{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:18:00Z","timestamp":1788430680132,"version":"build-2803163510"},"reference-count":64,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32260072"],"award-info":[{"award-number":["32260072"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32260072"],"award-info":[{"award-number":["32260072"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Graduate Student Research and Innovation Projects at Xinjiang University","award":["XJDX2025YJS060"],"award-info":[{"award-number":["XJDX2025YJS060"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Halostachys caspica is a salt-tolerant shrub widely distributed on saline-alkali soils in Xinjiang, but research on its salt-tolerance mechanisms has been limited by the lack of reliable regeneration and transformation protocols. We optimized seed germination on 1\/2 MS medium with 45 g\/L sucrose, significantly raising rates from 8.7% to 70.3% and providing adequate explant material. For regeneration, we developed two complementary protocols: a conventional route using seedling-derived assimilation branches, with optimal adventitious bud induction on medium containing 100 mM NaCl and 1 mg\/L 6-BA, followed by transfer to proliferation medium (100 mM NaCl, 0.8 mg\/L 6-BA, 0.5 mg\/L IBA) that resulted in simultaneous proliferation, elongation, and rooting; and an accelerated protocol using regenerated plantlet assimilation branches, which produced complete plantlets within 35 days on medium with 1 mg\/L IBA and 100 mM NaCl, establishing an effective rapid propagation system. Using these explants, we optimized Agrobacterium-mediated transformation parameters, achieving over 90% transient transformation efficiency. Together, this regeneration and transformation platform will facilitate functional dissection of stress-responsive genes and regulatory networks, while the rapid propagation protocol provides reliable planting material and may serve as a reference for other woody plants and halophytes lacking established regeneration systems.<\/jats:p>","DOI":"10.3390\/plants15172645","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:33:52Z","timestamp":1787942032000},"page":"2645","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Efficient Regeneration and Transient Transformation System for the Desert Halophyte Halostachys caspica"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2201-3042","authenticated-orcid":false,"given":"Shufang","family":"Tan","sequence":"first","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Li","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Yi","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyanzi","family":"Zhan","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adili","family":"Tulaerbieke","sequence":"additional","affiliation":[{"name":"National Comprehensive Monitoring Station for Ecological Quality, Xinjiang Junggar Station, Xinjiang Institute of Ecology and Environment Sciences, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s11240-025-03129-9","article-title":"In vitro propagation of Erben: Towards the conservation of a rare and endemic halophyte","volume":"162","author":"Custodio","year":"2025","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4279","DOI":"10.1038\/s41467-024-48595-5","article-title":"SOS1 tonoplast neo-localization and the RGG protein SALTY are important in the extreme salinity tolerance of Salicornia bigelovii","volume":"15","author":"Salazar","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2558","DOI":"10.1111\/pbi.14368","article-title":"The genome sequence provides insights into the salt-tolerance mechanisms of exo-recretohalophytes","volume":"22","author":"Chen","year":"2024","journal-title":"Plant Biotechnol. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1007\/s00344-023-11210-w","article-title":"Efficient In Vitro Platform for Multiplication, Acclimatization, and Deliver of High-NaCl-Tolerant Clones of the Halophyte","volume":"43","author":"Piqueras","year":"2024","journal-title":"J. Plant Growth Regul."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Cust\u00f3dio, L., Charles, G., Magn\u00e9, C., Barba-Esp\u00edn, G., Piqueras, A., Hern\u00e1ndez, J.A., Ben Hamed, K., Casta\u00f1eda-Loaiza, V., Fernandes, E., and Rodrigues, M.J. (2022). Application of In Vitro Plant Tissue Culture Techniques to Halophyte Species: A Review. Plants, 12.","DOI":"10.3390\/plants12010126"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Long, Y., Yang, Y., Pan, G.T., and Shen, Y.U. (2022). New Insights into Tissue Culture Plant-Regeneration Mechanisms. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.926752"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"123","DOI":"10.31421\/IJHS\/20\/3-4\/1147","article-title":"Development of in vitro propagation system for Atriplex halimus L.","volume":"20","author":"Aldahhak","year":"2014","journal-title":"Int. J. Hortic. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2034","DOI":"10.1111\/pbi.70028","article-title":"Plant genetic transformation: Achievements, current status and future prospects","volume":"23","author":"Wang","year":"2025","journal-title":"Plant Biotechnol. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1016\/j.biotechadv.2015.03.012","article-title":"Transient plant transformation mediated by Agrobacterium tumefaciens: Principles, methods and applications","volume":"33","author":"Krenek","year":"2015","journal-title":"Biotechnol. Adv."