{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:18:37Z","timestamp":1788153517417,"version":"build-2803163510"},"reference-count":72,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Iron (Fe) deficiency and cadmium (Cd) contamination often co-occur in agricultural systems, yet the way in which Fe deficiency modulates Cd translocation remains unclear. Here, we investigated root cell wall modifications mediating Cd accumulation in two peanut cultivars with contrasting Fe deficiency tolerance. Fe deficiency significantly increased root Cd concentrations in both cultivars but reduced Cd translocation to shoots, an effect more pronounced in the tolerant cultivar Silihong. Cell wall analysis revealed cultivar-specific compositional changes: pectin and cellulose increased under combined Cd exposure and Fe deficiency, while hemicellulose (HC1) decreased. Negative correlations between Fe and Cd accumulation in roots, cell walls, and their components indicate competition between these two metal ions for binding sites in root cell walls. Increased pectin content under combined stress enhances Cd sequestration, while reduced HC1 content facilitates Fe mobilization to shoots. Transcriptomic analysis identified hub genes associated with cell wall modification, including pectinesterases (PME2\/4\/29\/63), beta-galactosidases (BGAL3\/5\/8), polygalacturonases (PGs), pectin acetylesterases (PAE8), xyloglucan endotransglucosylase\/hydrolases (XTH8\/31) and laccases (LAC7\/11\/15). Under combined stress, Silihong exhibited superior Cd immobilization, characterized by higher Cd accumulation in HC1 and cellulose fractions, stronger induction of PME, PAE8 and LAC genes, and greater suppression of XTHs, PGs, and BGALs. Our findings demonstrate that Fe deficiency restricts Cd translocation by remodeling root cell walls, increasing pectin and cellulose while modulating hemicellulose integrity, thereby creating an expanded apoplastic reservoir that traps Cd. This structural detoxification mechanism, operating downstream of uptake transporters, identifies key cell wall components and regulatory genes as potential targets for breeding peanut cultivars with improved food safety.<\/jats:p>","DOI":"10.3390\/plants15172641","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:33:52Z","timestamp":1787942032000},"page":"2641","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Iron Deficiency Reduces Cadmium Translocation in Peanut by Increasing the Root Cell Wall Reservoir"],"prefix":"10.3390","volume":"15","author":[{"given":"Rui","family":"Liu","sequence":"first","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyue","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4481-2580","authenticated-orcid":false,"given":"Gangrong","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"111887","DOI":"10.1016\/j.ecoenv.2020.111887","article-title":"Cadmium Toxicity in Plants: Impacts and Remediation Strategies","volume":"211","author":"Haider","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1080\/07352689.2020.1792179","article-title":"Mechanisms of Cadmium Accumulation in Plants","volume":"39","author":"Sterckeman","year":"2020","journal-title":"Crit. Rev. Plant Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1093\/jxb\/erab003","article-title":"All Together Now: Regulation of the Iron Deficiency Response","volume":"72","author":"Riaz","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1002\/csc2.20661","article-title":"Iron Deficiency in Soybean","volume":"62","author":"Merry","year":"2022","journal-title":"Crop Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0140-6736(20)32594-0","article-title":"Iron Deficiency","volume":"397","author":"Pasricha","year":"2021","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"105155","DOI":"10.1016\/j.envexpbot.2022.105155","article-title":"Cadmium-Induced Iron Deficiency Is a Compromise Strategy to Reduce Cd Uptake in Rice","volume":"206","author":"Zhang","year":"2023","journal-title":"Environ. Exp. Bot."