{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:18:03Z","timestamp":1788430683685,"version":"build-2803163510"},"reference-count":59,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"2023 Young Teacher Funding Project of Zhoukou Normal University","award":["ZKNUC2023081"],"award-info":[{"award-number":["ZKNUC2023081"]}]},{"award":["ZKNUC2023081"],"award-info":[{"award-number":["ZKNUC2023081"]}],"id":[{"id":"https:\/\/ror.org\/00jjkh886","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Science and Technology Research Project of Henan Province","award":["262102110178"],"award-info":[{"award-number":["262102110178"]}]},{"name":"Central Guiding Local Science and Technology Development Fund Project in Henan Province","award":["Z20251831182"],"award-info":[{"award-number":["Z20251831182"]}]},{"name":"Key Research and Development Project of Henan Province","award":["251111110900"],"award-info":[{"award-number":["251111110900"]}]},{"name":"Henan Province Science and Technology Research and Development Joint Fund (Industry) Major Project","award":["235101610015"],"award-info":[{"award-number":["235101610015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Genes encoding DUF568 domain-containing proteins participate in plant stress adaptation. To elucidate the functional role of DUF568 domain-containing genes in Trichoderma-induced wheat defense responses against wheat Fusarium crown rot, we performed a genome-wide identification and characterization of the TaDUF568 gene family in hexaploid wheat (Triticum aestivum L.). In this study, a total of 33 TaDUF568 family genes were systematically identified and characterized at the genome-wide level, exhibiting uneven chromosomal distribution and diverse physicochemical properties. Phylogenetic, structural, and collinearity analyses revealed conserved family characteristics among monocot species. Segmental duplication was verified as the primary driver of gene family expansion. Expression profiling revealed divergent tissue-specific expression patterns among TaDUF568 family members, among which TaDUF568.18 was strongly induced by Trichoderma M2. Subcellular localization assays confirmed that TaDUF568.18 is a plasma membrane-localized protein. Functional validation via stable transgenes demonstrated that overexpression of TaDUF568.18 restricted lesion expansion, improved agronomic traits, and enhanced disease resistance. This study is the first to characterize the wheat DUF568 family and confirm that TaDUF568.18 (annotated as TaAIR12) acts as a positive regulator of Trichoderma-mediated wheat defense, providing a valuable gene resource for wheat disease-resistance breeding.<\/jats:p>","DOI":"10.3390\/plants15172705","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:20:43Z","timestamp":1788427243000},"page":"2705","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Key Trichoderma-Induced Gene Encoding a DUF568 Domain-Containing Protein Mediates Defense Responses in Wheat"],"prefix":"10.3390","volume":"15","author":[{"given":"Junchang","family":"Li","sequence":"first","affiliation":[{"name":"Henan Province Key Laboratory of Efficient Crop Production and Food Quality Safety, Zhoukou Normal University, Zhoukou 466001, China"},{"name":"Modern Agricultural Industry Research Institute, Henan Zhoukou National Agricultural High-Tech Industry Demonstration Zone, Zhoukou 477100, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Yingying","family":"Jin","sequence":"additional","affiliation":[{"name":"Modern Agricultural Industry Research Institute, Henan Zhoukou National Agricultural High-Tech Industry Demonstration Zone, Zhoukou 477100, China"},{"name":"Wheat Technology Innovation Center, Henan Zhoukou Agricultural High-Tech Industry Demonstration Zone, Zhoukou 477100, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Yingxue","family":"Wang","sequence":"additional","affiliation":[{"name":"Modern Agricultural Industry Research Institute, Henan Zhoukou National Agricultural High-Tech Industry Demonstration Zone, Zhoukou 477100, China"},{"name":"Wheat Technology Innovation Center, Henan Zhoukou Agricultural High-Tech Industry Demonstration Zone, Zhoukou 477100, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Tingyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Modern Agricultural Industry Research Institute, Henan Zhoukou National Agricultural High-Tech Industry Demonstration Zone, Zhoukou 477100, China"},{"name":"Wheat Technology Innovation Center, Henan Zhoukou Agricultural High-Tech Industry Demonstration Zone, Zhoukou 477100, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Huifang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Henan Province Key Laboratory of Efficient Crop Production and Food Quality Safety, Zhoukou Normal University, Zhoukou 466001, China"},{"name":"Modern Agricultural Industry Research Institute, Henan Zhoukou National Agricultural High-Tech Industry Demonstration Zone, Zhoukou 477100, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Yongjing","family":"Ni","sequence":"additional","affiliation":[{"name":"Shangqiu Academy of Agricultural and Forestry Sciences, Shangqiu 476000, China"},{"name":"The Suihuang Laboratory, Shangqiu 476000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"visualization","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lv, P., Wan, J., Zhang, C., Hina, A., Al Amin, G., Begum, N., and Zhao, T. 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