{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:17:05Z","timestamp":1789993025787,"version":"4.0.1"},"reference-count":53,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:00:00Z","timestamp":1789776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The restricted genetic diversity of current cotton varieties (Gossypium hirsutum L.) considerably limits breeding opportunities aimed at enhancing fiber quality and resistance to environmental factors. Wild types of allotetraploid cottons are valuable sources of genetic variation, and their breeding potential has not yet been fully utilized. In this study, the inheritance and variability of important agronomic traits were analyzed in F1 and F2 hybrid generations obtained from intra- and interspecific crosses involving G. hirsutum, G. barbadense, and G. darwinii. The phylogenetic relationships of allotetraploid species were analyzed using molecular-genetic and cytogenetic methods, and their breeding potential was evaluated. Cytogenetic analysis of the F1 hybrids revealed the occurrence of univalents at metaphase I of meiosis, while bivalent numbers reached 25.9 in some hybrid combinations. Based on molecular marker analysis, significant genetic differences and polymorphism were identified between var. gambia and var. m. palmerii. There were reciprocal translocations, shown as multivalents, during ecotypic differentiation in other cross combinations. Positive transgressive segregation for the enhancement of fiber length was identified in F2 populations. Notably, in certain hybrid combinations, fiber length extended to 40.9 mm. Furthermore, molecular analysis identified 113 unique alleles across the genotypes examined. These findings affirm that wild and exotic allotetraploid cotton forms constitute a significant source of genetic diversity for breeding programs.<\/jats:p>","DOI":"10.3390\/plants15182866","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:05:53Z","timestamp":1789988753000},"page":"2866","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Dynamics of Allotetraploid Cotton Species (Gossypium spp.) and Their Utilization in Breeding for Superior Fiber Quality"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0434-7952","authenticated-orcid":false,"given":"Dilrabo K.","family":"Ernazarova","sequence":"first","affiliation":[{"name":"Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent 111208, Uzbekistan"},{"name":"Department of Genetics, Institute of Biochemistry, Samarkand 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(1954). Khlopchatnik (Cotton), Academy of Sciences of the Uzbek SSR."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Fang, D.D., and Percy, R.G. (2015). Taxonomy and evolution of the cotton genus, Gossypium. Cotton, American Society of Agronomy. Agronomy Monographs.","DOI":"10.2134\/agronmonogr57"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1038\/nbt.3208","article-title":"Genome sequence of cultivated upland cotton (Gossypium hirsutum TM-1) provides insights into genome evolution","volume":"33","author":"Li","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_4","first-page":"36","article-title":"On the nomenclature of the genus Gossypium L.","volume":"6","author":"Abdullaev","year":"2006","journal-title":"Uzb. Biol. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1139\/cjb-2012-0192","article-title":"Genetic diversity and population structure of cotton (Gossypium spp.) of the New World assessed by SSR markers","volume":"91","author":"Ulloa","year":"2013","journal-title":"Botany"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"108","DOI":"10.22244\/rheedea.1992.02.02.04","article-title":"A revised taxonomic interpretation of Gossypium L. (Malvaceae)","volume":"2","author":"Fryxell","year":"1992","journal-title":"Rheedea"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0065-2113(02)78004-8","article-title":"Polyploidy and the evolutionary history of cotton","volume":"78","author":"Wendel","year":"2003","journal-title":"Adv. Agron."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s10722-014-0138-x","article-title":"Molecular confirmation of species status for the allopolyploid cotton species, Gossypium ekmanianum Wittmack","volume":"62","author":"Grover","year":"2015","journal-title":"Genet. Resour. Crop Evol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1093\/molbev\/msg065","article-title":"Rate variation among nuclear genes and the age of polyploidy in Gossypium","volume":"20","author":"Senchina","year":"2003","journal-title":"Mol. Biol. Evol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Paterson, A.H. (2009). Evolution and natural history of the cotton genus. Genetics and Genomics of Cotton, Springer.","DOI":"10.1007\/978-0-387-70810-2"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e2607107123","DOI":"10.1073\/pnas.2607107123","article-title":"Genomic diversity and the domestication history of cotton (Gossypium hirsutum)","volume":"123","author":"Ning","year":"2026","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2620840","DOI":"10.1080\/23311932.2026.2620840","article-title":"Phylogenetic relationships of tetraploid cotton species (Gossypium subsp.) and their genetic potential for breeding programs","volume":"12","author":"Rafieva","year":"2026","journal-title":"Cogent Food Agric."