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1080\/01904167.2012.716130","article-title":"Effect of Cadmium on Physiological Responses of Wheat and Corn to Iron Deficiency","volume":"35","author":"Bao","year":"2012","journal-title":"J. Plant Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.plantsci.2011.05.014","article-title":"Comparison of Cadmium-Induced Iron-Deficiency Responses and Genuine Iron-Deficiency Responses in Malus xiaojinensis","volume":"181","author":"Gao","year":"2011","journal-title":"Plant Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1111\/j.1747-0765.2006.00055.x","article-title":"Iron Deficiency Enhances Cadmium Uptake and Translocation Mediated by the Fe2+ Transporters OsIRT1 and OsIRT2 in Rice","volume":"52","author":"Nakanishi","year":"2006","journal-title":"Soil Sci. Plant Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"113410","DOI":"10.1016\/j.envpol.2019.113410","article-title":"Cadmium Absorption and Translocation of Amaranth (Amaranthus mangostanus L.) Affected by Iron Deficiency","volume":"256","author":"Zou","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1104\/pp.116.3.1063","article-title":"The Role of Iron-Deficiency Stress Responses in Stimulating Heavy-Metal Transport in Plants","volume":"116","author":"Cohen","year":"1998","journal-title":"Plant Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1007\/s00128-008-9573-8","article-title":"Iron-Deficiency Induces Cadmium Uptake and Accumulation in Solanum nigrum L.","volume":"82","author":"Bao","year":"2009","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1105\/tpc.001263","article-title":"Expression of the IRT1 Metal Transporter Is Controlled by Metals at the Levels of Transcript and Protein Accumulation","volume":"14","author":"Connolly","year":"2002","journal-title":"Plant Cell"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4843","DOI":"10.1093\/jxb\/err136","article-title":"The OsNRAMP1 Iron Transporter Is Involved in Cd Accumulation in Rice","volume":"62","author":"Takahashi","year":"2011","journal-title":"J. Exp. Bot."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1038\/srep00286","article-title":"Characterizing the Role of Rice NRAMP5 in Manganese, Iron and Cadmium Transport","volume":"2","author":"Ishimaru","year":"2012","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.1105\/tpc.112.096925","article-title":"Nramp5 Is a Major Transporter Responsible for Manganese and Cadmium Uptake in Rice","volume":"24","author":"Sasaki","year":"2012","journal-title":"Plant Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1111\/j.1469-8137.2010.03459.x","article-title":"OsHMA3, a P1B-Type of ATPase Affects Root-to-Shoot Cadmium Translocation in Rice by Mediating Efflux into Vacuoles","volume":"189","author":"Miyadate","year":"2011","journal-title":"New Phytol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1093\/pcp\/pcr166","article-title":"Mutations in Rice (Oryza sativa) Heavy Metal ATPase 2 (OsHMA2) Restrict the Translocation of Zinc and Cadmium","volume":"53","author":"Mori","year":"2012","journal-title":"Plant Cell Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1111\/j.1365-3040.2012.02527.x","article-title":"The OsHMA2 Transporter Is Involved in Root-to-Shoot Translocation of Zn and Cd in Rice","volume":"35","author":"Takahashi","year":"2012","journal-title":"Plant Cell Environ."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"16500","DOI":"10.1073\/pnas.1005396107","article-title":"Gene Limiting Cadmium Accumulation in Rice","volume":"107","author":"Ueno","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"125894","DOI":"10.1016\/j.jhazmat.2021.125894","article-title":"Cell Wall Modification Induced by an Arbuscular Mycorrhizal Fungus Enhanced Cadmium Fixation in Rice Root","volume":"416","author":"Gao","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.jhazmat.2013.09.018","article-title":"Exogenous Auxin Alleviates Cadmium Toxicity in Arabidopsis thaliana by Stimulating Synthesis of Hemicellulose 1 and Increasing the Cadmium Fixation Capacity of Root Cell Walls","volume":"263","author":"Zhu","year":"2013","journal-title":"J. Hazard. Mater."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"111210","DOI":"10.1016\/j.ecoenv.2020.111210","article-title":"The Predominant Role of Pectin in Binding Cd in the Root Cell Wall of a High Cd Accumulating Rice Line (Oryza sativa L.)","volume":"206","author":"Yu","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"112810","DOI":"10.1016\/j.ecoenv.2021.112810","article-title":"The Role of Silicon in Cadmium Alleviation by Rice Root Cell Wall Retention and Vacuole Compartmentalization under Different Durations of Cd Exposure","volume":"226","author":"Wei","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"115665","DOI":"10.1016\/j.envpol.2020.115665","article-title":"Coordination between Root Cell Wall Thickening and Pectin Modification Is Involved in Cadmium Accumulation in Sedum alfredii","volume":"268","author":"Guo","year":"2021","journal-title":"Environ. Pollut."