{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:16:18Z","timestamp":1789992978249,"version":"4.0.1"},"reference-count":51,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Jiangsu Province, China","award":["BK20230756"],"award-info":[{"award-number":["BK20230756"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42307401"],"award-info":[{"award-number":["42307401"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["42307401"],"award-info":[{"award-number":["42307401"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"key technologies for \u201carbor-shrub-grass\u201d multi-layer ecological restoration in the Spartina alterniflora management area of Rudong mudflat"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Soil salinization threatens global agriculture, yet the mechanisms of salt tolerance in bioenergy crops like Miscanthus remain poorly understood. While root surface electrochemistry has been studied in crop species, its effect on salt tolerance among Miscanthus accessions remains underexplored. Therefore, this study examined whether root surface electrochemical properties are associated with differential salt tolerance and Na+ adsorption in eight Miscanthus accessions. Hydroponically grown plants were subjected to 1% NaCl for three weeks. Salt-tolerant accessions (394, 390, 422, 401) showed significantly higher relative growth parameters (81.49\u201389.21%) than salt-sensitive accessions (403, 1143, 01293, 11024; 61.82\u201366.87%; p &lt; 0.05). Root surface zeta potential measurements revealed that salt-tolerant accessions carried less negative charge (\u03b6 = \u221216.30 mV at pH 6.0) than salt-sensitive accessions (\u03b6 = \u221230.90 mV; p &lt; 0.01). Also, ATR-FTIR spectroscopy revealed a trend toward fewer ionizable functional groups, particularly carboxylates, on salt-tolerant root surfaces. Consequently, salt-sensitive accessions adsorbed significantly more Na+ (6847 mg kg\u22121) than salt-tolerant accessions (5163 mg kg\u22121; p &lt; 0.05). Moreover, significant correlations among zeta potential, Na+ adsorption, and growth indices (R2 = 0.56, p &lt; 0.5 for zeta potential vs. Na+ adsorption; R2 = 0.85, p &lt; 0.01 for zeta potential vs. RRDW; R2 = 0.65, p &lt; 0.05 for Na+ adsorption vs. RRDW) were consistent with the mechanistic link. This shows that fewer functional groups result in less negative charge and consequently reduced Na+ adsorption and enhanced salt tolerance. Therefore, salt-tolerant Miscanthus accessions possess fewer ionizable functional groups and less negative root surface charge, resulting in reduced Na+ adsorption, a mechanism not previously described in this bioenergy crop. These findings establish root surface electrochemical properties as a determinant of salt tolerance in Miscanthus and provide a rapid phenotyping tool for germplasm screening.<\/jats:p>","DOI":"10.3390\/plants15182883","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:05:53Z","timestamp":1789988753000},"page":"2883","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Root Surface Electrochemical Properties Correlate with Salt Tolerance and Sodium Adsorption in Miscanthus Accessions: A Hydroponic Study"],"prefix":"10.3390","volume":"15","author":[{"given":"Shuai","family":"Si","sequence":"first","affiliation":[{"name":"Key Laboratory of Crop Growth Regulation of Hebei Province, College of Agronomy, Hebei Agricultural University, Baoding 071000, China"},{"name":"Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Yi","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Ecology and Environment, Nanjing Forestry University, Nanjing 210037, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Yu","family":"Cheng","sequence":"additional","affiliation":[{"name":"Marine Geological Survey of Jiangsu Province, Nanjing 210007, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Hailong","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Huibin","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Crop Growth Regulation of Hebei Province, College of Agronomy, Hebei Agricultural University, Baoding 071000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Junqin","family":"Zong","sequence":"additional","affiliation":[{"name":"Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e2023RG000804","DOI":"10.1029\/2023RG000804","article-title":"Multi-Scale Soil Salinization Dynamics from Global to Pore Scale: A Review","volume":"62","author":"Shokri","year":"2024","journal-title":"Rev. Geophys."