{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T03:15:50Z","timestamp":1788146150055,"version":"build-2803163510"},"reference-count":92,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education","doi-asserted-by":"publisher","award":["SUBB.WLR.19.071 RiO"],"award-info":[{"award-number":["SUBB.WLR.19.071 RiO"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]},{"award":["SUBB.WLR.19.071 RiO"],"award-info":[{"award-number":["SUBB.WLR.19.071 RiO"]}],"id":[{"id":"https:\/\/ror.org\/05dwvd537","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Osmophores, which constitute scent-releasing glands in flowers, have so far been insufficiently characterised. This study is the first to present the structure of scent glands exhibiting features consistent with those of osmophores in two Myosotis species (Boraginaceae). Petal tissues of Myosotis scorpioides and M. sylvatica were analysed using light microscopy, scanning electron microscopy, and transmission electron microscopy. Histochemical tests were performed to detect fragrance-producing tissues (neutral red), phenols (Toluidine blue O), lipids (Sudan III, Sudan Red B), and starch (Lugol solution). The study has shown that the epidermal cells within the yellow nectar guides (faucal scales) were differentiated, as they comprised palisade-like cells, papillae, and unicellular trichomes. The petal appendages forming white ridges on the petals were covered with papillae. Histochemical assays revealed the presence of scent-producing epidermal cells (palisade-like, papillae, unicellular trichomes), lipids (which is typical of scent glands) and starch during the pre-secretory phase. The ultrastructure of the examined cells exhibited dense cytoplasm, large nuclei, and numerous mitochondria and plastids. Such organelle composition is associated with secretory activity, which is further supported by the presence of numerous secretory vesicles located in the vicinity of the plasmalemma, releasing their contents into the periplasmic space. The histochemical analyses and the observations of the anatomical structure and ultrastructure of the cells indicated that both the faucal scales and the petal appendages exhibit features of osmophores. These findings contribute to expanding the extant knowledge on the structure of scent glands with traits typical of osmophores and the characteristics of the Boraginaceae family.<\/jats:p>","DOI":"10.3390\/plants15172658","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T02:08:58Z","timestamp":1788142138000},"page":"2658","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Do Nectar Guides in Flowers of Two Myosotis L. Species (Boraginaceae) Serve as Osmophores?"],"prefix":"10.3390","volume":"15","author":[{"given":"El\u017cbieta","family":"Weryszko-Chmielewska","sequence":"first","affiliation":[{"name":"Department of Botany and Plant Physiology, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneta","family":"Sulborska-R\u00f3\u017cycka","sequence":"additional","affiliation":[{"name":"Department of Botany and Plant Physiology, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9930-0633","authenticated-orcid":false,"given":"Marta","family":"Dmitruk","sequence":"additional","affiliation":[{"name":"Department of Botany and Plant Physiology, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"107093","DOI":"10.1016\/j.isci.2023.107093","article-title":"Honesty, reliability, and information content of floral signals","volume":"26","author":"Reuvers","year":"2023","journal-title":"iScience"},{"key":"ref_2","unstructured":"Wasser, S.P. (1999). The functional ecology of floral guides in relation to insects behaviour and visio. Evolutionary Theory and Processes: Modern Perspectives, Springer."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Leonard, A.S., Brent, J., Papaj, D.R., and Dornhaus, A. (2013). Floral nectar guide patterns discourage nectar robbing by bumble bees floral nectar guide patterns discourage nectar robbing by bumble bees. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0055914"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"103474","DOI":"10.1016\/j.micron.2023.103474","article-title":"Nectar guides and floral nectary in Lamium album subsp. album L.: Structure and histochemistry in light, fluorescence, and electron microscopy","volume":"171","author":"Konarska","year":"2023","journal-title":"Micron"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5","DOI":"10.55671\/2767-1380.1122","article-title":"Floral cues and foraging preferences in various species of wild bumblebees","volume":"10","author":"Dahl","year":"2025","journal-title":"Int. J. Ecopsychology (IJE)"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"101086","DOI":"10.1016\/j.cois.2023.101086","article-title":"Multimodal floral recognition by bumblebees","volume":"59","author":"Rands","year":"2023","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s11829-007-9014-3","article-title":"Morphology and development of floral features recognised by pollinators","volume":"1","author":"Whitney","year":"2007","journal-title":"Arthropod-Plant Interact."},{"key":"ref_8","unstructured":"Pua, E.C., and Davey, M.R. (2010). Biosynthesis and regulation of flower scent. Plant Developmental Biology\u2013Biotechnological Perspectives, Springer-Verlag. [2nd ed.]. Available online: https:\/\/api.semanticscholar.org\/CorpusID:92986425."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"11050","DOI":"10.1038\/s41598-017-10975-x","article-title":"The network of plants volatile organic compounds","volume":"7","author":"Vivaldo","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Jin, W., Yang, Z., Xu, K., Liu, Q., Luo, Q., Li, L., and Xiang, X.A. (2025). Comprehensive review of plant volatile terpenoids, elucidating interactions with surroundings, systematic synthesis, regulation, and targeted engineering production. Biology, 14.","DOI":"10.3390\/biology14050466"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1111\/j.1365-2435.2010.01744.x","article-title":"Host-plant finding and recognition by visual and olfactory floral cues in an oligolectic bee","volume":"24","author":"Burger","year":"2010","journal-title":"Funct. Ecol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Faegri, K., and Pijl, L.V.D. (1979). The Principles of Pollination Ecology, Pergamon Press. [Revised ed.].","DOI":"10.1016\/B978-0-08-023160-0.50020-7"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1007\/BF00297747","article-title":"Duftmale an Bl\u00fcten","volume":"36","author":"Lex","year":"1954","journal-title":"Zeitschr. Vergl. Physiol."},{"key":"ref_14","first-page":"143","article-title":"Differences in fragrance chemistry between flower parts of Rosa rugosa Thunb. (Rosaceae)","volume":"39","author":"Dobson","year":"1990","journal-title":"Isr. J. Plant Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1007\/s10886-006-9044-9","article-title":"Analysis of the volatiles emitted by whole flowers and isolated flower organs of the carob tree using HS-SPME-GC\/MS","volume":"32","author":"Serra","year":"2006","journal-title":"J. Chem. Ecol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.3732\/ajb.1400368","article-title":"Annular floral nectary with oil-producing trichomes in Salvia farinacea (Lamiaceae): Anatomy, histochemistry, ultrastructure, and significance","volume":"101","author":"Zhang","year":"2014","journal-title":"Am. J. Bot."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Konarska, A., Weryszko-Chmielewska, E., Dmitruk, M., Sulborska-R\u00f3\u017cycka, A., and Piotrowska-Weryszko, K. (2022). Does the floral nectary in Dracocephalum moldavica L. produce nectar and essential oil? Structure and histochemistry of the nectary. Biology, 11.","DOI":"10.3390\/biology11111650"},{"key":"ref_18","unstructured":"Vogel, S. (1963). The Role of Scent Glands in Pollination. On the Structure and Function of Osmophores, Smithsonian Institution Libraries."},{"key":"ref_19","unstructured":"M\u00e9rillon, J.M., and Ramawat, K.G. (2020). Diversity of floral glands and their secretions in pollinator attraction. Co-Evolution of Secondary Metabolites, Springer."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Dudareva, N., and Pichersky, E. (2006). Localization of the synthesis and emission of scent compounds within the flower. Biology of Floral Scent, CRC Press.","DOI":"10.1201\/9781420004007"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s00606-010-0294-1","article-title":"Morphological and histological characterization of the osmophores and nectaries of four species of Acianthera (Orchidaceae: Pleurothallidinae)","volume":"286","author":"Melo","year":"2010","journal-title":"Plant Syst. Evol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"11","DOI":"10.5586\/aa.2012.054","article-title":"Comparative structure of the osmophores in the flower of Stanhopea graveolens Lindley and Cycnoches chlorochilon Klotzsch (Orchidaceae)","volume":"65","year":"2012","journal-title":"Acta Agrobot."