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Yu, R., Jiang, Q., Xv, C., Li, L., Bu, S., and Shi, G. (2019). Comparative Proteomics Analysis of Peanut Roots Reveals Differential Mechanisms of Cadmium Detoxification and Translocation between Two Cultivars Differing in Cadmium Accumulation. BMC Plant Biol., 19.","DOI":"10.1186\/s12870-019-1739-5"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1111\/pce.12203","article-title":"Abscisic Acid Alleviates Iron Deficiency by Promoting Root Iron Reutilization and Transport from Root to Shoot in Arabidopsis","volume":"37","author":"Lei","year":"2014","journal-title":"Plant Cell Environ."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1104\/pp.107.095794","article-title":"Iron Deficiency-Induced Secretion of Phenolics Facilitates the Reutilization of Root Apoplastic Iron in Red Clover","volume":"144","author":"Jin","year":"2007","journal-title":"Plant Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.envexpbot.2018.03.018","article-title":"NH4+ Facilitates Iron Reutilization in the Cell Walls of Rice (Oryza sativa) Roots under Iron-Deficiency Conditions","volume":"151","author":"Zhu","year":"2018","journal-title":"Environ. Exp. Bot."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"10746","DOI":"10.1038\/srep10746","article-title":"Elevation of NO Production Increases Fe Immobilization in the Fe-Deficiency Roots Apoplast by Decreasing Pectin Methylation of Cell Wall","volume":"5","author":"Ye","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s11104-019-04111-z","article-title":"Potassium Ameliorates Iron Deficiency by Facilitating the Remobilization of Iron from Root Cell Walls and Promoting Its Translocation from Roots to Shoots","volume":"440","author":"Ye","year":"2019","journal-title":"Plant Soil"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/s11104-017-3248-3","article-title":"NaCl Alleviates Iron Deficiency through Facilitating Root Cell Wall Iron Reutilization and Its Translocation to the Shoot in Arabidopsis thaliana","volume":"417","author":"Zhu","year":"2017","journal-title":"Plant Soil"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1007\/s00425-012-1652-8","article-title":"Cell Wall Polysaccharides Are Involved in P-Deficiency-Induced Cd Exclusion in Arabidopsis thaliana","volume":"236","author":"Zhu","year":"2012","journal-title":"Planta"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"111605","DOI":"10.1016\/j.plantsci.2023.111605","article-title":"Decrease in Hemicellulose Content and Its Retention of Iron Contributes to Phosphorus Deficiency Alleviated Iron Deficiency in Arabidopsis thaliana","volume":"329","author":"Li","year":"2023","journal-title":"Plant Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1080\/01904167.2014.920392","article-title":"Genotypic Differences in Spectral and Photosynthetic Response of Peanut to Iron Deficiency","volume":"38","author":"Su","year":"2015","journal-title":"J. Plant Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.ecoenv.2014.09.003","article-title":"Relationship between Biomass, Seed Components and Seed Cd Concentration in Various Peanut (Arachis hypogaea L.) Cultivars Grown on Cd-Contaminated Soils","volume":"110","author":"Shi","year":"2014","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/s11104-012-1394-1","article-title":"Peanut as a Potential Crop for Bioenergy Production via Cd-Phytoextraction: A Life-Cycle Pot Experiment","volume":"365","author":"Su","year":"2013","journal-title":"Plant Soil"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s11104-012-1310-8","article-title":"Variation in Cadmium Accumulation and Translocation among Peanut Cultivars as Affected by Iron Deficiency","volume":"363","author":"Su","year":"2013","journal-title":"Plant Soil"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Tan, Z., Li, J., Guan, J., Wang, C., Zhang, Z., and Shi, G. (2023). Genome-Wide Identification and Expression Analysis Reveals Roles of the NRAMP Gene Family in Iron\/Cadmium Interactions in Peanut. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24021713"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Wang, C., Wang, X., Li, J., Guan, J., Tan, Z., Zhang, Z., and Shi, G. (2022). Genome-Wide Identification and Transcript Analysis Reveal Potential Roles of Oligopeptide Transporter Genes in Iron Deficiency Induced Cadmium Accumulation in Peanut. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.894848"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/BF00008069","article-title":"Strategies of Plants for Acquisition of Iron","volume":"165","author":"Marschner","year":"1994","journal-title":"Plant Soil"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.envexpbot.2013.10.001","article-title":"Effects of Iron Deficiency on Subcellular Distribution and Chemical Forms of Cadmium in Peanut Roots in Relation to Its Translocation","volume":"97","author":"Su","year":"2014","journal-title":"Environ. Exp. Bot."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s11104-017-3232-y","article-title":"Iron Supply Prevents Cd Uptake in Arabidopsis by Inhibiting IRT1 Expression and Favoring Competition between Fe and Cd Uptake","volume":"416","author":"He","year":"2017","journal-title":"Plant Soil"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1747-0765.2007.00131.x","article-title":"Cadmium Uptake in Barley Affected by Iron Concentration of the Medium: Role of Phytosiderophores","volume":"53","author":"Kudo","year":"2007","journal-title":"Soil Sci. Plant Nutr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s10725-007-9201-3","article-title":"Iron Nutrition Affects Cadmium Accumulation and Toxicity in Rice Plants","volume":"53","author":"Shao","year":"2007","journal-title":"Plant Growth Regul."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"120875","DOI":"10.1016\/j.indcrop.2025.120875","article-title":"High Iron Supply Enhances Cadmium Accumulation in the Aerial Tissues by Remodeling the Root Cell Walls in Populus cathayana","volume":"228","author":"Yang","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Li, M., Liu, C., Zhang, D., Wang, B., and Ding, S. (2022). The Influence of Iron Application on the Growth and Cadmium Stress Tolerance of Poplar. Forests, 13.","DOI":"10.3390\/f13122023"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Parrotta, L., Guerriero, G., Sergeant, K., Cai, G., and Hausman, J.-F. (2015). Target or Barrier? The Cell Wall of Early- and Later-Diverging Plants vs Cadmium Toxicity: Differences in the Response Mechanisms. Front. Plant Sci., 6.","DOI":"10.3389\/fpls.2015.00133"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1111\/nph.70806","article-title":"Root Cell Wall Plasticity in Iron Homeostasis: An Overlooked Frontier in Plant Nutrition","volume":"249","author":"Kanwar","year":"2026","journal-title":"New Phytol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s11738-010-0581-z","article-title":"The Cell Wall in Plant Cell Response to Trace Metals: Polysaccharide Remodeling and Its Role in Defense Strategy","volume":"33","year":"2011","journal-title":"Acta Physiol. Plant."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1016\/j.tplants.2023.03.007","article-title":"Beyond Iron-Storage Pool: Functions of Plant Apoplastic Iron during Stress","volume":"28","author":"Liu","year":"2023","journal-title":"Trends Plant Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1104\/pp.15.01617","article-title":"Putrescine Alleviates Iron Deficiency via NO-Dependent Reutilization of Root Cell-Wall Fe in Arabidopsis","volume":"170","author":"Zhu","year":"2016","journal-title":"Plant Physiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"112664","DOI":"10.1016\/j.plantsci.2025.112664","article-title":"Cell Wall Polysaccharides Response to Cadmium Stress and Transcriptome Analysis in Tomato (Solanum lycopersicum)","volume":"359","author":"Wang","year":"2025","journal-title":"Plant Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"29705","DOI":"10.1007\/s11356-018-2956-4","article-title":"The Differences of Cell Wall in Roots between Two Contrasting Soybean Cultivars Exposed to Cadmium at Young Seedlings","volume":"25","author":"Wang","year":"2018","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1007\/s11104-019-04313-5","article-title":"Comparative Transcriptome Analysis Reveals Key Genes Responsible for the Homeostasis of Iron and Other Divalent Metals in Peanut Roots under Iron Deficiency","volume":"445","author":"Cao","year":"2019","journal-title":"Plant Soil"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Chen, C., Cao, Q., Jiang, Q., Li, J., Yu, R., and Shi, G. (2019). Comparative Transcriptome Analysis Reveals Gene Network Regulating Cadmium Uptake and Translocation in Peanut Roots under Iron Deficiency. BMC Plant Biol., 19.","DOI":"10.1186\/s12870-019-1654-9"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"5125","DOI":"10.1093\/jxb\/eru272","article-title":"Homogalacturonan-Modifying Enzymes: Structure, Expression, and Roles in Plants","volume":"65","author":"Wattier","year":"2014","journal-title":"J. Exp. Bot."