{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:07:53Z","timestamp":1788930473110,"version":"build-2803163510"},"reference-count":43,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008871","name":"Yunnan Province Science and Technology Department","doi-asserted-by":"publisher","award":["202402AE090017"],"award-info":[{"award-number":["202402AE090017"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]},{"award":["202402AE090017"],"award-info":[{"award-number":["202402AE090017"]}],"id":[{"id":"https:\/\/ror.org\/03whqdf70","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Potato cyst nematode (PCN; Globodera spp.) is an internationally quarantined pest threatening potato production in Yunnan, Sichuan, and Guizhou, China. We screened 418 potato accessions using markers 57R (H1), Gro1-4(Gro1-4), and Gpa2-2(Gpa2). Detection frequencies were 40.43% (Gpa2-2), 14.35% (57R), and 3.11% (Gro1-4); 50.48% carried at least one marker, 7.42% carried two markers, and none carried all three. Based on marker genotypes, nine representative accessions (five H1-carrying, two Gpa2-only, two marker-free) were field-tested in a nursery infested with Globodera rostochiensis. All five H1-carrying accessions showed no cyst formation (RF = 0), indicating high resistance. The Gpa2-only accessions had RF values of 0.37 and 0.58, indicating partial resistance, although Gpa2 mainly targets Globodera pallida. Marker-free accessions were susceptible (RF \u2265 0.79). According to international criteria (RF &lt; 0.1 considered highly resistant), five accessions\u2014\u2018Dianshu 1208\u2019, \u2018Dianshu 14023\u2019, \u2018Dianshu 1520\u2019, \u2018Diancaishu 102\u2019, and \u2018Atlantic\u2019\u2014were confirmed to be highly resistant. These results indicate that H1 is associated with effective resistance to G. rostochiensis in the tested accessions and highlight the value of combining marker-assisted selection with field validation for reliable PCN resistance identification.<\/jats:p>","DOI":"10.3390\/plants15172686","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:41:18Z","timestamp":1788266478000},"page":"2686","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Screening and Field Validation of Potato Germplasm for Resistance to Potato Cyst Nematodes"],"prefix":"10.3390","volume":"15","author":[{"given":"Xi","family":"Yang","sequence":"first","affiliation":[{"name":"College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongming","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanchao","family":"Yue","sequence":"additional","affiliation":[{"name":"College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoyan","family":"Song","sequence":"additional","affiliation":[{"name":"College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huachun","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1007\/s42360-024-00813-2","article-title":"Histopathological and histochemical alterations resulting from Phytophthora infestans infection in the potato plant Solanum tuberosum (Solanaceae)","volume":"77","year":"2024","journal-title":"Indian Phytopathol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Xiao, C., Huang, M., Gao, J., Wang, Z., Zhang, D., Zhang, Y., Yan, L., Yu, X., Li, B., and Shen, Y. (2020). Comparative proteomics of three Chinese potato cultivars to improve understanding of potato molecular response to late blight disease. BMC Genom., 21.","DOI":"10.1186\/s12864-020-07286-3"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Rashid, R.A.M., Liang, S., Sahito, A.Z., Yang, J., Li, Z., Li, Z., Hu, Z., Pan, Z., and Sui, Q. (2025). Improving the prediction of potato yield gaps: Solanum-model parameterization and evaluation in southwestern China. PLoS ONE, 20.","DOI":"10.1371\/journal.pone.0328675"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Sood, S., Bhardwaj, V., Bairwa, A., Dalamu Sharma, S., Sharma, A.K., Kumar, A., Lal, M., and Kumar, V. (2023). Genome-wide association mapping and genomic prediction for late blight and potato cyst nematode resistance in potato (Solanum tuberosum L.). Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1211472"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ympev.2013.05.020","article-title":"Genetic diversity of the golden potato cyst nematode Globodera rostochiensis and determination of the origin of populations in Quebec","volume":"69","author":"Boucher","year":"2013","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"13915","DOI":"10.1038\/s41598-024-64617-0","article-title":"Europe as a secondary distribution hub in the worldwide invasion of the potato cyst nematode Globodera rostochiensis","volume":"14","author":"Esquibet","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1111\/mpp.13047","article-title":"Potato cyst nematodes Globodera rostochiensis and G. pallida","volume":"22","author":"Barone","year":"2021","journal-title":"Mol. Plant Pathol."},{"key":"ref_8","first-page":"290","article-title":"Life Cycle of the Golden Cyst Nematode, Globodera rostochiensis, in Quebec, Canada","volume":"47","author":"Benjamin","year":"2015","journal-title":"J. Nematol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1134\/S1062359019030142","article-title":"Expression and Functional Characteristics of Genes in the Globodera rostochiensis Woll. Potato Cyst Nematode at Different Stages of Its Life Cycle","volume":"46","author":"Matveeva","year":"2019","journal-title":"Biol. Bull."