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1038\/s41597-025-06057-7","article-title":"A 40-year dataset of soil salinity dynamics (1985\u20132024) at 100 m resolution in the Western Songnen Plain, China","volume":"12","author":"Wang","year":"2025","journal-title":"Sci. Data"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e70063","DOI":"10.1002\/vzj2.70063","article-title":"Spatial heterogeneity and driving factors of soil salinity in the lowland area of North China plain","volume":"24","author":"Han","year":"2025","journal-title":"Vadose Zone J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"107222","DOI":"10.1016\/j.still.2026.107222","article-title":"Evolutionary patterns of soil salinization and saline-alkali land types in the Yellow River Delta: A spatiotemporal analysis from 2002 to 2022","volume":"262","author":"Haonan","year":"2026","journal-title":"Soil Tillage Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1007\/s11442-014-1130-2","article-title":"Soil salinization research in China: Advances and prospects","volume":"24","author":"Li","year":"2014","journal-title":"J. Geogr. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"6663","DOI":"10.1038\/s41467-021-26907-3","article-title":"Global predictions of primary soil salinization under changing climate in the 21st century","volume":"12","author":"Hassani","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"33","DOI":"10.54097\/ajst.v6i1.8279","article-title":"Progress of Water and Salt Transport in Saline Lands and Hydrus Model: A Review","volume":"6","author":"Zhang","year":"2023","journal-title":"Acad. J. Sci. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Wang, X., Chen, Z., and Sui, N. (2024). Sensitivity and responses of chloroplasts to salt stress in plants. Front. Plant Sci., 15.","DOI":"10.3389\/fpls.2024.1374086"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"110931","DOI":"10.1016\/j.plaphy.2025.110931","article-title":"Dopamine regulates nitric oxide biosynthesis, optimizes carbon utilization, and maintains potassium retention to support wheat productivity under salt stress","volume":"230","author":"Kumari","year":"2026","journal-title":"Plant Physiol. Biochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s11104-009-0076-0","article-title":"Mechanisms of sodium uptake by roots of higher plants","volume":"326","author":"Zhang","year":"2010","journal-title":"Plant Soil"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1006\/anbo.1999.0912","article-title":"K+ nutrition and Na+ toxicity: The basis of cellular K+\/Na+ ratios","volume":"84","author":"Maathuis","year":"1999","journal-title":"Ann. Bot."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Wang, L., Liu, Y., Li, D., Feng, S., Yang, J., Zhang, J., Zhang, J., Wang, D., and Gan, Y. (2019). Improving salt tolerance in potato through overexpression of AtHKT1 gene. BMC Plant Biol., 19.","DOI":"10.1186\/s12870-019-1963-z"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"34548","DOI":"10.1038\/srep34548","article-title":"Na+ compartmentalization related to salinity stress tolerance in upland cotton (Gossypium hirsutum) seedlings","volume":"6","author":"Peng","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.cj.2021.03.005","article-title":"Molecular mechanisms of salinity tolerance in rice","volume":"9","author":"Chen","year":"2021","journal-title":"Crop J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"114305","DOI":"10.1016\/j.indcrop.2021.114305","article-title":"Transcriptomic and physiological analyses of Miscanthus lutarioriparius in response to plumbum stress","volume":"176","author":"Wang","year":"2022","journal-title":"Ind. Crops Prod."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Yang, S., Xue, S., Kang, W., Qian, Z., and Yi, Z. (2019). Genetic diversity and population structure of Miscanthus lutarioriparius, an endemic plant of China. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0211471"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5","DOI":"10.15376\/biores.16.1.5-8","article-title":"Miscanthus: Beyond its Use as an Energy Crop","volume":"16","author":"Sa","year":"2021","journal-title":"Bioresources"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2000","DOI":"10.1111\/j.1365-2486.2008.01662.x","article-title":"Meeting US biofuel goals with less land: The potential of Miscanthus","volume":"14","author":"Heaton","year":"2008","journal-title":"Glob. Change Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"107898","DOI":"10.1016\/j.biombioe.2025.107898","article-title":"Deciphering the physiological response mechanisms of Miscanthus sinensis to salt-induced stress","volume":"199","author":"Lu","year":"2025","journal-title":"Biomass Bioenergy"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Lu, H., Li, L., Chen, J., Nkoh, J.N., Hao, D., Li, J., Wang, J., Li, D., Liu, J., and Guo, H. (2024). Effect of