},{"key":"ref_23","unstructured":"Dressler, R.L. (1993). Phylogeny and Classification of the Orchid Family, Dioscorides Press."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1007\/s00709-021-01624-2","article-title":"Floral glands in myophilous and sapromyophilous species of Pleurothallidinae (Epidendroideae, Orchidaceae) osmophores, nectaries, and a unique sticky gland","volume":"258","author":"Davis","year":"2021","journal-title":"Protoplasma"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"152851","DOI":"10.1016\/j.flora.2025.152851","article-title":"Osmophores as key players in pollination of Caladium bicolor (Araceae): A structural approach","volume":"332","author":"Paiva","year":"2025","journal-title":"Flora"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"355","DOI":"10.2307\/1541248","article-title":"Orchid floral fragrances and male euglossine bees: Methods and ad-vances in the last sesquidecade","volume":"164","author":"Williams","year":"1983","journal-title":"Biol. Bull."},{"key":"ref_27","first-page":"ply062","article-title":"Osmophores and floral fragrance in Anacardium humile and Mangifera indica (Anacardiaceae): An overlooked secretory structure in Sapindales","volume":"10","author":"Bachelier","year":"2018","journal-title":"AoB Plants"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"151799","DOI":"10.1016\/j.flora.2021.151799","article-title":"First report of osmophores and wet stigma in Podostemaceae with notes on floral biology and pollination of Weddellina squamulosa Tul","volume":"278","author":"Koschnitzke","year":"2021","journal-title":"Flora"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1007\/s00114-026-02077-4","article-title":"Corolla traits and their osmophores in three neotropical Malpighiaceae: The putative impacts on plant-pollinator interactions","volume":"113","author":"Possobom","year":"2026","journal-title":"Sci. Nat."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Francisco, A., and Ascens\u00e3o, L. (2024). Osmophore structure and labellum micromorphology in Ophrys speculum (Orchidaceae): New interpretations of floral features and implications for a specific sexually deceptive pollination interaction. Plants, 13.","DOI":"10.3390\/plants13101413"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1007\/s00114-023-01873-6","article-title":"Osmophores and petal surface traits in Bignonieae species","volume":"110","author":"Macedo","year":"2023","journal-title":"Sci. Nat."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"523","DOI":"10.12705\/653.6","article-title":"The borage family (Boraginaceae s.str.): A revised infrafamilial classification based on new phylogenetic evidence, with emphasis on the placement of some enigmatic genera","volume":"65","author":"Luebert","year":"2016","journal-title":"Taxon"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"502","DOI":"10.12705\/653.5","article-title":"Familial classification of the Boraginales","volume":"65","author":"Luebert","year":"2016","journal-title":"Taxon"},{"key":"ref_34","unstructured":"(2026, June 10). POWO. \u201cBoraginaceae Juss,\u201d Plants of the World Online. Kew Science. Available online: https:\/\/powo.science.kew.org\/taxon\/urn:lsid:ipni.org:names:30000135-2."},{"key":"ref_35","unstructured":"POWO (2026, June 10). \u201cMyosotis L.,\u201d Plants of the World Online. Kew Science\n          . Available online: https:\/\/powo.science.kew.org\/taxon\/urn:lsid:ipni.org:names:30010296-2."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"719","DOI":"10.2307\/4135544","article-title":"Boraginaceae cymes are exclusively scorpioid and not helicoid","volume":"52","author":"Buys","year":"2003","journal-title":"Taxon"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Kadereit, J., and Bittrich, V. (2016). Boraginaceae. Flowering Plants. Eudicots. The Families and Genera of Vascular Plants, Springer.","DOI":"10.1007\/978-3-319-28534-4"},{"key":"ref_38","unstructured":"Proctor, M., Yeo, P., and Lack, A. (1996). The Natural History of Pollination, HarperCollins Publishers."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1093\/botlinnean\/boz112","article-title":"Towards an integrative understanding of stamen\u2013corolla tube modifications and floral architecture in Boraginaceae s.s. (Boraginales)","volume":"193","author":"Jeiter","year":"2020","journal-title":"Bot. J. Linn. Soc."