{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:10:21Z","timestamp":1792789821127,"version":"4.5.0"},"reference-count":73,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Jilin Province Science & Technology","award":["YDZJ202501ZYTS514"],"award-info":[{"award-number":["YDZJ202501ZYTS514"]}]},{"name":"Education Department of Jilin Province","award":["JJKH20262370CY"],"award-info":[{"award-number":["JJKH20262370CY"]}]},{"name":"Foundation of Ph.D. Research Project of Jilin Medical University","award":["JYBS2023006LK"],"award-info":[{"award-number":["JYBS2023006LK"]}],"id":[{"id":"https:\/\/ror.org\/03mzw7781","id-type":"ROR","asserted-by":"crossref"}]},{"award":["JYBS2023006LK"],"award-info":[{"award-number":["JYBS2023006LK"]}],"id":[{"id":"https:\/\/ror.org\/03mzw7781","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Jilin Students\u2019 Platform for innovation and entrepreneurship training program","award":["S202513706085"],"award-info":[{"award-number":["S202513706085"]}]},{"name":"Jilin Students\u2019 Platform for innovation and entrepreneurship training program","award":["S202513706108X"],"award-info":[{"award-number":["S202513706108X"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The western part of north-eastern China is mostly covered by soda soil, which has a significant impact on the growth, breeding and production of crops. The present study investigates the physiological responses and metabolic adaptation of Chlamydomonas reinhardtii P.A.Dangeard (C. reinhardtii) under different types of alkaline stress and the potential application to soda soil improvement and plants in response to alkaline stress. The soda\u2013saline soil was treated with C. reinhardtii for 5 and 120 days, respectively, and the chemical properties of the soil were determined. The microalga was cultured for a period of 12 days, with and without an alkaline medium, and then the metabolic profiles of the microalgae were then analyzed by metabolomics. Results showed that C. reinhardtii exhibited a high level of tolerance to 40 mM NaHCO3, pH 8.3, and pH 10.3 with an increase in incubation time. Moreover, C. reinhardtii has been demonstrated to ameliorate selected physicochemical properties of soda\u2013saline soil by reducing soil pH, electrical conductivity (EC), and the contents of CO32\u2212 and HCO3\u2212 by 40\u2013100% (w\/w, wet biomass) addition for 120 cultivation days, respectively. It was demonstrated that rice seedlings were less affected by alkaline stress in the presence of C. reinhardtii. Furthermore, C. reinhardtii has the capacity to adapt to alkaline environments by regulating key pathways such as cofactor biosynthesis, glycerophospholipid metabolism, amino acid biosynthesis, purine metabolism, carbon metabolism, tropane, piperidine, and pyridine alkaloid biosynthesis. This adaptation is characterized by the accumulation of metabolites, including saccharides, amino acids and organic acids. These interact with high pH, CO32\u2212 and HCO3\u2212 ions. This study elucidates that C. reinhardtii has the capacity to improve soda\u2013saline soil and respond well to diverse alkaline stresses, attributable to its intricate metabolic profile.<\/jats:p>","DOI":"10.3390\/plants15182879","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:05:53Z","timestamp":1789988753000},"page":"2879","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolic Profile of Chlamydomonas reinhardtii in Alkaline Environment and Role in Sodic Soil Improvement and Rice Seedlings in Response to Alkaline Stress"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3209-1616","authenticated-orcid":false,"given":"Dan","family":"Wang","sequence":"first","affiliation":[{"name":"School of Public Health, Jilin Medical University, Jilin 132013, China"},{"name":"Microalgae Resource Development and Application Research Center, Jilin Medical University, Jilin 132013, China"},{"name":"Jilin Provincial School-Enterprise Cooperation Innovative Laboratory of Food Nutrition and Health and Functional Food Innovation, Jilin Medical University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Yunqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin Medical University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Lan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin Medical University, Jilin 132013, China"},{"name":"Microalgae Resource Development and Application Research Center, Jilin Medical