-14-652"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1104\/pp.112.213447","article-title":"Function Relaxation Followed by Diversifying Selection after Whole-Genome Duplication in Flowering Plants","volume":"162","author":"Guo","year":"2013","journal-title":"Plant Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2786","DOI":"10.1105\/tpc.17.00531","article-title":"Coordinated Functional Divergence of Genes after Genome Duplication in Arabidopsis thaliana","volume":"29","author":"Sabaghian","year":"2017","journal-title":"Plant Cell"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1111\/nph.16148","article-title":"Divergence of duplicated genes by repeated partitioning of splice forms and subcellular localization","volume":"225","author":"Qiu","year":"2020","journal-title":"New Phytol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2402","DOI":"10.1093\/gbe\/evt183","article-title":"Protein subcellular relocalization increases the retention of eukaryotic duplicate genes","volume":"5","author":"Byun","year":"2013","journal-title":"Genome Biol. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"R54","DOI":"10.1186\/gb-2008-9-3-r54","article-title":"Functional diversification of duplicate genes through subcellular adaptation of encoded proteins","volume":"9","author":"Marques","year":"2008","journal-title":"Genome Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/S0969-2126(00)00133-7","article-title":"Structural differences between mesophilic, moderately thermophilic and extremely thermophilic protein subunits: Results of a comprehensive survey","volume":"8","year":"2000","journal-title":"Structure"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"8152","DOI":"10.1021\/bi025523t","article-title":"Elucidation of Factors Responsible for Enhanced Thermal Stability of Proteins: A Structural Genomics Based Study","volume":"41","author":"Chakravarty","year":"2002","journal-title":"Biochemistry"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1080\/07391102.2000.10506606","article-title":"Contribution of salt bridges toward protein thermostability","volume":"17","author":"Kumar","year":"2000","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.jmb.2005.11.065","article-title":"Contribution of electrostatic interactions, compactness and quaternary structure to protein thermostability: Lessons from structural genomics of Thermotoga maritima","volume":"356","author":"Godzik","year":"2006","journal-title":"J. Mol. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1093\/protein\/13.11.753","article-title":"Aromatic clusters: A determinant of thermal stability of thermophilic proteins","volume":"13","author":"Kannan","year":"2000","journal-title":"Protein Eng."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1110\/ps.26801","article-title":"Disulfide bond effects on protein stability: Designed variants of Cucurbita maxima trypsin inhibitor-V","volume":"10","author":"Zavodszky","year":"2001","journal-title":"Protein Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1105\/tpc.106.044230","article-title":"An Arabidopsis Glutathione Peroxidase Functions as Both a Redox Transducer and a Scavenger in Abscisic Acid and Drought Stress Responses","volume":"18","author":"Miao","year":"2006","journal-title":"Plant Cell"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1046\/j.1365-313X.2003.01901.x","article-title":"Glutathione peroxidase genes in Arabidopsis are ubiquitous and regulated by abiotic stresses through diverse signaling pathways","volume":"36","author":"Maurer","year":"2003","journal-title":"Plant J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s11103-019-00953-4","article-title":"The Arabidopsis UDP-glycosyltransferase75B1, conjugates abscisic acid and affects plant response to abiotic stresses","volume":"102","author":"Chen","year":"2020","journal-title":"Plant Mol. Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1111\/pce.12221","article-title":"The secondary metabolism glycosyltransferases UGT73B3 and UGT73B5 are components of redox status in resistance of Arabidopsis thaliana to Pseudomonas syringae pv. tomato","volume":"37","author":"Simon","year":"2014","journal-title":"Plant Cell Environ."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1104\/pp.117.1.263","article-title":"Isolation of the ornithine-\u03b4-aminotransferase cDNA and effect of salt stress on its expression in Arabidopsis thaliana","volume":"117","author":"Roosens","year":"1998","journal-title":"Plant Physiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"4623","DOI":"10.1093\/plcell\/koac237","article-title":"Glutamate 1-semialdehyde aminotransferase is connected to GluTR by GluTR-binding protein and contributes to the rate-limiting step of 5-aminolevulinic acid synthesis","volume":"34","author":"Apel","year":"2022","journal-title":"Plant Cell"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.tplants.2004.06.004","article-title":"The ALDH gene superfamily of Arabidopsis","volume":"9","author":"Kirch","year":"2004","journal-title":"Trends Plant Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s13059-019-1650-2","article-title":"Gene duplication and evolution in recurring polyploidization-diploidization cycles in plants","volume":"20","author":"Qiao","year":"2019","journal-title":"Genome Biol."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2617\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T11:18:24Z","timestamp":1787829504000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2617"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,27]]},"references-count":62,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["plants15172617"],"URL":"https:\/\/doi.org\/10.3390\/plants15172617","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,27]]}}}