{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T05:16:25Z","timestamp":1788066985189,"version":"build-2784847793"},"reference-count":51,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan","doi-asserted-by":"crossref","award":["242300421116"],"award-info":[{"award-number":["242300421116"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32201873"],"award-info":[{"award-number":["32201873"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32201873"],"award-info":[{"award-number":["32201873"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"State Key Laboratory of Cotton Bio-breeding and Integrated Utilization Open Fund","award":["CB2024A21"],"award-info":[{"award-number":["CB2024A21"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["1610162026047"],"award-info":[{"award-number":["1610162026047"]}]},{"name":"China Agriculture Research System","award":["CARS-15-06"],"award-info":[{"award-number":["CARS-15-06"]}]},{"award":["CARS-15-06"],"award-info":[{"award-number":["CARS-15-06"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Drought stress is a major abiotic factor limiting cotton yield and quality. RING-type E3 ubiquitin ligases play central regulatory roles in plant stress responses; however, the functions and regulatory networks of this gene family in cotton drought response remain largely unclear. In this study, we characterized the RING-type E3 ubiquitin ligase gene GhRNG1L from upland cotton (Gossypium hirsutum L.) and demonstrated its role in enhancing drought tolerance in cotton, and we successfully identified its upstream transcription factor GhDREB2B. Functional assays showed that overexpression of GhRNG1L significantly increased catalase (CAT) activity and reduced hydrogen peroxide (H2O2) accumulation in Arabidopsis, thereby improving plant drought tolerance, whereas silencing GhRNG1L produced the opposite phenotype. Further molecular analysis confirmed that GhDREB2B directly binds to the promoter of GhRNG1L and activates its transcription. Collectively, our findings reveal, for the first time, the positive regulatory role of the GhDREB2B-GhRNG1L module in cotton drought response, providing a clear upstream regulatory target and theoretical basis for molecular breeding of drought-tolerant cotton.<\/jats:p>","DOI":"10.3390\/plants15172636","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:33:52Z","timestamp":1787942032000},"page":"2636","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Analysis of GhRNG1L Reveals Its Positive Role in Drought Tolerance Mediated by GhDREB2B in Cotton"],"prefix":"10.3390","volume":"15","author":[{"given":"Mengyan","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0893-9118","authenticated-orcid":false,"given":"Li","family":"An","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Xiaokang","family":"Fu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, 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China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Hongmei","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"visualization","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Yanna","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China"},{"name":"National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Life Sciences, Henan University, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3236-9286","authenticated-orcid":false,"given":"Guoli","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Hantao","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"ref_1","first-page":"22","article-title":"Functional analysis of drought tolerance in Gossypium hirsutum L. transformed with HaNAC12 gene","volume":"38","author":"Mo","year":"2026","journal-title":"Cotton Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/pbi.12688","article-title":"Drought coping strategies in cotton: Increased crop per drop","volume":"15","author":"Ullah","year":"2017","journal-title":"Plant Biotechnol. J."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Saud, S., and Wang, L. (2022). Mechanism of cotton resistance to abiotic stress, and recent research advances in the osmoregulation related genes. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.972635"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.indcrop.2018.12.070","article-title":"Progress and Perspective On Drought and Salt Stress Tolerance In Cotton","volume":"130","author":"Abdelraheem","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zhang, L., Ma, H., Chen, T., Peng, J., Yu, S., and Zhao, X. (2014). Morphological and physiological responses of cotton (Gossypium hirsutum L.) plants to salinity. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0112807"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1111\/jac.12389","article-title":"Drought effects on cotton (Gossypium hirsutum L.) fibre quality and fibre sucrose metabolism during the flowering and boll-formation period","volume":"206","author":"Gao","year":"2020","journal-title":"J. Agron. Crop Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Joshi, R., Wani, S.H., Singh, B., Bohra, A., Dar, Z.A., Lone, A.A., Pareek, A., and Singla-Pareek, S.L. (2016). Transcription factors and plant response to drought stress: Current understanding and future directions. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.01029"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/j.1439-037X.2008.00323.x","article-title":"Physiological Role of Exogenously Applied Glycinebetaine to Improve Drought Tolerance in Fine Grain Aromatic Rice (Oryza sativa L.)","volume":"194","author":"Farooq","year":"2008","journal-title":"J. Agron. Crop Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/j.plaphy.2010.08.016","article-title":"Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants","volume":"48","author":"Gill","year":"2010","journal-title":"Plant Physiol. Biochem."