University, Jilin 132013, China"},{"name":"Jilin Provincial School-Enterprise Cooperation Innovative Laboratory of Food Nutrition and Health and Functional Food Innovation, Jilin Medical University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9434-9566","authenticated-orcid":false,"given":"Yanli","family":"Xi","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin Medical University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Chen","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin Medical University, Jilin 132013, China"},{"name":"Microalgae Resource Development and Application Research Center, Jilin Medical University, Jilin 132013, China"},{"name":"Jilin Provincial School-Enterprise Cooperation Innovative Laboratory of Food Nutrition and Health and Functional Food Innovation, Jilin Medical University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Xiaoyu","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin Medical University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Min","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin Medical University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Yifan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin Medical University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Jiajun","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Public Health, Jilin Medical University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"47867","DOI":"10.1007\/s11356-022-20489-3","article-title":"Mechanisms of straw biochar\u2019s improvement of phosphorus bioavailability in soda saline-alkali soil","volume":"29","author":"Li","year":"2022","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_2","unstructured":"FAO (2024). Global Status of Salt-Affected Soils, FAO."},{"key":"ref_3","unstructured":"Choosing People\u2019s Daily Online (2023, November 17). Saline-Alkali Land Transformed into Fertile Soil in NE China\u2019s Jilin. Available online: https:\/\/en.people.cn\/n3\/2023\/1117\/c98649-20098972.html."},{"key":"ref_4","unstructured":"Xu, B. (2025). Saline-Alkali Land. Contextual Dictionary of Chinese Cultural Knowledge, Springer Nature."},{"key":"ref_5","unstructured":"Eric Ruto, D.T., Gould, I., and Bosworth, G. (2021). Future of Sustainable Agriculture in Saline Environments, CRC Press."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Yang, S., Xu, Y., Tang, Z., Jin, S., and Yang, S. (2024). The Impact of Alkaline Stress on Plant Growth and Its Alkaline Resistance Mechanisms. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252413719"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"103155","DOI":"10.1016\/j.algal.2023.103155","article-title":"Salinity stress provokes diverse physiological responses of eukaryotic unicellular microalgae","volume":"73","author":"Farkas","year":"2023","journal-title":"Algal Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"105543","DOI":"10.1016\/j.envexpbot.2023.105543","article-title":"Evidence of physiological adaptation of Chlorella vulgaris under extreme salinity\u2013new insights into a potential halotolerance strategy","volume":"216","author":"Mountourakis","year":"2023","journal-title":"Environ. Exp. Bot."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1007\/s00449-020-02293-w","article-title":"Enhanced lipid productivity of Chlamydomonas reinhardtii with combination of NaCl and CaCl(2) stresses","volume":"43","author":"Hang","year":"2020","journal-title":"Bioprocess. Biosyst. Eng."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1186\/s13068-021-01970-6","article-title":"Quantitative proteomic comparison of salt stress in Chlamydomonas reinhardtii and the snow alga Chlamydomonas nivalis reveals mechanisms for salt-triggered fatty acid accumulation via reallocation of carbon resources","volume":"14","author":"Hounslow","year":"2021","journal-title":"Biotechnol. Biofuels"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/S0005-2728(89)80347-0","article-title":"Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: Verification of the concentration of chlorophyll standards by atomic absorption spectroscopy","volume":"975","author":"Porra","year":"1989","journal-title":"Biochim. Biophys. Acta Bioenerg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1093\/plcell\/koab293","article-title":"The Chlamydomonas bZIP transcription factor BLZ8 confers oxidative stress tolerance by inducing the carbon-concentrating