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Mo, R.L., Zhang, N., Qiu, C.Y., Huang, S., Wei, W., Zhang, C.H., Liu, D., and Lin, Q. (2024). Refinement and Enhancement of Agrobacterium-Mediated Transient Transformation for Functional Gene Examination in Mulberry (Morus L.). Genes, 15.","DOI":"10.3390\/genes15101277"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Basak, S., Parajulee, D., Dhir, S., Sangra, A., and Dhir, S.K. (2024). Improved Protocol for Efficient Agrobacterium-Mediated Transient Gene Expression in Medicago sativa L.. Plants, 13.","DOI":"10.20944\/preprints202408.1874.v1"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Mohammad, T., Ghogare, R., Morton, L.B., Dhingra, A., Potlakayala, S., Rudrabhatla, S., and Dhir, S.K. (2024). Evaluation of Parameters Affecting Agrobacterium-Mediated Transient Gene Expression in Industrial Hemp (Cannabis sativa L.). Plants, 13.","DOI":"10.3390\/plants13050664"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zhao, H., Jia, Y., Cao, Y., and Wang, Y. (2020). Improving T-DNA Transfer to Tamarix hispida by Adding Chemical Compounds During Agrobacterium tumefaciens Culture. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.501358"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/j.hpj.2024.06.001","article-title":"An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops","volume":"10","author":"Yu","year":"2024","journal-title":"Hortic. Plant J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1007\/s00299-009-0717-z","article-title":"Optimization of conditions for transient Agrobacterium-mediated gene expression assays in Arabidopsis","volume":"28","author":"Kim","year":"2009","journal-title":"Plant Cell Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1093\/jxb\/erj108","article-title":"World salinization with emphasis on Australia","volume":"57","author":"Rengasamy","year":"2006","journal-title":"J. Exp. Bot."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s11103-024-01536-8","article-title":"Heterologous expression of pathogenesis-related protein 10 increases salt and drought resistance in transgenic","volume":"115","author":"Cao","year":"2025","journal-title":"Plant Mol. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"13199","DOI":"10.1038\/s41598-024-61737-5","article-title":"Effect of salt-alkali stress on seed germination of the halophyte Halostachys caspica","volume":"14","author":"Zhang","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"107763","DOI":"10.1016\/j.plaphy.2023.107763","article-title":"A novel metallothionein gene HcMT from halophyte shrub Halostachys caspica respond to cadmium and sodium stress","volume":"201","author":"Yan","year":"2023","journal-title":"Plant Physiol. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Yin, F.L., Zeng, Y.L., Ji, J.Y., Wang, P.J., Zhang, Y.F., and Li, W.H. (2021). The Halophyte Halostachys caspica AP2\/ERF Transcription Factor HcTOE3 Positively Regulates Freezing Tolerance in Arabidopsis. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.638788"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"110344","DOI":"10.1016\/j.plaphy.2025.110344","article-title":"RNase activity of Halostachys caspica pathogenesis-related protein 10 contributes to enhanced biotic tolerance in transgenic Arabidopsis","volume":"229","author":"Liu","year":"2025","journal-title":"Plant Physiol. Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1007\/s00468-021-02246-0","article-title":"Potassium transporter HcKUP12 from Halostachys caspica improved transgenic Arabidopsis salt tolerance through maintaining potassium homeostasis","volume":"36","author":"Zhang","year":"2022","journal-title":"Trees"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.plaphy.2020.03.020","article-title":"Overexpression of HcSCL13, a Halostachys caspica GRAS transcription factor, enhances plant growth and salt stress tolerance in transgenic Arabidopsis","volume":"151","author":"Zhang","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_24","first-page":"14","article-title":"In vitro regeneration and Agrobacterium-mediated genetic transformation of Caragana korshinskii","volume":"3","author":"Liu","year":"2023","journal-title":"For. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"120328","DOI":"10.1016\/j.indcrop.2024.120328","article-title":"Establishment of in vitro efficient regeneration system for leaves and hypocotyls of Toona sinensis, a multifunctional woody plants","volume":"224","author":"Li","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1093\/jxb\/ery247","article-title":"DA-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism","volume":"70","author":"Zhou","year":"2019","journal-title":"J. Exp. Bot."