{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:20:35Z","timestamp":1788456035161,"version":"build-2803163510"},"reference-count":71,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872998"],"award-info":[{"award-number":["31872998"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["31872998"],"award-info":[{"award-number":["31872998"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Crop productivity around the world is largely constrained by salt-induced stress, a key abiotic factor. Although oat (Avena sativa L.) can withstand challenging environmental conditions, the physiological and molecular responses underlying salt tolerance during germination and early seedling development remain insufficiently understood. To investigate these responses, 28 oat varieties were evaluated at the germination stage, and two contrasting varieties, the salt-tolerant Mengshi No. 1 (MS) and salt-sensitive Morgan (MG), were selected for detailed analysis under a severe NaCl treatment (300 mM) during early seedling stages. Under severe salt stress, the two oat varieties exhibited distinct growth and physiological responses, including changes in growth traits, chlorophyll content, membrane stability, osmotic adjustment, and antioxidant responses. Transcriptomic analysis revealed 14,109 differentially expressed genes (DEGs) between salt-treated MG and its respective control (CK), 19,405 between salt-treated MS and its CK, and 6161 between salt-treated MG and salt-treated MS, suggesting different transcriptional response patterns between the salt-tolerant and salt-sensitive varieties under severe salt stress. Weighted gene co-expression network analysis (WGCNA) revealed a salt-responsive module associated with MS, from which five hub genes, AVESA.00010b.r2.1CG0087930 (MGL), AVESA.00010b.r2.19DG0180280 (MGL), AVESA.00010b.r2.4CG1272260 (BCH1), AVESA.00010b.r2.5DG0989800 (GPAT7), and AVESA.00010b.r2.6CG1124100 (TPR10), were identified as candidate genes potentially associated with salt tolerance and stress responses.<\/jats:p>","DOI":"10.3390\/plants15172712","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T16:21:21Z","timestamp":1788452481000},"page":"2712","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrative Physiological and Transcriptomic Analyses of Oat (Avena sativa L.) Seedlings Under Severe Salt Stress"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1850-1298","authenticated-orcid":false,"given":"Rui","family":"Qiu","sequence":"first","affiliation":[{"name":"Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Xinyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Xiangpeng","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Wenjie","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Qianhan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Yingying","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Muzhapaer","family":"Tuluohong","sequence":"additional","affiliation":[{"name":"Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Baiji","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Guowen","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3866-2707","authenticated-orcid":false,"given":"Bing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"167179","DOI":"10.1016\/j.scitotenv.2023.167179","article-title":"Effective strategies for reclamation of saline-alkali soil and response mechanisms of the soil-plant system","volume":"905","author":"Xu","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_2","first-page":"1233","article-title":"The present situation and hot issues in the salt-affected soil research","volume":"67","author":"Li","year":"2012","journal-title":"Acta Geogr. Sin."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Liu, C., Jiang, X., and Yuan, Z. (2024). Plant responses and adaptations to salt stress: A review. Horticulturae, 10.","DOI":"10.3390\/horticulturae10111221"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1006617","DOI":"10.3389\/fpls.2022.1006617","article-title":"Salt stress resilience in plants mediated through osmolyte accumulation and its crosstalk mechanism with phytohormones","volume":"13","author":"Singh","year":"2022","journal-title":"Front. Plant Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Bai, J., Lu, P., Li, F., Li, L., and Yin, Q. (2023). Metabolome and transcriptome analyses reveal the differences in the molecular mechanisms of oat leaves responding to salt and alkali stress conditions. Agronomy, 