mechanism","volume":"34","author":"Choi","year":"2022","journal-title":"Plant Cell"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ecoenv.2017.05.048","article-title":"Sensitivity of two green microalgae to copper stress: Growth, oxidative and antioxidants analyses","volume":"144","author":"Hamed","year":"2017","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e14233","DOI":"10.1111\/ppl.14233","article-title":"Combined high light and salt stress enhances accumulation of PsbS and zeaxanthin in Chlamydomonas reinhardtii","volume":"176","author":"Hemker","year":"2024","journal-title":"Physiol. Plant"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"144596","DOI":"10.1016\/j.foodchem.2025.144596","article-title":"Enhanced lipid production in Chlamydomonas reinhardtii: Impact of salt stress synergized with \u03b1-lipoic acid","volume":"486","author":"Xing","year":"2025","journal-title":"Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Elloumi, W., Jebali, A., Maalej, A., Chamkha, M., and Sayadi, S. (2020). Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae Scenedesmus sp.. Biomolecules, 10.","DOI":"10.3390\/biom10111515"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"De Jaeger, L., Carreres, B.M., Springer, J., Schaap, P.J., Eggink, G., Martins Dos Santos, V.A.P., Wijffels, R.H., and Martens, D.E. (2018). Neochloris oleoabundans is worth its salt: Transcriptomic analysis under salt and nitrogen stress. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0194834"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"101136","DOI":"10.1016\/j.redox.2019.101136","article-title":"A concise appraisal of lipid oxidation and lipoxidation in higher plants","volume":"23","year":"2019","journal-title":"Redox Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1007\/s11104-025-07810-y","article-title":"Mechanisms of salt tolerance: Insights into photosystem function and carbon metabolism in tomato seedlings","volume":"516","author":"Li","year":"2025","journal-title":"Plant Soil"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jphotobiol.2013.04.007","article-title":"Alteration of photochemistry and protein degradation of photosystem II from Chlamydomonas reinhardtii under high salt grown cells","volume":"124","author":"Neelam","year":"2013","journal-title":"J. Photochem. Photobiol. B"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1007\/s11120-025-01179-w","article-title":"Investigating the role of PGR5 and PGRL1 in photosynthetic acclimation to salt stress in Chlamydomonas reinhardtii","volume":"163","author":"Damaraju","year":"2025","journal-title":"Photosynth. Res."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Devadasu, E., Kanna, S.D., Neelam, S., Yadav, R.M., Nama, S., Akhtar, P., Polg\u00e1r, T.F., Ughy, B., Garab, G., and Lambrev, P.H. (2023). Long- and short-term acclimation of the photosynthetic apparatus to salinity in Chlamydomonas reinhardtii. The role of Stt7 protein kinase. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1051711"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"137409","DOI":"10.1016\/j.chemosphere.2022.137409","article-title":"Improved effects of combined application of nitrogen-fixing bacteria Azotobacter beijerinckii and microalgae Chlorella pyrenoidosa on wheat growth and saline-alkali soil quality","volume":"313","author":"Zhou","year":"2023","journal-title":"Chemosphere"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Song, J., Guan, X., Cui, H., Liu, L., Li, Y., Li, Y., and Ma, S. (2025). The impact of salt-tolerant plants on soil nutrients and microbial communities in soda saline-alkali lands of the Songnen plain. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1592834"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"108591","DOI":"10.1016\/j.biotechadv.2025.108591","article-title":"Bacteria-microalgae interactions from an evolutionary perspective and their biotechnological significance","volume":"82","author":"Elnagdy","year":"2025","journal-title":"Biotechnol. Adv."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1038\/s41596-024-01135-3","article-title":"Preparation, maintenance and propagation of synchronous cultures of photoactive Chlamydomonas cells","volume":"20","author":"Catalan","year":"2025","journal-title":"Nat. Protoc."