{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:09:22Z","timestamp":1790759362692,"version":"4.1.0"},"reference-count":66,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Agriculture and Rural Affairs of Liaoning Province","award":["2026-NZZY-05"],"award-info":[{"award-number":["2026-NZZY-05"]}]},{"name":"Liaoning Provincial Academy of Agricultural Sciences","award":["2025QN2603"],"award-info":[{"award-number":["2025QN2603"]}]},{"name":"Liaoning Provincial Academy of Agricultural Sciences","award":["2025MS1703"],"award-info":[{"award-number":["2025MS1703"]}]},{"award":["2025QN2603"],"award-info":[{"award-number":["2025QN2603"]}],"id":[{"id":"https:\/\/ror.org\/03vnb1535","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2025MS1703"],"award-info":[{"award-number":["2025MS1703"]}],"id":[{"id":"https:\/\/ror.org\/03vnb1535","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Long-term continuous monocropping of foxtail millet (Setaria italica) in China has increased crop vulnerability to emerging diseases and threatens yield stability and grain quality. This practice may also influence inoculum carryover and host\u2013pathogen interactions, making foxtail millet health a central issue for sustainable production. This study reports the first occurrence in China of a previously undescribed bacterial leaf disease of foxtail millet. Early symptoms are characterized by water-soaked lesions at leaf tips that spread basipetally along the veins and gradually desiccate as the tissue dehydrates. Koch\u2019s postulates were fulfilled to isolate and identify the causal agent. Multilocus sequence analysis of three concatenated housekeeping genes (gyrB, infB, and rpoB) classified the pathogen as Pantoea ananatis. The isolate was further characterized for metabolic profile; its colonization pattern and spatial distribution within foxtail millet tissues were elucidated, revealing features of host\u2013pathogen interaction. A high-quality de novo whole-genome assembly was generated, and candidate virulence determinants were identified by integrated genome annotation and cell wall hydrolase activity assays. This genomic resource is intended as a supporting tool for plant protection research, including disease surveillance, resistance breeding, and management. Collectively, this study represents the first report of P. ananatis causing bacterial leaf disease on foxtail millet in China. It clarifies the colonization and distribution of the pathogen in host tissues, reveales reveals its interaction characteristics with foxtail millet, and provided genomic resources to support research on foxtail millet health protection and plant protection under continuous cropping conditions.<\/jats:p>","DOI":"10.3390\/plants15192986","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:17:27Z","timestamp":1790756247000},"page":"2986","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A New Leaf Bacterial Disease of Foxtail Millet Caused by Pantoea ananatis in China"],"prefix":"10.3390","volume":"15","author":[{"given":"Ning","family":"Han","sequence":"first","affiliation":[{"name":"College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China"},{"name":"Liaoning Institute Sandy Land Control and Utilization, Fuxin 123000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Guo-Qing","family":"Yu","sequence":"additional","affiliation":[{"name":"Liaoning Institute Sandy Land Control and Utilization, Fuxin 123000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi-Wang","family":"Ke","sequence":"additional","affiliation":[{"name":"College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Wen","family":"Zhuang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9765-7214","authenticated-orcid":false,"given":"Hong-Xi","family":"Sun","sequence":"additional","affiliation":[{"name":"Liaoning Institute Sandy Land Control and Utilization, Fuxin 123000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Hao","family":"Lu","sequence":"additional","affiliation":[{"name":"Liaoning Institute Sandy Land Control and Utilization, Fuxin 123000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Ren","sequence":"additional","affiliation":[{"name":"Liaoning Institute Sandy Land Control and Utilization, Fuxin 123000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Yu-Hu","family":"Zuo","sequence":"additional","affiliation":[{"name":"College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3726","DOI":"10.1073\/pnas.1115430109","article-title":"Early Millet Use in Northern China","volume":"109","author":"Yang","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_2","first-page":"459","article-title":"Current Status and Future Prospective of Foxtail Millet Production and Seed Industry in China","volume":"54","author":"Li","year":"2021","journal-title":"Sci. Agric. Sin."