{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:02:44Z","timestamp":1790053364613,"version":"4.0.1"},"reference-count":45,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Innovation Promotion Project of Shanxi Agricultural University","award":["CXGC202435"],"award-info":[{"award-number":["CXGC202435"]}],"id":[{"id":"https:\/\/ror.org\/05e9f5362","id-type":"ROR","asserted-by":"crossref"}]},{"award":["CXGC202435"],"award-info":[{"award-number":["CXGC202435"]}],"id":[{"id":"https:\/\/ror.org\/05e9f5362","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Key Research and Development Program of China","award":["2023YFD1202700"],"award-info":[{"award-number":["2023YFD1202700"]}]},{"name":"National Key Research and Development Program of China","award":["2023YFD1202703"],"award-info":[{"award-number":["2023YFD1202703"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Faba bean (Vicia faba L.) is a legume crop with diverse nutritional and bioactive properties, yet the metabolic basis underlying inter-varietal quality differences remains poorly understood. This study investigated four faba bean varieties (JD, GB, DB, and XJ) to test the hypothesis that variety-specific metabolomic configurations systematically govern phenotypic variations in seed color, nutritional composition, and antioxidant activity. Seed color parameters, proximate components (protein, soluble sugar, starch, and total dietary fiber), and antioxidant activities (DPPH and FRAP) were evaluated. Untargeted UPLC-MS\/MS-based metabolomics was employed to characterize global metabolic profiles, screen differential metabolites, and perform KEGG pathway enrichment analysis, followed by TCMSP-based identification of bioactive compounds. JD exhibited the darkest seed color and the strongest antioxidant activity, whereas XJ showed the highest crude protein and total dietary fiber contents. Metabolomic profiling revealed that phenolic acids and flavonoids were predominantly up-regulated in JD, with coordinated down-regulation of multiple lipid species. KEGG enrichment indicated that JD\u2019s antioxidant superiority was associated with activation of the flavonoid biosynthesis pathway and suppression of carotenoid biosynthesis. Ten JD-specific bioactive compounds were identified through TCMSP screening, among which gallic acid and licochalcone B exhibited strong DPPH radical scavenging and lipid peroxidation inhibitory activities, providing a material basis for the superior antioxidant capacity of JD. These findings demonstrate that variety-specific metabolomic configurations serve as the intrinsic basis for quality differentiation in faba bean and provide metabolite-level markers for breeding programs targeting enhanced antioxidant capacity and functional quality.<\/jats:p>","DOI":"10.3390\/plants15182848","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:05:53Z","timestamp":1789988753000},"page":"2848","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Variety-Specific Metabolomic Configurations Underlie Differences in Seed Color, Nutritional Quality, and Antioxidant Activity of Faba Bean (Vicia faba L.)"],"prefix":"10.3390","volume":"15","author":[{"given":"Xueqian","family":"Niu","sequence":"first","affiliation":[{"name":"College of Agronomy, Shanxi Agricultural University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Chengda","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shanxi Agricultural University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"given":"Chengcheng","family":"Pei","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shanxi Agricultural University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Di","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shanxi Agricultural University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Xin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shanxi Institute of Organic Dryland Farming, Shanxi Agricultural University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Agronomy, Shanxi Agricultural University, Taiyuan 030031, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"ref_1","first-page":"e129","article-title":"A review of nutritional profile and processing of faba bean (Vicia faba L.)","volume":"3","author":"Dhull","year":"2021","journal-title":"Legum. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4354","DOI":"10.1080\/10408398.2024.2387330","article-title":"Exploring faba beans (Vicia faba L.): Bioactive compounds, cardiovascular health, and processing insights","volume":"65","author":"Feng","year":"2025","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Badjona, A., Bradshaw, R., Millman, C., Howarth, H., and Dubey, B. (2023). Faba Bean Processing: Thermal and Non-Thermal Processing on Chemical, Antinutritional Factors, and Pharmacological Properties. Molecules, 28.","DOI":"10.3390\/molecules28145431"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/sfp2.1028","article-title":"Current review of faba bean protein fractionation and its value-added utilization in foods","volume":"2","author":"Stone","year":"2024","journal-title":"Sustain. Food Proteins"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Dangi, P., Chaudhary, N., Paul, A., Prabha, S., Kumar, R., and Poonia, A. (2022). Faba Bean Proteins: Extraction Methods, Properties and Applications. Faba Bean: Chemistry, Properties and Functionality, Springer.","DOI":"10.1007\/978-3-031-14587-2_10"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Fantasma, F., D\u2019Urso, G., Capuano, A., Colarusso, E., Aliberti, M., Grassi, F., Brunese, M.C., Saviano, G., Felice, V.D., and