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Fahad, S., Bajwa, A.A., Nazir, U., Anjum, S.A., Farooq, A., Zohaib, A., Sadia, S., Nasim, W., Adkins, S., and Saud, S. (2017). Crop Production under Drought and Heat Stress: Plant Responses and Management Options. Front. Plant Sci., 8.","DOI":"10.3389\/fpls.2017.01147"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Al-Saharin, R., Hellmann, H., and Mooney, S. (2022). Plant E3 Ligases and Their Role in Abiotic Stress Response. Cells, 11.","DOI":"10.3390\/cells11050890"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1038\/s41477-023-01510-0","article-title":"Structural basis for abscisic acid efflux mediated by ABCG25 in Arabidopsis thaliana","volume":"9","author":"Wei","year":"2023","journal-title":"Nat. Plants"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.plantsci.2014.06.009","article-title":"Physiological and gene expression responses of sunflower (Helianthus annuus L.) plants differ according to irrigation placement","volume":"227","author":"Aguado","year":"2014","journal-title":"Plant Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1007\/s00299-011-1068-0","article-title":"Plant tolerance to drought and salinity: Stress regulating transcription factors and their functional significance in the cellular transcriptional network","volume":"30","author":"Golldack","year":"2011","journal-title":"Plant Cell Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"D1182","DOI":"10.1093\/nar\/gkt1016","article-title":"PlantTFDB 3.0: A portal for the functional and evolutionary study of plant transcription factors","volume":"42","author":"Jin","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"20240236","DOI":"10.1098\/rstb.2024.0236","article-title":"Transcriptional gene network involved in drought stress response: Application for crop breeding in the context of climate change","volume":"380","author":"Nakashima","year":"2025","journal-title":"Philos. Trans. B"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1093\/jxb\/erz569","article-title":"Overexpression of soybean DREB1 enhances drought stress tolerance of transgenic wheat in the field","volume":"71","author":"Zhou","year":"2020","journal-title":"J. Exp. Bot."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1093\/jxb\/erx118","article-title":"Dehydration responsive element binding transcription factors and their applications for the engineering of stress tolerance","volume":"68","author":"Agarwal","year":"2017","journal-title":"J. Exp. Bot."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zhang, B., Su, L., Hu, B., and Li, L. (2018). Expression of AhDREB1, an AP2\/ERF Transcription Factor Gene from Peanut, Is Affected by Histone Acetylation and Increases Abscisic Acid Sensitivity and Tolerance to Osmotic Stress in Arabidopsis. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19051441"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1146\/annurev-biochem-061516-044916","article-title":"Mechanisms of Deubiquitinase Specificity and Regulation","volume":"86","author":"Mevissen","year":"2017","journal-title":"Annu. Rev. Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/bs.ircmb.2018.05.012","article-title":"Role of the Ubiquitin Proteasome System in Plant Response to Abiotic Stress","volume":"343","author":"Stone","year":"2019","journal-title":"Int. Rev. Cell Mol. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1016\/j.tplants.2018.09.004","article-title":"Protein Language: Post-Translational Modifications Talking to Each Other","volume":"23","author":"Vu","year":"2018","journal-title":"Trends Plant Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1083\/jcb.201902074","article-title":"Post-translational regulation of ubiquitin signaling","volume":"218","author":"Song","year":"2019","journal-title":"J. Cell Biol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Stone, S.L. (2014). The role of ubiquitin and the 26S proteasome in plant abiotic stress signaling. Front. Plant Sci., 5.","DOI":"10.3389\/fpls.2014.00135"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Wang, S., Lv, X., Zhang, J., Chen, D., Chen, S., Fan, G., Ma, C., and Wang, Y. (2022). Roles of E3 Ubiquitin Ligases in Plant Responses to Abiotic Stresses. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23042308"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/978-1-4939-8706-1_6","article-title":"Enzymatic Assembly of Ubiquitin Chains","volume":"1844","author":"Michel","year":"2018","journal-title":"Methods Mol. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1104\/pp.113.220103","article-title":"The Arabidopsis RING E3 ubiquitin ligase AtAIRP3\/LOG2 participates in positive regulation of high-salt and drought stress responses","volume":"162","author":"Kim","year":"2013","journal-title":"Plant Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1093\/jxb\/err310","article-title":"Abiotic stress tolerance mediated by protein ubiquitination","volume":"63","author":"Lyzenga","year":"2012","journal-title":"J. Exp. Bot."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/s12374-012-0902-4","article-title":"Roles of various cullin-RING E3 ligases involved in hormonal and stress responses in plants","volume":"55","author":"Seo","year":"2012","journal-title":"J. Plant Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/pbr.12004","article-title":"Drought stress adaptation: Metabolic adjustment and regulation of gene expression","volume":"132","author":"Bhargava","year":"2013","journal-title":"Plant Breed."