13.","DOI":"10.3390\/agronomy13061441"},{"key":"ref_6","first-page":"253","article-title":"Growth and yield of oat (Avena sativa L.) cv. Kent under Na2SO4 dominated saline soil","volume":"13","author":"Mishra","year":"1986","journal-title":"Geobios"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Huang, H., Wang, X., Li, J., Gao, Y., Yang, Y., Wang, R., Zhou, Z., Wang, P., and Zhang, Y. (2024). Trends and directions in oats research under drought and salt stresses: A bibliometric analysis (1993\u20132023). Plants, 13.","DOI":"10.3390\/plants13141902"},{"key":"ref_8","first-page":"10","article-title":"Research on salt-affected soils in China: History, status quo and prospect","volume":"59","author":"Yang","year":"2022","journal-title":"Acta Pedol. Sin."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Wang, Q., Liang, X., Xiang, D., Xu, W., Wang, C., Zhan, C., Ren, C., Wei, L., Zhang, S., and Zhang, L. (2023). The Physiological Mechanism of Melatonin Enhancing the Tolerance of Oat Seedlings under Saline\u2013Alkali Stress. Agronomy, 13.","DOI":"10.3390\/agronomy13092343"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1439-037X.1986.tb00016.x","article-title":"Salt Tolerance of Some Oats (Avena sativa L.) Varieties at Germination and Seedling Stage","volume":"156","author":"Verma","year":"1986","journal-title":"J. Agron. Crop Sci."},{"key":"ref_11","first-page":"1960","article-title":"Salt-Tolerance of Seed Germination and Seedling Growth of Different Oat Varieties","volume":"28","author":"Wu","year":"2009","journal-title":"Chin. J. Ecol."},{"key":"ref_12","first-page":"101","article-title":"Effect of Secondary Salinization on Photosynthesis in Fodder Oat (Avena sativa L.) Genotypes","volume":"185","author":"Chatrath","year":"2000","journal-title":"J. Agron. Crop Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"123","DOI":"10.2135\/cropsci2006.06.0371","article-title":"Growth, Gas Exchange, Chlorophyll Fluorescence, and Ion Content of Naked Oat in Response to Salinity","volume":"47","author":"Zhao","year":"2007","journal-title":"Crop Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"468","DOI":"10.15835\/nbha4329972","article-title":"Effects of NaCl Stress on the Growth and Physiological Changes in Oat (Avena sativa) Seedlings","volume":"43","author":"Mu","year":"2015","journal-title":"Not. Bot. Horti Agrobot. Cluj-Napoca"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"109","DOI":"10.17138\/tgft(9)109-119","article-title":"Salinity Tolerance of Avena sativa Fodder Genotypes","volume":"9","author":"Roy","year":"2021","journal-title":"Trop. Grassl.-Forrajes Trop."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"153708","DOI":"10.1016\/j.jplph.2022.153708","article-title":"Evaluation of Salt Tolerance of Oat Cultivars and the Mechanism of Adaptation to Salinity","volume":"273","author":"Zhang","year":"2022","journal-title":"J. Plant Physiol."},{"key":"ref_17","first-page":"3249","article-title":"Physiological responses and adoptive mechanisms in oat against three levels of salt stress","volume":"51","author":"Gao","year":"2023","journal-title":"Not. Bot. Horti Agrobot. Cluj-Napoca"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Chen, X., Xu, Z., Zhao, B., Yang, Y., Mi, J., Zhao, Z., and Liu, J. (2022). Physiological and proteomic analysis responsive mechanisms for salt stress in oat. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.891674"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"D1124","DOI":"10.1093\/nar\/gku1042","article-title":"GeneFriends: A human RNA-seq-based gene and transcript co-expression database","volume":"43","author":"Craig","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.pbiomolbio.2019.02.005","article-title":"Integrative meta-analysis of transcriptomic responses to abiotic stress in cotton","volume":"146","author":"Tahmasebi","year":"2019","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Chen, J., Zhang, L., Liu, Y., Shen, X., Guo, Y., and Ma, X. (2024). RNA-seq-based WGCNA and association analysis reveal the key regulatory module and genes responding to salt stress in wheat roots. Plants, 13.","DOI":"10.3390\/plants13020274"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Pan, L., Chen, Y., Ren, Z., Khojely, D.M., Wang, S., Li, Y., Ibrahim, S.E., Fan, S., and Wei, J. (2025). Using WGCNA and transcriptome profiling to identify hub genes for salt stress tolerance in germinating soybean seeds. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1569565"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"530","DOI":"10.3389\/fpls.2019.00530","article-title":"An effective screening method and a reliable screening trait for salt tolerance of Brassica napus at the germination stage","volume":"10","author":"Wu","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_24","first-page":"1","article-title":"Impact of salinity on seed germination and seedling growth of wheat","volume":"21","author":"Hossain","year":"2024","journal-title":"J. Sci. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s10725-025-01305-7","article-title":"Hormonal signaling at seed germination and seedling stage of plants under salinity stress","volume":"105","author":"Ghosh","year":"2025","journal-title":"Plant Growth Regul."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Manono, B.O. (2026). Effects of salinity on seed germination: Mechanisms, impacts, and mitigation strategies. Seeds, 5.","DOI":"10.3390\/seeds5010001"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s00425-024-04429-8","article-title":"BR regulates wheat root salt tolerance by maintaining ROS homeostasis","volume":"260","author":"Hou","year":"2024","journal-title":"Planta"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Jadidi, O., Etminan, A., Azizi-Nezhad, R., Ebrahimi, A., and Pour-Aboughadareh, A. (2022). Physiological and Molecular Responses of Barley Genotypes to Salinity Stress. Genes, 13.","DOI":"10.3390\/genes13112040"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.plaphy.2020.08.042","article-title":"Salinity induced physiological and biochemical changes in plants: An omic approach towards salt stress tolerance","volume":"156","author":"Arif","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.plaphy.2021.04.024","article-title":"Post-stress restorative response of two quinoa genotypes differing in their salt resistance after salinity release","volume":"164","author":"Derbali","year":"2021","journal-title":"Plant Physiol. Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Maimaiti, A., Gu, W., Yu, D., Guan, Y., Qu, J., Qin, T., Ren, J., Zheng, H., and Wu, P. (2025). Dynamic molecular regulation of salt stress responses in maize (Zea mays L.) seedlings. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1535943"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Stadnik, B., Tobiasz-Salach, R., and Mazurek, M. (2023). Effect of silicon on oat salinity tolerance: Analysis of the epigenetic and physiological response of plants. Agriculture, 13.","DOI":"10.3390\/agriculture13010081"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1071\/AR03032","article-title":"Effect of sodium exclusion trait on chlorophyll retention and growth of durum wheat in saline soil","volume":"54","author":"Husain","year":"2003","journal-title":"Aust. J. Agric. Res."},{"key":"ref_34","first-page":"1513","article-title":"The effect of salt stress on growth, chlorophyll content, proline and nutrient accumulation, and K+\/Na+ ratio in walnut","volume":"44","author":"Akca","year":"2012","journal-title":"Pak. J. Bot."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Yin, Y., Fan, S., Li, S., Amombo, E., and Fu, J. (2023). Involvement of cell cycle and ion transferring in the salt stress responses of alfalfa varieties at different development stages. BMC Plant Biol., 23.","DOI":"10.1186\/s12870-023-04335-3"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.scienta.2010.09.020","article-title":"Effect of arbuscular mycorrhizal fungi on growth, mineral nutrition, antioxidant enzymes activity and fruit yield of tomato grown under salinity stress","volume":"127","author":"Latef","year":"2011","journal-title":"Sci. Hortic."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3099","DOI":"10.2134\/agronj2019.05.0347","article-title":"Ameliorative effects of jasmonic acid and humic acid on antioxidant enzymes and salt tolerance of forage sorghum under salinity conditions","volume":"111","author":"Ali","year":"2019","journal-title":"Agron. J."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Xu, M., Xu, Z., Liu, Y., Liu, Y., Liu, J., and Zhang, W. (2024). Transcriptome analysis reveals the vital role of ABA plays in drought tolerance of the ABA-insensitive alfalfa. Agronomy, 14.","DOI":"10.3390\/agronomy14030406"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.plaphy.2020.02.030","article-title":"Exogenous vanillic acid enhances salt tolerance of tomato: Insight into plant antioxidant defense and glyoxalase systems","volume":"150","author":"Parvin","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_40","first-page":"1160","article-title":"CYP71D8L is a key regulator involved in growth and stress responses by mediating gibberellin homeostasis in Qubit 3.0 Qubit 3.0","volume":"71","author":"Zhou","year":"2020","journal-title":"J. Exp. Bot."