Salt-Induced Stress on the Calorific Value of Two Miscanthus sacchariflorus (Amur Silvergrass) Varieties. Agronomy, 14.","DOI":"10.3390\/agronomy14061259"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"111224","DOI":"10.1016\/j.ecoenv.2020.111224","article-title":"Effects of surface charge and chemical forms of manganese(II) on rice roots on manganese absorption by different rice varieties","volume":"207","author":"Lu","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7022","DOI":"10.1007\/s11356-015-6008-z","article-title":"Changes in the chemical properties and swelling coefficient of alfalfa root cell walls in the presence of toluene as a toxic agent","volume":"23","author":"Sharifi","year":"2016","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s11104-014-2259-6","article-title":"Zeta potential at the root surfaces of rice characterized by streaming potential measurements","volume":"386","author":"Li","year":"2015","journal-title":"Plant Soil"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Liu, Z.-D., Zhou, Q., Hong, Z.-N., and Xu, R.-K. (2017). Effects of Surface Charge and Functional Groups on the Adsorption and Binding Forms of Cu and Cd on Roots of indica and japonica Rice Cultivars. Front Plant Sci., 8.","DOI":"10.3389\/fpls.2017.01489"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s11104-018-3676-8","article-title":"Zeta potential of roots determined by the streaming potential method in relation to their Mn(II) sorption in 17 crops","volume":"428","author":"Lu","year":"2018","journal-title":"Plant Soil"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s11104-016-2909-y","article-title":"The effects of root surface charge and nitrogen forms on the adsorption of aluminum ions by the roots of rice with different aluminum tolerances","volume":"408","author":"Liu","year":"2016","journal-title":"Plant Soil"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"123758","DOI":"10.1016\/j.indcrop.2026.123758","article-title":"Hydroponic electrochemical phenotyping of root surfaces for rapid screening of salt-tolerant rice (Oryza sativa L.)","volume":"249","author":"Lu","year":"2026","journal-title":"Ind. Crops Prod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0144-8617(00)00151-X","article-title":"FT-IR study of plant cell wall model compounds: Pectic polysaccharides and hemicelluloses","volume":"43","year":"2000","journal-title":"Carbohydr. Polym."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"103915","DOI":"10.1016\/j.envexpbot.2019.103915","article-title":"Effect of plant sample preparation and measuring methods on ATR-FTIR spectra results","volume":"169","author":"Durak","year":"2020","journal-title":"Environ. Exp. Bot."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Xu, Y., Bu, W., Xu, Y., Fei, H., Zhu, Y., Ahmad, I., Nimir, N.E.A., Zhou, G., and Zhu, G. (2024). Effects of Salt Stress on Physiological and Agronomic Traits of Rice Genotypes with Contrasting Salt Tolerance. Plants, 13.","DOI":"10.3390\/plants13081157"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.fcr.2011.11.004","article-title":"Isolation, characterization, and field evaluation of rice (Oryza sativa L., Var. IR64) mutants with altered responses to salt stress","volume":"127","author":"Nakhoda","year":"2012","journal-title":"Field Crops Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"31036","DOI":"10.1038\/s41598-024-82173-5","article-title":"Comparative morphological, physiological, and biochemical traits in sensitive and tolerant maize genotypes in response to salinity and Pb stress","volume":"14","author":"Zamani","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Sparks, D.L., Singh, B., and Siebecker, M.G. (2024). An Introduction to Environmental Soil Chemistry. Environmental Soil Chemistry, Elsevier.","DOI":"10.1016\/B978-0-443-14034-1.00001-0"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s11104-011-1001-x","article-title":"Interactions between Ca, Mg, Na and K: Alleviation of toxicity in saline solutions","volume":"352","author":"Kopittke","year":"2012","journal-title":"Plant Soil"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1111\/pce.12490","article-title":"Altered cell wall properties are responsible for ammonium-reduced aluminium accumulation in rice roots","volume":"38","author":"Wang","year":"2015","journal-title":"Plant Cell Environ."