},{"key":"ref_40","first-page":"43","article-title":"Morphology and anatomy of floral nectary and corolla outgrowths of Myosotis sylvatica Hoffm. (Boraginaceae)","volume":"45","year":"2003","journal-title":"Acta Biol. Cracoviensia Ser. Bot."},{"key":"ref_41","first-page":"196668","article-title":"Plasmodial tapetum, orbicules, and ultrastructure of Myosotis scorpioides L. (Boraginaceae) pollen grains","volume":"94","year":"2025","journal-title":"Acta Soc. Bot. Pol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Nicolson, S.W., Nepi, M., and Pacini, E. (2007). A systematic survey of floral nectaries. Nectaries and Nectar, Springer.","DOI":"10.1007\/978-1-4020-5937-7"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"103773","DOI":"10.1016\/j.micron.2024.103773","article-title":"Myosotis scorpioides L. (Boraginaceae) floral nectary: Micromorphology, fluorescency and ultrastructure","volume":"190","year":"2025","journal-title":"Micron"},{"key":"ref_44","unstructured":"Rutkowski, L. (2008). Klucz do Oznaczania ro\u015blin Naczyniowych Polski Ni\u017cowej, PWN."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/354227a0","article-title":"Floral colour changes as cues for pollinators","volume":"354","author":"Weiss","year":"1991","journal-title":"Nature"},{"key":"ref_46","unstructured":"Lipi\u0144ski, M. (2010). Po\u017cytki Pszczele. Zapylanie i Miododajno\u015b\u0107 Ro\u015blin, PWRiL."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1007\/s00359-016-1107-y","article-title":"Flower detection and acuity of the Australian native stingless bee Tetragonula carbonaria","volume":"202","author":"Dyer","year":"2016","journal-title":"Sm. J. Comp. Physiol. A"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Lunau, K., Konzmann, S., Bossems, J., and Harpke, D.A. (2016). Matter of contrast: Yellow flower colour constrains style length in Crocus species. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0154728"},{"key":"ref_49","unstructured":"Christensen, K.I., and Hansen, H.V. (1998). SEM-Studies of Epidermal Patterns of Petals in the Angiosperms, Forlag uden navn. Ser. Opera Botanica 135."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"525","DOI":"10.4161\/psb.2.6.4659","article-title":"Production and emission of volatile compounds by petal cells","volume":"6","author":"Baudino","year":"2007","journal-title":"Plant Signal. Behav."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1007\/s00425-007-0531-1","article-title":"Both the adaxial and abaxial epidermis layers of the rose petal emit volatile scent compounds","volume":"226","author":"Bergognoux","year":"2007","journal-title":"Planta"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1093\/aob\/mcr065","article-title":"Why do so many petals have conical epidermal cells?","volume":"108","author":"Whitney","year":"2011","journal-title":"Ann. Bot."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Ba\u015fer, K.H.C., and Buchbauer, G. (2020). Functions of essential oils and natural volatiles in plant-insect interactions. Handbook of Essential Oils: Science, Technology and Applications, CRC Press, Taylor & Francis.","DOI":"10.1201\/9781351246460"},{"key":"ref_54","unstructured":"Dudareva, N., and Pichersky, E. (2020). Behavioral responses to floral scent: Experimental manipulations and multimodal plant-pollinator communication. Biology of Plant Volatiles, CRC Press, Taylor & Francis."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.copbio.2008.02.011","article-title":"Metabolic engineering of plant volatiles","volume":"19","author":"Dudareva","year":"2008","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/j.sajb.2010.03.003","article-title":"Sites of synthesis, biochemistry and functional role of plant volatiles","volume":"76","author":"Maffei","year":"2010","journal-title":"S. Afr. J. Bot."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/aob\/mcv024","article-title":"A theoretical approach to the relationship between wettability and surface microstructures of epidermal cells and structured cuticles of flower petals","volume":"115","author":"Taneda","year":"2015","journal-title":"Ann. Bot."},{"key":"ref_58","first-page":"533","article-title":"Unveiling the osmophores of Philodendron adamantinum (Araceae) as a means to understanding interactions with pollinators","volume":"119","author":"Schlindwein","year":"2017","journal-title":"Ann. Bot."