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Atikij, T., Syaputri, Y., Iwahashi, H., Praneenararat, T., Sirisattha, S., Kageyama, H., and Waditee-Sirisattha, R. (2019). Enhanced Lipid Production and Molecular Dynamics under Salinity Stress in Green Microalga Chlamydomonas reinhardtii (137C). Mar. Drugs, 17.","DOI":"10.3390\/md17080484"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ji, C., Mao, X., Hao, J., Wang, X., Xue, J., Cui, H., and Li, R. (2018). Analysis of bZIP Transcription Factor Family and Their Expressions under Salt Stress in Chlamydomonas reinhardtii. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19092800"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Wang, A., Wang, R., and Miao, X. (2025). Mechanism of Transcription Factor ChbZIP1 Enhanced Alkaline Stress Tolerance in Chlamydomonas reinhardtii. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26020769"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1073\/pnas.54.6.1665","article-title":"Cytochrome f and plastocyanin: Their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi","volume":"54","author":"Gorman","year":"1965","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_31","unstructured":"Carter, M.R., and Gregorich, E.G. (2022). Soil Sampling and Methods of Analysis, Publishing House of Electronics Industry."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Ren, J., Zhao, K., Wu, X., Zheng, X., and Li, X. (2018). Comparative Analysis of the Spectral Response to Soil Salinity of Saline-Sodic Soils under Different Surface Conditions. Int. J. Environ. Res. Public. Health, 15.","DOI":"10.3390\/ijerph15122721"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3322","DOI":"10.1021\/acs.jproteome.5b00354","article-title":"Analysis of the Human Adult Urinary Metabolome Variations with Age, Body Mass Index, and Gender by Implementing a Comprehensive Workflow for Univariate and OPLS Statistical Analyses","volume":"14","author":"Roux","year":"2015","journal-title":"J. Proteome Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.cbpa.2017.01.001","article-title":"How close are we to complete annotation of metabolomes?","volume":"36","author":"Viant","year":"2017","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e0129","DOI":"10.1199\/tab.0129","article-title":"Jasmonates","volume":"8","author":"Acosta","year":"2010","journal-title":"Arab. Book"},{"key":"ref_36","unstructured":"Liu, H.-W., and Begley, T.P. (2020). 2.22-Biosynthesis of Aminoglycoside Antibiotics. Comprehensive Natural Products III, Elsevier."},{"key":"ref_37","unstructured":"Batt, C.A., and Tortorello, M.L. (2014). METABOLIC PATHWAYS|Nitrogen Metabolism. Encyclopedia of Food Microbiology, Academic Press. [2nd ed.]."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"111238","DOI":"10.1016\/j.plantsci.2022.111238","article-title":"Glutamate: A multifunctional amino acid in plants","volume":"318","author":"Liao","year":"2022","journal-title":"Plant Sci."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Winter, G., Todd, C.D., Trovato, M., Forlani, G., and Funck, D. (2015). Physiological implications of arginine metabolism in plants. Front. Plant Sci., 6.","DOI":"10.3389\/fpls.2015.00534"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s44307-024-00022-y","article-title":"Significance of Raffinose Family Oligosaccharides (RFOs) metabolism in plants","volume":"2","author":"Liu","year":"2024","journal-title":"Adv. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Sanyal, R., Kumar, S., Pattanayak, A., Kar, A., and Bishi, S.K. (2023). Optimizing raffinose family oligosaccharides content in plants: A tightrope walk. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1134754"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1093\/pcp\/pcad071","article-title":"The Roles of Functional Amino Acids in Plant Growth and Development","volume":"64","author":"Kawade","year":"2023","journal-title":"Plant Cell Physiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"162238","DOI":"10.1016\/j.scitotenv.2023.162238","article-title":"Coproduction of amino acids and biohythane from microalgae via cascaded hydrothermal and anaerobic process","volume":"872","author":"Wu","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Ingrisano, R., Tosato, E., Trost, P., Gurrieri, L., and Sparla, F. (2023). Proline, Cysteine and Branched-Chain Amino Acids in Abiotic Stress Response of Land Plants and Microalgae. Plants, 12.","DOI":"10.3390\/plants12193410"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Oliveira, E.R., Nunes, A., Dutra, F.S., Azevedo, G.Z., Schneider, A.R., Dos Santos, B.R., Munaro, D., Moura, S., Lima, G.P.P., and Maraschin, M. (2025). Marine and terrestrial biostimulant elicitors of tolerance to cold stress. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1569516"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Tsitsekian, D., Mylona, P., Kamargiakis, E., Rigas, S., and Daras, G. (2025). Developmental and Stress-Mediated Transcriptional Shifts in Riboflavin Metabolism Pathway in Arabidopsis. Genes, 17.","DOI":"10.3390\/genes17010016"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2127","DOI":"10.1104\/pp.16.00540","article-title":"The Peroxisomal NAD Carrier from Arabidopsis Imports NAD in Exchange with AMP","volume":"171","author":"Schroers","year":"2016","journal-title":"Plant Physiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1007\/s12298-023-01349-x","article-title":"Crosstalk of AsA\/DHA and GSH\/GSSG ratios\u2019 role in growth-phase dependent antioxidative defense in euryhaline and freshwater microalgae: Explored for the first time","volume":"29","author":"Kaur","year":"2023","journal-title":"Physiol. Mol. Biol. Plants"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s11120-021-00889-1","article-title":"Plastoquinone homeostasis in plant acclimation to light intensity","volume":"152","author":"Ksas","year":"2022","journal-title":"Photosynth. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"5340","DOI":"10.1021\/bi00236a003","article-title":"Full replacement of the function of the secondary electron acceptor phylloquinone(=vitamin K1) by non-quinone carbonyl compounds in green plant photosystem I photosynthetic reaction centers","volume":"30","author":"Itoh","year":"1991","journal-title":"Biochemistry"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"19141","DOI":"10.1074\/jbc.M301808200","article-title":"Control of electron transport in photosystem I by the iron-sulfur cluster FX in response to intra- and intersubunit interactions","volume":"278","author":"Gong","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1038\/s41477-024-01780-2","article-title":"Differential FeS cluster photodamage plays a critical role in regulating excess electron flow through photosystem I","volume":"10","author":"Tiwari","year":"2024","journal-title":"Nat. Plants"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Tejada-Jimenez, M., Leon-Miranda, E., and Llamas, A. (2023). Chlamydomonas reinhardtii\u2014A Reference Microorganism for Eukaryotic Molybdenum Metabolism. Microorganisms, 11.","DOI":"10.20944\/preprints202305.1259.v1"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1111\/jpy.13083","article-title":"A marine Chlamydomonas sp. emerging as an algal model","volume":"57","author":"Fricke","year":"2021","journal-title":"J. Phycol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3834","DOI":"10.1128\/aem.62.10.3834-3839.1996","article-title":"Resistance to Phosphinothricin (Glufosinate) and Its Utilization as a Nitrogen Source by Chlamydomonas reinhardtii","volume":"62","author":"Franco","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s11306-019-1496-3","article-title":"Increased urea availability promotes adjustments in C\/N metabolism and lipid content without impacting growth in Chlamydomonas reinhardtii","volume":"15","author":"Batista","year":"2019","journal-title":"Metabolomics"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.jbiotec.2023.03.009","article-title":"Urea as a source of nitrogen and carbon leads to increased photosynthesis rates in Chlamydomonas reinhardtii under mixotrophy","volume":"367","author":"Rosa","year":"2023","journal-title":"J. Biotechnol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"111369","DOI":"10.1007\/s11356-023-30231-2","article-title":"Effect of macronutrients (carbon, nitrogen, and phosphorus) on the growth of Chlamydomonas reinhardtii and nutrient recovery under different trophic conditions","volume":"30","author":"Fletcher","year":"2023","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.algal.2015.03.023","article-title":"Discovery of two novel highly tolerant NaHCO3 Trebouxiophytes: Identification and characterization of microalgae from extreme saline\u2013alkali soil","volume":"9","author":"Qiao","year":"2015","journal-title":"Algal Res."