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"36373","DOI":"10.1038\/srep36373","article-title":"Relative abundance of chemical forms of Cu(II) and Cd(II) on soybean roots as influenced by pH, cations and organic acids","volume":"6","author":"Zhou","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"111371","DOI":"10.1016\/j.plantsci.2022.111371","article-title":"Auxin facilitates cell wall phosphorus reutilization in a nitric oxide-ethylene dependent manner in phosphorus deficient rice (Oryza sativa L.)","volume":"322","author":"Huang","year":"2022","journal-title":"Plant Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.1105\/tpc.114.127993","article-title":"TAA1-regulated local auxin biosynthesis in the root-apex transition zone mediates the aluminum-induced inhibition of root growth in Arabidopsis","volume":"26","author":"Yang","year":"2014","journal-title":"Plant Cell"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/tpj.15424","article-title":"Local regulation of auxin transport in root-apex transition zone mediates aluminium-induced Arabidopsis root-growth inhibition","volume":"108","author":"Li","year":"2021","journal-title":"Plant J."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"109366","DOI":"10.1016\/j.plaphy.2024.109366","article-title":"Glutathione mitigates aluminum toxicity in root-apex transition zone of rice through reducing aluminum absorption and maintaining redox balance","volume":"219","author":"Jiang","year":"2025","journal-title":"Plant Physiol. Biochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1093\/plcell\/koad317","article-title":"Root branching under high salinity requires auxin-independent modulation of LATERAL ORGAN BOUNDARY DOMAIN 16 function","volume":"36","author":"Zhang","year":"2024","journal-title":"Plant Cell"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Liu, J., Otie, V., Matsuura, A., Junichi, K., Irshad, M., Zheng, Y., Fujimaki, H., and An, P. (2022). Pectin Characteristics Affect Root Growth in Spinach under Salinity. Plants, 11.","DOI":"10.3390\/plants11223130"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1901455","DOI":"10.1002\/advs.201901455","article-title":"Root Growth Adaptation is Mediated by PYLs ABA Receptor-PP2A Protein Phosphatase Complex","volume":"7","author":"Li","year":"2020","journal-title":"Adv. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"827","DOI":"10.32604\/phyton.2021.013805","article-title":"Differential responses of NHX1 and SOS1 gene expressions to salinity in two Miscanthus sinensis anderss. Accessions with different salt tolerance","volume":"90","author":"Sun","year":"2021","journal-title":"Phyton"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"123934","DOI":"10.1016\/j.indcrop.2026.123934","article-title":"Multi-omics analysis reveals key genomic insights into salt tolerance mechanisms in Miscanthus sacchariflorus and M. lutarioriparius","volume":"250","author":"Tang","year":"2026","journal-title":"Ind. Crops Prod."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"37589","DOI":"10.1038\/s41598-025-17012-2","article-title":"Biochemical changes and phytoextraction potential of quinoa and wheat for their resilience to salt stress","volume":"15","author":"Hosseini","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"16","DOI":"10.36959\/451\/696","article-title":"Salt Stress Induces Shoot Formation and Physiological and Biochemical Changes in Miscanthus sacchariflorus","volume":"1","author":"Hong","year":"2019","journal-title":"J. Plant Biotechnol. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"115590","DOI":"10.1016\/j.envpol.2020.115590","article-title":"Plants alter surface charge and functional groups of their roots to adapt to acidic soil conditions","volume":"267","author":"Lu","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/0376-7388(96)00127-5","article-title":"Effect of solution chemistry on the surface charge of polymeric reverse osmosis and nanofiltration membranes","volume":"119","author":"Childress","year":"1996","journal-title":"J. Memb. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1002\/bbb.1353","article-title":"Miscanthus: A fast-growing crop for biofuels and chemicals production","volume":"6","author":"Brosse","year":"2012","journal-title":"Biofuels Bioprod. Biorefin."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1111\/gcbb.12761","article-title":"Miscanthus: A fast-growing crop for environmental remediation and biofuel production","volume":"13","author":"Wang","year":"2021","journal-title":"GCB Bioenergy"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/18\/2883\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:56:53Z","timestamp":1789991813000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/18\/2883"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,21]]},"references-count":51,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["plants15182883"],"URL":"https:\/\/doi.org\/10.3390\/plants15182883","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,21]]}}}