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.cj.2016.06.004","article-title":"Production and Genetic Improvement of Minor Cereals in China","volume":"5","author":"Diao","year":"2017","journal-title":"Crop J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"165","DOI":"10.18811\/ijpen.v6i03.01","article-title":"Foxtail Millet: A Climate-Resilient Crop Species with Potential to Ensure Food and Agriculture Security Amidst Global Climate Change","volume":"6","author":"Singh","year":"2020","journal-title":"Int. J. Plant Environ."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1007\/s00374-025-01904-6","article-title":"Pathogenic Fungi Accumulation Rather than Direct Autotoxicity Is Responsible for the Root-Rot Disease of Foxtail Millet under Continuous Monoculture Regimes","volume":"61","author":"Yang","year":"2025","journal-title":"Biol. Fertil. Soils"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/s10725-022-00936-4","article-title":"Rhizosphere Soil Microbial Communities under Foxtail Millet Continuous and Rotational Cropping Systems and Their Feedback Effects on Foxtail Millet Downy Mildew Suppression","volume":"99","author":"Xu","year":"2023","journal-title":"Plant Growth Regul."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/j.1364-3703.2009.00542.x","article-title":"Pantoea ananatis: An Unconventional Plant Pathogen","volume":"10","author":"Coutinho","year":"2009","journal-title":"Mol. Plant Pathol."},{"key":"ref_8","first-page":"271","article-title":"Bacterial Fruitlet Brown-Rot of Pineapple in the Philippines","volume":"36","author":"Serrano","year":"1928","journal-title":"Philipp. J. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.tree.2004.07.021","article-title":"Emerging Infectious Diseases of Plants: Pathogen Pollution, Climate Change and Agrotechnology Drivers","volume":"19","author":"Anderson","year":"2004","journal-title":"Trends Ecol. Evol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1094\/PDIS-06-11-0533","article-title":"A New Leaf Blight of Rice Caused by Pantoea ananatis in India","volume":"95","author":"Mondal","year":"2011","journal-title":"Plant Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1080\/07060661.2022.2096697","article-title":"First Report of Rice Grain Discolouration and Leaf Blight Caused by Pantoea ananatis in the Kuttanad Agro-Ecosystem, Kerala, India","volume":"45","author":"Reshma","year":"2023","journal-title":"Can. J. Plant Pathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2214","DOI":"10.1094\/PDIS-08-22-2014-PDN","article-title":"First Report of Rice Bacterial Leaf Blight Disease Caused by Pantoea ananatis in the United States","volume":"107","author":"Luna","year":"2023","journal-title":"Plant Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1094\/PDIS-05-19-1038-PDN","article-title":"First Report of a Leaf Blight in Rice (Oryza sativa) Caused by Pantoea ananatis and Pantoea stewartii in Thailand","volume":"104","author":"Arayaskul","year":"2020","journal-title":"Plant Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1094\/PDIS-05-21-0988-PDN","article-title":"First Report of New Bacterial Leaf Blight of Rice Caused by Pantoea ananatis in Southeast China","volume":"106","author":"Yu","year":"2022","journal-title":"Plant Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3286","DOI":"10.1094\/PDIS-02-21-0298-PDN","article-title":"First Report of Pantoea ananatis Causing Leaf Spot Disease of Maize in Ecuador","volume":"105","author":"Toaza","year":"2021","journal-title":"Plant Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1021\/np100571v","article-title":"Lupane Triterpenes with a \u03b4-Lactone at Ring E, from Lippia mexicana","volume":"73","author":"Maldonado","year":"2010","journal-title":"J. Nat. Prod."