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Xiao, F., and Zhou, H. (2023). Plant salt response: Perception, signaling, and tolerance. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1053699"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1034\/j.1399-3054.2000.100410.x","article-title":"Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedlings of foxtail millet (Setaria italica)","volume":"109","author":"Sreenivasulu","year":"2000","journal-title":"Physiol. Plant."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Jing, Q., Hou, H., Meng, X., Chen, A., Wang, L., Zhu, H., Zheng, S., Lv, Z., and Zhu, X. (2022). Transcriptome analysis reveals the proline metabolic pathway and its potential regulation TF-hub genes in salt-stressed potato. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1030138"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Zhou, X., Wang, M., Yang, L., Wang, W., Zhang, Y., Liu, L., Chai, J., Liu, H., and Zhao, G. (2024). Comparative physiological and transcriptomic analyses of oat (Avena sativa) seedlings under salt stress reveal salt tolerance mechanisms. Plants, 13.","DOI":"10.3390\/plants13162238"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.cj.2021.02.010","article-title":"Salt tolerance in rice: Physiological responses and molecular mechanisms","volume":"10","author":"Liu","year":"2022","journal-title":"Crop J."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ghosh, D., and Xu, J. (2014). Abiotic stress responses in plant roots: A proteomics perspective. Front. Plant Sci., 5.","DOI":"10.3389\/fpls.2014.00006"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Chen, N., Xing, S., Song, J., Lu, S., Ling, L., and Qu, L. (2025). Transcriptome reveals the differential regulation of sugar metabolism to saline-alkali stress in different resistant oats. Genes, 16.","DOI":"10.3390\/genes16010105"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Huang, Y., Guo, Y., Liu, Y., Zhang, F., Wang, Z., Wang, H., Wang, F., Li, D., Mao, D., and Luan, S. (2018). 9-cis-epoxycarotenoid dioxygenase 3 regulates plant growth and enhances multi-abiotic stress tolerance in rice. Front. Plant Sci., 9.","DOI":"10.3389\/fpls.2018.00162"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"110188","DOI":"10.1016\/j.plantsci.2019.110188","article-title":"OsNCED5, a 9-cis-epoxycarotenoid dioxygenase gene, regulates salt and water stress tolerance and leaf senescence in rice","volume":"287","author":"Huang","year":"2019","journal-title":"Plant Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.plaphy.2019.07.023","article-title":"Comparative transcriptomics and weighted gene co-expression correlation network analysis (WGCNA) reveal potential regulation mechanism of carotenoid accumulation in Chrysanthemum \u00d7 morifolium","volume":"142","author":"Lu","year":"2019","journal-title":"Plant Physiol. Biochem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3305","DOI":"10.1007\/s00122-021-03897-w","article-title":"GWAS and WGCNA uncover hub genes controlling salt tolerance in maize (Zea mays L.) seedlings","volume":"134","author":"Ma","year":"2021","journal-title":"Theor. Appl. Genet."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Hacham, Y., Shitrit, O., Nisimi, O., Friebach, M., and Amir, R. (2023). Elucidating the importance of the catabolic enzyme, methionine-gamma-lyase, in stresses during Arabidopsis thaliana seed development and germination. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1143021"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1104\/pp.103.033431","article-title":"When defense pathways collide. The response of Arabidopsis thaliana to a combination of drought and heat stress","volume":"134","author":"Rizhsky","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1104\/pp.108.115733","article-title":"Principal transcriptional programs regulating plant amino acid metabolism in response to abiotic stresses","volume":"147","author":"Less","year":"2008","journal-title":"Plant Physiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2028","DOI":"10.1104\/pp.113.222372","article-title":"Identification of genes involved in the response of Arabidopsis to simultaneous biotic and abiotic stresses","volume":"162","author":"Atkinson","year":"2013","journal-title":"Plant Physiol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Li, T., Liu, J.X., Deng, Y.J., Xu, Z.S., and Xiong, A.S. (2021). Overexpression of a carrot bch gene, DcBCH1, improves tolerance to drought in Arabidopsis thaliana. BMC Plant Biol., 21.","DOI":"10.1186\/s12870-021-03236-7"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1023\/A:1016072218801","article-title":"Expression of a bacterial carotene hydroxylase gene (crtZ) enhances UV tolerance in tobacco","volume":"50","author":"Sandmann","year":"2002","journal-title":"Plant Mol. Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/nature00861","article-title":"Overexpression of \u03b2-carotene hydroxylase enhances stress tolerance in Arabidopsis","volume":"418","author":"Davison","year":"2002","journal-title":"Nature"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"10586","DOI":"10.1038\/s41598-024-60848-3","article-title":"Carotenoid biosynthesis genes LcLCYB, LcLCYE, and LcBCH from wolfberry confer increased carotenoid content and improved salt tolerance in tobacco","volume":"14","author":"Li","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1104\/pp.110.163741","article-title":"Characterization of the \u03b2-Carotene Hydroxylase Gene DSM2 Conferring Drought and Oxidative Stress Resistance by Increasing Xanthophylls and Abscisic Acid Synthesis in Rice","volume":"154","author":"Du","year":"2010","journal-title":"Plant Physiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.3724\/SP.J.1006.2014.01304","article-title":"Cloning and expression analysis of two homologous genes encoding sn-glycerol-3-phosphate acyltransferase 6 in Brassica napus","volume":"40","author":"Liu","year":"2014","journal-title":"Acta Agron. Sin."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.plantsci.2016.07.002","article-title":"Sunflower HaGPAT9-1 is the predominant GPAT during seed development","volume":"252","author":"Balbuena","year":"2016","journal-title":"Plant Sci."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Sui, N., Tian, S., Wang, W., Wang, M., and Fan, H. (2017). Overexpression of Glycerol-3-Phosphate Acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis. Front. Plant Sci., 8.","DOI":"10.3389\/fpls.2017.01337"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1111\/jipb.13808","article-title":"TaGPAT6 enhances salt tolerance in wheat by synthesizing cutin and suberin monomers to form a diffusion barrier","volume":"67","author":"Wang","year":"2024","journal-title":"J. Integr. Plant Biol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Li, X., Li, Y., Sun, Y., Li, S., Cai, Q., Li, S., Sun, M., Yu, T., Meng, X., and Zhang, J. (2025). Characterization of the Soybean GPAT Gene Family Identifies GmGPAT1 as a Key Protein in Salt Stress Tolerance. Plants, 14.","DOI":"10.3390\/plants14182862"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"4271","DOI":"10.1093\/jxb\/ern276","article-title":"SlTPR1, a tomato tetratricopeptide repeat protein, interacts with the ethylene receptors NR and LeETR1, modulating ethylene and auxin responses and development","volume":"59","author":"Lin","year":"2008","journal-title":"J. Exp. Bot."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1007\/s11032-017-0751-4","article-title":"Comparative functional genomics of the TPR gene family in Arabidopsis, rice and maize","volume":"37","author":"Wei","year":"2017","journal-title":"Mol. Breed."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"154415","DOI":"10.1016\/j.jplph.2024.154415","article-title":"Trimeric tetrapeptide repeat protein TPR16 positively regulates salt stress in apple","volume":"305","author":"Wang","year":"2025","journal-title":"J. Plant Physiol."},{"key":"ref_69","first-page":"1","article-title":"Screening and Evaluation of Salt Tolerance of 70 Oat Germplasm Resources at Germination Stage","volume":"46","author":"Gou","year":"2026","journal-title":"J. Triticeae Crops"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1007\/s004250050524","article-title":"Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds","volume":"207","author":"Hodges","year":"1999","journal-title":"Planta"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Wang, B., Yin, H., Zhang, X., Kong, X., Zhao, W., Qiu, R., Tuluhong, M., Cui, G., and Li, B. (2026). Deciphering Drought Response Mechanisms in Oat Through Comprehensive Transcriptomic and Physiological Analysis. 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