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/S1360-1385(01)02045-3","article-title":"Pectin Methylesterases: Cell Wall Enzymes with Important Roles in Plant Physiology","volume":"6","author":"Micheli","year":"2001","journal-title":"Trends Plant Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1007\/s00425-014-2139-6","article-title":"Identification and Functional Characterization of the Distinct Plant Pectin Esterases PAE8 and PAE9 and Their Deletion Mutants","volume":"240","author":"Hull","year":"2014","journal-title":"Planta"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1016\/j.ygeno.2020.11.006","article-title":"Comprehensive Analysis of Polygalacturonase Genes Offers New Insights into Their Origin and Functional Evolution in Land Plants","volume":"113","author":"Mahmood","year":"2021","journal-title":"Genomics"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"plac003","DOI":"10.1093\/aobpla\/plac003","article-title":"Effects of Polygalacturonase Overexpression on Pectin Distribution in the Elongation Zones of Roots under Aluminium Stress","volume":"14","author":"Nagayama","year":"2022","journal-title":"AoB Plants"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1093\/pcp\/pcab019","article-title":"Structural Alteration of Rice Pectin Affects Cell Wall Mechanical Strength and Pathogenicity of the Rice Blast Fungus Under Weak Light Conditions","volume":"62","author":"Ohara","year":"2021","journal-title":"Plant Cell Physiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/BF03323431","article-title":"Plant \u03b2-Galactosidases: Physiological Significance and Recent Advances in Technological Applications","volume":"19","author":"Dwevedi","year":"2010","journal-title":"J. Plant Biochem. Biotechnol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/0043-1354(86)90198-3","article-title":"Inhibition of the Activities of \u03b2-Galactosidase and Dehydrogenases of Activated Sludge by Heavy Metals","volume":"20","year":"1986","journal-title":"Water Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.envexpbot.2013.12.021","article-title":"Overexpression of Populus euphratica Xyloglucan Endotransglucosylase\/Hydrolase Gene Confers Enhanced Cadmium Tolerance by the Restriction of Root Cadmium Uptake in Transgenic Tobacco","volume":"100","author":"Han","year":"2014","journal-title":"Environ. Exp. Bot."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"4731","DOI":"10.1105\/tpc.112.106039","article-title":"XTH31, Encoding an in Vitro XEH\/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in Arabidopsis","volume":"24","author":"Zhu","year":"2012","journal-title":"Plant Cell"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1007\/s11105-020-01233-y","article-title":"Identification of Genes Involved in Root Growth Inhibition Under Lead Stress by Transcriptome Profiling in Arabidopsis","volume":"39","author":"Zheng","year":"2021","journal-title":"Plant Mol. Biol. Rep."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Liu, Q., Luo, L., Wang, X., Shen, Z., and Zheng, L. (2017). Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18020209"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1093\/jxb\/44.4.763","article-title":"Spatial and Temporal Aspects of Growth in the Primary Root of Cotton Seedlings: Effects of NaCl and CaCl2","volume":"44","author":"Zhong","year":"1993","journal-title":"J. Exp. Bot."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1104\/pp.111.172221","article-title":"Cell Wall Hemicellulose Contributes Significantly to Aluminum Adsorption and Root Growth in Arabidopsis","volume":"155","author":"Yang","year":"2011","journal-title":"Plant Physiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"106250","DOI":"10.1016\/j.envexpbot.2025.106250","article-title":"Integrative Transcriptomic and Metabolomic Analyses Reveal Crucial Roles of Phenylpropanoid-Derived Coumarin Biosynthesis in the Responses of Peanut to Iron Deficiency","volume":"238","author":"Ma","year":"2025","journal-title":"Environ. Exp. Bot."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2641\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:28:55Z","timestamp":1788150535000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2641"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,28]]},"references-count":72,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["plants15172641"],"URL":"https:\/\/doi.org\/10.3390\/plants15172641","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,28]]}}}