{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T05:02:21Z","timestamp":1789794141794,"version":"4.0.1"},"reference-count":92,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation"},{"name":"NCT Forestry"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Hybrid breeding exploits heterosis from genetically complementary parents to develop desirable products, including in Eucalyptus. The aim of this review is to present integrative techniques for hybrid breeding in Eucalyptus based on conventional methods such as phenotypic selection and controlled hybridization, and modern genomic-assisted approaches to guide product development. Molecular breeding tools such as marker-assisted selection (MAS), quantitative trait loci (QTL) mapping, genotyping-by-sequencing (GBS) and next-generation sequencing (NGS) have enhanced the precision of selection in Eucalyptus for traits such as growth rate, wood density, fibre quality, disease resistance, drought tolerance and adaptability. Modern approaches have enabled the identification of trait-specific QTL and genes in popular Eucalyptus species such as Eucalyptus grandis, E. urophylla, E. camaldulensis, and E. nitens, thereby accelerating hybrid development. Genome editing techniques offer new opportunities for genetic modification of traits, especially in Eucalyptus, where the final product may not require stringent regulatory oversight. In Eucalyptus breeding, the key market-preferred traits are pulp and paper, lumber, and bioenergy, requiring high-performance and better biomass hybrids. For successful hybrid breeding, a multidisciplinary approach is required that integrates genetic and genomic resources. Furthermore, investment in forest genomics and multi-institutional collaboration will be needed to keep up with advances in Eucalyptus breeding. The review may serve as a framework to guide Eucalyptus breeding and genetics, aiming to develop next-generation hybrids that adapt to environmental changes and meet market demands.<\/jats:p>","DOI":"10.3390\/plants15182841","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T08:03:17Z","timestamp":1789632197000},"page":"2841","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrative Techniques for Hybrid Breeding in Eucalyptus: Advances, Innovations, Challenges, and Future Prospects"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4299-4920","authenticated-orcid":false,"given":"Mncedisi","family":"Ndwandwe","sequence":"first","affiliation":[{"name":"Institute for Commercial Forestry Research, School of Agriculture and Science, University of KwaZulu-Natal, Pietermaritzburg 3201, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2793-8392","authenticated-orcid":false,"given":"Hussein","family":"Shimelis","sequence":"additional","affiliation":[{"name":"African Centre for Crop Improvement, School of Agriculture and Science, University of KwaZulu-Natal, Pietermaritzburg 3201, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1007\/s43621-025-02548-0","article-title":"Revitalizing biomass utilization for renewable energy and sustainable rural development in Japan","volume":"7","author":"Omondi","year":"2026","journal-title":"Discov. 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