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1002\/j.1537-2197.1991.tb12585.x","article-title":"Osmophores, floral features, and systematics of Stanhopea (Orchidaceae)","volume":"78","author":"Curry","year":"1991","journal-title":"Am. J. Bot."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"plu090","DOI":"10.1093\/aobpla\/plu090","article-title":"Structural and biochemical characteristics of citrus flowers associated with defense against a fungal pathogen","volume":"7","author":"Marques","year":"2015","journal-title":"AoB Plants"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"10084","DOI":"10.1073\/pnas.92.22.10084","article-title":"Pathway of terpene excretion by the appendix of Sauromatum guttatum","volume":"92","author":"Skubatz","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1002\/j.1537-2197.1987.tb08748.x","article-title":"Initiation of terpenoid synthesis in osmophores of Stanhopea anfracta (Orchidaceae): A cytochemical study","volume":"74","author":"Curry","year":"1987","journal-title":"Am. J. Bot."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.flora.2009.01.002","article-title":"Can a stench be beautiful? Osmophores in stem-succulent stapeliads (Apocynaceae-Asclepiadoideae-Ceropegieae-Stapeliinae)","volume":"205","author":"Szymczak","year":"2010","journal-title":"Flora"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1007\/s00606-014-1100-2","article-title":"Morphological, histological and ultrastructural features of osmophores and nectary of Bulbophyllum wendlandianum (Kraenzl.) Dammer (B. section Cirrhopetalum Lindl., Bulbophyllinae Schltr., Orchidaceae)","volume":"301","author":"Kowalkowska","year":"2015","journal-title":"Plant Syst. Evol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1002\/j.1537-2197.1983.tb12472.x","article-title":"Ultrastructure of osmophores in Restrepia (Orchidaceae)","volume":"70","author":"Pridgeon","year":"1983","journal-title":"Am. J. Bot."},{"key":"ref_66","unstructured":"Buchanan, B.B., Gruissem, W., and Jones, R.L. (2015). Biochemistry and Molecular Biology of Plants, Wiley-Blackwell."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1105\/tpc.105.039859","article-title":"Plastoglobules are lipoprotein subcompartments of the chloroplast that are permanently coupled to thylakoid membranes and contain biosynthetic enzymes","volume":"18","author":"Austin","year":"2006","journal-title":"Plant Cell"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.tplants.2007.04.003","article-title":"Plastoglobules: Versatile lipoprotein particles in plastids","volume":"12","author":"Kessler","year":"2007","journal-title":"Trends Plant Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.flora.2006.08.003","article-title":"Ultrastructure of the corona of scented and scentless flowers of Passiflora spp. (Passifloraceae)","volume":"202","author":"Galati","year":"2007","journal-title":"Flora"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2937","DOI":"10.1093\/jxb\/erl053","article-title":"Thermodynamics of the water permeability of plant cuticles: Characterization of the polar pathway","volume":"57","author":"Riederer","year":"2006","journal-title":"J. Exp. Bot."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1002\/j.1537-2197.1987.tb08747.x","article-title":"Osmophores of Stanhopea (Orchidaceae)","volume":"74","author":"Stern","year":"1987","journal-title":"Am. J. Bot."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1007\/s00606-012-0611-y","article-title":"Studies on the ultrastructure of a three-spurred fumeauxiana form of Anacamptis pyramidalis","volume":"298","author":"Kowalkowska","year":"2012","journal-title":"Plant Syst. Evol."},{"key":"ref_73","first-page":"91","article-title":"Osmophores of the fragrant orchid Gymnadenia conopsea L. (Orchidaceae)","volume":"70","year":"2001","journal-title":"Acta Soc. Bot. Pol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1002\/ffj.70057","article-title":"Scent-derived bioactivity of Myosotis sylvatica flowers: GC\u2013MS-based volatile profiling and computational evaluation of antioxidant targets","volume":"41","author":"Aytar","year":"2026","journal-title":"Flavour Fragr. J."},{"key":"ref_75","unstructured":"Mowszowicz, J. (1980). Przewodnik do Oznaczania Krajowych Ro\u015blin Zielarskich Pa\u0144stwowe Wydawnictwo Rolnicze i Le\u015bne, Pa\u0144stwowe Wydawnictwo Rolnicze i Le\u015bne (PWRiL)."