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1007\/PL00002908","article-title":"A genetic bottleneck in the evolution under domestication of upland cotton (Gossypium hirsutum L.) examined using DNA fingerprinting","volume":"103","author":"Iqbal","year":"2001","journal-title":"Theor. Appl. Genet."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1600\/036364417X694593","article-title":"A new species of cotton from Wake Atoll, Gossypium stephensii (Malvaceae)","volume":"42","author":"Gallagher","year":"2017","journal-title":"Syst. Bot."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"607091","DOI":"10.1155\/2014\/607091","article-title":"Molecular Markers and Cotton Genetic Improvement: Current Status and Future Prospects","volume":"2014","author":"Malik","year":"2014","journal-title":"Sci. World J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2589531","DOI":"10.1080\/17429145.2025.2589531","article-title":"Genetic improvement of cultivated cotton (Gossypium hirsutum) for drought and pest resistance through hybridization with wild species","volume":"20","author":"Kholova","year":"2025","journal-title":"J. Plant Interact."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Arslanova, S.K., Ernazarova, D.K., Ernazarova, D.K., Turaev, O.S., Safiullina, A.K., Toshpulatov, A.K., Kholova, M.D., Azimova, L.A., Rafiyeva, F.U., and Gapparov, B.M. (2025). Development and characterization of synthetic allotetraploids between diploid species Gossypium herbaceum and Gossypium nelsonii for cotton genetic improvement. Plants, 14.","DOI":"10.3390\/plants14111620"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Tufail, M.B., Yasir, M., Zuo, D., Cheng, H., Ali, M., Hafeez, A., Soomro, M., and Song, G. (2023). Identification and Characterization of Phytocyanin Family Genes in Cotton Genomes. Genes, 14.","DOI":"10.3390\/genes14030611"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s10681-005-4629-x","article-title":"Construction of a genetic linkage map and QTL analysis of fibre-related traits in upland cotton","volume":"144","author":"Zhang","year":"2005","journal-title":"Euphytica"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2686078","DOI":"10.1080\/17429145.2026.2686078","article-title":"Development of high fiber quality, high-yielding, and salt stress-tolerant initial cotton genotypes using gene pyramiding technology","volume":"21","author":"Azimova","year":"2026","journal-title":"J. Plant Interact."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Toshpulatov, A.K., Turaev, O.S., Iskandarov, A.A., Khalikov, K.K., Arslanova, S.K., Safiullina, A.K., Kudratova, M.K., Oripova, B.B., Rafieva, F.U., and Kholova, M.D. (2025). Identification of stable meta-QTLs and candidate genes underlying fiber quality and agronomic traits in cotton. Plants, 14.","DOI":"10.3390\/plants14213252"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Ernazarova, D.K., Safiullina, A.K., Kholova, M.D., Azimova, L.A., Hasanova, S.A., Nematullaeva, E.F., Rafieva, F.U., Akhmedova, N.S., Khursandova, M.S., and Turaev, O.S. (2025). Morphological and molecular insights into genetic variability and heritability in four strawberry (Fragaria \u00d7 ananassa) cultivars. Horticulturae, 11.","DOI":"10.3390\/horticulturae11101195"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2546997","DOI":"10.1080\/17429145.2025.2546997","article-title":"Molecular characterization of heat tolerance in bread wheat (Triticum aestivum L.) cultivars\/lines using genomic SSR markers","volume":"20","author":"Erjigitov","year":"2025","journal-title":"J. Plant Interact."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.54910\/sabrao2025.57.3.16","article-title":"Assessment of heat tolerance in wheat (Triticum aestivum L.) at the seedling stage","volume":"57","author":"Mamatkulova","year":"2025","journal-title":"SABRAO J. Breed. Genet."},{"key":"ref_25","first-page":"319","article-title":"Estimation of gene effects for some quantitative characters in upland cotton","volume":"38","author":"Singh","year":"1983","journal-title":"Cotton Fibres Trop."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"988","DOI":"10.54910\/sabrao2024.56.3.8","article-title":"Bibliometric analysis of the past research based on MAS technology in cotton improvement","volume":"56","author":"Umedova","year":"2024","journal-title":"SABRAO J. Breed. Genet."},{"key":"ref_27","unstructured":"Avtonomov, V.A. (2010). Vnutrividovaya Geograficheski Otdalennaya Gibridizatsiya Khlopchatnika na Ustoychivost\u2019 k vil\u2019tu i Chernoy Kornevoy Gnili. [Doctoral Dissertation, Uzbek Scientific Research Institute of Cotton Selection and Seed Production]. (In Russian)."},{"key":"ref_28","unstructured":"Avtonomov, V.A. (2006). Geograficheski Otdalennaya Gibridizatsiya V Selektsii Srednevoloknistykh Sortov Khlopchatnika, Turon-Iqbol. (In Russian)."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1051\/agro:2007041","article-title":"Genotypic variation for drought tolerance in cotton","volume":"28","author":"Rahman","year":"2008","journal-title":"Agron. Sustain. Dev."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Li, C., Yu, H., Li, C., Zhao, T., Dong, Y., Deng, X., Hu, J., Zhang, Y., Zhang, F., and Daud, M.K. (2018). QTL mapping and heterosis analysis for fiber quality traits across multiple genetic populations and environments in upland cotton. Front. Plant Sci., 9.","DOI":"10.3389\/fpls.2018.01364"},{"key":"ref_31","unstructured":"Efimenko, V.M. (1960). Vyhod volokna khlopchatnika i nekotorye puti ego povysheniya. Voprosy Genetiki, Selektsii i Semenovodstva Khlopchatnika, Fan. (In Russian)."