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1094\/PDIS-01-22-0152-PDN","article-title":"First Report of Maize White Spot Disease Caused by Pantoea ananatis in China","volume":"107","author":"Cui","year":"2023","journal-title":"Plant Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1094\/PDIS-94-4-0487A","article-title":"First Report of Leaf Spot Disease of Maize Caused by Pantoea ananatis in Argentina","volume":"94","author":"Alippi","year":"2010","journal-title":"Plant Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"105431","DOI":"10.1016\/j.cropro.2020.105431","article-title":"Transmission of Pantoea ananatis, the Causal Agent of Leaf Spot Disease of Maize (Zea mays), by Western Corn Rootworm (Diabrotica virgifera virgifera LeConte)","volume":"141","author":"Krawczyk","year":"2021","journal-title":"Crop Prot."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2913","DOI":"10.1094\/PDIS-04-24-0903-PDN","article-title":"First Report of Pantoea ananatis Causing Leaf Streak Disease on Wheat (Triticum aestivum) in the United States of America","volume":"108","author":"Alhusays","year":"2024","journal-title":"Plant Dis."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Krawczyk, K., Wielkopolan, B., and Obr\u0119palska-St\u0119plowska, A. (2020). Pantoea Ananatis, A New Bacterial Pathogen Affecting Wheat Plants (Triticum L.) in Poland. Pathogens, 9.","DOI":"10.3390\/pathogens9121079"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/S0261-2194(02)00078-9","article-title":"Recovery of Pantoea ananatis, Causal Agent of Center Rot of Onion, from Weeds and Crops in Georgia, USA","volume":"21","author":"Gitaitis","year":"2002","journal-title":"Crop Prot."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.1128\/JB.00060-10","article-title":"Genome Sequence of Pantoea ananatis LMG20103, the Causative Agent of Eucalyptus Blight and Dieback","volume":"192","author":"Chan","year":"2010","journal-title":"J. Bacteriol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e00489-14","DOI":"10.1128\/genomeA.00489-14","article-title":"Draft Genome Sequence of Pantoea Ananatis Strain LMG 2665T, a Bacterial Pathogen of Pineapple Fruitlets","volume":"2","author":"Adam","year":"2014","journal-title":"Genome Announc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1111\/mpp.12517","article-title":"Pantoea ananatis: Genomic Insights into a Versatile Pathogen","volume":"18","author":"Coutinho","year":"2017","journal-title":"Mol. Plant Pathol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"De Maayer, P., Chan, W.Y., Rubagotti, E., Venter, S.N., Toth, I.K., Birch, P.R.J., and Coutinho, T.A. (2014). Analysis of the Pantoea ananatis Pan-Genome Reveals Factors Underlying Its Ability to Colonize and Interact with Plant, Insect and Vertebrate Hosts. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-404"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s13068-016-0439-8","article-title":"Genomic and Secretomic Insight into Lignocellulolytic System of an Endophytic Bacterium Pantoea ananatis Sd-1","volume":"9","author":"Ma","year":"2016","journal-title":"Biotechnol. Biofuels"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"15017863","DOI":"10.4238\/gmr.15017863","article-title":"Characterization of the inaA Gene and Expression of Ice Nucleation Phenotype in Pantoea ananatis Isolates from Maize White Spot Disease","volume":"15","author":"Miller","year":"2016","journal-title":"Genet. Mol. Res."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"De Maayer, P., Venter, S.N., Kamber, T., Duffy, B., Coutinho, T.A., and Smits, T.H.M. (2011). Comparative Genomics of the Type VI Secretion Systems of Pantoea and Erwinia Species Reveals the Presence of Putative Effector Islands That May Be Translocated by the VgrG and Hcp Proteins. BMC Genom., 12.","DOI":"10.1186\/1471-2164-12-576"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Shyntum, D.Y., Venter, S.N., Moleleki, L.N., Toth, I., and Coutinho, T.A. (2014). Comparative Genomics of Type VI Secretion Systems in Strains of Pantoea ananatis from Different Environments. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-163"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1094\/MPMI-07-14-0219-R","article-title":"Pantoea ananatis Utilizes a Type VI Secretion System for Pathogenesis and Bacterial Competition","volume":"28","author":"Shyntum","year":"2015","journal-title":"Mol. Plant Microbe Interact."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/nrg.2016.49","article-title":"Coming of Age: Ten Years of next-Generation Sequencing Technologies","volume":"17","author":"Goodwin","year":"2016","journal-title":"Nat. Rev. Genet."