},{"key":"ref_76","first-page":"593","article-title":"Content of flavonoids in plant species of genus Myosotis L.","volume":"16","author":"Shinkarenko","year":"2008","journal-title":"Chem. Sustain. Dev."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1007\/s10600-008-9130-z","article-title":"Phenolcarboxylic acids from Myosotis krylovii and M. palustris","volume":"44","author":"Shinkarenko","year":"2008","journal-title":"Chem. Nat. Compd."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1007\/s10517-011-1393-x","article-title":"Anxiolytic and antidepressant effects of aqueous tincture of the aerial part of Myosotis arvensis","volume":"151","author":"Polomeyeva","year":"2011","journal-title":"Bull. Exp. Biol. Med."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"198","DOI":"10.26538\/tjnpr\/v2i4.8","article-title":"In vivo evaluation of antiplasmodial properties of Myosotis scorpioides L. (Boraginaceae) extract in albino mice infected with Plasmodium berghei","volume":"2","author":"Zakari","year":"2018","journal-title":"Trop. J. Nat. Prod. Res."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"127","DOI":"10.59277\/RJB-PB.2025.1-2.10","article-title":"Terapeutic effects of the species Myosotis sylvatica","volume":"70","author":"Isac","year":"2025","journal-title":"Rom. J. Biol.-Plant Biol."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Bussmann, R.W. (2024). Myosotis arvensis (L.) Hill Boraginaceae. Ethnobotany of the Caucasus. Ethnobotany of Mountain Regions, Springer.","DOI":"10.1007\/978-3-319-50009-6_215-1"},{"key":"ref_82","first-page":"68","article-title":"Isolation and characterization of flavonoids from Myosotis scorpioides L. (Boraginaceae) and evaluation of antimalarial efficacy of dichloromethane extract of the plant","volume":"10","author":"Abdu","year":"2025","journal-title":"Jewel J. Sci. Res."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"9265929","DOI":"10.1155\/2024\/9265929","article-title":"Lesser-explored edible flowers as a choice of phytochemical sources for food applications","volume":"2024","year":"2024","journal-title":"Int. J. Food Sci."},{"key":"ref_84","unstructured":"Mirek, Z., Pi\u0119ko\u015b-Mirkowa, H., Zaj\u0105c, A., and Zaj\u0105c, M. (2020). Vascular Plants of Poland. An Annotated Checklist [Ro\u015bliny Naczyniowe Polski. Adnotowany Wykaz Gatunk\u00f3w], W. Szafer Institute of Botany, Polish Academy of Sciences."},{"key":"ref_85","unstructured":"Hall, J.L. (1978). An introduction to techniques for scanning electron microscopy of plant cells. Electron Microscopy and Cytochemistry of Plant Cells, Elsevier."},{"key":"ref_86","unstructured":"Broda, B. (1971). Metody Histochemii Ro\u015blinnej, Pa\u0144stwowy Zak\u0142ad Wydawnictw Lekarskich."},{"key":"ref_87","unstructured":"Johansen, D.A. (1940). Plant Microtechnique, McGraw Hill. [1st ed.]."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"111","DOI":"10.3109\/10520299109110562","article-title":"Efficient lipid staining in plant material with Sudan Red 7B or fluorol yellow 088 in polyethylene glycol\u2013glycerol","volume":"66","author":"Brundrett","year":"1991","journal-title":"Biotech. Histochem."},{"key":"ref_89","unstructured":"Baker, J.R. (1966). Cytological Technique, Methven. [5th ed.]."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/j.1365-2818.1995.tb03642.x","article-title":"Improved staining procedures for photographic documentation of phenolic deposits in semithin sections of plant tissue","volume":"179","author":"Gutmann","year":"1995","journal-title":"J. Microsc."},{"key":"ref_91","unstructured":"O\u2019Brien, T.P., and McCully, M.E. (1981). The Study of Plant Structure: Principles and Selected Methods, Thermacarphi Pty Ltd."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1083\/jcb.17.1.208","article-title":"The use of lead citrate at high pH as an electronopaque stain in electron microscopy","volume":"17","author":"Reynolds","year":"1963","journal-title":"J. Cell. Biol."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2658\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T03:05:54Z","timestamp":1788145554000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,30]]},"references-count":92,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["plants15172658"],"URL":"https:\/\/doi.org\/10.3390\/plants15172658","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,30]]}}}