{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T22:30:38Z","timestamp":1766788238249,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,7,6]],"date-time":"2019-07-06T00:00:00Z","timestamp":1562371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009882","name":"Regione Lombardia","doi-asserted-by":"publisher","award":["15083\/RCC"],"award-info":[{"award-number":["15083\/RCC"]}],"id":[{"id":"10.13039\/501100009882","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Phytic acid, or myo-inositol 1,2,3,4,5,6-hexakisphosphate, is the main storage form of phosphorus in plants. It is localized in seeds, deposited as mixed salts of mineral cations in protein storage vacuoles; during germination, it is hydrolyzed by phytases to make available P together with all the other cations needed for seed germination. When seeds are used as food or feed, phytic acid and the bound cations are poorly bioavailable for human and monogastric livestock due to their lack of phytase activity. Therefore, reducing the amount of phytic acid is one strategy in breeding programs aimed to improve the nutritional properties of major crops. In this work, we present data on the isolation of a new maize (Zea mays L.) low phytic acid 1 (lpa1) mutant allele obtained by transposon tagging mutagenesis with the Ac element. We describe the generation of the mutagenized population and the screening to isolate new lpa1 mutants. In particular, we developed a fast, cheap and non-disrupting screening method based on the different density of lpa1 seed compared to the wild type. This assay allowed the isolation of the lpa1-5525 mutant characterized by a new mutation in the lpa1 locus associated with a lower amount of phytic phosphorus in the seeds in comparison with the wild type.<\/jats:p>","DOI":"10.3390\/plants8070209","type":"journal-article","created":{"date-parts":[[2019,7,8]],"date-time":"2019-07-08T03:01:31Z","timestamp":1562554891000},"page":"209","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["lpa1-5525: A New lpa1 Mutant Isolated in a Mutagenized Population by a Novel Non-Disrupting Screening Method"],"prefix":"10.3390","volume":"8","author":[{"given":"Giulia","family":"Borlini","sequence":"first","affiliation":[{"name":"Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy-Universit\u00e0 degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3595-8564","authenticated-orcid":false,"given":"Cesare","family":"Rovera","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy-Universit\u00e0 degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Landoni","sequence":"additional","affiliation":[{"name":"Department of Biosciences-Universit\u00e0 degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Cassani","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy-Universit\u00e0 degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Pilu","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy-Universit\u00e0 degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1021\/jf60181a021","article-title":"Distribution of phytate and nutritionally important elements among the morphological components of cereal grains","volume":"20","author":"Koirtyohann","year":"1972","journal-title":"J. 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