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zhang, M., Li, B., Wan, Z.L., Chen, X.F., Liu, C., Liu, C.J., and Zhou, Y.F. (2022). Exogenous Spermidine Promotes Germination of Aged Sorghum Seeds by Mediating Sugar Metabolism. Plants, 11.","DOI":"10.3390\/plants11212853"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Lv, T., Zhong, L., Li, J., Chen, C., Xian, B., Zhou, T., Ren, C., Chen, J., Pei, J., and Yan, J. (2025). Exogenous Sucrose Improves the Vigor of Aged Safflower Seeds by Mediating Fatty Acid Metabolism and Glycometabolism. Plants, 14.","DOI":"10.3390\/plants14152301"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Alexopoulos, A.A., Mavrommati, E., Kartsonas, E., and Petropoulos, S.A. (2022). Effect of Temperature and Sucrose on In Vitro Seed Germination and Bulblet Production of Pancratium maritimum L.. Agronomy, 12.","DOI":"10.3390\/agronomy12112786"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1080\/14620316.2002.11511489","article-title":"Enhanced growth of micropropagated bulblets of Lilium sp. inoculated with arbuscular mycorrhizal fungi at different P fertility levels in an alfisol","volume":"77","author":"Varshney","year":"2002","journal-title":"J. Hortic. Sci. Biotechol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1007\/s002990050636","article-title":"Callus induction, somatic embryogenesis and organogenesis in Narcissus confusus: Correlation between the state of differentiation and the content of galanthamine and related alkaloids","volume":"18","author":"Viladomat","year":"1999","journal-title":"Plant Cell Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"012141","DOI":"10.1088\/1755-1315\/794\/1\/012141","article-title":"The Effect of Sucrose on Growth and Morphology of Rodent Tuber (Typhonium flagelliforme) Plantlets in Minimal Growth Preservation in vitro","volume":"794","author":"Sianipar","year":"2021","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"582","DOI":"10.2225\/vol10-issue4-fulltext-7","article-title":"Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulators, sucrose concentrations, harvesting season and cold treatments","volume":"10","author":"Azadi","year":"2007","journal-title":"Electron. J. Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"134069","DOI":"10.1016\/j.foodchem.2022.134069","article-title":"Sucrose-induced abiotic stress improves the phytochemical profiles and bioactivities of mung bean sprouts","volume":"400","author":"Yu","year":"2023","journal-title":"Food Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s11240-019-01730-3","article-title":"Timely removal of exogenous cytokinin and the prevention of auxin transport from the shoot to the root affect the regeneration potential of Arabidopsis roots","volume":"140","author":"Bernula","year":"2020","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"120698","DOI":"10.1016\/j.indcrop.2025.120698","article-title":"Establishment of an efficient regeneration system for Lycium barbarum","volume":"226","author":"Zhang","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s11240-025-03026-1","article-title":"Regeneration of in vitro plants through direct and indirect organogenesis from Dracocephalum rupestre leaf explants","volume":"161","author":"Wang","year":"2025","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_38","first-page":"7","article-title":"In vitro propagation, shoot regeneration, callus induction, and suspension from lamina explants of Sorbus caloneura","volume":"3","author":"Guo","year":"2023","journal-title":"For. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"436","DOI":"10.21273\/HORTSCI14584-19","article-title":"In Vitro Propagation and NaCl Tolerance of the Multipurpose Medicinal Halophyte","volume":"55","author":"Martini","year":"2020","journal-title":"Hortscience"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.indcrop.2011.06.024","article-title":"NaCl plays a key role for in vitro micropropagation of Salicornia brachiata, an extreme halophyte","volume":"35","author":"Joshi","year":"2012","journal-title":"Ind. Crops Prod."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s11240-008-9406-9","article-title":"Micropropagation and salt tolerance of in vitro grown Crithmum maritimum L.","volume":"94","author":"Grigoriadou","year":"2008","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"13639","DOI":"10.1038\/srep13639","article-title":"Contribution and distribution of inorganic ions and organic compounds to the osmotic adjustment in response to salt stress","volume":"5","author":"Zeng","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/s13007-020-00599-0","article-title":"An efficient in vitro regeneration system from different wild apple (Malus sieversii) explants","volume":"16","author":"Zhang","year":"2020","journal-title":"Plant Methods"},{"key":"ref_44","first-page":"1","article-title":"Effect of 6-BA on the Plant Regeneration via Organogenesis from Cotyledonary Node of Cowpea (Vigna unguiculata L. Walp)","volume":"5","author":"Tie","year":"2013","journal-title":"J. Agric. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Liu, L., Sun, M., Qi, X., Zhang, J., Jiang, M., Cai, H., Yang, M., and Wang, J. (2025). Establishment of a leaf regeneration system and its molecular basis in Poplar 741. BMC Plant Biol., 25.","DOI":"10.1186\/s12870-025-07091-8"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Mann, A., Lata, C., Kumar, N., Kumar, A., Kumar, A., and Sheoran, P. (2023). Halophytes as new model plant species for salt tolerance strategies. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1137211"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Lao, X.A., Jin, P., Yang, R., Liang, Y., Zhang, D., Zeng, Y., and Li, X.S. (2024). Establishment of Agrobacterium-Mediated Transient Transformation System in Desert Legume Eremosparton songoricum (Litv.) Vass. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252211934"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Dias, S.L., Rizzo, P., D\u2019Auria, J.C., and Kochevenko, A. (2025). Efficient Agrobacterium-Mediated Methods for Transient and Stable Transformation in Common and Tartary Buckwheat. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26094425"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"17071","DOI":"10.1038\/s41598-018-34949-9","article-title":"Stable pH Suppresses Defense Signaling and is the Key to Enhance Agrobacterium-Mediated Transient Expression in Arabidopsis Seedlings","volume":"8","author":"Wang","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"121350","DOI":"10.1016\/j.indcrop.2025.121350","article-title":"Revolutionizing poplar biotechnology: Genetic transformation and CRISPR\/Cas strategies","volume":"233","author":"Ming","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1007\/s00299-010-0960-3","article-title":"Potassium chloride and rare earth elements improve plant growth and increase the frequency of the Agrobacterium tumefaciens-mediated plant transformation","volume":"30","author":"Boyko","year":"2011","journal-title":"Plant Cell Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/s41596-022-00749-9","article-title":"Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana","volume":"18","author":"Cody","year":"2023","journal-title":"Nat. Protoc."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Sivanandhan, G., Bae, S., Sung, C., Choi, S.R., Lee, G.J., and Lim, Y.P. (2021). Optimization of protoplast isolation from leaf mesophylls of Chinese cabbage (Brassica rapa ssp. pekinensis) and subsequent transfection with a binary vector. Plants, 10.","DOI":"10.3390\/plants10122636"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1073\/pnas.0406241102","article-title":"Positive charge is an important feature of the C-terminal transport signal of the VirB\/D4-translocated proteins of Agrobacterium","volume":"102","author":"Vergunst","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Sivanandhan, G., Dev, G.K., Theboral, J., Selvaraj, N., Ganapathi, A., and Manickavasagam, M. (2015). Sonication, Vacuum Infiltration and Thiol Compounds Enhance the Agrobacterium-Mediated Transformation Frequency of Withania somnifera (L.) Dunal. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0124693"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Guan, S.X., Kang, X.N., Ge, J.Y., Fei, R.W., Duan, S.Y., and Sun, X.M. (2022). An efficient Agrobacterium-mediated transient transformation system and its application in gene function elucidation in Paeonia lactiflora Pall. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.999433"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1111\/pbi.12988","article-title":"Multi-omics analyses reveal epigenomics basis for cotton somatic embryogenesis through successive regeneration acclimation process","volume":"17","author":"Li","year":"2019","journal-title":"Plant Biotechnol. J."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1007\/s11105-013-0683-z","article-title":"A Transient Transformation System for the Functional Characterization of Genes Involved in Stress Response","volume":"32","author":"Ji","year":"2014","journal-title":"Plant Mol. Biol. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","article-title":"A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures","volume":"15","author":"Murashige","year":"1962","journal-title":"Physiol. Plant."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Rizwan, H.M., Yang, Q., Yousef, A.F., Zhang, X.X., Sharif, Y., Kaijie, J., Shi, M., Li, H., Munir, N., and Yang, X.L. (2021). Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis). Plants, 10.","DOI":"10.3390\/plants10112459"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.hpj.2020.03.004","article-title":"Reliable and Efficient Agrobacterium tumefaciens-Mediated Genetic Transformation of Dianthus spiculifolius","volume":"6","author":"Wang","year":"2020","journal-title":"Hortic. Plant J."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s11240-009-9567-1","article-title":"Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus","volume":"98","author":"Dutt","year":"2009","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11627-013-9544-6","article-title":"Development of an efficient regeneration and Agrobacterium-mediated transformation system in crab apple (Malus micromalus) using cotyledons as explants","volume":"50","author":"Dai","year":"2014","journal-title":"In Vitro Cell. Dev. Biol. Plant"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"30363","DOI":"10.1038\/srep30363","article-title":"Selection of suitable reference genes for quantitative RT-PCR normalization in the halophyte under salt and drought stress","volume":"6","author":"Zhang","year":"2016","journal-title":"Sci. Rep."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2645\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:46:47Z","timestamp":1788428807000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2645"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,28]]},"references-count":64,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["plants15172645"],"URL":"https:\/\/doi.org\/10.3390\/plants15172645","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,28]]}}}