{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:02:48Z","timestamp":1787029368110,"version":"build-2736575974"},"reference-count":43,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,11,15]],"date-time":"2019-11-15T00:00:00Z","timestamp":1573776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Spontaneous edible plants have an old history of use in popular traditions all around the world, and the rediscovery of these species could also be useful for the search of new drugs. Chenopodium album L. (Amaranthaceae) and Sisymbrium officinale (L.) Scop. (Brassicaceae) are two annual plants traditionally used both as food and herbal remedies against inflammatory disorders. In this work, the potential anti-inflammatory and anti-arthritic activities of these plant species have been investigated, together with their antioxidant potential. The phytochemical composition was assessed as well by means of gas chromatography coupled to mass spectrometry (GC-MS) and high performance thin layer chromatography (HPTLC). The antioxidant properties were assessed using the DPPH and \u03b2-carotene bleaching test. The ability of extracts to protect against lipid peroxidation was also examined in rat-liver microsomal membranes. All the samples showed a preservation of antioxidant activity up to 60 min. A significant inhibitory activity on the production of the pro-inflammatory mediator nitric oxide was induced in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells by the dichloromethane fraction of C. album extract, with an IC50 value equal to 81.7 \u00b1 0.9 \u03bcg\/mL. The same sample showed also a concentration-dependent anti-denaturation effect on heat-treated bovine serum albumin (IC50 = 975.6 \u00b1 5.5 \u03bcg\/mL), even if the best in vitro anti-arthritic activity was observed for the dichloromethane fraction of S. officinale extract, with an IC50 value of 680.9 \u00b1 13.2 \u03bcg\/mL.<\/jats:p>","DOI":"10.3390\/plants8110505","type":"journal-article","created":{"date-parts":[[2019,11,15]],"date-time":"2019-11-15T11:25:56Z","timestamp":1573817156000},"page":"505","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Chenopodium album L. and Sisymbrium officinale (L.) Scop.: Phytochemical Content and In Vitro Antioxidant and Anti-Inflammatory Potential"],"prefix":"10.3390","volume":"8","author":[{"given":"Valentina","family":"Amodeo","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3741-4261","authenticated-orcid":false,"given":"Mariangela","family":"Marrelli","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Pontieri","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6980-6799","authenticated-orcid":false,"given":"Roberta","family":"Cassano","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Trombino","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6699-005X","authenticated-orcid":false,"given":"Filomena","family":"Conforti","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giancarlo","family":"Statti","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1007\/s11284-009-0666-6","article-title":"Architecture and growth of an annual plant Chenopodium album in different light climates","volume":"25","author":"Nishimura","year":"2010","journal-title":"Ecol. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1080\/10412905.2011.9700480","article-title":"Anti-inflammatory activity of some essential oils","volume":"23","author":"Zavala","year":"2011","journal-title":"J. Essent. Oil Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.jep.2008.08.024","article-title":"Plants used as remedies antirheumatic and antineuralgic in the traditional medicine of Lebanon","volume":"120","author":"Nelly","year":"2008","journal-title":"J. Ethnopharmacol."},{"key":"ref_4","first-page":"71","article-title":"Nutritional value and nitrate content in edible wild species used in southern Italy. III International Symposium Diversification of Vegetable Crops","volume":"467","author":"Bianco","year":"1996","journal-title":"Acta Hortic."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"00243","DOI":"10.15406\/joentr.2017.08.00243","article-title":"Sisymbrium \u201cSingers\u2019 Plant\u201d Efficacy in Reducing Perceived Vocal Tract Disability","volume":"8","author":"Calcinoni","year":"2017","journal-title":"J. Otolaryngol. ENT Res."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Guarise, M., Borgonovo, G., Bassoli, A., and Ferrante, A. (2019). Evaluation of two wild populations of Hedge Mustard (Sisymbrium officinale (L.) Scop.) as a potential leafy vegetable. Horticulturae, 5.","DOI":"10.3390\/horticulturae5010013"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.jep.2009.12.001","article-title":"Pharmacological and phytochemical study on a Sisymbrium officinale Scop. extract","volume":"127","author":"Vitalone","year":"2010","journal-title":"J. Ethnopharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1663\/0013-0001(2004)058[0684:TUOWEP]2.0.CO;2","article-title":"The use of wild edible plants in western and central Anatolia (Turkey)","volume":"58","author":"Dogan","year":"2004","journal-title":"Econ. Bot."