Lauro, G. (2026). Potential Nutraceutical Properties of Vicia faba L: LC-ESI-HR-MS\/MS-Based Profiling of Ancient Faba Bean Varieties and Their Biological Activity. Molecules, 31.","DOI":"10.3390\/molecules31010184"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Li, Y., Wang, Z.H., Mei, C.K., Sun, W.Q., Yuan, X.X., Wang, J., and Huang, W.Y. (2025). Phytochemical Profiling, Antioxidant Capacity, and \u03b1-Amylase\/\u03b1-Glucosidase Inhibitory Effects of 29 Faba Bean (Vicia faba L.) Varieties from China. Biology, 14.","DOI":"10.3390\/biology14080982"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e70931","DOI":"10.1002\/fsn3.70931","article-title":"Comprehensive Assessment of Biochemical Traits in Diverse Faba Bean (Vicia faba L.) Germplasm for Selection of Superior Accessions","volume":"13","author":"Desta","year":"2025","journal-title":"Food Sci. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s00217-026-05056-1","article-title":"Comparison on the nutritional and functional components, and antioxidant activities of different faba bean varieties","volume":"252","author":"Wang","year":"2026","journal-title":"Eur. Food Res. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Avramidou, E., Sarri, E., Ganopoulos, I., Madesis, P., Kougiteas, L., Papadopoulou, E.A., Aliferis, K.A., Abraham, E.M., and Tani, E. (2023). Genetic and Metabolite Variability among Commercial Varieties and Advanced Lines of Vicia faba L.. Plants, 12.","DOI":"10.3390\/plants12040908"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"233","DOI":"10.4236\/ajac.2019.106018","article-title":"A comparison study of phenolic contents and in vitro antioxidant activities of Australian grown faba beans (Vicia faba L.) varying in seed coat colours as affected by extraction solvents","volume":"10","author":"Siah","year":"2019","journal-title":"Am. J. Anal. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7530","DOI":"10.1021\/acs.jafc.9b07866","article-title":"Do Faba Bean Genotypes Carrying Different Zero-Tannin Genes (zt1 and zt2) Differ in Phenolic Profiles?","volume":"68","author":"Zanotto","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"14299","DOI":"10.1038\/s41598-020-71070-2","article-title":"The bHLH transcription factor VfTT8 underlies zt2, the locus determining zero tannin content in faba bean (Vicia faba L.)","volume":"10","author":"Gutierrez","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"16174","DOI":"10.1038\/s41598-019-52575-x","article-title":"Characterization and diagnostic marker for TTG1 regulating tannin and anthocyanin biosynthesis in faba bean","volume":"9","author":"Gutierrez","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Katarzyna, G., Ewa, P., and Joanna, H. (2025). Tempeh and Fermentation-Innovative substrates in a classical microbial process. Appl. Sci., 15.","DOI":"10.3390\/app15168888"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s42779-020-00073-1","article-title":"Symbolic meaning and use of broad beans in traditional foods of the Mediterranean Basin and the Middle East","volume":"7","author":"Pasqualone","year":"2020","journal-title":"J. Ethn. Foods"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Choi, Y.M., Yoon, H., Shin, M.J., Lee, S., Yi, J., Jeon, Y.A., Wang, X.H., and Desta, K.T. (2023). Nutrient Levels, Bioactive Metabolite Contents, and Antioxidant Capacities of Faba Beans as Affected by Dehulling. Foods, 12.","DOI":"10.3390\/foods12224063"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2311","DOI":"10.1080\/10408398.2025.2568600","article-title":"Influence of Genetic Diversity and Environmental Factors on Protein Composition and Anti-Nutrient Components in Faba Bean","volume":"66","author":"Cormack","year":"2026","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1016\/j.lwt.2009.05.013","article-title":"Faba bean (Vicia faba L.) seeds darken rapidly and phenolic content falls when stored at higher temperature, moisture and light intensity","volume":"42","author":"Siddique","year":"2009","journal-title":"LWT"},{"key":"ref_20","first-page":"100800","article-title":"Mass spectrometry-based metabolites profiling of nutrients and anti-nutrients in major legume sprouts","volume":"38","author":"Farag","year":"2020","journal-title":"Food Biosci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"136491","DOI":"10.1016\/j.foodchem.2023.136491","article-title":"How does maturity stage affect seeds metabolome via UPLC\/MS based molecular networking and chemometrics and in relation to antioxidant effect? A case study in 4 major cereals and legumes","volume":"426","author":"Saied","year":"2023","journal-title":"Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Shi, S.H., Lee, S.S., Zhu, Y.M., Jin, Z.Q., Wu, F.B., and Qiu, C.W. (2022). Comparative metabolomic profiling reveals key secondary metabolites associated with high quality and nutritional value in broad bean (Vicia faba L.). Molecules, 27.","DOI":"10.3390\/molecules27248995"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Karolkowski, A., Meudec, E., Brugui\u00e8re, A., Mitaine-Offer, A.-C., Bouzidi, E., Levavasseur, L., Sommier, N., Briand, L., and Salles, C. (2023). Faba bean (Vicia faba L. minor) bitterness: An untargeted metabolomic approach to highlight the impact of the non-volatile fraction. Metabolites, 13.","DOI":"10.3390\/metabo13080964"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"100087","DOI":"10.1016\/j.fochms.2022.100087","article-title":"Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from ghana using two distinct and complementary analytical platforms","volume":"4","author":"Baxter","year":"2022","journal-title":"Food Chem. Mol. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Salma, H., Sylvain, L., Renaut, J., C\u00e9dric, J., and Kjell, S. (2026). Species-specific metabolic identities persist in Fabaceae callus cultures under standardized culture conditions. Plants, 15.","DOI":"10.3390\/plants15152299"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1007\/s11306-023-02014-w","article-title":"A comparative metabolomics investigation of flavonoid variation in faba bean flowers","volume":"19","author":"Elessawy","year":"2023","journal-title":"Metabolomics"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Yonekura-Sakakibara, K., Higashi, Y., and Nakabayashi, R. (2019). The origin and evolution of plant flavonoid metabolism. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.00943"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1758-2946-6-13","article-title":"TCMSP: A database of systems pharmacology for drug discovery from herbal medicines","volume":"6","author":"Ru","year":"2014","journal-title":"J. Cheminform."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"19637","DOI":"10.1038\/s41598-024-68743-7","article-title":"Metabolites profiling, in-vitro and molecular docking studies of five legume seeds for alzheimer\u2019s disease","volume":"14","author":"Ibrahim","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_30","unstructured":"(2016). National Food Safety Standard\u2014Determination of Protein in Foods (Standard No. GB 5009.5-2016)."},{"key":"ref_31","unstructured":"(2016). National Food Safety Standard\u2014Determination of Starch in Foods (Standard No. GB 5009.9-2016)."},{"key":"ref_32","unstructured":"(2015). National Food Safety Standard\u2014Determination of Dietary Fiber in Foods (Standard No. GB 5009.88-2014)."},{"key":"ref_33","unstructured":"(2019). Inspection of Grain and Oils\u2014Determination of Soluble Sugar in Cereals and Pulses\u2014Copper Reduction-Iodometric Titration Method (Standard No. GB\/T 37493-2019)."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0023-6438(95)80008-5","article-title":"Use of a free radical method to evaluate antioxidant activity","volume":"28","author":"Cuvelier","year":"1995","journal-title":"LWT\u2014Food Sci. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The ferric reducing ability of plasma (FRAP) as a measure of \u201cantioxidant power\u201d: The FRAP assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1016\/j.foodres.2018.09.025","article-title":"Unravelling the phytonutrients and antioxidant properties of European Vicia faba L. seeds","volume":"116","author":"Valente","year":"2019","journal-title":"Food Res. Int."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.foodchem.2013.07.068","article-title":"Effects of soaking, boiling and autoclaving on the phenolic contents and antioxidant activities of faba beans (Vicia faba L.) differing in seed coat colours","volume":"142","author":"Siah","year":"2014","journal-title":"Food Chem."},{"key":"ref_38","first-page":"36","article-title":"Theoretical calculation on antioxidant activity of phenolic acids","volume":"32","author":"Chen","year":"2011","journal-title":"Food Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.foodchem.2013.03.089","article-title":"Antioxidant and anti-inflammatory activities of six flavonoids separated from licorice","volume":"141","author":"Fu","year":"2013","journal-title":"Food Chem."},{"key":"ref_40","first-page":"1342","article-title":"Antioxidative and superoxide scavenging activities of retrochalcones in Glycyrrhiza inflata","volume":"24","author":"Haraguchi","year":"1998","journal-title":"Free Radic. Biol. Med."},{"key":"ref_41","first-page":"356","article-title":"Research progress on pharmacological effects of licochalcone B","volume":"33","author":"Wang","year":"2015","journal-title":"J. Shihezi Univ. (Nat. Sci.)"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Qu, L., Wu, J., Tang, Y., Cao, Y., Li, Y., Wang, N., and Liu, S. (2022). Licochalcone B, a natural autophagic agent for alleviating oxidative stress-induced cell death in neuronal cells and Caenorhabditis elegans models. Pharmaceuticals, 15.","DOI":"10.3390\/ph15091052"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Sergeant, K., Goertz, S., Halime, S., Tietgen, H., Heidt, H., Minestrini, M., Jacquard, C., Zimmer, S., and Renaut, J. (2024). Exploration of the Diversity of Vicine and Convicine Derivatives in Faba Bean (Vicia faba L.) Cultivars: Insights from LC-MS\/MS Spectra. Molecules, 29.","DOI":"10.3390\/molecules29051065"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"136101","DOI":"10.1016\/j.molstruc.2023.136101","article-title":"Explore the effect of the structure-activity relationship and dose-effect relationship on the antioxidant activity of licorice flavonoids","volume":"1295","author":"Hu","year":"2023","journal-title":"J. Mol. Struct."},{"key":"ref_45","first-page":"1445","article-title":"Evaluation of Antioxidant Interactions of Combined Model Systems of Phenolics in the Presence of Sugars","volume":"11","author":"Kopjar","year":"2016","journal-title":"Nat. Prod. Commun."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/18\/2848\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:19:39Z","timestamp":1790050779000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/18\/2848"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,17]]},"references-count":45,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["plants15182848"],"URL":"https:\/\/doi.org\/10.3390\/plants15182848","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,17]]}}}