{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:20:18Z","timestamp":1788456018221,"version":"build-2803163510"},"reference-count":46,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Basic Research Funds of Hebei Academy of Agricultural and Forestry Sciences","award":["2025020105"],"award-info":[{"award-number":["2025020105"]}]},{"name":"HAAFS Science and Technology Innovation Special Project","award":["2026KJCXZX-CGS-4"],"award-info":[{"award-number":["2026KJCXZX-CGS-4"]}]},{"name":"Special Program for Modern Agricultural Science and Technology Innovation","award":["262N6308D"],"award-info":[{"award-number":["262N6308D"]}]},{"name":"China Agriculture Research System","award":["CARS-29-yc-8"],"award-info":[{"award-number":["CARS-29-yc-8"]}]},{"award":["CARS-29-yc-8"],"award-info":[{"award-number":["CARS-29-yc-8"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Innovation Team of Hebei Modern Agricultural Industrial Technology System","award":["HBCT2023150202"],"award-info":[{"award-number":["HBCT2023150202"]}]},{"name":"Science and Technology Innovation Talent Team Construction Project of Hebei Academy of Agriculture and Forestry Sciences","award":["C26R0607"],"award-info":[{"award-number":["C26R0607"]}]},{"award":["C26R0607"],"award-info":[{"award-number":["C26R0607"]}],"id":[{"id":"https:\/\/ror.org\/051p3cy55","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The muscat aroma of grape is largely determined by terpenoid volatiles. However, its genetic basis in \u2018Muscat Hamburg\u2019 remains unclear. We performed comparative volatile metabolomics between \u2018Muscat Hamburg\u2019 and \u2018Red Globe\u2019 across berry development, identifying 127 differentially abundant metabolites, with upregulated ones enriched in the monoterpenoid pathway. Using an F1 segregating population (264 progeny), we quantified four key monoterpenoids (\u03b1-terpineol, linalool, geraniol, and cis-linalool oxide) over two consecutive years. Using a high-density genetic map with 7872 SNP markers generated via SLAF-seq, we detected stable major QTLs for \u03b1-terpineol, linalool, and geraniol on LG5, and a novel QTL for cis-linalool oxide on LG14. Expression analysis revealed that the transcript levels of VvDXS and VvGGPPS1 were significantly higher in \u2018Muscat Hamburg\u2019 than in \u2018Red Globe\u2019 at ripening. Heterologous overexpression in tomato enhanced terpenoid aroma accumulation without affecting fruit development. These results indicate that VvDXS and VvGGPPS1 play a crucial role in the formation of the aroma in the \u2018Muscat Hamburg\u2019 variety.<\/jats:p>","DOI":"10.3390\/plants15172708","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T16:21:21Z","timestamp":1788452481000},"page":"2708","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["GC-MS-Based Volatile Metabolomics and Quantitative Trait Locus Mapping of Aroma Components in \u2018Muscat Hamburg\u2019 Grape"],"prefix":"10.3390","volume":"15","author":[{"given":"Minmin","family":"Li","sequence":"first","affiliation":[{"name":"Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, College of Agriculture, Shihezi University, Shihezi 832000, China"},{"name":"Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, Qinhuangdao 066600, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6128-8240","authenticated-orcid":false,"given":"Peng-Tao","family":"Shi","sequence":"additional","affiliation":[{"name":"Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, Qinhuangdao 066600, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6343-7128","authenticated-orcid":false,"given":"Nan","family":"Jia","sequence":"additional","affiliation":[{"name":"Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli, Qinhuangdao 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