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1128\/MMBR.68.4.686-691.2004","article-title":"Status of the Microbial Census","volume":"68","author":"Schloss","year":"2004","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/nature02340","article-title":"Community Structure and Metabolism through Reconstruction of Microbial Genomes from the Environment","volume":"428","author":"Tyson","year":"2004","journal-title":"Nature"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Li, Y., Han, Y., Qu, M., Chen, J., Chen, X., Geng, X., Wang, Z., and Chen, S. (2020). Apoplastic Cell Death-Inducing Proteins of Filamentous Plant Pathogens: Roles in Plant-Pathogen Interactions. Front. Genet., 11.","DOI":"10.3389\/fgene.2020.00661"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1111\/j.1364-3703.2009.00591.x","article-title":"Fungal Effector Proteins: Past, Present and Future","volume":"10","author":"Mehrabi","year":"2009","journal-title":"Mol. Plant Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1146\/annurev-arplant-043014-114623","article-title":"Fungal Effectors and Plant Susceptibility","volume":"66","author":"Lanver","year":"2015","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"D233","DOI":"10.1093\/nar\/gkn663","article-title":"The Carbohydrate-Active EnZymes Database (CAZy): An Expert Resource for Glycogenomics","volume":"37","author":"Cantarel","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1094\/Phyto-77-511","article-title":"Isolation and Characterization of Strains of Erwinia ananas from Honeydew Melons","volume":"77","author":"Wells","year":"1987","journal-title":"Phytopathology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1046\/j.1439-0434.2001.00614.x","article-title":"Detection of a Bacterium Associated with a Leaf Spot Disease of Maize in Brazil","volume":"149","author":"Ferreira","year":"2001","journal-title":"J. Phytopathol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1094\/PDIS.2000.84.9.973","article-title":"A New Leaf Blotch Disease of Sudangrass Caused by Pantoea ananas and Pantoea stewartii","volume":"84","author":"Azad","year":"2000","journal-title":"Plant Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1007\/s10327-008-0107-3","article-title":"Internal Fruit Rot of Netted Melon Caused by Pantoea ananatis (=Erwinia ananas) in Japan","volume":"74","author":"Kido","year":"2008","journal-title":"J. Gen. Plant Pathol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1631\/jzus.B2100682","article-title":"Shoot Rot of Zizania Latifolia and the First Record of Its Pathogen Pantoea ananatis in China","volume":"23","author":"Xiao","year":"2022","journal-title":"J. Zhejiang Univ.-SCI. B"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1071\/AP04053","article-title":"An Unusual Stem Necrosis of Rice Caused by Pantoea ananas and the First Record of This Pathogen on Rice in Australia","volume":"33","author":"Cother","year":"2004","journal-title":"Australas. Plant Pathol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Duan, G., Liu, X., Zhang, S., Chai, M., Peng, Z., Lin, Z., Li, D., and Sun, W. (2025). An Emerging Bacterial Leaf Disease in Rice Caused by Pantoea ananatis and Pantoea eucalypti in Northeast China. Microorganisms, 13.","DOI":"10.3390\/microorganisms13061376"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3736","DOI":"10.1094\/PDIS-03-21-0669-PDN","article-title":"First Report of Pearl Millet Bacterial Leaf Blight Caused by Pantoea stewartii Subspecies indologenes in India","volume":"105","author":"Ashajyothi","year":"2021","journal-title":"Plant Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/j.tim.2005.09.005","article-title":"Type II Secretion: A Protein Secretion System for All Seasons","volume":"13","author":"Cianciotto","year":"2005","journal-title":"Trends Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.bbamcr.2004.05.003","article-title":"The Underlying Mechanisms of Type II Protein Secretion","volume":"1694","author":"Filloux","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1146\/annurev.arplant.57.032905.105346","article-title":"A Renaissance of Elicitors: Perception of Microbe-Associated Molecular Patterns and Danger Signals by Pattern-Recognition