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Mahmood, T., Khalid, S., Abdullah, M., Ahmed, Z., Shah, M.K.N., Ghafoor, A., and Du, X. (2019). Insights into Drought Stress Signaling in Plants and the Molecular Genetic Basis of Cotton Drought Tolerance. Cells, 9.","DOI":"10.3390\/cells9010105"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Wang, Y., Zeng, J., Yu, Y., and Ni, Z. (2024). Silencing of GhSINAT5 Reduces Drought Resistance and Salt Tolerance in Cotton. Genes, 15.","DOI":"10.3390\/genes15081063"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s00299-008-0623-9","article-title":"A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat","volume":"28","author":"Gao","year":"2009","journal-title":"Plant Cell Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"kiae580","DOI":"10.1093\/plphys\/kiae580","article-title":"MYB30-INTERACTING E3 LIGASE 1 regulates LONELY GUY 5-mediated cytokinin metabolism to promote drought tolerance in cotton","volume":"197","author":"Chen","year":"2024","journal-title":"Plant Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1105\/tpc.110.081885","article-title":"The Arabidopsis E3 ubiquitin ligase HOS1 negatively regulates CONSTANS abundance in the photoperiodic control of flowering","volume":"24","author":"Lazaro","year":"2012","journal-title":"Plant Cell"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Yue, J., Zou, X., Peng, Y., Pan, S., Hu, C., Wang, B., Dai, L., and Li, W. (2025). The Arabidopsis E3 ubiquitin ligase PUB13 synergistically interacts with BON1 to regulate plant flowering and immunity. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1585221"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Liu, J., Odongo, M.R., Xu, Y., Wei, T., Kirungu, J.N., Zheng, J., Hou, Y., Wang, Y., Agong, G.S., and Okuto, E. (2021). Functional Characterization of Cotton C-Repeat Binding Factor Genes Reveal Their Potential Role in Cold Stress Tolerance. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.766130"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Li, S., Liu, S., Zhang, Q., Cui, M., Zhao, M., Li, N., Wang, S., Wu, R., Zhang, L., and Cao, Y. (2022). The interaction of ABA and ROS in plant growth and stress resistances. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1050132"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1083\/jcb.201708007","article-title":"Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling","volume":"217","author":"Wang","year":"2018","journal-title":"J. Cell Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1111\/jipb.13845","article-title":"Arabidopsis CIRP1 E3 ligase modulates drought and oxidative stress tolerance and reactive oxygen species homeostasis by directly degrading catalases","volume":"67","author":"Yang","year":"2025","journal-title":"J. Integr. Plant Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1038\/s41467-019-09341-4","article-title":"Addendum: Arabidopsis ubiquitin ligase MIEL1 mediates degradation of the transcription factor MYB30 weakening plant defence","volume":"10","author":"Marino","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"12525","DOI":"10.1038\/ncomms12525","article-title":"The Arabidopsis MIEL1 E3 ligase negatively regulates ABA signalling by promoting protein turnover of MYB96","volume":"7","author":"Lee","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1016\/j.tplants.2026.03.003","article-title":"Mapping plant cell-type-specific responses to environmental stresses","volume":"31","author":"Ali","year":"2026","journal-title":"Trends Plant Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e70165","DOI":"10.1111\/ppl.70165","article-title":"Auxin biodynamics and its integral role in enhancing plant resilience to environmental cues","volume":"177","author":"Ali","year":"2025","journal-title":"Physiol. Plant."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"12071","DOI":"10.1038\/srep12071","article-title":"The E3 Ubiquitin Ligase COP1 Regulates Thermosensory Flowering by Triggering GI Degradation in Arabidopsis","volume":"5","author":"Jang","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3228","DOI":"10.1105\/tpc.15.00321","article-title":"Arabidopsis RZFP34\/CHYR1, a Ubiquitin E3 Ligase, Regulates Stomatal Movement and Drought Tolerance via SnRK2.6-Mediated Phosphorylation","volume":"27","author":"Ding","year":"2015","journal-title":"Plant Cell"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"119273","DOI":"10.1016\/j.indcrop.2024.119273","article-title":"Xyloglucan endotransglucosylase-hydrolase 22 positively regulates response to cold stress in upland cotton (Gossypium hirsutum L.)","volume":"220","author":"Wu","year":"2024","journal-title":"Ind. Crops Prod."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"413","DOI":"10.2144\/05383RV01","article-title":"Quantitative imaging of protein interactions in the cell nucleus","volume":"38","author":"Voss","year":"2005","journal-title":"BioTechniques"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3071","DOI":"10.1093\/jxb\/erv111","article-title":"Down-regulation of the cotton endo-1,4-\u03b2-glucanase gene KOR1 disrupts endosperm cellularization, delays embryo development, and reduces early seedling vigour","volume":"66","author":"Shang","year":"2015","journal-title":"J. Exp. Bot."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"610","DOI":"10.2144\/04374ST06","article-title":"Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals","volume":"37","author":"Ballester","year":"2004","journal-title":"BioTechniques"},{"key":"ref_51","first-page":"493","article-title":"Mepiquat chloride improves drought tolerance by adjusting water balance and photosynthetic performance in cotton seedling","volume":"33","author":"Wang","year":"2021","journal-title":"Cotton Sci."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2636\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T04:30:56Z","timestamp":1788064256000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2636"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,28]]},"references-count":51,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["plants15172636"],"URL":"https:\/\/doi.org\/10.3390\/plants15172636","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,28]]}}}