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/BF02864545","article-title":"Prospects for sustainable use and development of wild food plants in Ethiopia","volume":"55","author":"Asfaw","year":"2001","journal-title":"Econ. Bot."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1746-4269-4-13","article-title":"Traditional knowledge of wild edible plants used in Palestine (Northern West Bank): A comparative study","volume":"4","author":"Jamous","year":"2008","journal-title":"J. Ethnobiol. Ethnomed."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.tplants.2017.01.001","article-title":"Ethnobotany, phylogeny, and \u2018omics\u2019 for human health and food security","volume":"22","author":"Garnatje","year":"2017","journal-title":"Trends Plant Sci."},{"key":"ref_12","first-page":"22","article-title":"A vindication of ethnobotany: Between natural and social science","volume":"6","author":"Garnatje","year":"2016","journal-title":"M\u00e8tode Sci. Stud. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.jep.2014.05.026","article-title":"Involvement of NF\u03baB in the antirheumatic potential of Chenopodium album L. aerial parts extracts","volume":"155","author":"Arora","year":"2014","journal-title":"J. Ethnopharmacol."},{"key":"ref_14","first-page":"30","article-title":"Different approaches to study the traditional remedy of \u2018hierba del canto\u2019, Sisymbrium officinale (L.) Scop","volume":"7","author":"Politi","year":"2008","journal-title":"Bol. Latinoam. Caribe Plantas Med. Arom\u00e1t."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.fct.2015.09.011","article-title":"Inhibitory effects of wild dietary plants on lipid peroxidation and on the proliferation of human cancer cells","volume":"86","author":"Marrelli","year":"2015","journal-title":"Food Chem. Toxicol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1080\/11263504.2017.1330778","article-title":"Seasonal and environmental variability of non-cultivated edible Cichorioideae (Asteraceae)","volume":"152","author":"Marrelli","year":"2018","journal-title":"Plant Biosyst."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1007\/s11130-017-0618-1","article-title":"Investigation of the potential health benefits as lipase inhibitor and antioxidant of Leopoldia comosa (L.) Parl.: Variability of chemical composition of wild and cultivated bulbs","volume":"72","author":"Marrelli","year":"2017","journal-title":"Plant Foods Hum. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1002\/ptr.3603","article-title":"Wild Mediterranean dietary plants as inhibitors of pancreatic lipase","volume":"26","author":"Conforti","year":"2012","journal-title":"Phytother. Res."},{"key":"ref_19","unstructured":"(2012). The modern analytical determination of botanicals and similar novel natural products by the HPTLC fingerprint approach. Studies in Natural Products Chemistry, Elsevier."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1021\/jf0303924","article-title":"Antioxidant effect of ferulic acid in isolated membranes and intact cells: Synergistic interactions with alpha-tocopherol, beta-carotene, and ascorbic acid","volume":"52","author":"Trombino","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Serini, S., Cassano, R., Corsetto, P.A., Rizzo, A.M., Calviello, G., and Trombino, S. (2018). Omega-3 PUFA loaded in resveratrol-based solid lipid nanoparticles: Physicochemical properties and antineoplastic activities in human colorectal cancer cells in vitro. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19020586"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.carbpol.2019.01.001","article-title":"Xanthan gum-based materials for omega-3 PUFA delivery: Preparation, characterization and antineoplastic activity evaluation","volume":"208","author":"Trombino","year":"2019","journal-title":"Carb. Polym."},{"key":"ref_23","first-page":"1","article-title":"Screening of nutritional, phytochemical, antioxidant and antibacterial activity of Chenopodium album (Bathua)","volume":"3","author":"Pandey","year":"2014","journal-title":"J. Pharmacogn. Phytochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5050","DOI":"10.1021\/jf800421j","article-title":"Soil quality effects on Chenopodium album flavonoid content and antioxidant potential","volume":"56","author":"Chludil","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1002\/ptr.1243","article-title":"In vitro antioxidant activity of non-cultivated vegetables of ethnic Albanians in southern Italy","volume":"16","author":"Pieroni","year":"2002","journal-title":"Phytother. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"S178","DOI":"10.1016\/S2221-1691(12)60154-3","article-title":"Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein","volume":"2","author":"Chandra","year":"2012","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_27","first-page":"181","article-title":"Chemical constituents and anti-inflammatory activity of leaf essential oil of Nigerian grown Chenopodium album L.","volume":"9","author":"Usman","year":"2010","journal-title":"EXCLI J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1080\/14786419.2010.545356","article-title":"Comparative chemical composition and antioxidant activity of Calamintha nepeta (L.) Savi subsp. glandulosa (Req.) Nyman and Calamintha grandiflora (L.) Moench (Labiatae)","volume":"26","author":"Conforti","year":"2012","journal-title":"Nat. Prod. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"959","DOI":"10.2174\/187152012802649987","article-title":"Fig latex (Ficus carica L. cultivar Dottato) in combination with UV irradiation decreases the viability of A375 melanoma cells in vitro","volume":"12","author":"Menichini","year":"2012","journal-title":"Anticancer Agents Med. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1080\/14786419.2015.1020491","article-title":"A comparative study of Zingiber officinale Roscoe pulp and peel: Phytochemical composition and evaluation of antitumour activity","volume":"29","author":"Marrelli","year":"2015","journal-title":"Nat. Prod. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/cpr.12020","article-title":"Hypericum perforatum L. subsp. perforatum induces inhibition of free radicals and enhanced phototoxicity in human melanoma cells under ultraviolet light","volume":"46","author":"Menichini","year":"2013","journal-title":"Cell Prolif."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1080\/14786410500277761","article-title":"Antioxidant and cytotoxic activities of methanolic extract and fractions from Senecio gibbosus subsp. gibbosus (GUSS) DC","volume":"20","author":"Conforti","year":"2006","journal-title":"Nat. Prod. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3109\/13880200903029308","article-title":"Quantitative determination of Amaryllidaceae alkaloids from Galanthus reginae-olgae subsp. vernalis and in vitro activities relevant for neurodegenerative diseases","volume":"48","author":"Conforti","year":"2010","journal-title":"Pharm. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1007\/BF02535752","article-title":"Measurement of malondialdehyde by HPLC with fluorescence detection","volume":"25","author":"Tatum","year":"1990","journal-title":"Lipids"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.1271\/bbb.61.1942","article-title":"Antioxidant activity of ferulic acid beta-glucuronide in the LDL oxidation system","volume":"61","author":"Ohta","year":"1997","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"404","DOI":"10.3109\/14756366.2010.519336","article-title":"Chemical composition and protective effect of oregano (Origanum heracleoticum L.) ethanolic extract on oxidative damage and on inhibition of NO in LPS-stimulated RAW 264.7 macrophages","volume":"26","author":"Conforti","year":"2011","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1080\/14786419.2013.824440","article-title":"Synthesis of a new bis (indolyl) methane that inhibits growth and induces apoptosis in human prostate cancer cells","volume":"27","author":"Marrelli","year":"2013","journal-title":"Nat. Prod. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/s10787-017-0403-7","article-title":"Attenuation of nociceptive pain and inflammatory disorders by total steroid and terpenoid fraction of Euphorbia tirucalli Linn root in experimental in vitro and in vivo model","volume":"26","author":"Palit","year":"2018","journal-title":"Inflammopharmacology"},{"key":"ref_40","first-page":"272","article-title":"Anti-inflammatory activity of herbal plants: A review","volume":"2","author":"Kumar","year":"2013","journal-title":"Int. J. Adv. Pharm. Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"101","DOI":"10.3923\/rjmp.2011.101.115","article-title":"A review on medicinal plants as a source of anti-inflammatory agents","volume":"5","author":"Shah","year":"2011","journal-title":"Res. J. Med. Plant"},{"key":"ref_42","first-page":"122","article-title":"Anti-inflammatory activity and chemo profile of plants used in traditional medicine: A review","volume":"2","author":"Kushagra","year":"2010","journal-title":"J. Chem. Pharm. Res."},{"key":"ref_43","first-page":"1","article-title":"Plants in traditional medicinal system-future source of new drugs","volume":"1","author":"Rout","year":"2009","journal-title":"Int. J. Pharm. Pharm. Sci."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/8\/11\/505\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:34:36Z","timestamp":1760189676000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/8\/11\/505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,15]]},"references-count":43,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2019,11]]}},"alternative-id":["plants8110505"],"URL":"https:\/\/doi.org\/10.3390\/plants8110505","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,15]]}}}