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Youssef, S.M., El-Serafy, R.S., Ghanem, K.Z., Elhakem, A., and Abdel Aal, A.A. (2022). Foliar Spray or Soil Drench: Microalgae Application Impacts on Soil Microbiology, Morpho-Physiological and Biochemical Responses, Oil and Fatty Acid Profiles of Chia Plants under Alkaline Stress. Biology, 11.","DOI":"10.3390\/biology11121844"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s10653-023-01790-7","article-title":"Soil health improvement by inoculation of indigenous microalgae in saline soil","volume":"46","author":"Zhang","year":"2024","journal-title":"Environ. Geochem. Health"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Zayed, O., Hewedy, O.A., Abdelmoteleb, A., Ali, M., Youssef, M.S., Roumia, A.F., Seymour, D., and Yuan, Z.-C. (2023). Nitrogen Journey in Plants: From Uptake to Metabolism, Stress Response, and Microbe Interaction. Biomolecules, 13.","DOI":"10.20944\/preprints202308.1575.v1"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s11120-011-9643-3","article-title":"Carbon dioxide concentrating mechanism in Chlamydomonas reinhardtii: Inorganic carbon transport and CO2 recapture","volume":"109","author":"Wang","year":"2011","journal-title":"Photosynth. Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"3651","DOI":"10.1093\/jxb\/erad116","article-title":"The Chlamydomonas reinhardtii chloroplast envelope protein LCIA transports bicarbonate in planta","volume":"74","author":"Rourke","year":"2023","journal-title":"J. Exp. Bot."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/s41477-025-02200-9","article-title":"Structure of Chlamydomonas reinhardtii LciA guided the engineering of FNT family proteins to gain bicarbonate transport activity","volume":"12","author":"Guo","year":"2026","journal-title":"Nat. Plants"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1007\/s11104-025-07245-5","article-title":"Responses of rhizosphere microbial communities and resource competition to soil amendment in saline and alkaline soils","volume":"513","author":"Chang","year":"2025","journal-title":"Plant Soil"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Su, Y., Ren, Y., Wang, G., Li, J., Zhang, H., Yang, Y., Pang, X., and Han, J. (2024). Microalgae and microbial inoculant as partial substitutes for chemical fertilizer enhance Polygala tenuifolia yield and quality by improving soil microorganisms. Front. Plant Sci., 15.","DOI":"10.3389\/fpls.2024.1499966"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"118529","DOI":"10.1016\/j.jenvman.2023.118529","article-title":"Unraveling the potential of microbes in decomposition of organic matter and release of carbon in the ecosystem","volume":"344","author":"Raza","year":"2023","journal-title":"J. Environ. Manag."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/jipb.12899","article-title":"Abscisic acid dynamics, signaling, and functions in plants","volume":"62","author":"Chen","year":"2020","journal-title":"J. Integr. Plant Biol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"3902","DOI":"10.1111\/pce.15388","article-title":"The Interaction Between ABA and Sugar Signalling Regulates Stomatal Production in Systemic Leaves by Controlling Sucrose Transport","volume":"48","author":"Yao","year":"2025","journal-title":"Plant Cell Environ."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"8874","DOI":"10.3390\/cimb46080524","article-title":"miRNA Sequencing Analysis in Maize Roots Treated with Neutral and Alkaline Salts","volume":"46","author":"Chen","year":"2024","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Ren, X., Fan, J., Li, X., Shan, Y., Wang, L., Ma, L., Li, Y., and Li, X. (2023). Application of RNA sequencing to understand the response of rice seedlings to salt-alkali stress. BMC Genom., 24.","DOI":"10.1186\/s12864-023-09152-4"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"tpae011","DOI":"10.1093\/treephys\/tpae011","article-title":"Na2CO3-responsive mechanism insight from quantitative proteomics and SlRUB gene function in Salix linearistipularis seedlings","volume":"44","author":"Song","year":"2024","journal-title":"Tree Physiol."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/18\/2879\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:15:23Z","timestamp":1790050523000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/18\/2879"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,20]]},"references-count":73,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["plants15182879"],"URL":"https:\/\/doi.org\/10.3390\/plants15182879","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,20]]}}}