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1094\/PDIS-10-21-2231-RE","article-title":"Association of Globodera rostochiensis (Nematoda) with stunted and chlorotic potato plants in Yunnan and Sichuan Provinces in China","volume":"107","author":"Jiang","year":"2022","journal-title":"Plant Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6078917","DOI":"10.1155\/2020\/6078917","article-title":"The Possible Biotechnological Use of Edible Mushroom Bioproducts for Controlling Plant and Animal Parasitic Nematodes","volume":"2020","author":"Zamilpa","year":"2020","journal-title":"BioMed Res. Int."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"104420","DOI":"10.1016\/j.biocontrol.2020.104420","article-title":"Biological control of sheep nematode Haemonchus contortus using edible mushrooms","volume":"152","year":"2021","journal-title":"Biol. Control"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Akhtar, M.S., and Hakeem, K.R. (2025). Progress and Development of Plant Nematology and Future Consequences. Plant-Nematode Interactions and Their Management Strategies, Apple Academic Press.","DOI":"10.1201\/9781779643285"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1002\/csc2.21244","article-title":"Breeding for potato cyst nematode resistance in Solanum tuberosum","volume":"64","author":"Spychalla","year":"2024","journal-title":"Crop Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/BF00271550","article-title":"Localization by restriction fragment length polymorphism mapping in potato of a major dominant gene conferring resistance to the potato cyst nematode Globodera rostochiensis","volume":"224","author":"Barone","year":"1990","journal-title":"Mol. Gen. Genet."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1007\/s12230-019-09745-4","article-title":"QTL for Resistance to Globodera rostochiensis Pathotype Ro2 and G. pallida Pathotype Pa2\/3 in Autotetraploid Potato","volume":"96","author":"Park","year":"2019","journal-title":"Am. J. Potato Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1046\/j.1365-313X.2002.01341.x","article-title":"The broad-spectrum potato cyst nematode resistance gene (Hero) from tomato is the only member of a large gene family of NBS-LRR genes with an unusual amino acid repeat in the LRR region","volume":"31","author":"Ernst","year":"2002","journal-title":"Plant J."},{"key":"ref_18","first-page":"173","article-title":"Wild relatives and interspecific hybrids of potato as source materials in breeding for resistance to golden nematode","volume":"181","author":"Mironenko","year":"2020","journal-title":"Proc. Appl. Bot."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/BF00220911","article-title":"Identification of RFLP markers closely linked to the H1 gene conferring resistance to Globodera rostochiensis in potato","volume":"85","author":"Gebhardt","year":"1993","journal-title":"Theor. Appl. Genet."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3882","DOI":"10.1038\/s41598-017-03871-x","article-title":"Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida","volume":"7","author":"Emilio","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1007\/s11540-020-09459-9","article-title":"Solanum gourlayi\u2014A Source of Cyst Nematode Resistance in Potato Breeding","volume":"63","author":"Milczarek","year":"2020","journal-title":"Potato Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.molbiopara.2004.03.001","article-title":"Novel matrix metalloproteinase from the cyst nematodes Heterodera glycines and Globodera rostochiensis","volume":"136","author":"Kovaleva","year":"2004","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1046\/j.1364-3703.2003.00140.x","article-title":"Characterization of a chorismate mutase from the potato cyst nematode Globodera pallida","volume":"4","author":"Jones","year":"2003","journal-title":"Mol. Plant Pathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1007\/s10681-019-2413-6","article-title":"The Rx gene derived USDA 41956 and Rx1 gene derived CPC 1673 confer equal resistance to the migration of Potato virus X from potato leaves to tubers","volume":"215","author":"Ohbayashi","year":"2019","journal-title":"Euphytica"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3725\/jjn.45.115","article-title":"Detection of the potato cyst nematode resistance gene Hero A in Japanese tomato cultivars by the PCR-RFLP method","volume":"45","author":"Uehara","year":"2015","journal-title":"Nematol. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1270\/jsbbs.22094","article-title":"Studies of potato resistance to Globodera rostochiensis revealed novel alleles for 57R marker","volume":"73","author":"Anna","year":"2023","journal-title":"Breed. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1094\/PDIS-09-24-2040-RE","article-title":"Evaluating Resistance of Potato Cultivars and Breeding Clones to Golden Cyst Nematode Globodera rostochiensis in China","volume":"109","author":"Jiang","year":"2025","journal-title":"Plant Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1270\/jsbbs.20134","article-title":"Improvement of diagnostic markers for resistance to Globodera pallida and application for selection of resistant germplasms in potato breeding","volume":"71","author":"Asano","year":"2021","journal-title":"Breed. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1007\/s00122-009-1185-0","article-title":"Development of diagnostic markers for use in breeding potatoes resistant to Globodera pallida pathotype Pa2\/3 using germplasm derived from Solanum tuberosum ssp. andigena CPC 2802","volume":"120","author":"Claire","year":"2010","journal-title":"Theor. Appl. Genet."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/j.1365-313X.2004.02047.x","article-title":"Molecular cloning of the potato Gro1-4 gene conferring resistance to pathotype Ro1 of the root cyst nematode Globodera rostochiensis, based on a candidate gene approach","volume":"38","author":"Paal","year":"2004","journal-title":"Plant J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1270\/jsbbs.62.142","article-title":"DNA marker-assisted evaluation of potato genotypes for potential resistance to potato cyst nematode pathotypes not yet invading into Japan","volume":"62","author":"Asano","year":"2012","journal-title":"Breed. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"365","DOI":"10.3103\/S1068367408060013","article-title":"Use of molecular markers of potato golden nematode resistance genes H1 and GRO1","volume":"34","author":"Biryukova","year":"2008","journal-title":"Russ. Agric. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1007\/s001220050638","article-title":"Mapping of the cyst nematode resistance locus Gpa2 in potato using a strategy based on comigrating AFLP markers","volume":"95","author":"Rouppe","year":"1997","journal-title":"Theor. Appl. Genet."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1046\/j.1365-313x.2000.00814.x","article-title":"Homologues of a single resistance-gene cluster in potato confer resistance to distinct pathogens: A virus and a nematode","volume":"23","author":"Vossen","year":"2000","journal-title":"Plant J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1134\/S160767291505004X","article-title":"The expression of R genes in genetic and induced resistance to potato cyst nematode Globodera rostochiensis (Wollenweber, 1923) Behrens, 1975","volume":"464","author":"Lavrova","year":"2015","journal-title":"Dokl. Biochem. Biophys."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1270\/jsbbs.20121","article-title":"The relations of broad nematode resistance to quality characteristics as a consequence of marker-assisted selection in potato breeding programs","volume":"71","author":"Dorota","year":"2021","journal-title":"Breed. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e70105","DOI":"10.1111\/mec.70105","article-title":"Adaptation of the Cyst Nematode Globodera pallida to the Colinear Potato Resistant QTLs GpaVvrn and GpaVspl Involved Distinct Genomic Regions and Absence of Cross-Virulence","volume":"34","author":"Lechevalier","year":"2025","journal-title":"Mol. Ecol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"101679","DOI":"10.1016\/j.xplc.2025.101679","article-title":"Interspecific allelopathy suppresses potato cyst nematodes by 1-Decanol produced from Houttuynia cordata","volume":"7","author":"Zhang","year":"2025","journal-title":"Plant Commun."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"294","DOI":"10.3103\/S0095452713050058","article-title":"Allelic state of the molecular marker for golden nematode (Globodera rostochiensis) resistance gene H1 among Ukrainian and world potato (Solanum tuberosum ssp. tuberosum) cultivars","volume":"47","author":"Karelov","year":"2013","journal-title":"Cytol. Genet."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.scienta.2015.11.029","article-title":"Identification of potato cyst nematode resistant genotypes using molecular markers","volume":"198","author":"Sudha","year":"2016","journal-title":"Sci. Hortic."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1007\/s11676-006-0068-3","article-title":"DNA extraction of birch leaves by improved CTAB method and optimization of its ISSR system","volume":"17","author":"Pan","year":"2006","journal-title":"J. For. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1007\/s00122-010-1472-9","article-title":"Comparative sequence analysis of the potato cyst nematode resistance locus H1 reveals a major lack of co-linearity between three haplotypes in potato (Solanum tuberosum ssp.)","volume":"122","author":"Bakker","year":"2011","journal-title":"Theor. Appl. Genet."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1515\/plass-2017-0014","article-title":"Evaluation of Potato Cultivars and Breeding Lines for Resistance to Globodera rostochiensis and Globodera pallida","volume":"76","author":"Przetakiewicz","year":"2017","journal-title":"Plant Breed. Seed Sci."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2686\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T04:24:32Z","timestamp":1788927872000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/17\/2686"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":43,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["plants15172686"],"URL":"https:\/\/doi.org\/10.3390\/plants15172686","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]}}}