Receptors","volume":"60","author":"Boller","year":"2009","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Bellincampi, D., Cervone, F., and Lionetti, V. (2014). Plant Cell Wall Dynamics and Wall-Related Susceptibility in Plant\u2013pathogen Interactions. Front. Plant Sci., 5.","DOI":"10.3389\/fpls.2014.00228"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.tplants.2004.02.005","article-title":"The Role of Plant Cell Wall Polysaccharide Composition in Disease Resistance","volume":"9","author":"Vorwerk","year":"2004","journal-title":"Trends Plant Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.1111\/mpp.12436","article-title":"Bacterial Disease Management: Challenges, Experience, Innovation and Future Prospects","volume":"17","author":"Sundin","year":"2016","journal-title":"Mol. Plant Pathol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1094\/PDIS-05-15-0574-FE","article-title":"Toward a Reduced Reliance on Conventional Pesticides in European Agriculture","volume":"100","author":"Lamichhane","year":"2016","journal-title":"Plant Dis."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1146\/annurev.phyto.45.062806.094321","article-title":"The Epidemiology and Management of Seedborne Bacterial Diseases","volume":"45","author":"Gitaitis","year":"2007","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_55","unstructured":"Schaad, N.W., Jones, J.B., and Chun, W. (2001). Laboratory Guide for Identification of Plant Pathogenic Bacteria, American Phytopathological Society Press. [3rd ed.]. Available online: http:\/\/www.scisoc.org."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.shpsc.2016.06.001","article-title":"Koch\u2019s Postulates: An Interventionist Perspective","volume":"59","author":"Ross","year":"2016","journal-title":"Stud. Hist. Philos. Sci. C Stud. Hist. Philos. Biol. Biomed. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.syapm.2008.09.004","article-title":"Phylogeny and Identification of Pantoea Species Associated with Plants, Humans and the Natural Environment Based on Multilocus Sequence Analysis (MLSA)","volume":"31","author":"Brady","year":"2008","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/br.16.1.1-29.1952","article-title":"The Gram Stain","volume":"16","author":"Bartholomew","year":"1952","journal-title":"Bacteriol. Rev."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e87","DOI":"10.1002\/imt2.87","article-title":"Using PhyloSuite for Molecular Phylogeny and Tree-Based Analyses","volume":"2","author":"Xiang","year":"2023","journal-title":"iMeta"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/molbev\/msu300","article-title":"IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies","volume":"32","author":"Nguyen","year":"2015","journal-title":"Mol. Biol. Evol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Wick, R.R., Judd, L.M., Gorrie, C.L., and Holt, K.E. (2017). Unicycler: Resolving Bacterial Genome Assemblies from Short and Long Sequencing Reads. PLoS Comput. Biol., 13.","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"9077","DOI":"10.1093\/nar\/gkab688","article-title":"tRNAscan-SE 2.0: Improved Detection and Functional Classification of Transfer RNA Genes","volume":"49","author":"Chan","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"W81","DOI":"10.1093\/nar\/gkz310","article-title":"antiSMASH 5.0: Updates to the Secondary Metabolite Genome Mining Pipeline","volume":"47","author":"Blin","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2811","DOI":"10.1093\/bioinformatics\/btu393","article-title":"Circlize Implements and Enhances Circular Visualization in R","volume":"30","author":"Gu","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: Rapid Prokaryotic Genome Annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1094\/PDIS-10-20-2292-RE","article-title":"Enterobacter asburiae and Pantoea ananatis Causing Rice Bacterial Blight in China","volume":"105","author":"Xue","year":"2021","journal-title":"Plant Dis."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/19\/2986\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:53:46Z","timestamp":1790758426000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/15\/19\/2986"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":66,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["plants15192986"],"URL":"https:\/\/doi.org\/10.3390\/plants15192986","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]}}}