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Zhu, T., Liang, C., Meng, Z., Sun, G., Meng, Z., Guo, S., and Zhang, R. (2017). CottonFGD: An integrated functional genomics database for cotton. BMC Plant Biol., 17.","DOI":"10.1186\/s12870-017-1039-x"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1023\/A:1019951120788","article-title":"The proline-rich, extensin-like receptor kinase-1 (PERK1) gene is rapidly induced by wounding","volume":"50","author":"Silva","year":"2002","journal-title":"Plant Mol. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1093\/aob\/mct298","article-title":"GPT2: A glucose 6-phosphate\/phosphate translocator with a novel role in the regulation of sugar signalling during seedling development","volume":"113","author":"Dyson","year":"2014","journal-title":"Ann. Bot."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Kushanov, F., Turaev, O., Ernazarova, D., Gapparov, B., Oripova, B., Kudratova, M., Rafieva, F., Khalikov, K., Erjigitov, D., and Khidirov, M. (2021). Genetic diversity, QTL mapping, and marker-assisted selection technology in cotton (Gossypium spp.). Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.779386"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Kushanov, F.N., Komilov, D.J., Turaev, O.S., Ernazarova, D.K., Amanboyeva, R.S., Gapparov, B.M., and Yu, J.Z. (2022). Genetic analysis of mutagenesis that induces the photoperiod insensitivity of wild cotton Gossypium hirsutum subsp. purpurascens. Plants, 11.","DOI":"10.3390\/plants11223012"},{"key":"ref_37","first-page":"35","article-title":"Inheritance of quantitative characters in sorghum","volume":"3","author":"Beil","year":"1965","journal-title":"Iowa State J. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"427","DOI":"10.2134\/agronj1952.00021962004400080007x","article-title":"A Method for Estimating Heritability","volume":"44","author":"Warner","year":"1952","journal-title":"Agron. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1002\/j.1537-2197.1992.tb13734.x","article-title":"Genetic diversity in Gossypium hirsutum and the origin of upland cotton","volume":"79","author":"Wendel","year":"1992","journal-title":"Am. J. Bot."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e70239","DOI":"10.1111\/mec.70239","article-title":"Comparative population genomics of relictual Caribbean island Gossypium hirsutum","volume":"35","author":"Ning","year":"2026","journal-title":"Mol. Ecol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Coppens d\u2019Eeckenbrugge, G., and Lacape, J.-M. (2014). Distribution and differentiation of wild, feral, and cultivated populations of perennial upland cotton (Gossypium hirsutum L.) in Mesoamerica and the Caribbean. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0107458"},{"key":"ref_42","unstructured":"OriginLab Corporation OriginPro 2021, OriginLab Corporation. Available online: https:\/\/www.originlab.com."},{"key":"ref_43","unstructured":"Moore, D.S., and McCabe, G.P. (2003). Introduction to the Practice of Statistics, W.H. Freeman. [4th ed.]."},{"key":"ref_44","unstructured":"Lemeshev, N., Atlanov, A., Podolnaya, L., and Korneichuk, V. (1989). Wide Unified CMEA Classifier of the Genus Gossypium L., VIR."},{"key":"ref_45","unstructured":"Pausheva, Z.P. (1988). Practical Course in Cytology, Kolos."},{"key":"ref_46","unstructured":"Barykina, R.P., Veselova, T.D., Devyatov, A.G., Dzhalilova, K.K., Il\u2019ina, G.M., and Chubatova, N.V. (2000). Osnovy Mikrotekhnicheskikh Issledovaniy Botaniki, Spravochnoye Rukovodstvo. (In Russian)."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1007\/BF02670470","article-title":"A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis","volume":"11","author":"Paterson","year":"1993","journal-title":"Plant Mol. Biol. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"iyaf243","DOI":"10.1093\/genetics\/iyaf243","article-title":"CottonGen 2025: A knowledgebase for cotton genomics, genetics, and breeding research","volume":"232","author":"Yu","year":"2026","journal-title":"Genetics"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1093\/bioinformatics\/bts091","article-title":"Unipro UGENE: A unified bioinformatics toolkit","volume":"28","author":"Okonechnikov","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Stanke, M., Sch\u00f6ffmann, O., Morgenstern, B., and Waack, S. (2006). Gene prediction in eukaryotes with a generalized hidden Markov model that uses hints from external sources. BMC Bioinform., 7.","DOI":"10.1186\/1471-2105-7-62"},{"key":"ref_51","first-page":"403","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"Bioinformatics"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_53","unstructured":"Dospekhov, B.A. (1985). Methodology of Field Experiments, Agropromizdat."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/18\/2866\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:52:16Z","timestamp":1789991536000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/18\/2866"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,19]]},"references-count":53,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["plants15182866"],"URL":"https:\/\/doi.org\/10.3390\/plants15182866","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,19]]}}}